• 한국식품저장유통학회지
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• 제10권4호
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• pp.498-505
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• 2003

만두피의 반죽제조에 키토산(시판 키토산 및 제조 키토산) 및 폴리인산, 구아껌, 타피오카 등의 성분 배합비율에 따른 반죽의 물성변화를 알아보고자 하였다. 그 결과는 다음과 같다. 제조된 균류키토산의 수분함량은 6.57∼6.58%, 질소함량은 6.71∼6.91%, 용해도는 99.05∼99.13%, 점도는 2.21∼2.23cps, 아세틸화도는 12.0∼l2.7% 그리고 분자량은 3.01${\times}$$10^{5}$∼3.12${\times}$$10^{5}$Dalton으로써 산처리 온도에 따른 FCs40과 FCs95의 성분 및 물리화학적 특성의 차이는 거의 없는 것으로 나타났다. 만두피 반죽에 첨가하는 폴리인산의 적절한 농도는 만두피 전체 성분 첨가량의 0.074%인 것으로 나타났으며, 폴리인산의 첨가량이 적거나 많이 함유하였을 때는 만두피 반죽의 견고성, 응집성, 탄력성 및 점착성 등이 오히려 감소하는 경향이었다. 타피오카의 첨가량에 따른 반죽의 물성에는 큰 영향을 주지는 않았으나, 첨가량은 전체성분 중량의 6.59%정도, 또한 구아껌의 경우는 0.062%정도가 만두피 반죽의 물성에 적절하였다. 만두피의 물성에 효과적인 키토산은 고분자량의 SCs, FCs40 및 FCs95 등으로 나타났다.타났다.

• 한국환경보건학회지
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• 제29권3호
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• pp.72-78
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• 2003

In the process of producing chitosan from crustacean shell, the use of excessive acid and alkli is causing the problems of environmental pollution and of production cost. In this study, one way to solve these problems is to cultivate fungi, then, to extract chitosan from the cell wall. By means of flask incubation and batch cultivation, the optimum cultivation conditions for mass production of continuous cultivation was found. Four strains used for the production of fungal chitosan were Gongronella butleri IF08080, Absidia coerulea IF05301, Rhizopus delemar IF04775, Mucor tuberculisporus IF09256. In flask incubation to select strain of producing much chitosan by means of experiment of the effect of initial pH, Absidia coerulea IFO 5301 had highest yield in FCs, 258.1 $\pm$ 47.3 mg/200 $m\ell$l at pH 6.5. In flask incubation under the optimum cultivation condition, temperature 27$^{\circ}C$, culture time 6days, glucose 2%, peptone 1%, (NH$_4$)$_2$ SO$_4$ 0.5%, $K_2$HPO$_4$ 0.1 %, Nacl 0.1 %, MgSO$_4$ㆍ7$H_2O$ 0.05%, CaCl$_2$ㆍ2$H_2O$ 0.01 %, the yield of DCW brought the highest yields. In batch bioreactor, the optimum cultivation condition was that cell suspended solution was 70 $m\ell$, aeration rate 0.5 l/min, agitation rate 800 rpm, culture time 36 hr. In continuous bioreactor, the optimum substrate flow rate was 4 ι/day.

• 한국환경보건학회:학술대회논문집
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• 한국환경보건학회 2003년도 Challenges and Achievements in Environmental Health
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• pp.116-122
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• 2003

The physicochemical properties of fungal chitosan at 95$^{\circ}C$ and 40$^{\circ}C$ acid treatment was as follows respectively. The nitrogen content was 6.71%, 6.91%, the viscosity 2.23cps, 2.21cps, the acetylation 12.0%, 12.7% and the molecular weight 3.12${\times}$10$\^$5/ Dalton, 3.01${\times}$10$\^$5/ Dalton. The absorbency band of reference, FCs-40 and FCs-95 in I.R. spectra was almost in accord with one another. In solid state NMR spectra, methyl group(-CH$_3$) was observed lightly. That means which deacetylation was well occurred. Carbonyl group(C=O) was not observed. C$_1$ to C$\_$6/ in solid state NMR was well observed seperately enough.