• 지역사회간호학회지
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• 제27권4호
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• pp.346-357
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• 2016

Purpose: This study attempted to investigate the prevalence and related factors of Clonorchiasis among five major riverside residents in South Korea. Methods: This study is descriptive research, nationwide survey, and the subjects are 23,492 residents selected by convenience sampling. Data collection was conducted between March 1 and June 30, 2011, and stool collection and questionnaire survey were conducted by affiliated public health centers in 38 cities and Gun's. Results: The prevalence rates of Clonorchiasis in the five major riversides were as follows: the Guem River 15.2%; the Nakdong River 11.9%; the Seomjin River 10.9%; the Han River 5.7%; and the Yeongsan River 3.9%. The prevalence rates were shown to be significantly high among people who had highly frequent experiences of eating and cooking freshwater raw fish, were diagnosed with liver and/or biliary tract diseases, and drank less than once a month. Conclusion: These results suggest that it is necessary to improve awareness of Clonorchiasis and provide intensive public health education for the riverside residents. And the target groups should be set up by reflecting the characteristics of at-risk groups, and it is necessary to prepare customized strategies for prevention and management of Clonorchiasis.

• 한국수산과학회지
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• 제49권2호
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• pp.154-160
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• 2016

Several our studies have focused recently on the mass production of the freshwater Cladoceran Moina macrocopa which can substitute Artemia nauplii for the culture of larval marin fish. A 6 weeks experiment was conducted to investigate the effects of enrichment on the fatty acid composition of Moina macrocopa through feeding Schizochytrium sp. containing highly unsaturated fatty acids and to study the impacts of n-3-HUFA enriched Moina on improving survival rate and fatty acid compostion of larval rockfish Sebastes schlegeli. After feeding for 6 weeks, the Moina-fed fry resulted in a higher survival rate of 99.2% compared to the Artemia-fed fry 12.8%. In addition, the Moina-fed fry had the fast growth rate 45.6mm compare to the Artemia-fed fry 25.7 mm at the end of the experiment. The Moina-fed fry showed significantly higher level of 16.47% DHA than their Artemia-fed fry counterparts of the level of 3.97% with respect to DHA. PL, the cell membrane components in living food organisms, constituted 63.8% of the Moina, which was significantly higher than in the 40.1% of the Artemia. The present study indicate that Moina macrocopa can be used as Artemia substitute and improving the survival rate rockfish larvae through enchriment Schizochytrium sp.

• Parasites, Hosts and Diseases
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• 제32권4호
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• pp.267-270
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• 1994

충청북도 중원군 달천강 유역 주민의 기생충 감염상을 알아보기 위하여 1993년 8월부터 9월까지 이류면 수주리 마을주민 67명을 대상으로 대변검사를 실시하고. 감염원 조사를 위하여 마을앞 달천강 상류지역의 담수어를 투망으로 채질하여 조사하였다. 조사결과 간흡충 양성자가 67명중 22명 (32.8%)이었고 메타고니무스 양성자가 14명(20.9%)이었다 조사한 담수어 17종중 간흡충은 14종이, 메타고니무스는 13종이 감염되어 있었다. 간흡충의 경우 큰납지리, 모래무지. 꼬치동자개. 눈동자개 등이 높은 감염률을 보였고, 메타고니무스의 경우는 참마자, 큰납지리. 모래무지 피라미 등이 높은 감염률을 보였다. 메타고니무스 양성자로부터 회수한 성충은 Miyata형이었고 피라미에서 얻은 피낭유충을 마우스에 실험감염시켜 회수한 성충도 역시 Miyata형이었다. 위의 결과로 달천강 유역에 담수어를 매개로 한 간흡충과 메타고니무스(Miyata형) 감염이 고율로 유행함을 확인하였다.

• Parasites, Hosts and Diseases
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• 제55권3호
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• pp.287-294
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• 2017

The present study was performed to record new definitive hosts of Isthmiophora hortensis, and to describe morphological characteristics derived from a variety of worm samples for clarification of its taxonomic validity. Morphological characteristics with dimensions were observed in worm samples (n=21) from naturally infected wild animals, including a raccoon dog Nyctereutes procyonoides from Gimhae-si (City), Gyeongsangnam-do, stray cats and a striped field mouse from several localities, and a wild boar Sus scrofa, from Gurye-gun (County), Jeollanam-do. In addition, adult flukes (n=45) recovered in albino rats experimentally infected with the metacercariae from a freshwater fish species were also subjected to morphological studies. The mean ratios of the body length (BL) to body width (BW) were 5.86 and 5.76 in worms from wild animals and experimental rats, respectively. Those of the ventral sucker to oral sucker were 2.92 and 3.01 in worms from 2 groups. The mean percentages of the hindbody length (HBL) to BL were 42.1 and 41.2 in 2 groups. Those of uterine fields to BL were 9.8 and 12.2 in the 2 worm groups. By the present study, the 2 species of wild animals, the raccoon dog and wild boar, have been added as new definitive hosts for I. hortensis. The morphological characteristics of adult flukes derived from a variety of host source were redescribed to support the taxonomic validity of this echinostome species.

