• The Korean Journal of Food And Nutrition
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• v.10 no.2
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• pp.213-218
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• 1997

Total lipid were extracted from the eggs, livers, intestines, muscles, and testis of Fugu xanthopterus. The fatty acid composition of total lipid was analyzed by gas liquid chromatography. Proximate percentage of total lipid from the samples was shown to be ; 81.72% in the livers, 14.53% in the intestines, 12.63% in the eggs, 0.85% in the muscles, and 1.74% in the testis. Toxins(tetrodotoxins) were completely removed from the liver of Fugu xanthopterus with 1% acetic acid/methanol solution before total lipid was extracted with CHCI3:methanol solution(2:1, v/v). The toxicity left in the total lipid was checked using mice. The content of DHA in each tissues of Fugu xanthopterus was the most abundant in muscle(32.4%), followed by liver(23.0%), intestine(26.7%) and testis(15.4%). The level of total polyenoic acids comprising DHA was more abundant in muscle(57.4%) than in the liver(50.9%).

• The Korean Journal of Food And Nutrition
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• v.10 no.2
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• pp.208-212
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• 1997

Total lipid were extracted from the eggs of Fugu xanthopterus and were then resolved into lipid subclasses by silica gel column chromatograpy. The fatty acid composition of total lipid, triacylglycerols, phospholipids and wax esters of these oils were analyzed by gas-liquid chromatography. Proximate percentage of total lipid from the sample was 12.63%. The lipid classes of eggs of Fugu xanthopterus fractionated by silica gel column chromatograpy were 59.37% of triacylglycerols, 15.46% of phospholipids, 6.9% of wax esters. The egg lipid obtained from Fugu xanthopterus was enriched with DHA in every class. In particular, the phospholipid contained the highest level of DHA(19.3%) than the other. The sum of polyenoic acids(27.4%). The fatty alcohols were mainly composed of saturated alcohols such as C16:0 alcohol(62.6%), C14:0(7.9%) and C18:0(5.8%), while the level of polyene alcohols was strikingly low compared with that of saturated alcohols.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.1
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• pp.46-52
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• 1996

The present study was undertaken to investigate the effect of the water extract of the puffer fish Fugu xanthopterus(FXH) on the alcohol induced hyperuricemia. The normal group and the FXH treated group showed no sigbificant changes in the levels of blood uric acid but, the blood uric acid significantly decreased in the FXh treated rats with 100mg/kg for two weeks compared to the ethanol treated group. There were no significant changes in the activities of uricase, adenosine deaminase, guanine deaminase, and purine uncleoside phosphorylase, among all the test group. But the activitis of liver xanthine oxidase were recovered to the normal level in ethanol +FXH treated group comparing to the ethanol treated group. Furthermore, ethanol+FXH treated rats showed the similar pattern in the levels of blood uric acid and urinary allantoin with normal group. These results indicate that the decreased blood uric acid by the FXH treatment of the alcohol induced hyperuricemia rats may result from decreased activity of hepatic xanthine oxidase.

• Journal of the Korean Society of Food Science and Nutrition
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• v.23 no.3
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• pp.502-508
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• 1994

A total of 24 specimens of the pufferfish, Fugu xanthopterus, purchased at a fixhmarket in Pusan, korea were examined for toxicity using the assay method of tetrodotoxin (TTX). Also, the toxins isolated from the puffer liver were partially purified and analyzed for their chemical composition by instrumental behaviors. On the whole, when the level of toxicity in each organ was analyzed compared to that of liver, they were 100 % of the lover, 92 % for the intestine, 75% for the skin, 17% for the muscle, 785 for the testis, 87% for the ovary, and 71% for bile. The highest and average scores of toxicity for the liver were 917 and $231{\pm}51MU/g$ liver, respectively. The toxins of the puffer gave four peaks in HPLC whose retention times (10, 20, 22 and 25 min) were close to those of TDA, TTX, 4-epi-TTX, and and -TTX, respectively.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.14 no.1
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• pp.15-36
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• 1978

