• Title/Summary/Keyword: Frog

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First Record of a Brown Frog Rana huanrenensis (Family Ranidae) from Korea

  • Yang, Suh-Yung;Kim, Jong-Bum;Min, Mi-Sook;Suh, Jae-Hwa;Kang, Young-Jin;Matsui, Masafumi;Fei, Liang
    • Animal cells and systems
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    • v.4 no.1
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    • pp.45-50
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    • 2000
  • We found a brown frog species, which is unrecorded from South Korea. Rana huanrenensis Fei, Ye, and Huang (1990), This species was originally described from northeastern China. In having 2n=24 chromosomes, this species is closely related to Rana dybowskii, R. chensinensis, R. ornativentris, R. pirica, and Chinese R. huanrenensis, but it is different from the first four species in the ecological, morphological, and genetic characteristics. By contrast, this species Is identical to Chinese R. huanrenensis In the habitat of montane stream-breeding, absence of the vocal sac, and genetic properties. This record is a significant range extention of R. huanrenensis.

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PCR Detection of Ranavirus in Gold-spotted Pond Frogs (Rana plancyi chosenica) from Korea

  • Kim, Suk;Sim, Mi-Yeong;Eom, Ahn-Heum;Park, Dae-Sik;Ra, Nam-Yong
    • Korean Journal of Environmental Biology
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    • v.27 no.1
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    • pp.110-113
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    • 2009
  • In recent years, there has been a rapid global decline in amphibian populations, and infectious diseases have been associated with this decline. Diseased Gold-spotted pond frogs (Rana plancyi chosenica) were collected from a frog farm in Korea and identified using morphological and molecular analysis to identify the disease. The typical symptoms of ranaviral infection were observed in the tadpoles and adults frogs. The nucleotide sequence analysis revealed that the sequences showed the closest similarity with sequences to Frog virus 3, which belongs to the genus Ranavirus.

The Interpretaion of the Fairy Tale <The Frog King or Iron Heinrich> in Light of Jungian Psychology : The Unification of Opposites in the Fairytale (민담 <개구리 왕 혹은 충직한 하인리히>의 융심리학적 해석 : 민담에 나타난 대극의 합일)

  • Boseop Lee
    • Sim-seong Yeon-gu
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    • v.36 no.1
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    • pp.55-86
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    • 2021
  • The initial situation in our tale shows that the earth-mother-feminine principle disappeared from the center of the collective consciousness into the collective unconscious. Therefore the heaven-father-masculine principle is dominant, which is represented by the king. And in the king's daughter, who is living without mother, the positive father complex is working. She stays in the heaven-spirit world playing with the golden ball, which can be seen as the state of inflation. She is disconnected from the earth-mother-feminine principle, which is important for a woman to find her genuine feminine identity. This demanded principle approaches her through the frog, a bewitched prince. Psychologically it means that a man is under the power of the negative mother complex. The disgusting, ugly frog is a symbol for the shadow, the earthly animal instincts of the princess. Only with his help she can find her golden ball again, which has fallen into the deep well. Their talk about the rewards to him for his help shows us very well the opposites. The frog wants the feminine value such as relation, earthly eros, but the princess offers the masculine value such as heavenly logos. After the frog brought her the lost ball, i.e. she regained her libido, she completely forgot her promise. Like this the content, which is becoming conscious, here the shadow, is easy to fall back into the unconscious and to be repressed. The frog cannot be with the princess without the help of the king, a father figure, a firm protector of the collective oder. At first unwillingly the princess obeys Logos of her father. But her authentic instinctual urge grows stronger and it causes that her ego is released from the power of her father complex. At just this moment the frog turns into a prince, i.e. he is liberated from the mother complex. The marriage of princess and frog-prince symbolize the unification of the opposites: heaven becomes earthly and earth becomes heavenly. Three iron bands, wrapped around the heart of Heinrich, a young king's servant, are snapped, while he brings the prince and princess back to his kingdom. The heart, the place of earth-mother-feminine consciousness, is now liberated. This principle, which disappeared into the unconscious, emerged into the collective consciousness and the wholeness is recovered. The Self is now leading the collective consciousness, which includes not only the principle of Logos but also Eros.

