• Title/Summary/Keyword: Fungal rDNA

Search Result 278, Processing Time 0.024 seconds

Unreported Endophytic Fungi Isolated from the Leaves of Evergreen Woody Plants in Korea: Muyocopron lithocarpi and Didymocyrtis cladoniicola

  • Gwon, Ju-Hui;Park, Hyeok;Lee, Jong-Chul;Choi, Jae-Wook;Eom, Ahn-Heum
    • The Korean Journal of Mycology
    • /
    • v.49 no.3
    • /
    • pp.373-378
    • /
    • 2021
  • In this study, endophytic fungi were isolated from the leaves of evergreen woody plants in Hansando Island in South Korea. The fungal isolates were identified based on morphological characteristics and phylogenetic analysis of the DNA sequences of the internal transcribed spacer and the large subunit regions of rDNA. It was confirmed that two endophytic fungal species, Muyocopron lithocarpi and Didymocyrtis cladoniicola, isolated in this study have not previously been reported in South Korea.

Three Unreported Endophytic Fungi Isolated from Conifer Leaves of Pinus densiflora in Korea (소나무 침엽에서 분리된 3종의 국내 미기록 내생균)

  • Park, Hyeok;Eom, Ahn-Heum
    • The Korean Journal of Mycology
    • /
    • v.47 no.1
    • /
    • pp.35-42
    • /
    • 2019
  • We isolated endophytic fungi from the conifer leaves of Pinus densiflora inhabiting Buan-gun, Jeollabuk-do, Korea. We identified the isolated fungal strains based on phylogenetic analysis performed using the nucleotide sequences of the internal transcribed spacer, large subunit, and beta-tubulin. We confirmed the presence of three novel endophytic fungi in Korea, namely Paracamarosporium hawaiiense, Tubakia dryina, and Zasmidium fructigenum. In this report, we described the morphological characteristics of these fungal strains and the results of their phylogenetic analysis.

Isolation and Characterization of Previously Undescribed Seventeen Fungal Species Belonging to the order Hypocreales in Korea

  • Jaeduk Goh;Yoosun Oh;Young-Hwan Park;Hye Yeon Mun;Sangkyu Park;Wonsu Cheon
    • The Korean Journal of Mycology
    • /
    • v.50 no.1
    • /
    • pp.1-29
    • /
    • 2022
  • Hypocreales is one of the largest orders within the class Sordariomycetes in Ascomycota. Several species of this order are cosmopolitan and have a broad range of habitats. Here, we isolated several fungal strains from environmental samples, including freshwater sediment and plant litter. The strains were identified via molecular and phylogenetic analyses of rDNA and other DNA markers, such as TUB, RPB2, and EF1. The morphological characteristics of the fungi were investigated using microscopy, and culture characteristics were assessed from their growth on several media. We identified 17 species previously unrecorded in Korea: Dactylonectria hordeicola, Flavocillium bifurcatum, Fusarium luffae, Ilyonectria ilicicola, Ilyonectria qitaiheensis, Ilyonectria robusta, Lecanicillium aphanocladii, Nectria ulmicola, Neonectria lugdunensis, Ovicillium oosporum, Pseudonectria foliicola, Sarocladium spinificis, Scolecofusarium ciliatum, Trichoderma appalachiense, Trichoderma subviride, Trichoderma taiwanense, and Trichoderma tsugarense.

Identification of Unrecorded Endophytic Fungi Isolated from Leaves of Woody Plants in Jejudo, Korea (제주도에 서식하는 목본 식물의 잎에서 분리한 미기록 내생균)

  • Lee, Bong-Hyung;Kim, Dong-Yeo;Park, Hyeok;Eo, Ju-Kyeong;Lee, Hyang Burm;Eom, Ahn-Heum
    • The Korean Journal of Mycology
    • /
    • v.44 no.4
    • /
    • pp.252-258
    • /
    • 2016
  • In this study, endophytic fungi were isolated from the leaves of five species of woody plants in Jeju, Korea, namely Chamaecyparis obtusa, Cryptomeria japonica, Torreya nucifera, Ilex crenata, and Camellia japonica. The isolated fungal endophytes were identified based on their morphological and molecular characteristics including a sequence analysis of the internal transcribed spacer and 26S regions of rDNA and ${\beta}$-tubulin genes. Ten species of fungal endophytes have not been previously reported in Korea, namely Mycosphaerella aleuritidis, Neofusicoccum eucalyptorum, Neofusicoccum parvum, Phyllosticta citrichinensis, Phyllosticta cryptomeriae, Phomopsis cotoneastri, Sphaerulina rhododendricola, Guignardia mangiferae, Lophodermium jiangnanense, and Lophodermium minus.

First Reports of Unrecorded Mortierellomycetes and Umbelopsidomycetes Fungi from Freshwater Ecosystems in Korea

  • Jaeduk Goh;Yoosun Oh;Hye Yeon Mun
    • The Korean Journal of Mycology
    • /
    • v.51 no.3
    • /
    • pp.155-165
    • /
    • 2023
  • In this study, we isolated several fungal strains from filtered water and sediment collected from rivers and streams. The strains were identified by molecular phylogenetic analyses of rDNA sequences (internal transcribed spacer [ITS], large subunit of ribosomal DNA [LSU]). The morphological characteristics of the fungi were investigated using microscopy, and the culture characteristics of fungi grown on several media were examined. We identified four species previously unknown in South Korea, namely, Dissophora globulifera, Linnemannia exigua, Mortierella rishikesha and Umbelopsis autotrophica.

