• 한국가축번식학회지
• /
• 제21권2호
• /
• pp.95-102
• /
• 1997

The studies on the carried out to investigate the effective concentration of cryoprotectant agents and sucrose by one-step straw method of bovine embryos. The follicular oocytes were cultured in TCM-199 medium containing 10 IU/ml PMSG(Sigma, USA), 10 IU/ml hCG(Sigma, USA), 1$\mu\textrm{g}$/ml $\beta$-estradiol(Sigma, USA) and 10% FCS for 24~48 hrs in incubator with 5% CO2 in air at 38.5$^{\circ}C$ and then matured oocytes were again cultured for 12~18 hrs with motile capacitated sperm by preincubation of heparin. The bovine embryos following dehydration by cryoprotective agents and various concentrations of sucrose were directly plunged into liquid nitrogen and thawed in 3$0^{\circ}C$ water. Survival and in vitro developmental rate was defined as devellpmental rate on in vitro culture or FDA-test. The results are smmarized as followes : 1. The high in vitro developmental rates of bovine frozen embryos after rapidly thawed in freezing medium was attained 2.0M glycerol, 2.0M DMSO, 1M or 2.0M propanediol. 2. The high in vitro developmental rates of bovine frozen embryos after rapidly thawed in freezing medium was obtained single cryoprotectant(6.7~17.4%) than mixed cryoprotectants(6.7~16.7%). 3. In vitro developmental rate of bovine embryos after rapid frozen-thawing in the freezing medium added 0.25M and 0.50M sucrose were higher cleavage rate than those of sucrose concentration of 0.75M and 1.00M. 4. The freezing methods on in vitro developemental rates of bovine embryos was attained slow freezing method(9.70~15.6%) higher than rapid freezing method(9.4~13.3%).

• 한국도로학회논문집
• /
• 제3권2호
• /
• pp.103-112
• /
• 2001

본 연구는 재생골재를 도로의 보조기층재료 및 포장용 콘크리트 골재로 사용하기 위하여 수행되었다. 우선 보조기층재료로서의 활용성 여부를 파악하기 위하여 실내다짐시험, CBR 시험, 평판재하시험을 수행하였으며, 콘크리트용 골재로의 활용성을 보기 위하여 재생골재 첨가비율을 0, 20, 40, 60, 80%로 하여 설계기준강도 $280kgf/cm^2$인 표층용 콘크리트를 제조하였다. 제조된 콘크리트로 굳지 않은 콘크리트 성질과 28일 양생 후 강도시험과 동결 융해에 따른 내구성 시험을 통해 폐콘크리트 재생골재의 활용성을 도로포장재료 측면에서 검토하였다. 실험결과 재생골재는 보조기층재료로서의 사용이 충분히 가능하며 표층용 콘크리트 골재로서 재생골재 첨가비율 40%까지 활용이 가능함을 알 수 있었다.

• 한국지반공학회:학술대회논문집
• /
• 한국지반공학회 1995년도 연약지반 안정처리 시공.관리 세미나
• /
• pp.88-116
• /
• 1995

The applicability of marine clay and banking material as landfill liner and supporting layer was examined, where additives (cement, lime, Mg0, bentonite and ESCA) were mixed with these soils. The mixtures were tested for the unconfined compressive strength, bending strength, permeability, and better results than existing solidifying agents were obtained. The freezing-thawing test is under way.

• 한국콘크리트학회:학술대회논문집
• /
• 한국콘크리트학회 1990년도 가을 학술발표회 논문집
• /
• pp.163-168
• /
• 1990

This study shows both through experiment and based on theory the reinforcement effectiveness when using FRP(Fiber Reinforced Plastics) as a means of reinforcing the concrete of the deteriorated concrete. Non-deteriorated concrete and deteriorated concrete which is deteriorated by freezing and thawing are made three type specimens (non-reinforced) concrete beam, one layer FRP reinforced concrete beam, two layer FRP reinforced concrete beam) for this purpose. Bending strength and cracking load ratio is measured by bending test.

• 한국콘크리트학회:학술대회논문집
• /
• 한국콘크리트학회 1999년도 학회창립 10주년 기념 1999년도 가을 학술발표회 논문집
• /
• pp.263-266
• /
• 1999

This study aims to make high performance concrete for normal strength using crushed stone fines to control high strength of the high performance concrete. According to the experimental results, when crushed stone fines are increased every 10%, 15% of compressive strength is decreased, and 5% of drying shrinkage is increased, compared to normal high performance concrete. Also, high performance concrete has been evaluated to have good durability factor more than 100% in the 480cycle of freezing and thawing test, without regard to using AE and crushed stone fines.

• 한국콘크리트학회:학술대회논문집
• /
• 한국콘크리트학회 1998년도 봄 학술발표회 논문집(I)
• /
• pp.129-136
• /
• 1998

In this study, lightweight aggregates were developed to see the possible application as a structural uses. For the evaluation purpose, several testings were conducted to compare the physical characteristics between the controlled lightweight aggregates and other lightweight aggregates purchased from different sources. The tests included property changes of fresh concrete and strength characteristics of hardened concrete for both normal and high strength ranges. In addition, a experiment was performed to analyze the freezing and thawing resistance of new lightweight aggregate concrete against other lightweight aggregate concrete against other lightweight aggregate concretes with some experimental parameters such as lightweight aggregates, curing conditions, and water-cement ratio. The test showed that the new lightweight aggregate could be used structural components. Continuous study will be planned for future evaluations.

