• 한국식품영양과학회지
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• 제38권10호
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• pp.1438-1443
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• 2009

쌀빵 냉동반죽에 첨가제로 gum, 유화제 및 효소제를 복합처리하여 반죽한 후 $-20^{\circ}C$에서 냉동 보관하면서 냉동반죽의 저장기간에 따른 쌀빵의 품질 특성을 조사하였다. 유화제인 sodium stearoyl lactylate(SSL)와 gum류인 guar gum을 각각 0.5%, 그리고 효소제로 곰팡이 $\alpha$-amylase를 0.01% 수준으로 첨가하여 사용하였다. 냉동반죽의 저장 중 쌀빵의 부피는 냉동기간이 경과함에 따라 감소하였는데, 첨가제를 넣지 않은 대조구 반죽에 비해 guar gum+SSL+곰팡이 $\alpha$-amylase를 복합첨가 한 반죽이 냉동 4주 경과 후에도 쌀빵 제조시 부피의 감소폭이 크지 않은 것으로 나타났다. 쌀빵의 경도 변화에 있어서 대조구 냉동반죽의 냉동저장 기간이 증가함에 따라 그리고 쌀빵의 제조 후 시간이 경과할수록 경도의 변화가 큰 반면, guar gum+SSL 첨가한 쌀빵과 guar gum+SSL+곰팡이 $\alpha$-amylase를 복합첨가 한 쌀빵은 저장기간에 따른 경도의 증가폭이 현저하게 낮은 것으로 나타났다. Gum/유화제/효소제의 복합첨가는 쌀가루 냉동반죽의 저장기간 경과에 따른 쌀빵의 부피 감소를 개선하고 노화를 억제하는 효과를 주어 냉동반죽 쌀빵의 품질에 긍정적인 영향을 미치는 것으로 평가되었다.

• 한국식품영양과학회지
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• 제41권2호
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• pp.239-245
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• 2012

여러 가지 약리활성 성분을 지니고 있는 양파 품종 중 자색양파의 효율적인 이용 방안으로 소비자의 기호에 맞는 자색양파설기의 개발을 위해 냉동보관 자색양파와 실온보관 자색양파의 첨가량을 달리하여 설기를 제조하고 자색양파설기의 최적 조건을 제시하기 위해 $25^{\circ}C$에서 저장하면서 색도 측정, pH 측정 및 기계적인 텍스쳐를 분석하였고, 아울러 소비자의 호에 맞는 자색양파설기의 맛과 질감을 알아보기 위해 관능평가도 수행하였다. 자색양파의 첨가량이 많을수록 명도 값은 실온보관 자색양파의 경우 유의적으로 감소하였으나, 냉동보관 자색양파의 경우 유의적으로 증가하였다. 저장기간에 따른 명도 값은 자색양파설기 제조당일부터 제조 2일 경과 후까지 유의적으로 감소하였지만 제조 3일 경과 후는 약간의 증가세를 보였다. 적색도(a값)는 자색양파를 첨가함으로써 유의적으로 높아졌으며 저장기간에 따라 전반적인 증가세를 보였지만 냉동 또는 실온 보관 자색양파 30%를 첨가한 설기는 유의적으로 감소함을 알 수 있었다. 황색도(b값)는 실온보관 자색양파의 첨가량이 늘수록 증가한 반면 냉동보관 자색양파의 첨가량이 늘면서 유의적인 감소세를 보였고 저장기간에 따라서는 실온 또는 냉동 보관 자색양파 첨가 설기 모두 유의적인 증가세를 나타내었다. 실온 및 냉동 보관 자색양파를 20% 첨가하여 제조한 설기의 pH가 가장 높았으며 2일 경과 후 증가, 3일 이후 다시 감소하는 경향은 전보의 팽창제 첨가에 따른 쑥설기 pH와 유사한 결과를 보였다. 기계적인 텍스쳐 분석결과, 전반적인 항목평가결과 자색양파를 첨가한 설기가 대조군에 비해 낮은 값을 나타내었으나 부착성은 냉동자색양파첨가 설기군이 대조군보다 높은 값을 나타내었고 저장기간이 늘어남에 따라 전반적인 항목평가에서 유의적인 증가세를 나타내었다. 관능적 품질 특성은 실온보관 자색양파 첨가량이 많을수록 색, 향, 질감, 맛 및 전반적인 기호도에서 유의적인 증가세를 나타내었고, 냉동보관 자색양파 첨가량이 늘수록 모든 항목에서 유의적으로 감소하였다. 이상의 연구를 통해 실온보관 자색양파 30% 첨가한 설기를 가장 선호하는 것으로 나타났다. 이상의 결과로부터 색감, 향미, 질감 및 전반적인 만족도를 고려한 자색양파설기 제조 시 실온에서 보관한 자색양파를 30% 수준으로 첨가하는 것이 현대인의 기호에 맞는 건강떡으로서의 이용 가능성을 확인하였으며 약리활성이 우수한 건강 기능성 음식으로 대중화될 수 있으리라 판단되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제17권5호
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• pp.612-616
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• 2004