Unrecorded Fungal Species Isolated from Medicinal Plant Leaves in Korea (약용식물 잎에서 분리한 국내 미기록 진균)

  • Ahn, Geum Ran;Noh, Hyeungjin;Lee, Dong Hyeung;Kim, Susan;Kim, Jun Young;Kim, Seong Hwan
    • The Korean Journal of Mycology
    • /
    • v.47 no.4
    • /
    • pp.319-328
    • /
    • 2019
  • As an effort to explore fungal diversity, fungal survey was undertaken in 2018 in Jangheung, Korea. For the survey, medicinal plant leaves were sampled from Selaginella tamariscina, Serratula coronata ssp. insularis, Scutellaria baicalensis, Scrophularia buergeriana and subjected to fungal isolation. Four unrecorded fungal species, Paraboeremia selaginellae, Colletotrichum camelliae, Alternaria eichhorniae, and Phomopsis capsici were obtained from the survey. This study described their morphological characteristics including colony features formed on media, light microscopic images and molecular characteristics of nucleotide sequences of the ITS and 28S rDNA regions.

First Reports of Five Endophytic Fungi Isolated from Leaves of Plants Inhabiting the Hansando Island in Korea (한산도에 서식하는 식물의 잎에서 분리된 5종의 국내 미기록 내생균)

  • Park, Hyeok;Lee, Jong-Chul;Eom, Ahn-Heum
    • The Korean Journal of Mycology
    • /
    • v.48 no.3
    • /
    • pp.217-228
    • /
    • 2020
  • Endophytic fungi were isolated from the leaves of plants inhabiting the Hansando Island in Korea. We identified the fungal strains based on their morphological characteristics and on the phylogenetic analysis using DNA sequences of the internal transcribed spacer, small subunit rDNA, large subunit rDNA, and translation elongation factor 1- α regions. In this study, we isolated five endophytic fungi that have not been recorded in Korea, Arthrinium camelliaesinensis, Beltraniella humicola, B. portoricensis, Microxiphium theae, and Piceomphale pinicola. Their morphological characteristics as well as the results of molecular phylogenetic analysis are reported here.

Five Species of Endophytic Fungi Isolated from Roots of Native Orchid Plants from Korea (한국 자생 난초 뿌리에서 분리한 미기록 내생균 5종)

  • Lee, Bong-Hyung;Eom, Ahn-Heum
    • The Korean Journal of Mycology
    • /
    • v.45 no.4
    • /
    • pp.355-361
    • /
    • 2017
  • In this study, endophytic fungi were isolated from the roots of three native orchid species from Korea: Bletilla striata, Oreorchis patens, and Cephalanthera longibracteata. The isolated fungal endophytes were identified based on the morphological and molecular characteristics including sequence analysis of internal transcribed spacer (ITS) and 28S ribosomal DNA regions. As a result, we discovered 5 species of fungal endophytes that have not been previously reported in Korea: Phialocephala bamuru, Coniochaeta mutabilis, Phialophora foetens, Calonectria canadensis, and Neonectria ramulariae.

Two Unreported Species of Endophytic Fungi Isolated from Leaves of Abies koreana in Korea (구상나무의 잎에서 분리한 두 종의 국내 미기록 내생균)

  • Park, Hyeok;Kim, Dong-Yeo;Eom, Ahn-Heum
    • The Korean Journal of Mycology
    • /
    • v.46 no.1
    • /
    • pp.22-27
    • /
    • 2018
  • We isolated endophytic fungal strains from the leaves of Abies koreana growing in Korea. The fungal strains were identified as Coleophoma parafusiformis and Coniochaeta ligniaria, based on morphological characteristics and sequence analysis of the internal transcribed spacer region and large subunit region of ribosomal DNA. These species have not been previously reported in Korea. In this study, we report the morphological characteristics and results of phylogenetic analysis of the two novel fungal strains.

Identification of Rhizo-bacterium Inhibiting Diaporthe citri Causing Citrus Melanose (감귤 검은점무늬병균의 생육을 저해하는 근권 세균의 분리 및 동정)

  • Nnam, Myung-Hyeun;Shin, Jin-Ho;Choi, Jae-Pill;Hong, Suck_Il;Kim, Young-Gwon;Kim, Heung-Tae
    • The Korean Journal of Pesticide Science
    • /
    • v.13 no.4
    • /
    • pp.332-335
    • /
    • 2009
  • Rhizo-bacteria were isolated from organic-farming soils to select antagonistic agent for controlling citrus melanose disease. Among several antagonistic bacteria, KB-401 effectively inhibited mycelial growth of several plant fungal pathogens, including the pathogen of citrus melanose, Diaporthe citri. KB-401 also inhibited spore germination of the fungal pathogen. The tip of germ tube was swollen when conidia of D. citri were co-culture with KB-401 in PD broth amended 1% glucose. KB-401 was identified as Bacillus subtilis through the investigation for physiological characters and the analysis of nucleotide sequences of 16S rDNA.