• Journal of Mushroom
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• v.18 no.4
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• pp.317-322
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• 2020

This experiment was conducted to solve the failure of fruiting body production in the bottle cultivation of the oyster mushroom cultivar 'Heuktari'. The effects of incubation temperature on primordium formation and fruiting body yield of the oyster mushroom cultivar 'Heuktari' were investigated. The proper temperature for mycelium growth of 'Heuktari' on potato dextrose agar (PDA) medium is 23-26℃. The mycelial growth of 'Heuktari' was faster than that of Chunchu 2ho. During mycelial culture in sawdust medium, the temperature of the medium in the bottle initially increased, reached the highest point in the middle of the culture, and then decreased. The higher the set temperature, the shorter the incubation period. When the incubation temperatures were 20℃ and 24℃, respectively, the undeveloped primordium formation rates were low (1.8% and 4.2%, respectively). However, the rate of undeveloped primordium formation increased, and the yield decreased at incubation temperatures of 16℃ and 28℃. Mushroom farms that set incubation temperatures to 18℃ and maintained the medium temperature at less than 28℃ showed undeveloped primordium formation rates ranging between 0.3-0.8%. The rate of undeveloped primordium formation increased and the yield decreased in the farms with high incubation temperatures (above 28℃). We found that in order to reduce undeveloped primordium formation, the air inside the incubation room should be circulated continuously so that the temperature of the medium does not rise above 28℃, and dense incubation conditions should be avoided.

• Journal of Mushroom
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• v.13 no.2
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• pp.145-150
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• 2015

Mushroom sciarid fly Lycoriella ingenua (Dufour), syn. L. mali (Fitch) is one of the most common fly pests affecting the mushroom cultivation in Korea. This study was carried out to investigate the development at different temperatures and damages of L. ingenua in A. bisporus mushroom cultivation. Rearing of mushroom flies were carried out on mycelial culture in Petri dishes. The development of L. ingenua from egg to adult at the temperature of 16, 20, 24, $28^{\circ}C$ were 35.2, 25.8, 23.5, and 22.2 days, respectively. Adult flies invade mushroom farms and oviposit in freshly spawned compost. Damages are mainly caused by the larvae of 1-7 mm, which feed on growing mycelium and also developing stipes and caps of mushrooms. Adult flies spoil the appearance of the fruiting bodies, spread various fungal and bacterial disease, and transmit mites. The damages by mushroom flies increased without pest control causing yield loss of up to 27%.

• Journal of Mushroom
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• v.13 no.3
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• pp.203-206
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• 2015

'Baekjung' adaptable to high temperature was made by crossing between monokaryon derived from selfing of brown strain and monokaryon derived from Korea white strain. In the condition that temperature is maintained at $10^{\circ}C$ without low temperature of $4^{\circ}C$ suppressing treatment and wrapping during cultivation period, it showed good productivity than Uri1ho(control). The optimum temperature of mycelial growth was $30^{\circ}C$ and that of fruiting body initiation and development were $14^{\circ}C$ and $7^{\circ}C$, respectively. The days for the fruiting and yield were 7 days and $277{\pm}11.2$ g per 1,100 ml bottle, respectively. This variety needed high concentration of carbon dioxide up to 4,000 ppm for the good quality.

• Journal of Mushroom
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• v.13 no.4
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• pp.330-333
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• 2015