Underwater sounds of some fishes and crabs were analyzed in the laboratory. The behavioral responses to the playback sounds of their feeding and croaking sound were investigated. The samples used in the experiment were as follows: Nibea albiflora, seriola quinqueradiata, Navodon modestus, Fugu xanthopterus, chrysophrys major, Scylla serrata, Telmessus acutidens, Charybdis japonica, and Portunus trituberculatus. The feeding and croaking sounds of the samples were recorded by a tape recorder through a hydrophone in an anechoic aquarium. The sound intensity level was measured by means of a sound level meter at an anechoic chamber. The frequency, intensity and wave form of various sounds were analyzed with an analyzing system consisting of a 1/3 octave filter set, a high speed level recorder, an amplifier, an octave band analyzer and an oscilloscope. The most successful recording was edited into a sequence of sound track which repeats sound emitting for 5 to 7 seconds after pausing for 5 to 7 seconds. The sequence was then reproduced into an anechoic aquarium through the under water speaker. The experimental anechoic aquarium used for the sample fishes was divided into the four sections with any three screens selected from 40$\times$40mm, 60$\times$60mm, 80$\times$80mm and 100$\times$100mm mushes according to the species of the fishes, besides that for crabs were not sectioned. The results of the investigation are as follows: 1. Of the feeding sound of fish, the frequency of wave from of the sound produced by Nibea albiflora and seriola quinqucradiata was 125～250Hz, that by Navodon modestus 63～125Hz, and that by Fugu xanthopterus 400～500Hz. The pressure level of the feeding sound produced by Nibea albiflora and Seriola quinqueradiata was 56～62db, that by Navodon modestus 57～59db, and that by Fugu xanthopterus 60～64db. 2. Of the croaking sound of Nibea albiflora, the frequency of the sound was 125～250Hz almost equivalent to that of feeding sound, and the pressure level was 62～63db, slightly higher than that of feeding sound. 3. Of the croaking sounds of crabs, the frequency of the sound produced by scylla serrata was 125～250Hz, that by Charybdis japonica and Telmessus acutidens 500～1,000Hz, and that by Portunus trituberculatus 250～500Hz. The pressure level of the croaking sound by Scylla serrata was 68～70db, and that by Charybdis japonica, Telmessus acutidens and Portuens trituberculatus 50～62db. 4. Phonotactic responses of Nibea albiflora and Seriola quinqueradiata to the feeding sounds produced by their own species, the same body length were conspicuous with the phonotactic index of 56～87%, but that of Navodon modestus, Chrysophrys major and Fugu xanthopterus were hardly recognized. 5. Phonotactic responses of the sample fishes to the sinusoidal sound with the frequency range of 50 to 9,000 Hz were observed not conspicuous. 6. Phonotactic responses of Portunus trituberculatus to the croaking sounds produced by their own species was varied in the range of 40～100%, according to the carapace length and the sex.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.24 no.6
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• pp.363-368
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• 1991

Twenty-four specimens of the pufferfish Fugu xantheptens, Korean name, 'Ggachibog', collected at a fish market of Pusan were examined for anatomical distribution of toxicity by the mouse bioassay method. Frequency of toxic specimens was 88, 75, 54, 13, 71, 80 and $71\%$, in terms of liver, intestine, skin, muscle, testis, ovary and bile, respectively. Their the highest toxicity scores were 417, 387, 112, 17, 39, 403 and 178 MU/g, respectively; and average toxicity values were $110\pm25.0(mean \pm S.E.)$, $73\pm20.3,\;17.8\pm 5.1,\;2.7\pm1.1,\;15.6\pm5.4,\;115\pm33.0\;and\;34\pm9.3 MU/g,$ respectively. A significant correlation between the toxicities of liver and intestine(r=0.93), between those of liver and skin (r=0.79) and between those of liver and ovary(r=0.83) was observed.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.27 no.6
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• pp.682-689
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• 1994

Ninety-six specimens of nine puffer fishes landed at a fish market of Pusan, Korea were assayed for anatomical distribution of toxicity. The puffers, Lagocephalus gloveri ('geommilbog'), Fugu rubripes rubripes ('jajubog'), Fugu rubripes chinesis ('geomjajubog'), Lagocephalus wheeleri ('hinmilbog') and Arothron firmamentum ('byeolbog'), were shown to be nontoxic in muscle and skin. However, toxicities in skins of four specimens of Lagocephalus gloveri and a specimen of Arothron firmamentum were more than 10MU/g In the puffer, Fugu xanthopterus ('ggachibog'), the skin showed to be weakly toxic as far as nine specimens out of 27 ones are concerned, and the muscle was nontoxic in all specimens. Meanwhile, it was noted that the puffer, Fugu vermicularis radiatus was found to be a toxic species, differing from the toxicity known so far. Their highest toxicities were 3,880, 1,191, 1,115, 219, 289, 5,620 and 753MU/g for liver, intestine, skin, muscle, testis, ovary and bile, respectively.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.24 no.4
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• pp.144-149
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• 1988