This study was carried out to compare the semen characteristics, frozen-thawed sperm viability and testosterone concentration and in vitro fertilization (IVF) and development of in vitro matured pig oocytes between two Yorkshire boars. Semen and blood samples were collected once per week from October to November 2002 from two adult Yorkshire boars at 18 months of age with 170 kg body weight. Sperm were deep frozen in 5 ml maxi-straws with lactose-egg yolk and N-acetyl-D-glucosamine (LEN) diluent and stored in liquid nitrogen. Blood samples were obtained at 10 a.m. by inserting a 21 gauge, hypodermic needle attached to 10 ml syringe into surface veins in the ear. The concentration of testosterone was determined by Competitive Enzyme Immunoassay. Ovaries were collected from prepubertal gilts at a local slaughter house. Cumulus oocyte complexes were aspirated from antral follicles (3 to 6 mm in diameter). The medium used for oocyte maturation was modified TCM 199. After about 22 h of culture, oocytes were cultured without cysteamine and hormones for 22 h at $38.5^{\circ}C$, 5% $CO_2$ in air. For IVF, one frozen 5 ml straw was thawed at $52^{\circ}C$in 40 sec and was diluted with 20 ml Beltsville thawing solution at room temperature. Sperm were washed 2 times in mTLP-PVA and inseminated without preincubation after thawing. Oocytes were inseminated with $2{\times}10^7$/ml sperm concentration. Oocytes were coincubated for 6 h in 500 ${\mu}$l mTBM fertilization medium. At 6 h after IVF, oocytes were transferred into 500 ${\mu}$l NCSU-23 culture medium for further culture of 48 and 144 h. There were no significant differences in the semen volume, motility, normal acrosome morphology and sperm concentration of raw semen between A and B of Yorkshire boar. However, motility and normal acrosome of boar A were higher than those of boar B at 0.5, 2, 3, 4, 5 and 6 h incubations of frozen-thawed sperm. Testosterone concentration (3.75 ng/ml) of boar A was higher than that (2.34 ng/ml) of boar B. The rate of blastocyst formation (15.1%) of boar A was higher than that (10.4%) of boar B. In conclusion, serum testosterone concentration of boar showed very important role for the frozen-thawed sperm viability and the blastocyst formation of pig oocytes matured in vitro.

