• 한국해양공학회지
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• 제21권3호
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• pp.46-51
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• 2007

A multi-purpose environmental monitoring system has been developed as a commercially available standard using the technique of hetero-core spliced fiber optic sensors, for the purposes of monitoring large-scale structures and preserving natural environments. The monitoring system has been tested and evaluated in a possible outdoor condition, in view of the full-scale operation at actual sites to be monitored. Additionally, the developed system in this work conveniently provides us with various options of sensor modules intended for monitoring such physical quantities as displacement, distortion, pressure, binary states, and liquid adhesion. Two channels of optical fiber line were monitored in each channel, three displacement sensor modules were connected in series, in order to examine the performance to a pseudo-cracking experiment in the outdoor situation and to clarify temperature influences an the system, in terms of the coupling of optical connectors and the OTDR stability. The results from the pseudo-cracking experiment agreed with the actual cracks, by means of calculation, based an the detected displacement values and their geometrical arrangement of the used sensor modules. The temperature change, ranging from 10 to $20^{\circ}C$ resulting from the 10-days free running operation, was found to influence the system stability of ${\pm}10{\mu}m$, primarily due to the coupling instability of the used optical connectors. It was found that fusion splicing, rather than the use of connectors, reduced the fluctuation dawn to ${\pm}2{\mu}m$. The specification and performance of various option modules have been demonstrated to show the capability of inspecting various physical quantities by use of the single system, which would be suitable for multi-purpose environmental monitoring.

• 한국컴퓨터산업학회논문지
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• 제2권4호
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• pp.565-572
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• 2001

최근 고체 레이저 출력측에 비선형 광학재료를 설치하여 적외선에서 자외선에 이르기까지 넓은 대역의 파장을 가진 레이저광이 요구된다. 비선형 광학소자는 고조파발생기와 파라메터 발생기와 같은 레이저원을 이용한 주파수 전 영역까지 확장 할 수가 있다. 주파수 변환은 고전력 레이저를 이용한 확장기술에 많이 이용하고있다. 새로운 각 주파수 대역에서 광학 매개체의 비선형 광학의 응답 등을 이용할 수가 있다. 이러한 과정들은 자외선영역에서 적외선까지 고전력 방사발생을 이용할 수가 있다. 광학 파라메터발생기와 증폭기는 저주파수에서 2가지 파장 등을 발생한다. 싱글 주파수원으로부터 발생하여 몇몇의 경우에는 가시광선 영역에서 거의 자외선 영역까지 이용할 수가 있다. 결과적으로 녹색광을 얻기 위해서, 펄스형Nd:YAG 레이저는 다단펄스 포밍회로를 이용하였고 비선형광학(KTP)소자를 채택하여 적용하였다. 따라서 본 연구에서는 펄스 중첩법을 이용하여 기본파로서 직접 설계 제작 후 SHG 장치를 장착하여 녹색광을 얻고 각 중첩 메쉬에 같은 에너지를 인가했을 때의 레이저 출력과 녹색광 출력간의 상관관계와 메쉬 수에 따른 변환효율을 조사하였다.

• 한국전자통신학회논문지
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• 제14권6호
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• pp.1257-1264
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• 2019

기존의 Cuff 기반의 혈압(Blood Pressure)측정 방법은 연속적인 실시간 혈압측정에는 한계를 갖는다. 이러한 이유로 ECG와 PPG 센서 신호를 상호융합한 다양한 혈압추정이 이루어졌다. 그러나 PPG 중심에 측정기법은 AC 노이즈, 작은 맥동, 비박동 등의 많은 문제를 지니고 있다. 본 논문은 ECG와 PPG 관계에 발생하는 맥파전달시간(PTT)과 맥파속도(PWV)를 이용하여 혈압을 추론기법이다. 신호 피크를 이용하는 HRF(Height Ratio Features)에 비해, 본 제안방식은 ECG, PPG의 최고점 혹은 최저점을 사용한 시차를 이용해 추정하기 때문에 PPG 센싱 시그널의 오류에도 안정적인 추출이 가능한 장점이 있다. 본 논문에서 제안 방법을 이용하여 25만 회의 혈압측정의 결과 ±28.5%의 정확도를 갖는 혈압 추정기법을 제시할 수 있었다.

