• Earthquakes and Structures
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• v.13 no.5
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• pp.429-442
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• 2017

Dry bridges have been widely applied in the Qinghai-Tibet Railway (QTR) to minimize the thermal disturbance of engineering to the permafrost. However, because the Qinghai-Tibet Plateau is an area with a high potential occurrence of earthquakes, seismic action can easily destroy the dry bridges. Therefore, a three-dimensional numerical model, with consideration of the soil-pile interactions, is established to investigate the thermal characteristics and their impact on the seismic response of the dry bridge in permafrost region along the QTR. The numerical results indicate that there exist significant differences in the lateral displacement, shear force, and bending moment of the piles in different thermal conditions under seismic action. When the active layer become from unfrozen to frozen state, the maximum displacement of the bridge pile reduces, and the locations of the zero and peak values of the shear force and bending moment also change. It is found that although the higher stiffness of frozen soil confines the lateral displacement of the pile, compared with unfrozen soil, it has an adverse effect on the earthquake energy dissipation capacity.

• Journal of the Korean Geosynthetics Society
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• v.13 no.4
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• pp.45-56
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• 2014

Frozen ground in cold region consists of an upper active layer and lower permafrost which is permanently frozen land. During the summer season, the air temperature is high enough to make the frozen ground melt, which causes the reduction of soil strength and thaw settlement. These phenomena result in structural instability, so it is necessary to apply frozen ground stability techniques. Thermosyphon is a closed natural two-phase convection device to maintain the ground temperature below $0^{\circ}C$ by extracting heat from the ground and discharges it into the atmosphere. Experimental and numerical investigation has been performed to estimate the effect of the refrigerant filling ratio in thermosyphon using R-134a refrigerant and the thermal conductance of the thermosyphon.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.24 no.7 s.178
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• pp.1787-1794
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• 2000

• Geophysics and Geophysical Exploration
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• v.16 no.1
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• pp.63-66
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• 2013

• Transactions of Materials Processing
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• v.15 no.5 s.86
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• pp.347-353
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• 2006

The delivery system such as sprue, runner and gate is a waste of resin in injection molding operation. In this study the reduction of runner size has been investigated using injection molding CAE softwares, Moldflow and Moldex3D, and commercial CFD Softwares, Fluent and Polyflow. To verify the computational results experiment was performed. There were three considerations in deciding optimal runner size in this study: minimum pressure at the gate that makes resin fully filled in the cavity, minimum runner size that compensates shrinkage of resin in the cavity, and frozen layer thickness formed in the runner during injection. Through the computer simulations the optimal runner size that satisfies those three considerations has been decided. Although the computational results among the softwares were slightly different, it was enough to predict the optimal runner size. The previous runner diameter was 8 mm and predicted optimal size was 5 mm. This was verified by injection molding experiment. Thus, the way of CAE application in deciding optimal runner size adapted in this study would be appropriated.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2006.05a
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• pp.363-366
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• 2006

The delivery system such as sprue, runner and gate is a waste of resin in injection molding operation. In this study the reduction of runner size has been investigated using injection molding CAE softwares, Moldflow and Moldex, and commercial CFD Softwares, Fluent and Polyflow. To verify the computational results experiment was performed. There were three considerations in deciding optimal runner size in this study: minimum pressure at the gate that makes resin fully filled in the cavity, minimum runner size that compensates shrinkage of resin in the cavity, and frozen layer thickness formed in the runner during injection. Through the computer simulations the optimal runner size that satisfies those three considerations has been decided. Although the computational results among the softwares were slightly different, it was enough to predict, the optimal runner size. The previous runner diameter was 8 mm and predicted optimal size was 5 mm. This was verified by injection molding experiment. Thus, the way of CAE application in deciding optimal runner size adapted in this study would be appropriated.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.9 no.4
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• pp.435-445
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• 1997

It was studied the phenomena of transient freezing of an inclined water-saturated enclosure. One side of the test section was cooled and the other sides were insulated. The effects of the initial temperature, the inclination angle on the temperature field and the shape of the ice-water interface were observed. In the beginning of freezing, with increasing the inclination angle the freezing rate was increased and in the stable density layer centered $4^{\circ}C$, the freezing was fast as the convective fluid flow became small. When the initial temperature was above the $4^{\circ}C$, the frozen thickness in the upper part of inclined surface was thinner than that in the lower part, but with time the frozen thickness of upper part was thicker than that of lower part, below the $4^{\circ}C$, the frozen thickness in the upper part was thicker than that of lower part from the begining, and above the $8^{\circ}C$ in the beginning upper part was thinner with concave, but with time thicker the upper part, vanishing concave.

