• 제목/요약/키워드: Fungi isolate

검색결과 221건 처리시간 0.032초

Molecular and Morphological Characterization of Endophytic Heterobasidion araucariae from Roots of Capsicum annuum L. in Korea

  • Paul, Narayan Chandra;Deng, Jian Xin;Shin, Kyu-Seop;Yu, Seung-Hun
    • Mycobiology
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    • 제40권2호
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    • pp.85-90
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    • 2012
  • A species of Heterobasidion was encountered during a diversity study of endophytic fungi from healthy root tissues of chili pepper (Capsicum annuum L.) in Korea. The fungal species (CNU081069) was identified as Heterobasidion araucariae based on phylogenetic analyses of the internal transcribed spacer and translation elongation factor gene sequences. Morphological descriptions of the endophytic isolate matched well with the previous references and supported the molecular identification. The fungus Heterobasidion araucariae CNU081069 is new to Korea.

Isolation, Physico-chemical Properties, and Biological Activity of New Thiopeptide Antibiotics, Kimorexins

  • Yeo, Woon-Hyung;Kim, Si-Kwan;Kim, Sang-Seock;Yu, Seung-Hun
    • Journal of Microbiology and Biotechnology
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    • 제4권4호
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    • pp.349-353
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    • 1994
  • An isolate 90-GT-302, identified as Kitasatosporia kimorexae, was found to produce antibiotics that induce mycelial swelling in Magnaporthe grisea, and Fusarium solani. The strain produced at least 5 antibiotics. Among them, the main active compound designated as kimorexin A was isolated and its physico-chemical properties and biological activities were examined, and as a result was found to be of the thiopeptide antibiotic. A comparison between the properties of kimorexin A and those of the known thiopeptide antibiotics led us to conclude that kimorexin A was a new thiopeptide polythiazolyl antibiotic. Kimorexin A showed a narrow antimicrobial spectrum against very limited genus of phytopathogenic fungi. It prevented host plants from infections of Rhizoctonia solani and absolute parasitic fungi, such as Sphaerotheca fuliginea and Puccinia recondita, almost completely at the treatment concentration of approximately 20 ppm.

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내생균 Arthrinium phaeospermum이 생산하는 이차대사산물 (Secondary Metabolites Produced by Endophytic Fungus, Arthrinium phaeospermum)

  • 하설규;심상희
    • 생약학회지
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    • 제47권3호
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    • pp.217-221
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    • 2016
  • Endophytic fungi have yielded a variety of secondary metabolites so far. In the course of the project to find bioactive secondary metabolites from cultures of endophytic fungi, an isolate of Arthrinium phaeospermum (JS 0567) was selected for chemical investigation. A large scale culture of this strain in rice media was extracted with an organic solvent and the extract was subjected to a serious of chromatography, which led to six metabolites. Their chemical structures were elucidated as 2,3,6,8-tetrahydroxy-1-methylxanthone(1), 2,3,4,6,8-pentahydroxy-1-methylxanthone(2), 3,4,6,8-tetrahydroxy-1-methylxanthone(3), 3,6,8-trihydroxy-1-methylxanthone(4), 2,4,2',4',6'-pentahydroxy-6-methylbenzophenone(5), and 5,7-di hydroxy-3-methylphthalide(6) on the basis of spectroscopic data. To the best of our knowledge, this is the first study on the secondary metabolites from Arthrinium phaeospermum.

전기영동법(Disc)에 의한 고등균류의 몇개 종간에 있어서 단백질 및 효소의 비교 (Electrophoretic Comparison of Mycelial Protein and Enzyme Patterns in Three Interspecies of Some Edible Fleshy Fungi)

  • 홍순우;박민철
    • 미생물학회지
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    • 제12권3호
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    • pp.138-146
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    • 1974
  • Taxonomic relations among three species of some edible fleshy fungi(Lentinus edodes, Pleurotus ostreatus, Flammulina velutipes) in the family Tricholomataceae were examined by using polyacrylamied gel disc electrophoresis. The soluble, crude extract of mycelium grown on potato sucrose broth was subjected to electrophoresis. Similarities in the protein bands for each isolated of one species were compared with those for others. In the banding patterns there was a closer relationship between isolates within one species than among isolates of different species. However, the isozyme patterns obtained from each isolate of Peurotus ostreatus (esterase, peroxidase, tyrosinase) were appeared to represent the degree of geographical variability within one species.

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Screening of Taxol-producing Endophytic Fungi from Ginkgo biloba and Taxus cuspidata in Korea

  • Kim, Soo-Un;Strobel, Gary;Ford, Eugene
    • Journal of Applied Biological Chemistry
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    • 제42권2호
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    • pp.97-99
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    • 1999
  • Endophytic fungi from Ginkgo biloba and Taxus cuspidata in Korea were screened for production of taxol. Eighteen and twelve fungal isolates from G. biloba and T. cuspidata, respectively, were shown to produce immunologically detectable amount of taxol. The highest production of taxol at 260 ng/l was achieved by stationary culture of an Alternaria isolate from G. biloba. The strain also produced unidentified antifungal agent(s) against Pythium ultimum. However, the activity gradually decreased when the strain was stored at $4^{\circ}C$ for 6 months.

