• Korean Journal of Environmental Agriculture
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• v.30 no.3
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• pp.261-267
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• 2011

ACKGROUND: Cadmium (Cd) has long been recognized as one of most toxic elements. Application of organic amendments and phosphate fertilizers can decrease the bioavailability of heavy metals in contaminated soil. METHODS AND RESULTS: This study was conducted to evaluate effect of combined application of phosphate fertilizer and manure in reducing cadmium phytoavailability in heavy metal contaminated soil. Phosphate fertilizers [Fused and super phosphate (FSP) and $K_2HPO_4$ (DPP)] and manure (M) were applied as single application (FSP, DPP, and M) to combined application (FSP+M and DPP+M) before radish seeding. $K_2HPO_4$ decreased $NH_4OAc$ extractable Cd and plant Cd concentration, mainly due to increases in soil pH and negative charge. However, FSP increased $NH_4OAc$ extractable Cd and plant Cd concentration. Manure significantly increased soil pH and negative charge. Combined application of phosphate fertilizer and manure were much more effective in reducing Cd phytoavailability than a simple application of each component. Calculated solubility diagram indicated that Cd concentrations in the solution of soils amended with phosphate fertilizers and manure were undersaturated with respect to all potential Cd minerals [$Cd_3(PO_4)_2$, $CdCO_3$, $Cd(OH)_2$, and $CdHPO_4$]. Plant Cd concentration and $NH_4OAc$ extractable Cd were negatively related to soil pH and negative charge. CONCLUSION: Alleviation of Cd phytoavailability with phosphate fertilizer and manure can be attributed primarily to Cd immobilization due to the increase in soil pH and negative charge rather than Cd and phosphate precipitation. Therefore, combined application of alkaline phosphate materials and manure is effective for reducing Cd phytoavailability.

• Korean Journal of Soil Science and Fertilizer
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• v.11 no.1
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• pp.31-36
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• 1978

A field experiment was conducted on a recently reclaimed hillside land to investigate the effectiveness of newly formulated compound fertilizer (10-14-11) containing fused phosphate as phosphorus source. Winter barley was adopted as a crop and commercial compound fertilizer (22-22-11), triple superphosphate, and ordinary fused phosphate were compared with the new compound fertilizer. The result of present study is summarized as follows. 1. When viewed from the plant hight and dry matter yield observed at different growth stages, it was indicated that newly formulated compound fertilizer seemed to be more effective in late stage than early stage. 2. Grain yields were higher in the compound fertilizer plots compared to the triple superphosphate and fused phosphate plots. The yield was specially high in the newly formulated compound fertilizer plot. In this plot, the number of grain per panicle and the rate of maturity were higher than the other plots. 3. The amount of phosphorus uptake by plant in the newly formulated compound fertilizer was lower in early stage and higher in late stage. In this experiment, the magnesium and silica contained in fused phosphate did not influence the growth and yield of barley because the soil was relatively high in these components. 4. Because of the unprecedented severity of winter weather during the experiment, the result obtained in present study may not be generalized until some studies confirm present findings.

• Korean Journal of Soil Science and Fertilizer
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• v.28 no.2
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• pp.176-182
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• 1995

Field and pot experiments were carried to find out the effects of direct application of phosphate rock powder on soybean plant in upland soils with different available P content. In newly reclaimed upland soil with extremely low available P, phosphate rock powder was superior to single superphosphate at the application rate of 400kg-600kg/ha, in the first year particularly. In the second year, however, the reverse was the case excepting at the rate of 200kg/ha. In upland soil with low avialble $P_2O_5$($52mg\;kg^{-1}$), the application of phosphate rock powder at the high appliaction rate(400-600kg/ha) increased the yield of soybean remarkably in the first year and there was residual effect in the second year. The application of phosphate rock at high rate, increased the P and Ca contenl of plant and soil. The concentration of all fractions of P in the soil at the harvest was higher in phosphate rock treatments than in single superphosphate and fused magnesium phosphate treatments. In this soil with low avaiable P the occurrence of different fractions of P was in the order of Org-P>Fe-P>Ca-P>Al-P, in the first year and there was no change in the second year.

