This study was conducted to examine the germination and growth during seedling period of Codonopsis lanceolata and Astragalus membranaceus as affected by different media. The seeds were sown in commercial medium (Tosilee), coir, rockwool, and urethane sponge. The seeds were germinated for 22 days in a closed-type plant production system, and seedlings were grown for 35 days after sowing in the venlo-type glasshouse located on Gyeongsang National University. Nutrient solution was supplied by the sub-irrigation as EC $1.0dS{\cdot}m^{-1}$ and pH 6.5 every second day. Final germination of Codonopsis lanceolata was the significantly highest in the coir and rockwool media as 68.5% and 67.9%, respectively. Mean daily germination was also the highest in the coir and rockwool media as 4.2 and 4.1, respectively. The germination rate of Astragalus membranaceus was not significantly difference in the media types. Plant height and leaf area of Codonopsis lanceolata were the significantly highest in the rockwool medium as 11.5 cm and $11.3cm^2$, respectively. Longest root length and fresh weight of root were the greatest in the coir medium as 5.8 cm and 0.07 g, respectively. Plant height and leaf area of Astragalus membranaceus were the significantly highest in the coir medium as 14.0 cm and $16.9cm^2$, respectively, and fresh and dry weights of root were the highest in the urethane sponge medium as 0.34 g and 0.03 g, respectively. Therefore, these result suggest that the rockwool and coir media were suitable for the germination and growth during seedling period of Codonopsis lanceolata and Astraglus membranaceus.
Kim, Tae-Gyeong;Lee, Woosung;Gang, Seongho;Kim, Jong-Sik;Chung, Chung-Wook
Journal of Life Science
/
v.29
no.1
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pp.76-83
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2019
In this study, the optimal growth and poly(3-hydroxybutyrate) (PHB) biosynthesis of Pseudomonas sp. EML2 were established using waste frying oil (WFO) as a cheap carbon source. The fatty acid composition of WFO and fresh frying oil (FFO) were analyzed by gas chromatography. The unsaturated and saturated fatty acid contents of the FFO were 82.6% and 14.9%, respectively. These contents changed in the WFO. The compositional change in the unsaturated fatty acid content in the WFO was due to a change in its chemical and physical properties resulting from heating, an oxidation reaction, and hydrolysis. The maximum dry cell weight (DCW) and PHB yield (g/l) of the isolated strain Pseudomonas sp. EML2 were confirmed under the following culture conditions: 30 g/l of WFO, 0.5 gl of $NH_4Cl$, pH 7, and $20^{\circ}C$. Based on this, the growth and PHB yield of Pseudomonas sp. EML2 were confirmed by 3 l jar fermentation. After the cells were cultured in 30 g/l of WFO for 96 h, the DCW, PHB content, and PHB yield of Pseudomonas sp. EML2 were 3.6 g/l, 73 wt%, and 2.6 g/l, respectively. Similar results were obtained using 30 g/l of FFO as a carbon source control. Using the FFO, the DCW, PHB content, and PHB yield were 3.4 g/l, 70 wt%, and 2.4 g/l, respectively. Pseudomonas sp. EML2 and WFO may be a new candidate and substrate, respectively, for industrial production of PHB.
Natural products has been occasionally used in place of growth regulators to control lateral root formation and growth of soybean sprouts. The study was done to measure the effects of treatment concentration of hard rubber tree (Eucommia ulmoides Oliver) leaf extract on lateral root formation, growth and morphological characters of the sprouts. Seeds of three cultivars, Pungsannamulkong, Sowonkong and Junjery, were imbibed for 5 hours into water, its 5 and 10% solutions immediately before 6 day culture. The cultured sprouts were classified into 4 categories to calculate their composition rates on the base of hypocotyl lengths; >7 cm (A),4 to 7 cm (B),<4 cm (C) and not germinated (D), and their morphological characters, fresh and dry weights were measured. Rate of A was decreased with increased concentrations, while that of C showed reverse result compared to A. Regardless of concentrations, the former rate was less in cv. Junjery than in cv. Pungsannamulkong and Sowonkong than in cv. Junjery although the latter rate was truly opposite to the former one. In 10% treatment concentration, lateral roots were less formed in cv. Junjery although nearly formed in cv. Pungsannamulkong and Sowonkong. In all the 3 cultivars, lateral roots per sprouts were decreased with increased treatment concentration. shorter and thicker sprout was observed in cv. Junjery than in the other cultivars, and in increased concentrations compared to relatively lower ones. Total fresh weight was the greatest in cv. Sowonkong due to mainly increment of its hypocotyl, but was reduced with increased concentration due to mainly decrement of hypocotyl in all the cultivars.
