• Biomedical Science Letters
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• v.6 no.1
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• pp.29-36
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• 2000

To investigate the effect of injection frequency of bromobenzene on the liver damage, bromobenzene (400 mg/kg, i.p.) was given daily to rats for six days. All experimental animals were sacrificed at 24 hours after the last injection. Morphological changes of the liver were observed under a light microscopic examination. Functional changes of the liver were evaluated by the measurement of alanine aminotransferase activity. To clarify the cause of discrepancy in liver damage, hepatic glutathione (GSH) content, glutathione S-transferase (GST) and aniline hydroxylase (AH) activities were determined. In the experiments of daily bromobenzene treatments, the sacrificed animals at six day (6 time-injected animals) showed slighter liver damage than those sacrificed at 3 day (3 time-injected ones), based on the liver morphological or functional findings; the decreasing ratio of GSH content and increasing ratio of liver GST and AH activities in the 6 time-injected group were higher than those in the 3 time-injected one.

• Biomedical Science Letters
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• v.6 no.2
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• pp.101-107
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• 2000

To evaluate the effect of intervals of bromobenzene treatment on the liver damage, the bromobenzene (400 mg/kg, i.p.) was given to rats at either one day or two days interval at three times. All the experimental animals were sacrificed at 24 hours after the last injection. Liver morphological changes were observed under a light microscopic examination and liver functional changes were determined by the measurement of alaine aminotransferase (ALT) activity and hepatic malondialdehyde (MDA) content. The experimental to examine the cause of liver damage were cytochrome P45O, glutathione (GSH) content and glutathione S-transferase (GST) activities. The results are summarized as follows; Based on the liver morphological and functional findings, the daily bromobenzene-treated rats (ED) showed the more severe liver damage than every other day bromobenzene-treated rats (EOD). The hepatic cytochrome P45O content was higher in EOD group than that in ED group. And the increasing rate of hepatic GST activity and decreasing rate of GSH content to the control were higher in EOD group than that in ED group. In conclusion, the treatment of bromobenzene intermittently to the rats may lead to more reduced liver injury compared with the continuously treated animals when both cases are treated with the same dose and frequency, and it may be caused by the enhancement of bromobenzene metabolism.