• Journal of the Korean Geographical Society
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• v.37 no.1
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• pp.15-40
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• 2002

This study is an attempt to grasp the spatial patterns and characteristics in the freight flows based on freight quantity data in 1997. The spatial background for the study is the Capital Region, which plays an important role in regional economics and industrial development. The methodology in the research used factor analysis to produce a functional region. The study selected three categories of freight that account for more than 60 percent of freight flows, chemical industrial goods, light industrial goods, and metal machinery industrial goods. The target analysis unit is divided into 36 zones. The common characteristic of the three categories is that they are concentrated at one specific region than are dispersed regionally. And in particular, the remarkable feature is that the central region of the study is Seoul. Usually the eminent regions of nows were made where environment of distribution facilities is good but these were made in a less than good environment. I think a reason is that facilities were uneven distribution.

• BMB Reports
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• v.35 no.2
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• pp.143-154
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• 2002

The structural and functional aspects of ervatamin B were studied in solution. Ervatamin B belongs to the $\alpha+\beta$ class of proteins. The intrinsic fluorescence emission maximum of the enzyme was at 350 nm under neutral conditions, and at 355 nm under denaturing conditions. Between pH 1.0-2.5 the enzyme exists in a partially unfolded state with minimum or no tertiary structure, and no proteolytic activity. At still lower pH, the enzyme regains substantial secondary structure, which is predominantly $\beta$-sheet conformation and shows a strong binding to 8-anilino-1-napthalene-sulfonic acid (ANS). In the presence of salt, the enzyme attains a similar state directly from the native state. Under neutral conditions, the enzyme was stable in urea, while the guanidine hydrochloride (GuHCl) induced equilibrium unfolding was cooperative. The GuHCl induced unfolding transition curves at pH 3.0 and 4.0 were non-coincidental, indicating the presence of intermediates in the unfolding pathway. This was substantiated by strong ANS binding that was observed at low concentrations of GuHCl at both pH 3.0 and 4.0. The urea induced transition curves at pH 3.0 were, however, coincidental, but non-cooperative. This indicates that the different structural units of the enzyme unfold in steps through intermediates. This observation is further supported by two emission maxima in ANS binding assay during urea denaturation. Hence, denaturant induced equilibrium unfolding pathway of ervatamin B, which differs from the acid induced unfolding pathway, is not a simple two-state transition but involves intermediates which probably accumulate at different stages of protein folding and hence adds a new dimension to the unfolding pathway of plant proteases of the papain superfamily.

• Proceedings of the SCSK Conference
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• 2003.09b
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• pp.508-518
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• 2003

SZ95 cell is an immortalized human sebaceous gland cell line that shows the morphologic, phenotypic and functional characteristics of normal human sebocytes. Sebocytes may play crucial parts in the pathophysiologic processes and disorders of the pilosebaceous unit. The secretory activity of the sebaceous gland is remarkably species-specific and acne is an exclusively human disease. Thus, this SZ95 cells offer possibilities for investigations on the physiology of the sebaceous gland and its role in sebum-associated skin disease such as acne. In this study, we investigated the effects of 13-cis-retinoic acid (13-cis-RA) and spironolactone, frequently used as therapeutic agents of acne, on the lipid synthesis and proliferation of human sebocytes. Cell proliferation was determined by MTT assay and cytoplasmic lipid droplets was shown by Oil-red a staining. Total lipid levels were biochemically estimated by the sulfo-phospho-vanilline reagent. 13-cis-RA and spironolactone significantly inhibited proliferation and lipid levels in a dose-dependent manner. Combined treatment with testosterone and 13-cis-RA or spironolactone resulted in a lower total lipid levels than that with androgen alone. These observations indicate that 13-cis-RA and spironolactone are potent inhibitors of both cell proliferation and lipid synthesis in human sebocytes. We will provide experimental evidence that this human sebocyte cell line serves as an adequate tool for evaluating the anti-lipogenic activity of various compounds potentially useful for the bioactive cosmeceutical ingredients on acne skin, and studying the intracellular biochemical markers depending on the types of compounds from various sources.