• Journal of Embryo Transfer
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• v.9 no.1
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• pp.23-29
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• 1994

The long term goal of this research is to develop an efficient procedure for large scale production of genetically identical or cloned animals. To improve nuclear transpalntation efficiency in the rabbit, this study evaluated the age of nuclear recipient oocytes on the different steps of nuclear transplantation. The ovulated oocytes in different ages were collected from the superovulated does by flushing oviducts with Dulbecco's phosphate buffered saline(D-PBS) supplemented with 10% fetal calf serum(FCS) from 13 to 15, 17 to 20 and 23 to 26 hours after hCG injection. The denuded oocytes were used as nuclear recipient cytoplasm following enucleation by micromanipulation. The blastomeres separated from the 8-cell embryos were used as nuclear donor. The enucleated oocytes receiving a blastomere in the perivitteline space were fused in the 0.28 M mannitol solution at 1.5 kV/cm, 60 sec for three times. The fused oocytes were co-cultured with the monolayered rabbit oviductal epithelial cells in TGM-199 solution with 10% FCS for 72 hours at 37$^{\circ}C$ in a 5% $CO_2$ incubator. The cultured nuclear transplant embryos and in vivo developed embryos collected at 72 hours after hCG injection were stained with Hoechst 33342 dye. Their cell numbers were counted under a fluorescent microscope. The results obtained were summarized as follows ; 1. The aged oocytes(20 hrs. post hCG) showed significantly(P<0.05) higher fusionrates(70 ~ 90%) than the recently ovulated oocytes(30.8%) 2. The aged oocytes which were electrically activated and fused at 20 hours developed to blastocyst at significantly(P<0.05) high rate, while none of the recently ovulated oocytes developed to blastocyst. 3. Even though the aged oocytes at 23~26 hours showed higher fusion rate(85.7%), not only they were inadequate to manipulate but also their developmental potential to blastocyst was highly impaired. 4. The developmental potential in vitro of nuclear transplant embryos was significantly retarded than in vivo deveolped embryos.

• Korean Journal of Environmental Agriculture
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• v.17 no.2
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• pp.182-188
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• 1998

The effect of arbuscular mycorrhizae(AM) on the growth of trifoliate orange seeding were investigated in volcanic ash soil. Trifoliate orange is used as a root stock of citrus in Cheju island. Seedings innoculated with AM fungi were grown for 16 weeks in pots of various levels of fertilizer. Growth characteristics and mineral nutrient contents were measured and spores of AM fungi colonized were identified. Seventy % of the replicsted pots of seedings were colonized by AM in the treatment of high level fertilizer and additional phosphate (40g of 21-17-17 complex fertilizer and 50g of fused phosphate added to 50L of soil). In treatments of low levels fertilizer or without fused phosphate addition, the pots colonized were less than 20 %. Colonization of trifoliate seedings with AM fungi greatly increased the growth of seedings. Shoot length and weight of shoot and root positively regressed on AM colonization ratio. AM colonization caused higher concentrations of P, Cu and Mg in plant, and the relations were significant at 5 % level. Contents of N and Zn in plants also tended to increase, while that of Ca to decrease, with increasing colonization ratio. Four species of AM fungi - Glomus deserticola, G. rubiforme, G. vesiculiferum and Acaulospora sp, - were found in the soil where roots of trifoliate orange as an innoculation materials were collected. All of the 4 species were found in the inoculated pot soils after the seedling growth, indicating that these species can be colonized in trifoliate orange roots.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.3
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• pp.167-170
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• 2000

This study was conducted to investigate the changes of potato growth and soil properties in highland mounding-soil. Experimental plot was designed with control, chemical improvement and zeolite+chemical improvement plots. Mounding-soil was low in organic matter and phosphate contents, and that soil texture was loamy sand soil. The growth and yield of potato were highest in the zeolite treatment plot. Yield of that increased by 3.7% than control plot. Increasing ratios of potato tubers decreased at 60-70 days after planting. Increasing ratio of zeolite plot was higher or than other treatment plots. After harvesting, the contents of soil organic matter increased by 12-125%, and those of phosphate were by 29-73% respectively. The manurial value of fused and superphosphate was higher than that of potassium chloride.