• Animal cells and systems
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• 제6권1호
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• pp.45-51
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• 2002

We compared the complete mitochondrial cytochrome b gene sequences of Korean and European cobitids to provide independent evidence for assessment of systematic and biogeographic relationships of species in the genus Cobitis. The data suggested monophyly of the genus Cobitis and the inclusion of Korean Cobitis species within the group having one lamina circularis, a primitive condition. Also, all the phylogenetic analyses using maximum parsimony, maximum likelihood, and neighbor joining methods showed a monophyletic relationship among Cobitis. The basal position of the Caspian C. cf. sibirica reported here reflects the eastern Asiatic origin cf. the European Cobitis and establishes C. cf. sibirica as an independent lineage. The Korean C. pacifica diverged next to C. cf. sibirica in basal group from the genus Cobitis. This result is in agreement with the hypothesized Asiatic origin of some European freshwater fish lineages. The phylogenetic relationships in this study showed a close affinity between C. zanadreai and C. sinensis. Two new species, C. tetralineata and C. pacifica in Korea also are closely related to monophyletic group clustering the type species of the Acanestrinia subgenus (C. elongata) with all the endemic Italian species (C. bilineata and C. zanandreai). This may suggest that the affinity between the Korean and Danubian-Italian imply genetic convergence or genetic plesiomorphic state between allopatric species that are separated for the Miocene. The mtDNA-based phylogeny for the species of the genus Cobitis from Kores and Europe permits phylogenetic assessment of the morphological transitions of Iamina circularis.

• Parasites, Hosts and Diseases
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• 제48권4호
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• pp.339-342
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• 2010

Phongsaly Province, located in the northernmost area of Lao PDR, was previously suggested to be endemic for the liver fluke Opisthorchis viverrini infection. To confirm, or rule out, this suggestion, the Phonxay village in the Khoua District, Phongsaly Province, was selected for a survey. Ten volunteers (8 men and 2 women aged 31-57 years) who consumed raw freshwater fish and had gastrointestinal troubles were treated with a single dose of praziquantel (40 mg/kg) and pyrantel pamoate (10 mglkg) and purged with magnesium sulfate to recover any worm parasites. Eight of the 10 volunteers expelled 1 or more species of trematodes, nematodes, or cestodes (worm positive rate; 80%). The worms were morphologically identified as H. taichui (861 worms from 8 people), H. yokogawai (59 from 6 people), Phaneropsolus bonnei (1 from 1 person), Trichostrongylus sp. (2 from 2 people), Ascaris lumbricoides (2 from 1 person), Enterobius vermicularis (11 from 3 people), and Taenia saginata (1 strobila with scolex from 1 person). The results indicate that the mountainous area of Phongsaly Province, Lao PDR, is not endemic for the liver fluke but endemic for intestinal flukes, in particular, Haplorchis taichuiand H. yokogawai.

• Asian Pacific Journal of Cancer Prevention
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• 제17권4호
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• pp.1811-1815
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• 2016

Wetland geographical areas have a higher incidence of Opisthorchis viverrini-associated cholangiocarcinoma (CCA), confirmed by data from geographic information systems, than other areas. Behavioral data also indicate that people in these areas traditionally eat uncooked freshwater fish dishes, a vehicle for O. viverrini infection. The best approach to reducing CCA incidence is decreasing risk factors together with behavior alteration. Evaluation of CCA risk and its related factors are first needed for planning the prevention and control programs in the future. We therefore aimed to evaluate the CCA risk and explore its related factors among people in wetland communities of Ubon Ratchathani, Thailand. A cross-sectional study was conducted between July and August 2014. In total 906 participants, with informed consent, completed questionnaires. Overall risk of CCA was determined by multiplying odds ratios (ORs) of the risk factors for CCA from literature reviews. A mean score of 5.95 was applied as the cut-off point. Assessment of factors related to overall risk of CCA was accomplished using conditional logistic regression. Of all participants, 60.15% had a high level of the overall risk of CCA. Factors related to the overall risk of CCA were gender (p<0.001), marital status (p<0.001), perceived susceptibility (p=0.043) and prevention behavior for CCA (p<0.001). In conclusion, most participants in this community had a high level of overall risk of CCA. Therefore, integrated prevention and control programs continue to be urgently required.