Park, Sangmin;Cho, Eunkyung;An, Jinhee;Yoon, Beomhee;Choi, Kiyoung;Choi, Eunyoung
Journal of Bio-Environment Control
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v.28
no.1
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pp.1-8
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2019
The study aimed to determine effects of light emitting diode (LED) and the ultraviolet radiation (UVA) light of plant factory on plant growth and ascorbic acid content of spinach (Spinacia oleracea cv. Shusiro). Plants were grown in a NFT (Nutrient Film Technique) system for 28 days after transplanting with fluorescent light (FL, control), LEDs and UVA (Blue+UVA (BUV), Red and Blue (R:B(2:1)) + UVA (RBUV), Red+UVA (RUV), White LED (W), Red and Blue (R:B(2:1)), Blue (B), Red (R)) under the same light intensity ($130{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and photoperiod (16/8h = day/night). All the light sources containing the R (R, RB, RUV, and RBUV) showed leaf epinasty symptom at 21 days after transplanting (DAT). Under the RUV treatment, the lengths of leaf and leaf petiole were significantly reduced and the leaf width was increased, lowering the leaf shape index, compared to the R treatment. Under the BUV, however, the lengths of leaf and leaf petiole were increased significantly, and the leaf number was increased compared to B. Under the RBUV treatment, the leaf length was significantly shorter than other treatments, while no significant difference between the RBUV and RB for the fresh and dry weights and leaf area. Dry weights at 28 days after transplanting were significantly higher in the R, RUV and BUV treatments than those in the W and FL. The leaf area was significantly higher under the BUV treatment. The ascorbic acid content of the 28 day-old spinach under the B was significantly higher, followed by the BUV, and significantly lower in FL and R. All the integrated data suggest that the BUV light seems to be the most suitable for growth and quality of hydroponically grown spinach in a plant factory.
In this study, we investigated the disease, insect damage, growth, and yield characteristics of sorghum and foxtail millet in an organic paddy-upland rotation system at Anseong-si Gyeonggi province, Korea, in 2016. Seven varieties of sorghum and foxtail millet are used in this study. A rotational upland field and continuously cropped upland field were compared in order to establish an optimum cultural method and to select the best sorghum and foxtail varieties for a paddy-upland rotation system. The incidence rate of major diseases was higher for sorghum than for foxtail millet. Moktaksusu (22.8%) had the highest incidence rate among sorghum varieties. Hwanggeumchal (7.0%) had the highest incidence rate among foxtail millet varieties. DS202 (89.3%) was the most severely damaged by Ostrinia furnacalis larva, which is the main pest of sorghum and maize. The weed numbers, and the weed fresh and dry weights were lower in the rotational upland field than in the continuously cropped upland field. Futhermore, the number of weed plants decreased by 42% and the dry weight of weeds decreased by 33% in the rotational upland field compared to the continuously cropped upland field. The culm length of sorghum and foxtail millet were 7.9 cm, and 3.4 cm longer, respectively, in the rotational upland field than in the continuously cropped upland field. The average yield of sorghum per 10 a increased by 90% in the rotational upland field compared to continuously cropped upland field. However, there was no significant difference between the rotational upland field and the continuously cropped upland field for foxtail millet. The most suitable varieties for a paddy-upland rotation system were judged to be Hwanggeumchal, Donganmae, and DS202 for sorghum, and Samdachal, Samdamae, Dahwangmae for foxtail millet.
