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Transcriptome Profiling and In Silico Analysis of the Antimicrobial Peptides of the Grasshopper Oxya chinensis sinuosa

  • Kim, In-Woo;Markkandan, Kesavan;Lee, Joon Ha;Subramaniyam, Sathiyamoorthy;Yoo, Seungil;Park, Junhyung;Hwang, Jae Sam
    • Journal of Microbiology and Biotechnology
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    • v.26 no.11
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    • pp.1863-1870
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    • 2016
  • Antimicrobial peptides/proteins (AMPs) are present in all types of organisms, from microbes and plants to vertebrates and invertebrates such as insects. The grasshopper Oxya chinensis sinuosa is an insect species that is widely consumed around the world for its broad medicinal value. However, the lack of available genetic information for this species is an obstacle to understanding the full potential of its AMPs. Analysis of the O. chinensis sinuosa transcriptome and expression profile is essential for extending the available genetic information resources. In this study, we determined the whole-body transcriptome of O. chinensis sinuosa and analyzed the potential AMPs induced by bacterial immunization. A high-throughput RNA-Seq approach generated 94,348 contigs and 66,555 unigenes. Of these unigenes, 36,032 (54.14%) matched known proteins in the NCBI database in a BLAST search. Functional analysis demonstrated that 38,219 unigenes were clustered into 5,499 gene ontology terms. In addition, 26 cDNAs encoding novel AMPs were identified by an in silico approach using public databases. Our transcriptome dataset and AMP profile greatly improve our understanding of O. chinensis sinuosa genetics and provide a huge number of gene sequences for further study, including genes of known importance and genes of unknown function.

Texture Image Database Retrieval Using JPEG-2000 Partial Entropy Decoding (JPEG-2000 부분 엔트로피 복호화에 의향 질감 영상 데이터베이스 검색)

  • Park, Ha-Joong;Jung, Ho-Youl
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.32 no.5C
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    • pp.496-512
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    • 2007
  • In this paper, we propose a novel JPEG-2000 compressed image retrieval system using feature vector extracted through partial entropy decoding. Main idea of the proposed method is to utilize the context information that is generated during entropy encoding/decoding. In the framework of JPEG-2000, the context of a current coefficient is determined depending on the pattern of the significance and/or the sign of its neighbors in three bit-plane coding passes and four coding modes. The contexts provide a model for estimating the probability of each symbol to be coded. And they can efficiently describe texture images which have different pattern because they represent the local property of images. In addition, our system can directly search the images in the JPEG-2000 compressed domain without full decompression. Therefore, our proposed scheme can accelerate the work of retrieving images. We create various distortion and similarity image databases using MIT VisTex texture images for simulation. we evaluate the proposed algorithm comparing with the previous ones. Through simulations, we demonstrate that our method achieves good performance in terms of the retrieval accuracy as well as the computational complexity.

Real-Time Lane Detection Based on Inverse Perspective Transform and Search Range Prediction (역원근 변환과 검색 영역 예측에 의한 실시간 차선 인식)

  • Kim, S.H.;Lee, D.H.;Lee, M.H.;Be, J.I.
    • Proceedings of the KIEE Conference
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    • 2000.07d
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    • pp.2843-2845
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    • 2000
  • A lane detection based on a road model or feature all need correct acquirement of information on the lane in a image, It is inefficient to implement a lane detection algorithm through the full range of a image when being applied to a real road in real time because of the calculating time. This paper defines two searching range of detecting lane in a road, First is searching mode that is searching the lane without any prior information of a road, Second is recognition mode, which is able to reduce the size and change the position of a searching range by predicting the position of a lane through the acquired information in a previous frame. It is allow to extract accurately and efficiently the edge candidates points of a lane as not conducting an unnecessary searching. By means of removing the perspective effect of the edge candidate points which are acquired by using the inverse perspective transformation, we transform the edge candidate information in the Image Coordinate System(ICS) into the plane-view image in the World Coordinate System(WCS). We define linear approximation filter and remove the fault edge candidate points by using it This paper aims to approximate more correctly the lane of an actual road by applying the least-mean square method with the fault-removed edge information for curve fitting.