• Clinical and Experimental Reproductive Medicine
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• v.28 no.1
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• pp.33-40
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• 2001

Objective: This study was to establish the human embryonic stem (ES) cells derived from frozen-thawed blastocyst stage embryo that were destined to be discarded after five years in routine human IVF-ET program. Methods: Frozen-thawed and survived human blastocysts were treated by immunosurgery, and recovered ICM cells were cultured onto STO feeder cell layer and ICM colony was subcultured by mechanical dissociation into clumps. To identify ES cell, alkaline phosphatase staining and expression of Oct4 in replated ICM colonies were examined. Also, to examine the possibility of ES cell differentiation, retinoic acid (RA), basic fibroblast growth factor (b-FGF), nerve growth factor (NGF) were added in culture medium. In addition, to classify the specific cell type, differentiated cells were stained by indirect immunocytochemistry. Results: One ICM colony recovered from frozen-thawed six blastocysts was subcultured, continuously replated during 40 passage culture duration without differentiation. Subcultured colonies were strong positively stained by alkaline phophatase. When the expression of Oct4 in cultured ES colony was examined, Oct4b type is more clearly indicated than Oct4a one although there was not detected in embryoid body or differentiated cells. In differentiated cardiomyocytes from ES colony, cells were beaten regularly (60 times/min). In differentiated neural cells from ES colony, neurofilament (NF) 200 kDa protein, microtubule associated protein (MAP) 2 and ${\beta}$-tubulin of specific marker in neurons, glial fibrillary acidic protein (GFAP) of specific marker in astrocytes and galactocelebrocide (GalC) of specific marker in oligodendrocytes were confirmed by indirect immunocytochemistry. Also, muscle cells were detected by indirect immunocytochemistry. In addition, ES colonies can be successfully cryopreserved. Conclusion: This study suggested that establishment of human ES cells can be successfully derived from frozen-thawed blastocysts that were destined to be discarded, and obtained specific cell types (cardiomyocytes, neurons and muscle cells) through the in vitro differentiation procedures of ES cells.

• Journal of Climate Change Research
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• v.5 no.3
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• pp.249-256
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• 2014

Permafrost is frozen soil below $0^{\circ}C$ for two or more years. Surface of permafrost is called as active layer that seasonally thaws during the summer. Although the thawing of permafrost may deepen the active layer and consequently increase the microbial activity, the microbial community structure in this habitat has not yet been well described. In this study, we presented bacterial and archaeal diversity in the active layer soil from Resolute, Canada using pyrosequencing analysis. The soil sample was collected from the surface of the marsh covered with moss and Carex. A total of 7,796 bacterial reads for 40 phyla and 245 archaeal reads for 4 phyla were collected, reflecting the high diversity of bacteria. Predominant bacterial groups were Proteobacteria (37.7%) and Bacteroidetes (30.0%) in this study. Major groups in Archaea were Euryarchaeota (51.4%) and Thaumarchaeota (46.1%). Both methane producing archaea and consuming bacteria were detected in this study. Although it might be difficult to characterize microbial community with only one sample, it could be used for the basis of assessing the relative importance of the specific groups with a high resolution on the bacterial and archaeal community in this habitat.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.2 no.2
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• pp.105-111
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• 1990

The drying rate in freeze drying was obtained by experiment of garlic moisture contents depending on the drying time. Freeze drying experiment of garlic juice was carried out in vaccum freeze drier of laboratory scale by backface heating, and a mathematical model is also used to simulate the process of simultaneous heat and mass transfer in freeze drying to compare with experimental data.