• Journal of Korean Neurosurgical Society
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• 제29권11호
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• pp.1469-1475
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• 2000

Objective : The goal of this study was to evaluate the clinical outcome of the posterior C1-2 transarticular screw fixation without C1-2 sublaminar wiring in atlantoaxial instability. Methods : Between Apr. 1995 and Feb. 2000, we used this technique in treat randomly selected 17 patients (11 men, 6 women) who had atlantoaxial instability. The causes of instability were : type II-A odontoid process fracture(10 cases) ; type II-P odontoid process fracture(1 case) ; Os odontoideum(2 cases) ; transverse ligament laxity due to rheumatoid disease(1 case) ; and, transverse ligament injury without bone fracture(3 cases). All cases were operated with posterior C1-2 transarticular screw fixation with 3.5mm cortical screw and interlaminar iliac graft without sublaminar wire fixation. The mean follow-up period was 28 months(5 to 58 months) and the mean age at the time of operation was 41 years(15 to 68 years). All Patients were allowed to ambulate with Philadelphia neck collar on the first post-operation day. Results : Bony fusion was successfully achieved in all cases demonstrated at 3-month follow-up studies. There was no operative mortality or morbidity. Conclusion : The authors conclude that the posterior transarticular screw fixation without C1-2 sublaminar wiring provide adequate stability with high bony union rate in atlantoaxial instability of various causes.

• 실천공학교육논문지
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• 제7권1호
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• pp.25-30
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• 2015

가상현실은 프로그래밍, 시뮬레이션, 컴퓨터 그래픽스 등 다양한 학문의 종합체이며 이러한 형태의 새로운 문화적 패러다임의 등장은 우리에게 새로운 개념의 콘텐츠 개발 방법과 정보교류방식을 요구하고 있다. 본 연구에서는 3D 가상현실 기술을 이용하여 I-Bench Mobile 이라는 새로운 장비를 통해 사용자와 인터렉티브가 가능한 가구배치 시뮬레이션을 제안한다. 이것은 3D로 제작이 되며 물리 및 중력을 적용한다는 점에 강점을 두며, 또한 스냅샷 기능을 통해 고객들이 가구를 자신이 원하는 장소에 배치하여 궁극적으로 고객 만족도 향상을 목표로 하고 있다. 가상증강현실기술을 이용한 소프트웨어를 개발하는 본 연구 과정은 공학계열의 컴퓨터학과와 예술계열의 영상 예술 디자인학과, 시각 디자인 학과와 같은 다양한 학과들에서 다루는 교육과정의 작업이 필요하므로 단지 코딩 기술이 아닌 여러 학문의 기술들이 함께 어우러진 융합 IT기술 교육의 좋은 사례라고 할 수 있다.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1997년도 춘계학술대회
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• pp.86-86
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• 1997

Human annexin I is a member of annexin family of calcium dependent phospholipid binding proteins, which have been implicated in various physiological roles including phospholipase A$_2$ (PLA$_2$) inhibition, membrane fusion and calcium channel activity. In this work, the structure of N-terminally truncated human annexin I (Δ-annexin I) and its interactions with Ca$\^$2+/, ATP and cAMP were studied at atomic level by using $^1$H, $\^$15/N, $\^$l3/C NMR (nuclear magnetic resonance) spectroscopy. The effect of Ca$\^$2+/ binding on the structure of Δ-annexin I was investigated, and compared with that of Mg$\^$2+/ binding. The addition of Ca$\^$2+/ to Δ-annexin I caused some changes in the high field and low field regions of $^1$H NMR spectra. Whereas, upon addition of Mg$\^$2+/ to Δ-annexin I, almost no change could be observed. Also we found that the binding ratio of ATP to Δ-annexin I is 1. Because Δ-annexin I is a large protein with 35 kDa molecular weight, site-specific (carbonyl-$\^$l3/C, amide-$\^$15/N) labeling technique was used to determine the interaction sites of Δ-annexin I with Ca$\^$2+/ and ATP. Assignments of all the histidinyl carbonyl carbon resonances have been completed by using Δ-annexin I along with its specific 1,2-subdomain. The carbonyl carbon resonances originating from His52 and His246 of Δ-annexin I were significantly affected by Ca$\^$2+/ binding, and some Tyr and Phe resonances were also affected. The carbonyl carbon resonances originating from His52 is significantly affected by ATP binding, therefore His52 seems to be involved in the ATP binding site of Δ-annexin I.