• Journal of Microbiology
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• v.37 no.3
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• pp.141-147
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• 1999

Thirteen transcriptionally-fused carbon starvation mutants, derived from Pseudomonas putida ATCC 12633, were analyzed for their survivability and transcriptional induction profiles upon carbon starvation. One of these mutants, MK114, which exhibited the lowest survivability and the highest induction rate, was selected and further examined under different starvation (nitrogen and phosphate) and stress (osmolarity, H2O2, salts, alcohol, and heat) conditions. Under all tested conditions MK114 induced ${\beta}$-galactosidase activity, implying that the interrupted gene (cst114) is a general starvation and stress response gene. The rate of induction ranged from 2.6-fold for phosphate starvation to 3.7-fold for osmotic shock. The mini-Tn5 flanking DNA was cloned from the chromosome of MK114. The cloned DNA fragment exhibited carbon starvation activity, indicating that this fragment contains a carbon starvation-related promoter region. This region was partially sequenced. Possible physiological roles of Cst114 in a carbon sensing mechanism and in other stress responses are also discussed.

• Korean Journal of Soil Science and Fertilizer
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• v.21 no.4
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• pp.426-433
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• 1988

A pot experiment was conducted to find out the effects of application of slacked lime and fused super-phosphate on the lead uptake of upland crops in a lead added soil. Lead concentration of the soils were adjusted to 0, 150, 300mg/kg respectively. The slacked lime was applied at the equivalent amount of lime requirement with extra 150kg/10a, and 2 times for the fused superphosphate. The results obtained were as follows: 1. Lead contents in crops increased in the order: sesame > maize > potato > sweet potato > soybean > green perilla > peanut > red bean. 2. Lead contents in parts of crops were increased in the order; root > stem > leaf > grain. 3. Increasing lead concentration in soils, lead content in the plant was increased and crops yield were decreased. 4. Lead contents in soybean and green perlilla were decreased in slacked lime application treatment. 5. The lead contents in leaf and grain of soybean and green perllila decreased with decreasing in the ratio of Pb/Ca+Mg equivalent in soil. 6. Grain yield were increased in slacked lime, but were decreased in fused superphosphate application treatment. 7. With increasing the soil Pb contents, calcium and phosphate contents were increased in leaf and stem, but calcium was decreased in roots. 8. $1N-NH_4$ OAC soluble Pb contents in soil were 26-50 ppm and 42-70 ppm, respectively, for 150mg/kg and 300mg/kg lead treatments. 9. The soil pH was increased in the order of slacked lime, fused superphosphate and nontreatment.

• BMB Reports
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• v.41 no.5
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• pp.408-413
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• 2008

Pyridoxal-5'-phosphate phosphatase (PLPP) catalyzes the dephosphorylation of pyridoxal-5'-phosphate (PLP). A human brain PLPP gene was fused with a PEP-1 peptide and produced a genetic in-frame PEP-1-PLPP fusion protein. The purified PEP-1-PLPP fusion protein was efficiently transduced into PC12 cells in a time- and dose-dependent manner when added exogenously to culture media. Once inside the cells, the transduced PEP-1-PLPP fusion protein was stable for 36 h. The concentration of PLP was markedly decreased by the addition of exogenous PEP-1-PLPP to media pretreated with the vitamin $B_6$ precursors; pyridoxine, pyridoxal kinase and pyridoxine-5'-phosphate oxidase into cells. The results suggest that the transduction of the PEP-1-PLPP fusion protein can be one mode of PLP level regulation, and to replenish this enzyme in the various neurological disorders related to vitamin $B_6$.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.6
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• pp.669-676
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• 2016

A field study was conducted to determine the runoff loss of N and P in small scale of red pepper field plots (10% slope), consisting of three different plots with black polyethylene vinyl mulching (mulching), ridge without mulching (ridge), and flat without ridge and mulching (flat). Composted manure and urea as a basal application were applied at rates of $20MT\;ha^{-1}$ and $93kg\;N\;ha^{-1}$, respectively. Urea at $189kg\;N\;ha^{-1}$ and fused phosphate at $67kg\;P_2O_5\;ha^{-1}$ were additionally applied on June 25 with different fertilization methods, broadcast application in flat plot and hole injection in ridge and mulching plots. Plant uptake of N and P was positively correlated with their respective concentrations in surface soil: mulching > ridge > flat plots. The runoff loss by soil erosion was higher in flat plot than ridge and mulching plot with contour line. Nitrate loss by the runoff water had no significant differences among three surface management practices, but the higher average value in ridge and mulching plots than flat plot. Especially, the flat plot had no phosphate loss during summer season. This is probably due to low labile P content in surface soil of flat plot. In the summation of soil and water loss, flat plot was higher in N and P loss than ridge and mulching plot with contour line. Nevertheless, the nitrate and phosphate loss by runoff water could be more important for non-point source management because the water could meet the river easier than eroded soil because of re-deposition around slope land.