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Adiponectin Gene Cloning and Its Expression in Insect Cell Expression System

  • Yuh, In Suh;Sheffield, Lewis G.
    • Reproductive and Developmental Biology
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    • v.36 no.3
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    • pp.193-198
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    • 2012

  • This study was to examine expression of the recombinant full-length adiponectin (recombinant adiponectin) in insect ovarian cell culture system and to characterize structural properties of the recombinant adiponectin secreted in medium. Gene construct encoding the recombinant adiponectin contained N-terminal collagen-like domain (110 Amino Acids, AAs), C-terminal globular domain (137 AAs) and C-terminal peptides for detection with V5 antibody (26 AAs included adaptor peptide) and purification using the 6xHis tag (6 AAs). The approximate molecular weight of the product (monomer) was 35 kDa. Molecular mass species of the expressed recombinant adiponectin were monomer (~35 kDa), dimer (~70 kDa), trimer (~105 kDa) and hexamer (~210 kDa). The major secreted species were the LMW forms, such as monomer, dimer, and trimer. There was MMW of hexamer as minor form. HMW multimers (~300 kDa) were shown as a tracer or not detected on the SDS-PAGE in several experiments (data not shown). The multimer forms in this study were not compatible to those in animal or human serum and adipose tissue by other researcher's study in which the major multimer forms were HMW. By protein denaturing experiments with reducing reagent (${\beta}$-MeOH), anionic detergent (SDS) and heat ($95^{\circ}C$) on the SDS-PAGE, not all adiponectin multimers seemed to have disulfide bond linked structure to form multimers. The recombinant adiponectin which expressed in insect ovarian cell culture system seemed to have the limitation as full physiological regulator for the application to animal and human study.

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Effects of Microwave Treatment on the Preservation of Foods (가정용 전자렌지의 마이크로파 처리가 식품의 보존성에 미치는 영향)

  • 우임선;고용덕
    • Food Science and Preservation
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    • v.4 no.1
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    • pp.17-25
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    • 1997

  • The effects of microwave treatment on the perservation of foods, such as a seaweed soup and sea stoned radish shreds, were studied. Microwave treatment of microbial cell suspensions revealed that viable cells decreased dramatically when heated to 6$0^{\circ}C$. However, it was unlikely that microwave treatment to 60 is enough to decrease the viable cell counts efficiently in a seaweed soup and radish shreds. It was thought that microwave heating to at least 7$0^{\circ}C$ as a final temperature was an important factor to reduce microbial cell counts in foods. When foods were heated to 7$0^{\circ}C$ with a repetitive 15 sec "on" followed by 30 sec "off", no big differences were observed in viable counts during storage at 2$0^{\circ}C$ for 3 days, as compared to those treated with a full power. The microwave treatment with three stages was designed to solve problems associated with variations depending on food volumes and difficulties of heat diffusion in a solid food to be irradiated with a microwave oven. The three stage method was found to have a similar efficiency in the reduction of viable cell counts in foods to microwave treatment at a full power and to conventional methods, such as water bath heating or boiling for 3 min with a gas range.in with a gas range.

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Cross section generation for a conceptual horizontal, compact high temperature gas reactor

  • Junsu Kang;Volkan Seker;Andrew Ward;Daniel Jabaay;Brendan Kochunas;Thomas Downar
    • Nuclear Engineering and Technology
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    • v.56 no.3
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    • pp.933-940
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    • 2024

  • A macroscopic cross section generation model was developed for the conceptual horizontal, compact high temperature gas reactor (HC-HTGR). Because there are many sources of spectral effects in the design and analysis of the core, conventional LWR methods have limitations for accurate simulation of the HC-HTGR using a neutron diffusion core neutronics simulator. Several super-cell model configurations were investigated to consider the spectral effect of neighboring cells. A new history variable was introduced for the existing library format to more accurately account for the history effect from neighboring nodes and reactivity control drums. The macroscopic cross section library was validated through comparison with cross sections generated using full core Monte Carlo models and single cell cross section for both 3D core steady-state problems and 2D and 3D depletion problems. Core calculations were then performed with the AGREE HTR neutronics and thermal-fluid core simulator using super-cell cross sections. With the new history variable, the super-cell cross sections were in good agreement with the full core cross sections even for problems with significant spectrum change during fuel shuffling and depletion.

  • https://doi.org/10.1016/j.net.2023.11.010 인용 PDF

Anatomical Structure and Fruit Quality According to the Fruit Developmental Stage as Affected by Gibberellins Treatments in Pyrus pyrifolia Nakai cv. Hanareum ('한아름'의 Gibberellin 처리에 따른 생육기별 해부학적 구조와 과실품질)

  • Park, Ji-Eun;Kwon, YongHee;Lee, ByulHaNa;Park, YoSup;Jung, Myung Hee;Choi, Jin-Ho;Park, Hee-Seung
    • Horticultural Science & Technology
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    • v.32 no.1
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    • pp.33-40
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    • 2014

  • This study was carried out to understand the physiological characteristics of early-matured 'Hanareum' (Pyrus pyrifolia Nakai) pears through anatomical structure and fruit characteristics and also the changes according to gibberellin (GA) treatment. The pericarp at full bloom consists of outer epidermis, hypodermis, parenchyma cell, and inner epidermis from the exterior and five types of vascular bundle tissues. Cork cell layer was formed at 70 days after full bloom (DAFB) in non-treated fruits and formed at 60 DAFB in GA treated fruits. Cell division period was from full bloom (FB) to 40 DAFB and then fruit enlargement was accomplished by the cell growth. Comparison of the fruit enlargement and fruit structure development by GA treatment or non-treatment showed that cell division of 'Hanaerum' fruits did not affect the GA treatment but fruit enlargement was affected cell growth. Fruit stalk of GA treatment fruits was larger than non-treated fruits from 40 DAFB which correspond to the period of the stop of cell division and 'Hanareum' was regarded GA treatment expedite of vascular bundle tissue growth and relatively increased nutrient transport to fruit. In addition to, average fruit quality between the non-treatment and GA treatment showed that fruit weight was higher in fruits treated by GA but firmness was lower and probably was effected fruit storing in 'Hanareum' pear.

  • https://doi.org/10.7235/hort.2014.13072 인용 PDF KSCI