• 제목/요약/키워드: Fugu xanthoperus

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까치복(Fugu xanthopterus)의 독성 (Tetrodotoxin in a Pufferfish, Fugu xanthopterus (Korean Name, Ggachibog))

  • Hyun-Dae Kim;Yeung-Ho Park;Dong-Soo Kim
    • 한국식품영양과학회지
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    • 제23권3호
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    • pp.502-508
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    • 1994
  • 부산 공동어시장에서 구입된 까치복어, Fugu xanthopterus의 조직부위별 독력을 mouse bioassay법에 의하여 비교 검사하였다. 또한 bio gel P-2 column chromatography으로 복어독을 부분 정제하여, 박층 chromatography, 전기영동, GC-MS 및 HPLC에 의하여 독의 조성을 분석하였다. 즉, 까치복의 개체당 전체 독력은 1,099~167,694 M로서 개체에 따라 뚜렷한 독력 차이가 있었다. 간, 난소 그리고 내장의 평균독력은 각각 $231.0{\pm}51.0,\;175.0{\pm}38.0\;및\;78.8{\pm}16.8MU/g(평균값{\pm}표준오차)$으로 나타난 반면에 근육과 껍질조직의 평균독력은 각각 $3.3{\pm}1.4와\;19.3{\pm}4.3MU/g$으로 개체에 따라 약독 내지 무독인 것으로 나타났다. 또한 각부위별 최고독력의 순서는 간(917MU/g), 난소(459MU/g), 내장(312MU/g), 담즙(101MU/g), 껍질(79MU/g), 정소(72MU/g), 그리고 근육조직 (27MU/g)의 순이었다. 한편, TLC, 전기영동, HPLC로 분석한 결과 복어 개체에 따라 독의 조성이 다름을 알 수 있었고, TTX를 알칼리분해 시켜 생성된 ${C_9}-base$를 TMS화 시켜 GC-MS에 주입시킨 결과 ${C_9}-base$ TMS 유도체의 특성인 406(molecular ion peak), 392(base), 380 및 376 m/z에서 fragment ions에 의해 TTX의 peak는 동일한 mass spectra로 나타났다. 따라서, 이들의 독성에 관한 구체적인 연구자료는 최근들어 수입 복어류의 증가, 양식어업의 발달, 식생활의 다양화 등에 따른 수산 식품의 위생적 안정성을 확보하는데 절실히 필요한 것이라 생각된다.

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색광에 대한 까치복의 반응 (Response of Striped Puffer , Fugu xanthopterus to the Colored Lights)

  • 양용림
    • 수산해양기술연구
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    • 제30권2호
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    • pp.78-85
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    • 1994
  • The author carried out an experiment to find out the response of Striped puffer. Fugu xanthoperus (Temminck et Schlegel) to the color lights. The experimental tank (300L$\times$50W$\times$50Hcm) was set up in a dark room. Six longitudinal sections with 60cm intervals are marked in the tank to observe the location of the fish. Water depth in the tank was kept 50cm level. Light bulbs of 20W at the both ends of the tank projected the light horizontally into the tank. Two different colored filters were selected from four colors of red, blue, yellow, and white, and the were placed in front of the light bulbs to make different colors of light. Light intensity was controlled by use of auxiliary filiters intercepted between the bulb and the filter. The fishes were acclimatized in the dark for 60 minutes before they were employed in the experiment. Upon turning on the light, the number of fish in each section was counted 40 times in 30 second intervals, and the mean of the number of fish in each section was counted 40 times in 30 second intervals, and the mean of the number of fish in each section was given as the gathering rate of the fish. The colors favourited by the fish was found in order of blue, yellow, white and red in the daytime, and blue, white, yellow and red at night. The difference of the average distribution on two different colors of light was 13.12%(4.10-26.55%), and the difference in the daytime(14.79%) was larger than at night (11.45%). Constantly the gathering rate of fish on illumination period was fluctuated with instability. As the gathering rate of fish on illumination period was fluctuated with instability. As the gathering rate on one color of light increased, the gathering rate on the other color of light decreased. The difference of the gathering rate on two different colors of light was comparatively distinct and the difference in the daytime was larger than at night.

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