• 한국식품과학회지
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• 제14권4호
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• pp.324-329
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• 1982

고등어를 이용하여 어육냉동 steak을 만들어 이것의 가공조건을 구명하고 또한 대두단백질의 첨가가 제품품질에 미치는 효과에 대하여 검토하였다. 고등어 냉동 steak를 가공하기 위해서는 어육에 탄산수소나트륨 0.5%, 식염 1.0%, 중합인산염 0.2%, 글루탐산나트륨 0.2%, 설탕 2.0% 및 향신료로서 고춧가루 0.2%, 후추가루 0.4%, 마늘가루 0.2%와 nutmeg 0.2%를 첨가하여 고기갈이한 다음 $-3^{\circ}C{\sim}-5^{\circ}C$에서 2일간 조온저장 후 동결저장하는 것이 가장 좋았다. 또한 대두단백질을 5% 첨가하므로서 제품의 동결저장중 색조변화, 유리드립생성, 지방산패억제 및 텍스튜어의 개선효과가 있었으며 관능검사 결과로서도 고등어냉동 steak는 동결저장 90일동안 품질이 안정되게 유지되었다.

• 한국응용과학기술학회지
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• 제8권1호
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• pp.69-78
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• 1991

Frog legs were stored before and after 30, 60, 90, 120 days at $-30^{\circ}C$ and $-70^{\circ}C$. The amounts of triglycerides, glycolipide and phospholipids by TLC and composition of fatty acids by GC were shown in this experiment, there fore, I have got these following results. 1. The amount of PL in frog legs consisted of 80% of the total lipids before storage. As the days of storage was longer, the amount of TG was increased, while PL and GL were decreased. 2. TG and ES & HC were composed of 66% to 77% of the neutral lipids composition before storage. As the days of storage was longer, the amount of MG, DG. FFA and ES & HC were increased, while FS and TG were decreased. 3. PE and PC were composed of 78% to 81% of the phospholipids composition before storage. As the days of storage was longer, the amount of FA and PS were increased, while PC and PE were decreased. 4. The composition of fatty acids in neutral lipids of frozen frog legs at $-30^{\circ}C$, 120 days was studied, Capric acid. Stearic acid, Behnic acid. Oleic acid and Linoleic acid were increased as compared with initial neutral lipids.

• 한국축산식품학회지
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• 제18권1호
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• pp.1-8
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• 1998

This study was conducted to investigate the effects of $\alpha$-, ${\gamma}$-, $\delta$-Tocopherols on oxidative stability of lipid in hamburger patties during frozen storage. Hamburger patty samples were prepared with the addition of tocopherol by fat basis: control, treat 1 (control+0.02% $\alpha$-tocopherol), treat 2 (control+ 0.02% ${\gamma}$-tocopherol were cooked at 85$^{\circ}C$ for 3 minutes and the stored at -1$0^{\circ}C$ or 3$0^{\circ}C$). The fatty acids composition of the hamburger samples were analyzed during the storage periods (1, 20, 40, 60 and 80 days). there was no difference in the fatty acids composition among control and treatment groups in early storage time; however, content of unsaturated fatty acid was decreased in control group and slightly decreased or almost not changed in treatment groups as the storage period passed. No difference in the ratio of saturated fatty acid : unsaturated fatty acid (SFA : USFA) and saturated fatty acid : monounsaturated fatty acid : unsaturated fatty acid (SFA : MUFA : PUFA) between control and treatment groups at early storage time. However, SFA : USFA and SFA : MUFA : PUFA was decreased in control and slightly decreased or not changed in all treatment groups as the storage period passed.

• 한국수정란이식학회지
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• 제9권3호
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• pp.269-277
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• 1994

The objective of this study was to develop an effective in vitro production system capable of obtaining more porcine embryos from immature oocytes These experiments were conducted to examine the effect of sperm factor on the IVF and IVD, and the effect of coculture with somatic cells on the IVD of embryos. Although the concentration of epididymal sperm for IVF did not affect on cleavage rate, but 5 x 105 sperm/mi showed the highest cleavage rate(48.7%) and the developmental potential of IVF oocytes from this concentration was also greatly higher (P$^{\circ}C$-stored sperm for l2hrs and the cleavage rate from fresh sperm was significantly higher (P<0.05) than that from frozen sperm, but the developmental potential after IVF was slightly high from the frozen sperm. The cleavage rate of IVF oocytes cocultured with oviductal epithelial cells and cumulus cells was 76.3% and 72.9%, respectively. There was no difference between two coculture systems but this rate was significantly higher(P<0.05) than that of medium alone(42.0%).