This study was carried out to compare the anti-inflammation effects of various fruiting body of Ganoderma species and Cordyceps militaris, Phelinus linteus extracts. We concentrated Ganoderma species and other medicinal mushrooms by extracting with ethanol. And We made it $100{\mu}g/ml$ concentration. As a result of nitrite scavenging activity, in the contrast to the positive control; Ascorbic acid was 25%, ASI 7080 of Ganoderma species was disappeared up to around 40%. And in the contrast to Ascorbic acid was 55%, ASI 7002 was 78.5% that was the highest anti-inflammation effect in the result of "No assay test". The Cordyceps militaris showed 75% and Hericium erinaceus showed 59.7% of anti-inflammation effect. As a result of the fungus yield control test of $TNF-{\alpha}$ through ELISA method to ASI 7002 of Ganoderma species that showed the highest anti-inflammation, it was reduced as same as LPS non-treatment. We extracted RNA from ASI 7002 Ganoderma species 10, 50, $100{\mu}g/ml$ concentration and LPS $10{\mu}g/ml$ of Raw 264.7 cell. And we tested the expression of iNOS, COX-2 and TNF-a that are kinds of inflammation gene after synthesizing RNA with cDNA. Finally we could find that iNOS, COX-2 and TNF-a were all controlled expression in the result of above experiment.

• Journal of Mushroom
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• v.11 no.4
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• pp.249-253
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• 2013

'Gonji10ho', a new variety of Neolentinus lepideus, was bred by mating two monokaryotic strains isolated from 'GMNL66037' and 'GMNL66014' in Mushroom Research Institute, Gyonggi-Do ARES in 2012. The optimum temperature for the mycelial growth of 'Gonji10ho' was $29^{\circ}C$ on PDA medium and that for the primordia formation and the growth of fruiting body of 'Gonji10ho' was $20{\pm}1^{\circ}C$. In bottle cultivation of 'Gonji10ho', the period of spawn running was around 35 days at $22{\pm}1^{\circ}C$ and the period from scratching of inoculum to harvesting was 27 days. 'Gonji10ho' had thicker pileus and stipe than control variety('Solhyang'). The number of available stipe was fewer than that of control variety. During the cultivation of 'Conji10ho', the occurrence rate of Trichoderma spp. was lower than that in control variety. In bottle cultivation, the yield of fruiting bodies of 'Gonji10ho', which was 38% higher than that of control variety, was 69.6 g/900 ml bottle, and in bag cultivation, that of 'Gonji10ho', which was 10% higher than that of control variety, was 114.2 g/1 kg bag. In physical characteristics, the strongness and brittleness of the pileus of 'Gonji10ho' were higher than those of control variety. When considered in the period available for sale, the shelf life of 'Gonji10ho', which was 5 days longer than that of control variety, was 34 days.

• Korean Journal of Breeding Science
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• v.42 no.6
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• pp.711-716
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• 2010

'Sanggang', a new cultivar of Agrocybe aegerita, was bred with mating between monokaryotic strains isolated from 'GMAG45109' and 'GMAG45107' in Mushroom Research Station, Gyonggi Province A.R.E.S. in 2009. The optimum temperature for the mycelial growth of 'Sanggang' was 26 to 28$^{\circ}C$ on PDA(potato dextrose agar) medium. The optimum temperature for the primordia formation and fruiting body development of 'Sanggang', was 18 to 20$^{\circ}C$. In the bottle cultivation of 'Sanggang', the period of spawn running was around 38 days at 22 to 23$^{\circ}C$ and the period from scratching of inoculum to harvest was 12 days. These characteristics of 'Sanggang' were not different from those of 'Mihwang' (control). 'Sanggang' had brown-colored pileus, whereas 'Mihwang' had yellowish brown-colored one. In bottle cultivation, 'Sanggang' hada little smaller but stronger stipe and pilus than 'Mihwang', and 'Sanggang', as well as 'Mihwang', had upright stipe. And 'Sanggang' was shown to have lower veil opening ratio than that of 'Mihwang' at harvest time.The yield of fruiting bodies of 'Sanggang' was 134 g/850ml bottle, which was similar to that of 'Mihwang'. Resistance of'Sanggang' against Trichoderma spp., as well as 'Mihwang', was weak. Freshness of 'Sanggang' was maintained for 13 days under storage temperature 4$^{\circ}C$, while that of 'Mihwang' was maintained for 10 days.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.288-293
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• 2010