Bioacoustic Change of Dybowski's Brown Frog by Highway Noise (고속도로 소음에 의한 북방산개구리의 생물음향학적 특성 변화 연구)

  • Ki, Kyong-Seok;Sung, Chan-Yong
    • Korean Journal of Environment and Ecology
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    • v.28 no.3
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    • pp.273-280
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    • 2014
  • This study examined whether Dybowski's brown frogs(Rana dybowskii) in noisy highway roadsides had different mating calls from those in natural sites. We selected four study sites: two rice paddy sites in Youngdong Highway roadside and two nearby natural counterparts. Frog calls were recorded between 18:30 and 20:00 on February 24, February 27, and March 14, 2014. Frog calls in the natural sites had fundamental frequency approximately at 700 Hz with two to four apparent harmonics, while frog calls in the highway sites had higher fundamental frequency with up to seven apparent harmonics. Analysis of variance (ANOVA) shows that a roadside site that are directly exposed to highway noise had statistically higher frog calling frequency than other study sites. However, the higher calling frequency was not found in another roadside site that differed in elevation from the highway and was buffered by forests. These results indicate that male frogs in a noisy highway roadside called females with a higher pitch and more apparent harmonics to avoid being masked by highway noise. These results also suggest that there is a threshold noise level that interrupts frog's mating behavior and it is needed to maintain highway roadside noise to this threshold level.

Toxicity Evaluation of Chemicals using Black-spotted Pond Frog Embryos, Rana nigromaculata (참개구리 배아를 이용한 화학물질의 독성평가 연구)

  • Ko, Sun-Kun
    • Korean Journal of Environmental Biology
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    • v.30 no.3
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    • pp.231-237
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    • 2012
  • Toxicity of $Ni^{2+}$ and Tebuconazole were investigated via FETAX (Frog Embryo Teratogenesis Assay-Xenopus) protocol using domestic frog embryos. Embryos of Black-spotted pond frog, Rana nigromaculata, were incubated and toxic effects of $Ni^{2+}$ and Tebuconazole were investigated by probit analysis. As a result, mortality and malformation rates were increased and larval body length was decreased in a dose dependant manner of $Ni^{2+}$ and Tebuconazole. The half maximal effective concentration ($EC_{50}$) of $Ni^{2+}$ and Tebuconazole were 0.07, $12.7mg\;L^{-1}$, respectively and the half maximal lethal concentration ($LC_{50}$) of $Ni^{2+}$ and Tebuconazole were 4.2, 39.1, respectively. The teratogenic index (TI) were 61.4 in $Ni^{2+}$ and 3.1 in Tebuconazole, respectively. These results reveals that $Ni^{2+}$ and Tebuconazole suppress the development of Black-spotted pond frog embryos at the low concentration as showing teratogenic effects in other assay system. Therefore, teratogen assay system using the Rana nigromaculata embryos could be useful as a tool to evaluate the toxicity of the pollutants in environment.

Application of Practical Scheme for Analysis of Tsunamis - Busan New Port Area (지진해일 해석을 위한 실용적인 기법의 적용 - 부산 신항만 지역)

  • Choi, Moon-Kyu;Cho, Yong-Sik
    • 한국방재학회:학술대회논문집
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    • 2007.02a
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    • pp.395-398
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    • 2007
  • In this study, new dispersion-correction terms are added to leap-frog finite difference scheme for the linear shallow-water equations with the purpose of considering the dispersion effects of the linear Boussinesq equations for the propagation of tsunamis. The new model is applied to near Gadeok island in Pusan about The Central East Sea Tsunami in 1983 and The Hokkaldo Nansei Oki Earthquake Tsunami in 1993 one simulated in the study.

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Seasonal Variation in the $Na^+$,$K^+$-ATPase Activity in Frog (Rana dybowskii) Brain (개구리 뇌에서 $Na^+$,$K^+$- ATPase 특성의 계절적 변화)

  • 김미승;임욱빈
    • The Korean Journal of Zoology
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    • v.38 no.4
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    • pp.449-456
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    • 1995
  • Seasonal changes in the activity and charaderistics of brain Na+, K+-ATPase and Mg2+-AWase were investigated in frog (Rana dybowskii) The brain Na+, K+-ATPase adivity during hibernation was similar to that in active period in frogs. The Na+, K+-AWase activity increased in December and March, when the frogs enter into and awake from the hibernation. Over 5-35$^{\circ}C$ temperature range, Na+, K+-ATPase showed non4inear Arrhenius kinetics throughout the year. The brain Mg2+-ATPase activity decreased during hibernation, but markedly increased in March. The Arrhenius plots for Mg2+-AWase activity were linear in frogs both in torpid and active state. The ratio of Na+, K+-AWase activity at 15~C to at 35~C did not change during hibernation. The sensitivity of Na+, K+-AWase to ouabain was also unchanged throughout the year. These results indicate that the activity and charaderistics of brain Na+, K+-AWase remain unchanged during hobernadon in frog.