• 한국환경복원기술학회지
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• 제9권6호
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• pp.1-12
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• 2006

Remote sensing cannot provide a direct measurement of vegetation index (VI) but it can provide a reasonably good estimate of vegetation index, defined as the ratio of satellite bands. The monitoring of vegetation in nearby urban regions is made difficult by the low spatial resolution and temporal resolution image captures. In this study, enhancing spatial resolution method is adapted as to improve a low spatial resolution. Recent studies have successfully estimated normalized difference vegetation index (NDVI) using improved resolution method such as from the Moderate Resolution Imaging Spectroradiometer (MODIS) onboard EOS Terra satellite. Image enhancing spatial resolution is an important tool in remote sensing, as many Earth observation satellites provide both high-resolution and low-resolution multi-spectral images. Examples of enhancement of a MODIS multi-spectral image and a MODIS NDVI image of Cheongju using a Landsat TM high-resolution multi-spectral image are presented. The results are compared with that of the IHS technique is presented for enhancing spatial resolution of multi-spectral bands using a higher resolution data set. To provide a continuous monitoring capability for NDVI, in situ measurements of NDVI from paddy field was carried out in 2004 for comparison with remotely sensed MODIS data. We compare and discuss NDVI estimates from MODIS sensors and in-situ spectroradiometer data over Ochang plain region. These results indicate that the MODIS NDVI is underestimated by approximately 50%.

• 정보관리학회지
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• 제24권1호
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• pp.321-342
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• 2007

이 연구에서는 수작업 태깅없이 기계가독형 사전을 이용하여 자동으로 의미를 태깅한 후 학습데이터로 구축한 분류기에 대해 의미를 분류하는 단어 중의성 해소 모형을 제시하였다. 자동 태깅을 위해 사전 추출 정보 기반방법과 연어 공기 기반 방법을 적용하였다. 실험 결과, 자동 태깅에서는 복수 자질 축소를 적용한 사전 추출 정보 기반 방법이 70.06%의 태깅 정확도를 보여 연어 공기 기반 방법의 56.33% 보다 24.37% 향상된 성능을 가져왔다. 사전 추출 정보 기반 방법을 이용한 분류기의 분류 정학도는 68.11%로서 연어 공기 기반 방법의 62.09% 보다 9.7% 향상된 성능을 보였다. 또한 두 자동 태깅 방법을 결합한 결과 태깅 정확도는 76.09%, 분류 정확도는 76.16%로 나타났다.

• Journal of Microbiology and Biotechnology
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• 제25권11호
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• pp.1856-1862
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• 2015

In order to improve the stability of endoglucanase under thermal and acidic conditions, the endoglucanase gene was fused to the N-terminus of the Saccharomyces cerevisiae pir gene, encoding the cell wall protein PIR. The fusion gene was transformed into Pichia pastoris GS115 for expression. A resulting strain with high expression and high activity was identified by examining resistance to Geneticin 418, Congo red staining, and quantitative analysis of enzyme activity. SDS-PAGE analysis revealed that the endoglucanase was successfully displayed on the yeast cell surface. The displayed endoglucanase (DEG) showed maximum activity towards sodium carboxyl methyl cellulose at approximately 275 IU/g cell dry weight. DEG exhibited greater than 60% residual activity in the pH range 2.5-8.5, higher than free endoglucanase (FEG), which had 40% residual activity at the same pH range. The highest tolerated temperature for DEG was 70℃, much higher than that of FEG, which was approximately 50℃. Moreover, DEG showed 91.1% activity at 65℃ for 120 min, while FEG only kept 77.8% residual activity over the same period. The half-life of DEG was 270 min at 65℃, compared with only 150 min for FEG. DEG could be used repeatedly at least three times. These results suggest that the DEG has broad applications as a yeast whole-cell biocatalyst, due to its novel properties of high catalytic efficiency, acid-thermal stabilities, and reusability.

• Journal of Microbiology and Biotechnology
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• 제21권5호
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• pp.529-535
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• 2011

Single-chain variable fragment (scFv) is a fusion protein of the variable regions of the heavy (VH) and light (VL) chains of immunoglobulin, connected with a short linker peptide of 10 to about 20 amino acids. In this study, the scFv of a monoclonal antibody against the third domain of human CD4 was cloned from OKT4 hybridoma cells using the phage display technique and produced in E. coli. The expression, production, and purification of anti-CD4 scFv were tested using SDS-PAGE and Western blot, and the specificity of anti-CD4 scFv was examined using ELISA. A 31 kDa recombinant anti-CD4 scFv was expressed and produced in bacteria, which was confirmed by SDS-PAGE and Western blot assays. Sequence analysis proved the ScFv structure of the construct. It was able to bind to CD4 in quality ELISA assay. The canonical structure of anti-CD4 scFv antibody was obtained using the SWISS_MODEL bioinformatics tool for comparing with the scFv general structure. To the best of our knowledge, this is the first report for generating scFv against human CD4 antigen. Engineered anti-CD4 scFv could be used in immunological studies, including fluorochrome conjugation, bispecific antibody production, bifunctional protein synthesis, and other genetic engineering manipulations. Since the binding site of our product is domain 3 (D3) of the CD4 molecule and different from the CD4 immunological main domain, including D1 and D2, further studies are needed to evaluate the anti-CD4 scFv potential for diagnostic and therapeutic applications.