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Compatibility of Entomopathogenic fungus Lecanicillium attenuatum and Pesticides to control Cotton Aphid, Aphis gossypii

  • Kim, Jeong-Jun;Kim, Kyu-Chin
    • International Journal of Industrial Entomology and Biomaterials
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    • 제14권2호
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    • pp.143-146
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    • 2007
  • Concerns of entomopathogenic fungi as alternative pest control agents are increasing even though chemical pesticides have been used as the main control agents for pests and diseases in crop production. This study was conducted to test the influence of fungicides and insecticides on an isolate of Lecanicillium attenuatum that was reported to have the pathogenicity against cotton aphid, because fungicides and/or insecticides can apply with mycopesticides simultaneous, before and/or after. Fungicides fenbuconazole+thiram and propineb inhibited the spore germination and mycelial growth of L. attenuatum CS625; dimethomorph and procymidone did not affect spore germination or mycelial growth. The insecticide abamectin, deltamethrin, imidachropride, and spinosad had no detrimental effects on spore germination or mycelial growth. Therefore, these results demonstrated that careful selection of pesticides and fungicides can be applied to the integrated pest and disease control with microbial pesticide.

Paenibacillus polymyxa and Burkholderia cepacia Antagonize Ginseng Root Rot Pathogens

  • Lee, Young Don;Hussein, Khalid Abdullah;Joo, Jin Ho
    • 한국토양비료학회지
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    • 제50권6호
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    • pp.598-605
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    • 2017
  • To isolate rhizobacteria exhibiting antifungal activities for for five pathogenic fungi (Sclerotinia sclerotiorum, Fusarium solani, Collectotricum gloeosporides, Fusarium oxysporum, and Botrytis cinerea) which cause damage to Ginseng root in Ginseng grown fields, four soils were collected from Cheorlwon gun, in Korea. From 4 soils, a total of 160 bacterial strains were isolated by dilution plate method. Among 160 strains, 32 strains showed antifungal activities for one or more pathogens. From 32 strains, three strains exhibited antifungal activities for all pathogens. These are two Burkholderia cepacia (ATCC 25416 and ET 13) and one Paenibacillus polymyxa (ATCC 842). These potent antifungal strains showed high identities (99% using 16S-rRNA sequencing).

닭의난초(Epipactis thunbergii)에 공생하는 난 균근균의 분리 및 동정 (Identification of Orchid Mycorrhizal Fungi Isolated from Epipactis thunbergii in Korea)

  • 한한결;정재민;조용찬;김대신;엄안흠
    • 한국균학회지
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    • 제41권1호
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    • pp.9-13
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    • 2013
  • 제주도의 북쪽에 위치한 추자도에서 닭의난초 뿌리를 채취하였고, 표면 살균한 뿌리에서 6개의 균주를 분리하였다. 각 균주는 형태적 특징을 기초로 그룹을 나누었고, 난 균근균에 특이적인 프라이머인 ITS1-OF/ITS4-OF를 이용하여 균주의 rDNA의 ITS지역을 증폭하였다. 형태적 특징과 분자적 분석을 통하여 6개의 균주를 Sebacina sp., Tulasnella calospora, Tulasnella sp. 총 3종의 난 균근균으로 동정하였다.

Aphelenchus avenae and Antagonistic Fungi as Biological Control Agents of Pythium spp.

  • Jun, Ok-Kyoung;Kim, Young-Ho
    • The Plant Pathology Journal
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    • 제20권4호
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    • pp.271-276
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    • 2004
  • To examine the control effect of damping-off on radish caused by Pythium spp., researchers used the isolates of a fungivorous nematode, Aphelenchus avenae, and antagonistic fungi, Trichoderma spp. These were used as biocontrol agents, either alone, or in combination. Growth rates of the A. avenae isolates and fungal damages by the nematodes varied depending on Trichoderma spp., which contained lower T. koningii and T. virens cultures than other Trichoderma cultures. Phythium spp. were damaged by all five Aphelenchus isolates, but the multiplication rate of nematode isolate Aa-3 was very poor. Antibiotic activity of T. virens and T. harzianum to Pythium spp. was stronger than that of T. koningii. Control efficacy against damping-off of radish was most enhanced under the treatment using the nematode-T. harzianum combination. On the contrary, the combinations of the nematodes and T. virens or T. koningii mostly did not increase or decreased their control effect vis-$\`{a}$-vis that of the nematodes or antagonistic fungi being used alone. The results suggest that the fungivorous nematodes may play a leading role in the disease control, and that the activity of the fungivorous nematodes may be activated by T. harzianum, but inhibited by T. koningii and T.virens.

쇠비름 즙액(汁液)의 항균작용(抗菌作用) (Antifungal Activity of Extract of Common Purslane (Portulaca oleracea L.))

  • 박종성;권진숙;이규승
    • 농업과학연구
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    • 제11권2호
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    • pp.190-193
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    • 1984
  • 쇠비름즙액(汁液)(원액(原液))은 사과 부란병균(病菌)(Valsa mali), 배 검은무늬병균(病菌)(Alternaria kikuchiana), 벼 도열병균(病菌)(Pyricularia oryzae)등 몇가지 식물병원균(植物病原菌)에 대한 항균작용(抗菌作用)을 지니고 있다. 이와 같은 항균작용(抗菌作用)을 나타내는 물질(物質)은 메타놀에 잘 용해(溶解)되며 지질(脂質)에 속하는 물질(物質)로 추정(推定)된다. 그리고 쇠비름즙액(汁液)에 함유(含有)되어 있는 항균물질(抗菌物質)은 균채신장억제(菌采伸長抑制) 뿐만 아니라 포자발아억제(胞子發芽抑制) 효과(效果)를 보여주고 있다.

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