• YAKHAK HOEJI
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• v.37 no.4
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• pp.397-407
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• 1993

The muscarinic antagonist l-[benzilic-4,4'-$^3H$]quinuclidinyl benzilate([$^3H$]QNB) bound to a single class of muscarinic receptor with high affinity in guinea pig ileal membranes. The $K_{D}$ and B$_{max}$ values for [$^3H$]QNB calculated from analysis of saturation isotherms were 54 pM and 156fmol/mg, respectively. H$_{1}$-blockers inhibited [$^3H$]QNB binding to ileal membranes with $K_{i}$ values ranged from 0.008 $\mu{M}$ to 1.6 $\mu{M}$. The pseudo-Hill coefficients of H$_{1}$-blockers for inhibition of [$^3H$]QNB binding to the ileal membranes were close to unit. The $K_{i}$ values for H$_{1}$-blockers were similar to the $K_{M}$ values calculated by Schild plot of functional data obtained from inhibition of the carbachol-induced contraction in guinea-pig ileum, suggesting that binding of H$_{1}$-blockers vs [$^3H$]QNB in ileal membranes represents an interaction with a receptor of physiological relevance. The $K_{H}$ values of H$_{1}$-blockers for H$_{1}$-receptor estimated from inhibition of the histamine-induced contraction were the range of 0.15 nM to 56.5 nM. The $K_{M}$/K$_{H}$ ratio of H$_{1}$-blockers varied over a wide range of 3 to 2300. Thus, the antihistaminic potencies of H$_{1}$-blockers do not correlate with their antimuscarinic potencies, which suggest that antihistamines have different antimuscarinic potencies in therapeutic blood levels causing similar antiallergic effect. Among 13 traditional antihistaminics examined in this study, drug having the highest and the lowest $K_{M}$/K$_{H}$ ratio is triprolidine and diphenidol, respectively. The present results demonstrate that the antimuscarinic property of antihistamines is not necessary for their antiallergic effect, and data on the affinity of antihistamines for muscarinic and H$_{1}$-receptors can be an important parameter in the selection and evaluation of these drugs.

• Korean Institute of Interior Design Journal
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• v.21 no.3
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• pp.128-135
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• 2012

The purpose of this study is to investigate and analyze the compositional elements of plan of art museums by selecting architectural works, which have strong symbolic meaning when evaluating value, from architectural works by Louis Kahn. First, the centrality of artworks, which are being displayed at the Yale Art Gallery, is complex. It includes three patterns of Void (spatial) Centrality+Symbolic Centrality+Functional Centrality among the five patterns suggested in this study. Second, the ratio system of interior space, which is expressed in the extension of the Yale Art Gallery, can be classified into the floor and walls. The floor used the square 1:1 ratio system. The ceiling used the grid patterns of equilateral triangles (regular tetrahedrons). It was applying a geometric ratio system when creating forms by using one side as a beam and the other sides as decorations of the ceiling. Third, the contours of interior space, which were expressed in Kahn's works, used the method of forming separate space according to the contouring rule (structural unit) defined by columns, and they were used in constructing the entire space through the integration of separate spaces. Fourth, according to the characteristics that were expressed by artworks displayed within the Yale Art Gallery Extension, the concept of accessibility was not as clear as that of residential buildings, because an approach of respecting existing architecture and context was used, rather than the starting point of interior space, or differentiated circulation, which has the function of a determined position.

• The Transactions of the Korea Information Processing Society
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• v.5 no.3
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• pp.662-670
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• 1998

In order to overcome the problems with first generation expert systems at the symbol level, recently various knowledge level development techniques of second generation expert systems have been proposed. But, these techniques are conceptual modelling techniques. This paper modifies and complements these conceptual modelling techniques and proposes a Task Object Modelling (TOM) technique as a practical knowledge level expert system development technique. This paper defines a Task Object(TO) as a knowledge unit consisted of a goal, execution conditions, behaviour knowledge, and so on. And, we define a Task Object Diagram(TOD) to depict structual, dynamic, and functional aspects of TO easily. We also define Inference Types as basic units to describe behaviour knowledge of TOs. In order to utilize the proposed TOM technique as not a simple conceptual modelling technique but a practical second generalion expert system development technique, we implement a TOD editor, a TO editor, and TO processing algorithm based on the state of TOs. Also we implement a Inference Types Library, in which each inference type is corresponded to an IRE(Jntelligent Rules Element) method, to transform the defined inference types into IRE methods automatically.