We developed a new barley cultivar "Dami" (Hordeum vulgare L.) with the auricleless gene lig (al, li, aur-a). The characteristic of auricleless is a spontaneous mutant type which has known as a monogenic recessive gene. The plant with the gene has erect leaf blades because of no auricle. The cultivar was derived from a cross between 'BGS60' and 'Kangbori'. 'BGS60' has the auricleless gene (li), while 'Kangbori' showed a high biomass with winter hardiness and resistance to BaYMV (Barley Yellow Mosaic Virus). Subsequent generations were handled by the bulk method in a pedigree selection program. A promising line showed both high yield and lodging resistance in the yield trials at Iksan in 2003 to 2004, and designated as Iksan414. The line was subsequently evaluated for winter hardiness, earliness, and yield in the seven locations around Korea for three years from 2005 to 2007 and was designated as "Dami" and released. It has the growth habit of III, erect plant type, green leaf and stem similar to the check cultivar 'Sunwoo' Its heading date was April 30, and maturing date May 31 in paddy field conditions, which were similar to those of 'Sunwoo' respectively. The cultivar Dami was 97 cm in culm length, had 643 spikes per $m^2$ and higher leaf dry weight, and better adaptability to dense planting, winter hardiness, and resistance to BaYMV than the check cultivar did. The average forage yield of "Dami" was about 12 ton $ha^{-1}$ in dry matter (33 ton $ha^{-1}$ in fresh matter) in paddy field. "Dami" also showed 7.5% of crude protein content, 28.5% of ADF (Acid Detergent Fiber), 50.1% of NDF (Neutral Detergent Fiber), and 66.4% of TDN (Total Digestible Nutrients), including higher grade of silage quality for whole crop barley. This cultivar would be suitable for the area where the daily minimum temperature of January is above $-8^{\circ}C$ in Korean peninsula.
Kim, Ho Cheol;Heo, Buk Gu;Bae, Jong Hyang;Lee, Seung Yeob;Kang, Dong Hyeon;Ryu, Chan Seok;Kim, Dong Eok;Choi, I Jin;Ku, Yang Gyu
Korean Journal of Plant Resources
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v.29
no.4
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pp.495-503
/
2016
In the present study, we investigated the plant growth characteristics and biological activity of four asparagus cultivars grown using two cultural methods and tested the possibility of domestic open field. The number of shoots, buds, roots, shoot and root fresh and dry weights, and total dry weight of the four asparagus cultivars grown in a plastic house were higher than those of the same cultivars grown in an open field. Of the cultivars grown in the open field, Jersey Giant had greater shoot number than the other cultivars. In plastic house cultivation, the number of buds in Jersey Supreme was greater than the other cultivars. The total flavonoid content of the Jersey Giant was greater than the other cultivars, but cultural method was unaffected. The total polyphenol contents in asparagus cultivars grown in the plastic house were higher than those of cultivars grown in the open field. The total polyphenol content of the Jersey Giant grown the plastic house was significantly higher than those of other cultivars. Antioxidant activity such as catalase (CAT) and peroxidase (POX) did not differ significantly with cultural methods and among the cultivars. Ascorbate peroxidase (APX) activity of asparagus cultivars grown in the open field was higher than that of cultivars grown in the greenhouse; the highest APX activity was detected in UC157. Thus, greenhouse cultivation is expected to improve plant growth characteristics and biological activities of asparagus cultivars; each cultural method should be considered when selecting a suitable cultivar for high yield and high bioactive compound content.
In developing soil wetting agent using polyoxyethylene nonylphenyl ether (PNE) and polyoxyethylene castor oil (1:1; v/v), the effect of application rates on changes in concentration of PNE, initial wetting of peatmoss + perlite (7:3) medium, and growth of hot pepper (Capsicum annuum L. 'Knockwang') plug seedlings were investigated. The elevation of application rates of wetting agent increased the amount of water retained by the root media. The treatment of 2.5 $mL{\cdot}L^{-1}$ showed similar water retention to + control ($AquaGro^L$ 3.0 $mL{\cdot}L^{-1}$). Most of the liquid wetting agent (LWA) incorporated during the medium formulation leached out in the first and second irrigation, then it decreased gradually until 10 times in irrigation. In investigation of the influence of LWA on position of water infiltrating into root media, the vertical water movements in treatments of 0.5, 1.0, and 1.5 $mL{\cdot}L^{-1}$ were much faster than those in 0.0 $mL{\cdot}L^{-1}$ (-control), but relative speed of water movement decreased by the elevation in application rate of LWA to 2.0 or 2.5 $mL{\cdot}L^{-1}$. The evaporative water loss of root media that to contained various rate of LWA and irrigated to reach container capacity was the fastest in -control among the treatments and it delayed as the application rate of LWA was elevated. The plant height of 22.2 cm in 0.5 $mL{\cdot}L^{-1}$ and stem diameter of 3.26 mm in 1.0 $mL{\cdot}L^{-1}$ were the highest among the treatments tested. The treatment of 1.0 $mL{\cdot}L^{-1}$ also had the heaviest fresh and dry weights such among treatments tested as 3.08 g and 0.861 g per plant, respectively. The elevated application rate over than 1.5 $mL{\cdot}L^{-1}$ resulted in decreased seedling growth. The results mentioned above indicate that optimum application rate of LWA is 1.0 $mL{\cdot}L^{-1}$.
