• Plant Resources
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• 제2권1호
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• pp.31-41
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• 1999

Many higher fungi were collected at Korea from 1976 to 1998. They were identified and surveyed on resources with many reference books. According to the results, fungal fungi were 40 families, 90 genera and 215 species. Among them, anti-cancer resources used in Korea were Ganoderma lucidum, Phellinus linteus, Agaricus brazei and Cordyceps militaris. Three species exception Agaricus brazei were distributed in Korea. All these are cultivated in Korea.

• 한국균학회지
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• 제49권1호
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• pp.1-10
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• 2021

A survey of fungal diversity in Gyeongsang Province, Korea, revealed two previously unreported Aspergillus isolates, named KMG411 and KMG412. The phylogeny of the isolates was analyzed based on β-tubulin (BenA) and calmodulin (CaM) sequencing. Morphological analyses further identified the KMG411 and KMG412 as A. insuetus and A. nomius, respectively. Here we provide detailed morphological descriptions of the previously unrecorded Aspergillus species.

• Mycobiology
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• 제45권3호
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• pp.150-159
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• 2017

This study analyzed the distribution of endophytic fungi in 3 coastal environments with different climatic, geographical, and geological characteristics: the volcanic islands of Dokdo, the East Sea, and the West Sea of Korea. The isolated fungal endophytes were characterized and analyzed with respect to the characteristics of their host environments. For this purpose, we selected common native coastal halophyte communities from three regions. Molecular identification of the fungal endophytes showed clear differences among the sampling sites and halophyte host species. Isolates were also characterized by growth at specific salinities or pH gradients, with reference to previous geographical, geological, and climate studies. Unlike the East Sea or West Sea isolates, some Dokdo Islands isolates showed endurable traits with growth in high salinity, and many showed growth under extremely alkaline conditions. A smaller proportion of West Sea coast isolates tolerate compared to the East Sea or Dokdo Islands isolates. These results suggest that these unique fungal biota developed through a close interaction between the host halophyte and their environment, even within the same halophyte species. Therefore, this study proposes the application of specific fungal resources for restoring sand dunes and salt-damaged agricultural lands and industrialization of halophytic plants.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 추계학술대회 및 정기총회
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• pp.47-47
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• 2014

Dokdo island is located in the northeastern part of Ulleungdo, known as volcanic island. In total, 53 fungal isolates were isolated from Dokdo island soil sample, using dilution plate technique. The isolates were identified on the basis of morphological characteristics and rDNA ITS sequence analysis. Out of them, 41 isolates were identified at the level of species. The dominant fungal species and genera included Fusarium spp., Mucor sp., Clonostachys spp., and Trichoderma sp. The % sequence identity (the number of matches/the complete alignment length) values via NCBI BLAST searching of EML-IF9, EML-MF30-1 and EML-DDSF4 represented 97.19% (485/499) with Clonostachys cf. rosea (GenBank accession no. KC313107), 98.33% (472/480) with Metarhizium guizhouense (GenBank accession no. HM055445), and 100% (350/350) with Mortierella oligospora (GenBank accession no. JX976032), respectively. Three species of C. rosea, M. guizhouense and M. oligospora represented new records of fungi from Dokdo island, Korea. The antimicrobial activities of the fungal strains varied with tested. Two isolates (EML-MFS30-1 and EML-IF9) showed antifungal activity against several fungi including Fusarium oxysporum and Rhizotonia solani. Clonostachys rosea (EML-IF9) showed strong hydrolytic enzyme activity. Our results showed that the antagonistic fungi including Clonostachys rosea will be used as potential biocontrol agents for control of fungal diseases.

• Mycobiology
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• 제47권1호
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• pp.50-58
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• 2019

Agarum clathratum, a brown macroalgae species, has recently become a serious environmental problem on the coasts of Korea. In an effort to solve this problem, fungal diversity associated with decaying A. clathratum was investigated and related ${\beta}$-glucosidase and endoglucanase activities were described. A total of 233 fungal strains were isolated from A. clathratum at 15 sites and identified 89 species based on morphology and a multigene analysis using the internal transcribed spacer region (ITS) and protein-coding genes including actin (act), ${\beta}$-tubulin (benA), calmodulin (CaM), and translation elongation factor (tef1). Acremonium, Corollospora, and Penicillium were the dominant genera, and Acremonium fuci and Corollospora gracilis were the dominant species. Fifty-one species exhibited cellulase activity, with A. fuci, Alfaria terrestris, Hypoxylon perforatum, P. madriti, and Pleosporales sp. Five showing the highest enzyme activities. Further enzyme quantification confirmed that these species had higher cellulase activity than P. crysogenum, a fungal species described in previous studies. This study lays the groundwork for bioremediation using fungi to remove decaying seaweed from populated areas and provides important background for potential industrial applications of environmentally friendly processes.

• 한국균학회지
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• 제43권1호
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• pp.13-19
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• 2015

낙동강 지류의 하천에 자생하는 5종류의 수생식물인 마름, 물억새, 미나리, 부들, 및 줄을 채집하였다. 수생식물의 뿌리에서 분리된 34균주의 내생균류는 ITS영역 염기 서열을 분석하였다. 분리된 모든 내생균류들에 대하여 다양성 지수와 계통분석을 수행하였다. 내생균류는 17속으로서, Acremonium속, Alternaria속, Aspergillus속, Cladosporium속, Emericellopsis속, Fusarium속, Galactomyces속, Leptosphaeria속, Microsphaeropsis속, Penicillium속, Peyronellaea속, Phoma속, Pseudeurotium속, Rhizomucor속, Talaromyces속, Trematosphaeria속, Zalerion속 등이 분리되었다. 5종류의 수생식물에서 Alternaria, Talaromyces속의 균류들이 비교적 많이 분포하고 있었다. 본 연구는 낙동강 지류의 하천 습지에 자생하는 수생식물 군락에서 공생하는 내생균류의 다양성에 대한 연구이다.