• Applied Microscopy
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• v.28 no.2
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• pp.193-205
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• 1998

The Urechis unicinctus sperm and spermatogenic cells prepared from the testis are investigated to identify $\alpha-tubulin$ of axoneme microtubules using mouse monoclonal $anti-\alpha-tubulin$ as the first Ab and Gold(10nm) conjugated goat anti-mouse IgG as the Ab marker. The Ag-Ab reaction analyzed excellently the localization of $\alpha-tubulin$ and the gold particles incorporated with the proximal and distal centrioles, manchette microtubules, and flagellum. The gold particles can be also observed in the spermatogenic cells while the cells are still in sperm ball which is composed of a somatic cell and spermatogenic cells. The sperm ball is the functional unit of sperm production in U unicinctus testis. The spermatids are developed from the spermatogenic cells in the sperm ball and released into the testis cavity through a cortical cytoplasmic opening. The spermatid architectures are similar with the mature sperm of the testis cavity in aspects of shape of discoid acrosome, degree of nuclear condensation and ring type of mitochondrion. However, the distal centriole connecting with the flagella can be observed from the mature sperm while the both proximal and distal centrioles reveal only in the spermatids. The proximal centriole is directly connected with nuclear outer membrane during the stage of nuclear condensation and oriented perpendicularly to the distal centriole whose axis coinciding with the longitudinal axis of the spermatozoon. There are indications that the distal centriole is intimately associated with the polymerization of the flagellum. The manchette microtubules appear during spermatid development but the mature sperm have round head and no conspicuous middle piece.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.1
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• pp.16-21
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• 2012

This study was carried out to investigate the suitability of colored barley in malting and brewing properties and the possibility of utilizing pigments in colored barley as functional components in malting and brewing products. Purple and blue barley grains contained anthocyanins. However, about 80% and 20% of anthocyanins in the purple and blue barley grains, respectively, were lost during the steeping process. In malts, only 0.4~4.2% and 58.3% of anthocyanin in purple and blue barley grains, respectively, were remained. Wort color value was not affected by lemma color of black barley. In wort made from black barley, the color value was higher as its soluble nitrogen content higher. Anthocyanins were not found in wort and beer brewed from malts of purple and blue barley. The color value (EBC unit) was higher in wort and beer made from malts of purple and blue barley than those made from malt of the control variety, Hopum.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2003.11a
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• pp.533-538
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• 2003

It has been shown that ion implantation produces remarkable improvements in surface-sensitive physical and chemical properties as well as other mechanical properties, in polymers. In this study, ion implantation was performed onto polymer, PC(polycarbonate), in order to investigate surface hydrophilic property through contact angle measurement using distilled water. PC was irradiated with N, Ar, Xe ions at the irradiation energy of $20\;{\sim}\;50keV$ and the dose range of $5{\times}10^{15},\;1{\times}10^{16},\;7{\times}10^{16}\;ions/cm^2$. The contact angle of water has been reduced with increasing fluence and ion mass but increased with increasing implanted energy. The changes of chemical and structural property are discussed in view of infrared spectroscopy and FT-IR, XPS, which shows increasing C-O bonding and C-C bonding. The root mean square of surface roughness examined by means of AFM changed smoothly from 0.387nm to 0.207nm and the change of wettability was discussed with respect to elastic and inelastic collisions obtained as results of TRIM simulation. It was found that wettability of the modified PC surface was affected on change of functional group and nuclear stopping or linear energy transfer(LET, energy deposited per unit track length per ion) that causes chain scission by displacing atom from polymer chains, but was not greatly dependant on surface morphology.

• Proceedings of the KIEE Conference
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• 2004.11b
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• pp.190-193
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• 2004

Optimal Power Flow (OPF) is becoming more and more important in the deregulation environment of power pool and there is an urgent need of faster solution technique for on-line application. So this paper presents parallel genetic algorithm-tap search for the solution of the OPF. The control variables modeled unit active power outputs, generator-bus voltage magnitudes and transformer-tap settings. A number of functional operating constraints, such as branch flow limits, load bus boltage magnitude limits and generator reactive capabilities are included as penalties in the fitness function. In parallel GA-TS, GA operators are executed for each process. If best fitness of the GA is not changed for several generations, TS operators are executed for the upper three populations to enhance the local searching capabilities. With migration operation, best string of each node is transferred to the neighboring node after predetermined iterations are executed. For parallel computing, we developed a PC-cluster system consisting of 8 PCs. Each PC employs the 2 GHz Pentium IV CPU and is connected with others through ethernet switch based fast ethernet. To show the usefulness of the proposed method, developed algorithm has been tested and compared on an IEEE 30-bus system in the reference paper. From the simulation results, we can find that the proposed algorithm is efficient for the OPF.