• Journal of Forest and Environmental Science
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• v.15 no.1
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• pp.77-88
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• 1999

This experiment was carried out to compare the cultural characteristics of mycorrizal fungi associated with Pinus species, and to form mycorrhizal association with Pinus thunbergii by artificial inoculation of these fungi. Mycorrhizal fungi tested showed great variations in cultural characteristics. Most fungal isolates was best grown on MP medium, except PDA for Lepista sp.(Ln73/92). Hagem for Rhizopogon rubescens(FRI91017), and FDA for Paxillus sp.(Pa60/92). Optimum temperature for these fungi was $25^{\circ}C$, except $30^{\circ}C$ for Pisolithus tinctorius(FRI91004 and Pt1). The range of pH conditions favorable for these fungal isolates were also variable from weak acidic(pH5) to weak alkalic(pH8). Utilization of the carbon sources for these mycorrhizal fungi was different. Fructose, glucose, and maltose were all utilized well, while xylose was not utilized generally. Mycelial growth on the media supplemented with potassium nitrate was better than those on other media with urea, asparagine, or peptone as a nitrogen source, and the poor growth was observed on the media with urea. Pisolithus tinctorius(Pt1) among 7 mycorrhizal fungi artificially inoculated for the mycorrhizal synthesis on pinus thunbergii seedlings in the test tube containing a mixture of peat moss-vermiculite(2:1, v/v) formed mycorrhizae successfully after 3 months. P. tinctorius formed branched and unbranched roots covered with thick fungal mantle and radiating extemal hyphae. Mycorrhizal root cross-sectioned by hand, stained, and observed by Nomarski interference microscope showed typical characteristics of ectomycorrhizae: fungal mantle on epidermal cells and thick Hartig net hyphae around cortex cells.

• Journal of Korean Society of Forest Science
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• v.94 no.1 s.158
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• pp.16-20
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• 2005

Tricholoma matsutake ectomycorrhizas are unique in their morphology: not bifurcated broom shaped roots with not easily wettable brilliant and profuse white hyphae. To understand these characteristics the ectomycorrhizas were investigated with electron microscopy. T. matsutake ectomycorrhiza have thin mantle and typical Hartig net development in the epidermis and cortex, but no fungal mantle on the root apex. There were no penetrating hyphae inside of the cells of either epidermis, cortex or stele. Inside of the walls of epidermis and cortex cells are lined with ca. $2{\mu}m$ hemispherical amyloplasts. The brilliant hyphal surface was covered with various fine amorphous granules. The hyphal cell wall was thin membrane less than $0.3{\mu}m$ thick. There is no clamp connection on the hyphae. This thin membraneous cell wall with high elasticity can be related to survival strategy of the species without plasmolysis under frequent soil water stress environment. And the coarse hyphal surface with some water repellency can control sudden inrush of water of the hyphae with an extremely low osmotic potential. It is concluded that no mantle on the tip can induce mycorrhizas not bifurcated and that finely coarse surface of T. matsutake hyphae can make the hyphae brilliantly white but less wetted.

• Mycobiology
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• v.31 no.1
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• pp.1-8
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• 2003

Detailed structures of ectomycorrhizae formed in Pinus roots were observed with various microscopes: light, fluorescence, and scanning electron microscopes. The mantles and Hartig nets commonly found in the structure of ectomycorrhiza were newly observed according to developmental stage by various staining. The mycelia were observed to be composed of coiled types on the surface of epidermal root during early stage and fused to form mantles of smooth fungal layers, loosing mycelia with some viscous liquid secreted. The ectomycorrhizal hyphae in anatomical roots penetrated the cortical layer and formed obviously mantle and Hartig net. The round spots of ectomycorrhizal mycelia were observed morphological distribution from the cortical layer to vascular bundle of stele in the ectomycorrhizal roots of Pinus species and especially scattered at the area of meristem at the root tip as longitudinal sections. Those mycelia penetrated seemed to move into other roots by means of vascular bundle of ectomycorrhizal roots and newly form ectomycorrhizal roots of dichotomous branches.

• Journal of Korean Society of Forest Science
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• v.89 no.3
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• pp.389-396
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• 2000

To determine whether the Tricholoma matsutake (pine mushroom, Songyi) is symbiotic or parasitic to Pinus densiflora, structural change in their natural ectomycorrhizas were examined. The mycorrhizal samples were collected at three progressional points in the natural hypogeous colony(shiro) : colony front edge, near the fruiting point and 20cm back. The fine roots in the colonies were typical ectomycorrhizas with fungal mantle and Hartig net. However, the T. matsutake mycorrhizas had unique characteristics compared to other types of ectomycorrhizas. That is, spatially the fungal mantle and Hartig net of the T. matsutake mycorrhizas continued to develop along the growing tip, while temporally those structures declined to shrink changing to black brown in the older part of the roots behind the actively growing tip portion. However, there was no mark that the fungal hyphae penetrated into either the cortical cells, endodermal cell layers or stele. The apical tips of the blackened roots remained alive to form new mycorrhizas with other fungi later. Therefore, we conclude that the mycorrhiza of T. matsutake+P. densiflora is rather a dynamic symbiosis that changes its position spatiotemporally as the root grows than either a simple parasitism or symbiosis.

• Journal of Korean Society of Forest Science
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• v.82 no.4
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• pp.366-380
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• 1993

Quercus acutissima ectomycorrhizae were classified as apical type, linear type, clavate type, diffuse type, pyramidal type, coralloid type, and nodular type. The surface texture of the fungal mantle at the initial stage of mycorrhizal formation was velvety. The surface texture of Pisolithus tinctorius(Pt) mycorrhizae at 30 September was well-developed felty mantle, Yellowish white. Except Pt all mycorrhizae formed by ectomycorrhizal fungi used to experiment were white with felty mantle. Mycorrhizae at the initial stage of mycorrhizae formation were creamy or creamy brown and swelled with thin mantle. Transverse and longitudinal sections showed radially-elongated cortical cell layers and epidermal cell with Hartig net. The transversal wideth of radially-elongated cortical and epidermal cells in the mycorrhizae with thick mantle on 30 September did not different with the mycorrhizae with thin mantle on the initial stage. Pt #250 formed coralloid mycorrizae but Pt KJ-1 did not although they are same species. On the mean length of linear type ectomycorrhizae of Pt KJ-1(2.21mm) was 1.5 times longer than that of Pt #250(1.32mm). The total dry weight of seedlings inoculated with Pt KJ-1, Pt #250, Lycoperdon pedicellatum, Scleroderma verrucosum were significantly heavier than those of suillus granulatus, Laccaria laccata.

• The Korean Journal of Mycology
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• v.37 no.1
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• pp.111-113
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• 2009

Ramaria botrytis is one of the commercially important ectomycorrhizal mushrooms in Korea and the mushroom is only picked in natural forest. This study was conducted to make ectomycorrhizal synthesis of the fungus using Pinus densiflora seedling. Ectomycorrhizal types of the fungus were simple and dichotomous. Diameter of ectomycorrhizal root was $325-380{\mu}m$ and fungal mantle was subhyaline with $20-30{\mu}m$ in diameter. On the other hand, optimum temperature for mycelial growth was $25^{\circ}C$ and the fungus grew 5 cm on potato dextrose agar medium in two months.