The purpose of this study is to find the light intensity which induced maximum gathering rate and to observe the variation of the gathering rate both in daytime and tat night by using Striped puffer, Fugu xanthopterus (Temminck et Schlegel). An experimental tank(360L$\times$50W$\times$55H cm) was set up in a dark room. An illumination system was attached to the end of one side of the tank to control horizontal light intensity. Eight artificial light sources were prepared by combination of three light bulbs (10W, 60W, 100W) and eight filters. During the experiment water depth was maintained 50 cm level in the tank. The tank was marked into six longitudinal sections each being 60cm long to observe the distribution of fish. The fish were acclimatized in dark condition for 50 minutes prior to the main experiment. Upon turning on the light, the number of fish in each section was counted 60 times every 30 seconds, and the gathering rate was obtained from the average number of fish in each section. The light intensity inducing maximum gathering rate was 298.56 lux (188.44-444.96 lux) at daytime and 298.56 lux (188.44-444.96 lux) at night. The variation of the gathering rate of fish in illumination time was increasing trend fluctuately and did not show any distinctive difference between day and night.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.30 no.2
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• pp.78-85
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• 1994

The author carried out an experiment to find out the response of Striped puffer. Fugu xanthoperus (Temminck et Schlegel) to the color lights. The experimental tank (300L$\times$50W$\times$50Hcm) was set up in a dark room. Six longitudinal sections with 60cm intervals are marked in the tank to observe the location of the fish. Water depth in the tank was kept 50cm level. Light bulbs of 20W at the both ends of the tank projected the light horizontally into the tank. Two different colored filters were selected from four colors of red, blue, yellow, and white, and the were placed in front of the light bulbs to make different colors of light. Light intensity was controlled by use of auxiliary filiters intercepted between the bulb and the filter. The fishes were acclimatized in the dark for 60 minutes before they were employed in the experiment. Upon turning on the light, the number of fish in each section was counted 40 times in 30 second intervals, and the mean of the number of fish in each section was counted 40 times in 30 second intervals, and the mean of the number of fish in each section was given as the gathering rate of the fish. The colors favourited by the fish was found in order of blue, yellow, white and red in the daytime, and blue, white, yellow and red at night. The difference of the average distribution on two different colors of light was 13.12%(4.10-26.55%), and the difference in the daytime(14.79%) was larger than at night (11.45%). Constantly the gathering rate of fish on illumination period was fluctuated with instability. As the gathering rate of fish on illumination period was fluctuated with instability. As the gathering rate on one color of light increased, the gathering rate on the other color of light decreased. The difference of the gathering rate on two different colors of light was comparatively distinct and the difference in the daytime was larger than at night.

• Korean journal of food and cookery science
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• v.6 no.2
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• pp.85-96
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• 1990

Globefish, Fugu xanthopterus, known to have a severe toxin, is one of the favorite food in Korea and Japan when the toxic part is removed. In this paper, the effect of cooking on the composition of nitrogenous components in the extractives from globefish cooked investigated and the changes of the taste compounds originated from the nitrogenous components in the extractives were discussed. When the sample fish was analysed for general composition, drip amount and pH by the different methods of thawing, the method effective method was the running water thawing. Total nitrogen content in raw globefish and the frozen globefish was not different, and the nitrogen content was increased with the heat treatment. It seemed that the nitrogen content was higher in the extract from the boiled globefish than that of the steamed globefish. Taurine, lysine, glycine and alanine were occupied about 70% of the total free amino acids. Total free amino acid content was higher in the extracts from the frozen sample than those from the raw sample. The amount of free amino acids was increased when the globefish soup cooked under the direct-heat cooking than in the microwave oven-heat cooking. Among nucleotides in the extracts from the thawed and cooked fishes, IMP and inosine contents were increased, and the both components were decreased with the heating time and by the heating method. Tne content of total creatinine-nitrogen were 50% of the total nitrogen content of the extracts, but the concentration of glycinebetaine, TMA and TMAO were only a few amounts. It could be concluded that total creatinine components, including free amino acids such as taurine, lysine, glycine and alanine, and IMP might be the important components contributing to the taste of the cooked globefish.