'Cham', a new cultivar of Agrocybe aegerita, was bred by crossing between monokaryotic strains isolated from GMAG45021 and 'Beodeulsongi1ho' in Mushroom Research Station, Gyonggi Province A.R.E.S. in 2007. The optimum temperature for the mycelial growth of 'Cham' was 26 to $28^{\circ}C$ on PDA medium, whereas that of 'Beodeulsongi1ho'(control) was 24 to $26^{\circ}C$. The optimum temperature for the primordial formation and fruiting body development of 'Cham', as well as 'Beodeulsongi1ho', was 18 to $20^{\circ}C$. In the bottle cultivation, the spawn run period of 'Cham' at 22 to $23^{\circ}C$ was around 38 days and the period from scratching of inoculum to harvest was 12 days. These characteristics of 'Cham' were not different from those of 'Beodeulsongi1ho'. However, 'Cham' had more dark brown-colored, and thicker and stronger pileus, and longer and thicker stipe compared to that of 'Beodeulsongilho'. Freshness of 'Cham' was maintained for 10 days at the storage temperature of $4^{\circ}C$, while that of 'Beodeulsongi1ho' was maintained for 8 days. Moreover, the yield of fruiting bodies of 'Cham' was $141g/850m{\ell}$ bottle, which was similar to that of 'Beodeulsongi1ho'. Both 'Cham' and 'Beodeulsongilho' have lower resistivity against Trichoderma virens and Trichoderma harzianum.

• Journal of Mushroom
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• v.20 no.3
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• pp.147-152
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• 2022

To develop mushroom varieties for cultivating at low temperature on oak logs, a strain with a low fruiting body generation temperature was crossed with Di-mon to select for a line with excellent properties. Selection was followed by cultivation testing. From these studies, Heunghwa 1ho was identified. The optimum temperature for cultivating Heunghwa 1ho, was 13.3℃. The fruiting temperature range was 6.4~20.2℃, identical to that of the parent strain. Growth at 25℃ for 7 days achieved optimal mycelial growth of 61.9±2.10 mm, superior to growth of the parent strain at this temperature. The cap shape of Heunghwa 1ho was convex, cap diameter was 57.8±8.31 mm, and cap color was brown. Heunghwa 1ho showed similar genetic traits to those of the parental strain. However, dry weight (20.1 kg/m³) and cap diameter and color are superior to those of the parent strain. The 3 year fresh oak mushroom yield was 113.8 kg/m³, superior to the respective yields of the parent strains JMI 10047 and JMI 90021 (92.5 kg/m³ and 66.4 kg/m³).

• Journal of Mushroom
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• v.13 no.1
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• pp.21-25
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• 2015

This study was carried out to develop optimum sawdust medium for bottle cultivation of Pholiota adiposa. Five kinds of sawdust media were tested. Period of primordial formation was shorter 1-5 days in T3 and in T4 than in other treatments. Total cultivation period was 3-5 days longer in treatments including oak sawdust than in T5, treatment of poplar+rice bran(8:2). Stipe diameter, stipe length, and number of valid stipe were the biggest in T3. Yield of fruiting body was increased 33% in T3 and 12% in T4, respectively compared to T1 as control. Therefore, T3, treatment of oak sawdust+beet pulf+cottonseed meal(5:3:2), would be appropriate for the commercial production of Pholiota adiposa by bottle cultivation.

• Journal of Mushroom
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• v.5 no.1
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• pp.21-24
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• 2007

A new variety of oyster mushroom 'Choengsan' was developed with crossing between monokaryotic strains derived from Suhan and Wonhyoung #3. It was developed by Agriproduct Processing Experiment Station, Gangwon Agricultural Research & Extension Services in 2006. The optimum temperature of mycelial growth was around $27{\sim}28^{\circ}C$ and that of fruiting body was $14{\sim}17^{\circ}C$. The color of pileus was dark-gray color. The period of primordial occurrence was about 20 days in bed cultivation using cotton waste and the yields were $38kg/3.3m^2$.