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Genomic Features of Retroelements and Implications for Human Disease

  • Kim, Heui-Soo
    • Genomics & Informatics
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    • v.3 no.4
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    • pp.133-141
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    • 2005
  • Most of the endogenous retroviral genes integrated into the primate genome after the split of New World monkeys in the Oligocene era, approximately 33 million years ago. Because they can change the structure of adjacent genes and move between and within chromosomes they may play important roles in evolutionas well as in many kinds of disease and the creation of genetic polymorphism. Comparative analysis of HERVs (human endogenous retroviruses) and their LTR (long terminal repeat) elements in the primate genomes will help us to understand the possible impact of HERV elements in the evolution and phylogeny of primates. For example, HERV-K LTR and SINE-R elements have been identified that have been subject to recent change in the course of primate evolution. They are specific elements to the human genome and could be related to biological function. The HERV-M element is related to the superfamily of HERV-K and is integrated into the periphilin gene as the truncated form, 5'LTR-gag-pol-3'LTR. PCR and RT-PCR approaches indicated that the insertion of various retrotransposable elements in a common ancestor genome may make different transcript variants in different primate species. Examination of the HERV-W elementrevealed that env fragments were detected on human chromosomes 1, 3-7, 12, 14, 17, 20, and X, whilst the pol fragments were detected on human chromosomes 2-8, 10-15, 20, 21, X, and Y. Bioinformatic blast search showed that almost full-length of the HERV-W family was identified on human chromosomes 1-8, 11-15, 17, 18, 21, and X. Expression analysis of HERV-W genes (gag, pol, and env) in human tissues by RT-PCR indicated that gag and pol were expressed in specific tissues, whilst env was constituitively expressed in all tissues examined. DNA sequence based phylogenetic analysis indicated that the gag, pol and env genes have evolved independently during primate evolution. It will thus be of considerable interest to expand the current HERV gene information of various primates and disease tissues.

Characteristics of Fashion Purchases and Clothes-wearing Tendencies of Women in their 30's Using Online Shopping (온라인 쇼핑을 활용하는 30대 여성의 패션상품 구매 및 착장의 특성)

  • Joo, Mi-Young;Kim, Young-In
    • Journal of the Korean Society of Costume
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    • v.64 no.8
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    • pp.1-19
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    • 2014
  • The purpose of this study is to do an in-depth examination of Korean women in their 30's on the characteristics of their online fashion shopping, clothes-wearing, and presentation. In-depth interview and quantitative analysis were conducted as study methods. Results of this in-depth analysis showed that the factor with the most significant influence in their lifestyle was "childbirth." Childbirth was a major factor during fashion shopping and clothes-wearing. Also the results showed that the reason they used online shopping was for convenience, efficiency, rationality, pursuit of information, variety, and hedonism. In particular, women in their 30's had a higher motivation for efficiency and rationality compared to those in their 20's, and of those women, married working women showed the highest preference for fashion soho malls. Meanwhile, full-time homemakers, who pursued rationality, used open markets to search for fashion items based on price. Furthermore, the factors that women in their 30's considered during online shopping were price, design, purpose or situation for wearing the clothing, respectively. Compared to the women in their 20's, they emphasized recommendation, product properties, credibility, economy more than women in their 20's. Factors such as marriage and childbirth were more influential than occupation. Meanwhile, the factors that women in their 30's considered for wearing and presentation were time, place, and occasion(TPO), which all showed high importance in in-depth interview and quantitative analysis. Other factors were 'suitable image to self' and 'covering up body figure.'

Verification of Automatic PAR Control System using DEVS Formalism (DEVS 형식론을 이용한 공항 PAR 관제 시스템 자동화 방안 검증)

  • Sung, Chang-ho;Koo, Jung;Kim, Tag-Gon;Kim, Ki-Hyung
    • Journal of the Korea Society for Simulation
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    • v.21 no.3
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    • pp.1-9
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    • 2012
  • This paper proposes automatic precision approach radar (PAR) control system using digital signal to increase the safety of aircraft, and discrete event systems specification (DEVS) methodology is utilized to verify the proposed system. Traditionally, a landing aircraft is controlled by the human voice of a final approach controller. However, the voice information can be missed during transmission, and pilots may also act improperly because of incorrectness of auditory signals. The proposed system enables the stable operation of the aircraft, regardless of the pilot's capability. Communicating DEVS (C-DEVS) is used to analyze and verify the behavior of the proposed system. A composed C-DEVS atomic model has overall composed discrete state sets of models, and the state sequence acquired through full state search is utilized to verify the safeness and the liveness of a system behavior. The C-DEVS model of the proposed system shows the same behavior with the traditional PAR control system.

