• The Plant Pathology Journal
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• v.29 no.1
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• pp.10-16
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• 2013

Hevea brasiliensis is a natural source of rubber and an important plantation tree species in Malaysia. Leaf blight disease caused by Fusicoccum substantially reduces the growth and performance of H. brasiliensis. The aim of this study was to use a combination of both morphological characteristics and molecular data to clarify the taxonomic position of the fungus associated with leaf blight disease. Fusicoccum species were isolated from infected leaves collected from plantations at 3 widely separated locations - Selangor, Perak, and Johor states - in Peninsular Malaysia in 2010. All the isolates were identified according to their conidial patterns and DNA sequences generated from internal transcribed spacers (ITS1 and ITS2), the 5.8S rRNA, and an unknown locus (BotF15) containing microsatellite repeats. Based on taxonomic and sequence data, Neofusicoccum ribis was identified as the main cause of leaf blight disease in H. brasiliensis in commercial plantations in Malaysia. A pathogenicity trial on detached leaves further confirmed that N. ribis causes leaf blight disease. N. ribis is an important leaf pathogen, and its detection in Malaysia has important implications for future planting of H. brasiliensis.

• Korean Journal Plant Pathology
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• v.3 no.1
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• pp.13-16
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• 1987

Infection rate of chestnut tree by Botryosphaeria(Dothiorella) dathidea was about $10\%$ at Gapyung area. disease symptom of chestnut did not appear in October but appeared in November, showing $50\%$ in May in ext year. B. dothidea was more pathogenic to chestnut than did Catinula sp. and Fusicoccum sp. B. dothidea uses soft rot, but Catinula sp. and Fusicoccum sp. causes dry rot. Mycelial growth of B. dothidea was optimal at $28^{\circ}C$. Mycelial growth was stimulated when water potential decreased from -2 bars to -12 bars and declined from -12 bars to -60 bars.

• Journal of Korean Society of Forest Science
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• v.96 no.5
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• pp.515-519
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• 2007

An imperfect fungus Fusiococcum species was isolated from Quercus dentata. A naturally infected Daimyo oak tree was collected and showed elongate wounds on the stem. The fungal cultures were initially white and cottony, and later turned dark gray. Numerous solitary pycnidia were developed on the medium surface, and typically spherical. Yellowish conidial masses were exuded from pycnidia on the culture plates. Conidial masses were swollen and measured as approximately 100 to $300{\mu}m$ in length. It appeared that conidia were usually held together in globose to oval drops. Conidia were hyaline, single-celled (nonseptate), ellipsoid to fusoid, and measured as approximately $8.0{\times}2.7{\mu}m$. Based on these cultural and morphological characteristics, the fungal isolate was identified as a species of Fusicoccum Corda. To preserve and examine fungal spores exuded from pycnidia on the medium surface, a vapor fixation procedure for scanning electron microscopy was employed in this study. The specimens were exposed to the vapor of 2% (v/v) glutaraldehyde and 2% (w/v) osmium tetroxide each for 2 h. With the vapor fixation we obtained excellent retention of conidial masses in this study. The simple and versatile procedure for demonstrating fungal spores and their exudation from fruiting bodies would facilitate characterization of diverse pathological and environmental isolates as they are in native environments.

• Korean Journal Plant Pathology
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• v.2 no.3
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• pp.174-184
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• 1986

Eleven fungi were isolated from the cankered stems, branchs and twigs of chestmut trees collected from Chuncheon, Gapyung, Wonseong, Inje and Kanghwa during 1985-1986. Among them, Botryosphaeria dothide (Moug. ex Fries) Ces. et. de Notaris, Cryptodiaporthe castanea (Tulasne) Wehmeyer, Endothia singularis Sheaet Stevens, E. parasitica (Murrill) P. J. et H. W. Anderson and Pseudovasella modonia (Tulasne) Kobayas were identified as perithecial stage. Dathiorella castaneae Camara et Vasconcelos, Fusicoccum castaneun Saccardo, Catinula japanica st. nov., Endothiella singularis (H. et. P. Syd.) Shear et Stev. nom. seminud, E. parasitica And., nom. seminud., and Coryneum castaneae (Sacc.) comb. novo were identified as conidial stage Botryosphaeria dothidea causing canker or diebark but and Endothia parasitica causing Endothia canker were already reported in Korea. The other fungi are first reported in Korea.