• 한국어류학회지
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• 제20권1호
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• pp.7-12
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• 2008

돌마자 M. yaluensis 정자의 변형과정과 성숙한 정자의 미세해부학적 구조를 전자현미경으로 관찰하였다. 돌마자 정자형성과정에서 발생되는 편모는 핵의 한쪽 측면에서 발생하여 계속 신장되고 있어 잉어과 정자변형과정과 공통된 type 2로 분류할 수 있으며 성숙한 돌마자 정자의 두부 측면에 위치한 편모구조는 정자변형과정에서 핵의 회전이 일어나지 않았다는 것을 입증하고 있다. 두 중심립의 각도만큼 편모와 두부의 각도가 만들어지며 잉어과 어류 정자에서 두부와 편모는 두 중심립의 각도만큼 기울어져 있었다. 정자변형과정 중에 중심립 주변에서 관찰되는 골지체는 편모의 확장과 핵와 안으로 이동하는 데 필요한 물질을 제공하는 것으로 추정되며 정자변형과정 후기에는 관찰되지 않았다. 돌마자 정자의 세포질에서 나타나는 vesicles 구조는 중편보다 편모에서 많이 분포하며, 정자 편모의 vesicles은 기부에 주로 분포해 있으며 말단부에서는 나타나지 않는다. 중편세포질에 분포하는 미토콘드리아는 비대칭적으로 분포해 있으며, 미토콘드리아의 비대칭적 분포는 잉어류에서는 일부 종을 제외한 공통적인 특징인 것으로 사료된다.

• 한국환경생태학회지
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• 제31권1호
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• pp.54-61
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• 2017

습지생태계에 심각한 영향을 미치는 침입외래종 황소개구리(Rana catesbeiana)의 생태적 제어를 목적으로 효과적인 생물종을 선발하기 위하여 6종의 토종 포식성 어류와 6종의 조류를 이용하여 황소개구리의 올챙이와 아성체를 대상으로 포식률을 측정하였다. 본 실험에 사용한 어류 중 동자개(Pseudobagrus fulvidraco), 메기(Silurus asotus), 가물치(Channa argus) 3종은 황소개구리의 올챙이와 아성체를 모두 포식하였고, 그 중 전자를 더 선호하였다. 그러나 끄리(Opsariichthys uncirostris), 드렁허리(Monopterus albus), 뱀장어(Anguilla japonica) 3종은 황소개구리의 올챙이와 아성체를 전혀 섭식하지 않았다. 그리고 고니(Cygnus columbianus), 큰고니(Cygnus cygnus), 쇠오리(Anas crecca), 원앙(Aix galericulata), 홍머리오리(Anas penelope), 가창오리(Anas formosa) 등 실험에 사용한 6종의 조류 또한 황소개구리의 올챙이와 아성체를 전혀 먹지 않았다. 결과적으로, 국내 습지생태계에서 토종 물고기를 이용한 침입외래종 황소개구리의 제어가능성을 확인할 수 있었다.

• 해양환경안전학회:학술대회논문집
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• 해양환경안전학회 2017년도 추계학술발표회
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• pp.236-236
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• 2017

Effluent treated by an NaDCC injection method in Ballast water management system (BWMS) contains reactive chlorine species and disinfection by-products (DBPs). In this study, we conducted whole effluent toxicity (WET) testing and ecological risk assessment (ERA) to investigate its ecotoxicological effects on marine environment. WET testing was carried out for four marine pelagic and freshwater organisms, i.e., diatom Skeletonema costatum, Navicula pellicuosa, chlorophyta Dunaliella tertiolecta, Pseudokirchneriella subcapitata, rotifer Brachionus plicatilis, Brachionus calyciflorus and fish Cyprinodon variegatus, Pimephales promelas. The biological toxicity test revealed that algae was the only biota that showed apparent toxicity to the effluent; it showed no observed effect concentration (NOEC), lowest observable effect concentration (LOEC) and effect concentration of 50% (EC50) values of 25-50%, 50-100% and >100%, respectively, at three water condition, but did not show any significant toxicities on other biota. Meanwhile, chemical analysis revealed that the BWMS effluent contained total residual oxidants (TROs) below $0.03{\mu}g/L$ and a total of 25 DBPs such as bromate, volatile halogenated organic compounds (VOCs), halogenated acetonitriles (HANs), halogenated acetic acids (HAAs), chloropicrin and Isocyanuric acid. Based on ERA, the 25 DBPs were not considered to have persistency, bioaccumulation and toxicity (PBT) properties. The ratio of predicted environmental concentration (PEC) to predicted no effect concentration (PNEC) of the other DBPs did not exceed 1 for General harbor environment. However, four substances (Isocyanuric acid, Tribromomethane, Chloropicrin and Monochloroacetic acid) were exceed 1 for Nearship environment. But observed toxicity in the test water on algal growth inhibition would be mitigated by normal dilution factor of 5 applied for nearship exposure. Thus, our results of WET testing and ERA showed that the BWMS effluent treated by NaDCC injection method would have no adverse impacts on marine environment.