Aim of this study was to investigate the effects of different nutrient solutions and various light qualities generated by LED on the growth and glucosinolates contents of watercress (Nasturtium officinale) grown under hydroponics for 3 weeks. The seeds of watercress were sown on crushed rockwool media and raised them for two weeks. They were transplanted in a semi-DFT (deep flow technique) hydroponics system. A controlled-environment room was maintained at $20{\pm}1^{\circ}C$ and $16{\pm}1^{\circ}C$ temperatures and $65{\pm}10%$ and $75{\pm}10%$ relative humidity (day and night, respectively), with a provided photosynthetic photon flux density (PPFD) of $180{\pm}10{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and a photoperiod of 16/8h. To find out the best kinds of nutrient solutions for growing watercress, Otsuka House 1A (OTS), Horticultural Experiment Station in Korea (HES), and Netherland's Proefstaion voor Bloemisterij en Gasgroente (PBG) were adapted with initial EC of $1.0-1.3dS{\cdot}m^{-1}$ and pH of 6.2, irradiating PPFD with fluorescent lamps (Ex-1). Either monochromatic (W10 and R10) or mixed LEDs (R5B1, R3B1, R2B1G1, and W2B1G1) were irradiated with a differing ratio of each LED's PPFD to understanding light quality on the growth and glucosinolates contents of watercress (Ex-2). Although significant difference in the shoot growth of watercress was not found among three nutrient solutions treatments, but the root fresh weight increased by 13.7% and 55.1% in PBG and OTS compared to HES, respectively. OTS increased the gluconasturtiin content by 96% and 65% compared to PBG and HES. Compared with the white light (W10), the red light (R10) showed a 101.3% increase in the shoot length of watercress. Increasing blue light portion positively affected plant growth. The content of total glucosinolates in watercress was increased by 144.5% and 70% per unit dry weight in R3B1 treatment compared with R2B1G1 and W10 treatments, respectively. The growth and total glucosinolates contents of the watercress were highest under R3B1 among six light qualities.
Seo, Sung;Kim, Won-Ho;Kim, Jong-Geun;Choi, Gi-Jun;Kim, Ki-Yong;Cho, Won-Mo;Park, Beom-Young;Kim, Young-Hoon
Journal of The Korean Society of Grassland and Forage Science
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v.30
no.3
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pp.257-266
/
2010
This study was carried out to investigate the effect of feeding whole crop barley silage (WBS) on the growth performance, gain, feed requirement, carcass grade and beef quality of Hanwoo steers in 1999 to 2001. Twenty one steers were allocated, and divided into three treatment groups which fed rice straw only (control), WBS only, and fresh rice straw silage plus WBS during overall period ad libitum. Concentrates were fed at 1.5% level of live-body weight during growing stages, 4~12 months of age, at 1.8% level of live-body weight during early fattening stages, 13~18 months of age, and ad libitum after late fattening stages, 19~27months of age. The production yields of WBS were 17,135 kg as a silage, and 6,011 kg as a dry matter per ha, and the quality of WBS was 2~3 grade, while that of rice straw silage was 4grade. The daily gain with feeding of WBS (0.70 kg) during experimental period was higher than that of control group (0.65 kg), and the concentrate requirement (7.31 kg) per kg gain in WBS was lower than that of control (8.95 kg). The effects of gain and feed efficiency were very distinct during growing stage. i.e., the daily gain of WBS (0.84 kg) was increased by 65% compared to control (0.51 kg), and the concentrate requirement (4.16 kg) per kg gain in feeding of WBS was decreased by 35%, compared to control (6.39 kg). Meat quality with WBS was higher than that of control. Frequency rate of 1 and/or $1^+$ grade, and marbling score of feeding of WBS were 62.5% and 4.38, while those of control were 37.5% and 2.75, respectively. The results of the sensory evaluation showed that Hanwoo beef fed with WBS were better than that of control. In conclusion, feeding of WBS was desirable to improve daily gain, reduction of concentrates, feed efficiency and meat quality of Hanwoo steers.
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