• 한국식생활문화학회지
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• 제17권1호
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• pp.90-95
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• 2002

This study was conducted to determine the desirable thawing method and storage time of Nokchaborijook(gruel made of barley, rice powder and Green tea) which made of optimum conditions as the 5.8% Green tea and 15 seconds grinding time. Decision on desirable storage time(10, 20, 30, 40, 50days), thawing method(water bath, microwave oven) of frozen Nokchaborijook were determined by sensory evaluation and physicochemical attributes. As the storage time became longer, moisture and flavonoid contents were decreased. Viscosity, the ratio of water seperation, spreadability of Nokchaborijook which stored at 30 days showed great differences between the two thawing method. Among the sensory characteristics, desirability for overall, flavor, and texture were decreased as the storage time became longer. As a result of consumer test, Nokchaborijook thawed at water bath was more desirable than Nokchaborijook thawed at microwave oven. According to the results, frozen Nokchaborijook can be using as a supplement of dietary fiber and flavonoid for the health and be using convenience food.

• 한국가축번식학회지
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• 제8권2호
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• pp.79-86
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• 1984

This cepseiment was carried out to cerify the effect of thawing methods and preservative temperature on the sperm motility and fertility after thawing semen with plastic straws in fresh and warm water. Sperm motility in vitro stored at room temperature after thawing were conducted by the various storage hours. A field trial after thawing semen with warmed water in straws from Cheju native cows involving 4 technicians and 800 cows first (or second) services gave the following results. The thawing methods of warmed water for one minute in semen motility were considerably higher than that in iced water during 12 hours after thawing semen, however, the sperm survival index of ice-water shwed a better results according as the time passed away, but not significant differences. Preservative temperaure at 5$^{\circ}C$ (iced water) after thawing gave significantly better results than that of thawed at 3$0^{\circ}C$ (warmed water). The N R rate to 175 inseminations with semen thawed at 15-2$0^{\circ}C$ (fresh water) was 82.8%, 80.9% for 610 inseminations thawed in warm water. Conception rate ofthe semen thawed in warm water for 10-60 secs gave no significant difference among storage hours, because the semen used to be inseminated within one hour almost, but in decreased when semen thawed at the period of one minute over.

• 한국식품위생안전성학회지
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• 제14권1호
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• pp.27-33
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• 1999

Phytic acid, making up 1~5% of the composition of many plant seeds and cereals, is known to form iron-chelates and inhibit lipid peroxidation. Thiobarbituric acid reactive substances (TBARS), as an indication of lipid peroxidation, were measured in beef round, chicken breast, pork loin, and halibut muscle after the meats were stored for 0, 1, 3, 5, and 7 days at various temperatures [frozen (~2$0^{\circ}C$), refrigerator (4$^{\circ}C$), and room temperature ($25^{\circ}C$)]. Phytic acid effectively inhibited lipid peroxidation in beef round, chicken breast, halibut, and pork loin muscle (p<0.05). The inhibitory effect of phytic acid was dependent on concentration, storage time, and temperature. At frozen temperature, the inhibitory effect of phytic acid was minimal, whereas at room temperature, the inhibitory effect of phytic acid was maximal, probably due to the variation of the control TBARS values. At the concentration of 10 mM, phytic acid completely inhibited lipid peroxidation in all the muscle foods by maintaining TBARS values close to the level of the controls, regardless of storage time or temperature (p<0.05). The rate of lipid peroxidation was the highest in beef round muscle, although they had a close TBARS value at 0 day. Addition of phytic acid to lipid-containing foods such as meats, fish meal pastes, and canned seafoods may prevent lipid peroxidation, resulting in improvement of the sensory quality of many foods and prolonged shelf-life.