A Study on the Analysis of the Mechanical Beauty of the Art Style Represented on the Second Half of the 20th Century Fashion(II) (20세기 후반 패션에 표현된 예술 양식의 기계미 분석 연구(II))

  • 이효진
    • The Research Journal of the Costume Culture
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    • v.6 no.3
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    • pp.126-156
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    • 1998
  • The purpose of this study was intended to analyze mechanical beauty of the art style represented on the second half of the 20th century fashion. From the early 20th century, when the mechanical civilization began to develop in full scale, various distinctive styles of art began to emerge by the denial of the traditional artistic style, which is said to be an inevitable consequence fo the 20th century's spirit. That is, the diversified styles of modern art, which has developed in line with the rapid growth of civilizations, experienced environmental pollution, non-humanization and un-individualization. Those phenomena became mental anguish for artists and designers, they have come to meet with changes, by their efforts to find new way. SO, the mechanical beauty is becoming a very important factor arts. Especially, the collage of the Cubism have used as art techniques of positive mechanical beauty. And in the transition from Dada to Surrealism, the objects provided an important harmony, suggesting that all things, even those achieved by chance or presented in new associations or radical dissociations, could be said to have meaning. Pop Art that expressed daily living of spending culture in the post industrial society, scaled up the induction of mass media which was much more realistic than Dada or Surrealism. According to the results of this study, the second half of the 20th century fashion has generated mechanical beauty was examined and plundered by modern art styles. The mechanical beauty of modern art was represented in the modern fashion as the positive and negative formativeness. Above all, the best way that the fashion design should take in the future, is in the search of ways how to restore humanity that was lost due to use of machine, how t develop its merits and how to harmonized with mechanical civilization.

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A Single Case Study of an Adolescent with a Mild Intellectual Disability in Preparation for Independence after Discharge from a Childcare Institution (아동양육시설 퇴소를 앞둔 경증지적장애 청소년의 자립준비를 위한 단일 사례연구)

  • Yu, Jin-Hee;Kim, Min-Kyeong
    • Journal of the Korea Convergence Society
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    • v.11 no.5
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    • pp.229-239
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    • 2020
  • The purpose of this study was to help young people with mild intellectual disabilities with counseling and self-supporting life experiences. The topic of the counseling contents could be categorized as follows: "institutional life was clumsy and hard, but it was also full of memories," "my adolescent was aching, growing, and lonesome," "an irresistibie being," "independence process is filled with anxiety, excitement." In his process of going independent, the subject showed a strong will for the preparation for independence where he gets through the unfavorable situation surrounding the arduous and lonely independent process. This result proves the effectiveness of the therapeutic intervention based on the coping skills that meet the needs of the social situation for the adolescents who are discharged from institutions in their independence preparation and job search.

A Fast Half Pixel Motion Estimation Method based on the Correlations between Integer pixel MVs and Half pixel MVs (정 화소 움직임 벡터와 반 화소 움직임 벡터의 상관성을 이용한 빠른 반 화소 움직임 추정 기법)

  • Yoon HyoSun;Lee GueeSang
    • The KIPS Transactions:PartB
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    • v.12B no.2 s.98
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    • pp.131-136
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    • 2005
  • Motion Estimation (ME) has been developed to remove redundant data contained in a sequence of image. And ME is an important part of video encoding systems, since it can significantly affect the qualify of an encoded sequences. Generally, ME consists of two stages, the integer pixel motion estimation and the half pixel motion estimation. Many methods have been developed to reduce the computational complexity at the integer pixel motion estimation. However, the studies are needed at the half pixel motion estimation to reduce the complexity. In this paper, a method based on the correlations between integer pixel motion vectors and half pixel motion vectors is proposed for the half pixel motion estimation. The proposed method has less computational complexity than the full half pixel search method (FHSM) that needs the bilinear interpolation of half pixels and examines nine half pixel points to the find the half pixel motion vector. Experimental results show that the speedup improvement of the proposed method over FHSM can be up to $2.5\~80$ times faster and the image quality degradation is about to $0.07\~0.69(dB)$.

Real-time Face Detection and Verification Method using PCA and LDA (PCA와 LDA를 이용한 실시간 얼굴 검출 및 검증 기법)

  • 홍은혜;고병철;변혜란
    • Journal of KIISE:Software and Applications
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    • v.31 no.2
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    • pp.213-223
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    • 2004
  • In this paper, we propose a new face detection method for real-time applications. It is based on the template-matching and appearance-based method. At first, we apply Min-max normalization with histogram equalization to the input image according to the variation of intensity. By applying the PCA transform to both the input image and template, PC components are obtained and they are applied to the LDA transform. Then, we estimate the distances between the input image and template, and we select one region which has the smallest distance. SVM is used for final decision whether the candidate face region is a real face or not. Since we detect a face region not the full region but within the $\pm$12 search window, our method shows a good speed and detection rate. Through the experiments with 6 category input videos, our algorithm shows the better performance than the existing methods that use only the PCA transform. and the PCA and LDA transform.