• 한국버섯학회지
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• 제17권4호
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• pp.179-184
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• 2019

본 연구는 표고 재배방식의 변화와 함께 톱밥재배가 증가됨에 따라 재배과정에서 발생 가능한 다양한 진균류를분리, 동정하고 이들에 대한 다양성 분석하고자 시험을 수행하였다. 경남지역 5개 농가로부터 수집된 시료에서 405균주를 분리하고 이들에 ITS 염기서열 분석을 통해 24속 42종을 확인하였다. 분리된 진균들에 대한 동정결과 Penicillium 속이 11종, Trichoderma속 4종이 확인되어 높은 다양성을 보여주었다. T. harzianum 의 경우 5개 농가에서 수집된 모든 시료에서 확인되었으며, Penicillium brevicompactum의 경우 3개 농가에 확인되었다. 2개 농가에서 확인되어진 Scytalidium cuboideum의 경우 표고 톱밥배지 조기부후 및 버섯 발생 저해 등의 피해증상이 확인됨에 따라 주의가 필요할 것으로 사료되었다. 또한 표고 톱밥재배의 경우 다양한 진균류이 존재가능하기 때문에 재배사 내부의 환경관리 및 예방이 필요할 것으로 판단되었다.

• 한국균학회지
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• 제48권4호
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• pp.457-473
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• 2020

본 연구에서는 국내 기수 환경의 물과 토양에서 균류의 다양성을 확인하기 위해 을숙도, 금강하구둑, 순천만습지, 대호방조제, 신두리사구 및 부남방조제 총 6지점의 현장조사 및 시료를 채집하였다. 현장에서 여과한 담수시료와 담수퇴적물로부터 곰팡이를 순수분리하여 PDA배지에 접종한 후 25℃에서 배양하였다. 기수역 시료에서 순수분리된 균주는 총 173균주로 ITS 부분을 분석하여 속 수준으로 동정하였다. 분리 동정된 균주를 다양성 지수를 이용하여 분석하였다. 분리된 균류는 Pleosporales, Eurotiales, Capnodiales, Hypocreales, Polyporales, Saccharomycetales, Agaricales, Glomerellales, Mucorales, Dothideales, Russulales, Xylariales, Sordariales, Myrmecridiales, Tubeufiales, Onygenales, Cantharellales, Amphisphaeriales 목에 속하며 Cladosporium속이 20%로 가장 우점하며, 그 다음은 Penicillium (19%), Fusarium (5%)의 순서로 많이 분포하였다. Sarocladium kiliense NNIBRFG3280와 Fusicolla merismoides NNIBRFG23708를 국내 기수환경에서 발굴하였으며, 이를 국내 미기록종으로 보고하고자 한다.

• Journal of Microbiology and Biotechnology
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• 제19권8호
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• pp.743-748
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• 2009

Fungal diversity during composting was investigated by culture-independent rDNA sequence analysis. Composting was carried out with pig manure and mushroom cultural waste using a field-scale composter (Hazaka system), and samples were collected at various stages. Based on partial sequence analysis of large subunit (LSU) ribosomal RNA (rRNA) and sequence identity values, a total of 12 different fungal species were found at six sampling sites; Geotrichum sp., Debaryomyces hansenii, Monographella nivalis, Acremonium strictum, Acremonium alternatum, Cladosporium sphaerospermum, Myriangium durosai, Pleurotus eryngii, Malassezia globosa, Malassezia restricta, Rhodotorula glutinis, and Fusarium sporotrichioides. Geotrichum sp. of the class Saccharomycetes was the most predominant fungal species throughout the composting process (185 out of a total of 236 identified clones, or 78.4%), followed by Acremonium strictum (7.6%), Monographella nivalis (5.1%), and Pleurotus eryngii (3.8%). The prevalence of Geotrichum sp. was the lowest (61.1%) at the beginning of composting, and then gradually increased to 92.5% after 10 days of composting.

• 한국산림과학회지
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• 제98권5호
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• pp.539-547
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• 2009

This study was conducted to investigate the arbuscular mycorrhizal fungal biodiversity, spore density and root colonization in relation to site ages at expressway cut-slopes in Korea. Stabilization of exposed surface involved soil amendments and spraying seed mixture of turf grasses and/or nitrogen-fixing shrub species. Eighteen sites were selected with varying ages (2 to 16 years). Soil samples collected in October from each site were analyzed for fungal diversity and spore counts. Fine root samples from the plants were assayed for fungal colonization. Of the total 37 plants inspected in the sites, 26 species had endomycorrhizal colonization with an average root colonization rate of 18%, and with a range from 1 to 67%. The average endomycorrhizal colonization rate of initially introduced Festuca arundinacea which became the most dominant grass in later stage showed 22.8%, while that of Lespedeza bicolor which became the most dominant woody species were 21.6%. Naturally-invading Robinia pseudoacacia showed higher colonization rate in the old sites. Although site age did not show significant effects on fungal diversity, the root colonization rates of initially introduced plants decreased with the site aging, while those of invading plants increased with aging of the sites. The soil chemical properties, pH, N, and P contents, were negatively correlated with spore density, root colonization and endomycorrhizal species diversity. A total of forty arbuscular mycorrhizal fungal species in seven genera were identified. Of the 40 species, Acaulospora lacunosa, Glomus aggregatum, Glomus constrictum, Scutellospora erythropa, and Acaulospora spinosa were the five most dominant species in the decreasing order.