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Genetic Diversity among Local Populations of the Gold-spotted Pond Frog, Rana plancyi chosenica (Amphibia: Ranidae), Assessed by Mitochondrial Cytochrome b Gene and Control Region Sequences

  • Min, Mi-Sook;Park, Sun-Kyung;Che, Jing;Park, Dae-Sik;Lee, Hang
    • Animal Systematics, Evolution and Diversity
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    • v.24 no.1
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    • pp.25-32
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    • 2008
  • The Gold-spotted pond frog, Rana plancyi chosenica, designated as a vulnerable species by IUCN Red list. This species is a typical example facing local population threats and extinction due to human activities in South Korea. A strategic conservation plan for this endangered species is urgently needed. In order to provide information for future conservation planning, accurate information on the genetic diversity and taxonomic status is needed for the establishment of conservation units for this species. In this study, we used a molecular genetic approach using the mitochondrial cytochrome b gene and control region sequences to find the genetic diversity of gold-spotted pond frogs within South Korea. We sequenced the mitochondrial DNA cytochrome b gene and control region of 77 individuals from 11 populations in South Korea, and one from Chongqing, China. A total of 15 cytochrome b gene haplotypes and 34 control region haplotypes were identified from Korean gold-spotted pond frogs. Mean sequence diversity among Korean gold-spotted pond frogs was 0.31% (0.0-0.8%) and 0.51% (0.0-1.0%), respectively. Most Korean populations had at least one unique haplotype for each locus. The Taean, Ansan and Cheongwon populations had no haplotypes shared with other populations. There was a sequence divergence between Korean and Chinese gold-spotted pond frogs (1.3% for cyt b; 2.9% for control region). Analysis of genetic distances and phylogenetic trees based on both cytochrome b and control region sequences indicate that the Korean gold-spotted pond frog are genetically differentiated from those in China.

Detection of Gnathostoma spinigerum Advanced 3rd-Stage Larvae in the Chinese Edible Frog, Hoplobatrachus rugulosus, from Local Markets in Phnom Penh, Cambodia

  • Sohn, Woon-Mok;Jung, Bong-Kwang;Hong, Sooji;Ryoo, Seungwan;Lee, Keon Hoon;Khieu, Virak;Chai, Jong-Yil
    • Parasites, Hosts and Diseases
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    • v.59 no.5
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    • pp.519-522
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    • 2021
  • The Chinese edible frogs, Hoplobatrachus rugulosus (n=20), and the striped snakehead fish, Channa striata (n=34), were purchased from local markets in 3 administrative regions of Cambodia (Phnom Penh, Pursat, and Takeo Provinces) from May 2017 to April 2019, and their infection status with Gnathostoma sp. larvae was investigated. The frogs and fish were transported to the laboratory with ice and examined using the artificial digestion method. Advanced 3rd-stage larvae (AdL3) of Gnathostoma spinigerum, 24 in total number (1-6 larvae/frog), were detected from 6 (60.0%) out of 10 frogs purchased from Phnom Penh. No gnathostome larvae were detected in 10 frogs purchased from Takeo Province and 34 snakeheads from Phnom Penh, Pursat, and Takeo Provinces. AdL3 isolated from the frogs were 2.55-3.90 mm long and 0.31-0.36 mm wide. They had a characteristic head bulb (0.081×0.191 mm in average size) with 4 rows of hooklets, a muscular long esophagus (0.950-1.230 mm long), and 2 pairs of cervical sacs (0.530-0.890 mm long). The average number of hooklets in the 1st, 2nd, 3rd, and 4th rows was 41, 45, 48, and 51, respectively. These features were consistent with G. spinigerum AdL3. By the present study, it has been first confirmed that the Chinese edible frog, H. rugulosus, from Phnom Penh serves as a second intermediate host for G. spinigerum, although their intensity of infection was not so high compared to other previously reported localities.