• 한국구조물진단유지관리공학회 논문집
• /
• 제19권3호
• /
• pp.30-38
• /
• 2015

적설한랭지의 경우, 동절기의 동해 및 적설에 의한 교통통행의 원활을 기하기 위하여 제설제를 사용한다. 이와 같은 제설염의 사용은 동결융해 및 염화물에 의해 콘크리트의 급속한 스케일링 피해를 야기시킨다. 이에 대한 피해로 인하여 콘크리트 포장은 콘크리트의 피복두께 감소에 따른 조기 열화 및 사용자의 안정성 등 여러 가지 문제를 발생시킨다. 이에 따라 국외적으로 이와 같은 현상에 대하여 평가를 하기 위해 다양한 시험 및 평가 방법이 제시되고 있다. 하지만 국내에서는 아직 평가방법에 대한 규정은 없으며, 관련 연구 또한 미미한 수준이다. 따라서 본 논문에서는 각 기관 및 나라에서 제시되고 있는 평가방법을 조사하고, 각각의 규정에 따라 실험을 수행하여 결과를 도출하여 비교 분석하였다. 나아가 이와 같은 실험방법과 실험결과의 고찰을 통하여 국내현장에 맞는 실험법을 제안하고자 한다.

• 한국지반환경공학회 논문집
• /
• 제16권1호
• /
• pp.37-43
• /
• 2015

우리나라는 사계절이 뚜렷하며 여름에는 고온다습한 무더운 날씨를 보이고 겨울에는 혹한의 날씨가 지속된다. 이러한 계절적 특성을 고려하여 국내 토공 분야에서는 많은 연구들이 있었으나 대부분의 연구가 지반의 동결 융해에 초점을 맞추어 이루어졌다. 이러한 연구들은 시공 당시의 기온에 따른 지반특성을 반영하지 못하였으며 상온에서 다져진 지반을 대상으로 실시되었다. 그러나 동절기 다짐의 경우 상온에서의 다짐과는 다른 지반상태를 나타낼 것으로 예상되며, 그에 따른 동결 융해로 인한 강도 및 변형 특성 또한 다르게 나타날 것으로 판단되나 이를 고려한 연구는 아직까지 전무한 상황이다. 따라서 본 연구에서는 $-3^{\circ}C$와 $-8^{\circ}C$에서 주문진 표준사에 카올리나이트를 0%, 5%, 10%, 15%의 중량비로 혼합하여 다짐시험을 실시하였으며, 상온($18^{\circ}C$)에서의 다짐곡선과 비교하여 온도 및 세립분 함량에 따른 다짐 효과를 살펴보았다. 시험 결과, 동결 온도에서 다짐 시 최대건조단위중량이 감소하는 경향을 보였으며, $-8^{\circ}C$ 이하에서 다짐 시공 시 시방서의 다짐기준을 만족하지 못하는 것으로 나타났다.

• 한국동물생명공학회지
• /
• 제34권3호
• /
• pp.247-252
• /
• 2019

We observed MMPs expression in all sperm groups, with pro-MMP showing lower expression than active MMPs. According to the results from each freezing extender, the sperm membrane integrity (HOST: Hypoosmotic Swelling Test) analysis in TCGGD (Tris 250 mM, Citric acid 88 mM, Glucose 47 mM, Glycerol 3%, Dimethylsulpoxide 3.5 M) is 59.8 ± 0.7, TCGSD (Tris 250 mM, Citric acid 88 mM, Glucose 47 mM, Sucrose 0.1 M, Dimethylsulpoxide 3.5 M) is 59.3 ± 0.5 were significantly higher (p < 0.05) among the experimental groups. And MMPs analysis result, we observed MMPs expression in all sperm groups, with pro-MMP showing lower expression than active MMPs. The expression of active MMP-2 was the highest in sperms frozen in TCGSD and TCGD (Tris 250 mM, Citric acid 88 mM, Glucose 47 mM, Dimethylsulpoxide 3.5 M), Meanwhile, sperms from the TCGGD and TCGED (Tris 250 mM, Citric acid 88 mM, Glucose 47 mM, Ethylene glycol 3%, Dimethylsulpoxide 3.5 M) group showed lower level of active MMP-2 expression. Together, these results indicate that adding glycerol or sucrose to the sperm freezing buffer would not only suppress MMPs expression but also minimize DNA fragmentation, providing a mean to improve the success rate in the in vitro manipulation of rabbit sperms. Therefore, these results suggest that TCGGD or TCGSD extender method for freezing-thawing of rabbit sperm increased the viability after thawing.

• 한국콘크리트학회:학술대회논문집
• /
• 한국콘크리트학회 2000년도 봄 학술발표회 논문집
• /
• pp.142-147
• /
• 2000

Durability characteristics of controlled low strength material(flowable fill) with high volume fly ash content was examined. The mix proportions used for flowable fill are selected to obtain low-strength material in the 10 to 15kgf/㎥ range. The optimized flowable fill was consisted of 60kgf/㎥ cement content, 280kgf/㎥ fly ash content, 1400kgf/㎥sand content, and 320kgf/㎥water content. Subsequently, durability tests including permeability warm water immersion, repeated wetting & drying, freezing & thawing for high volume fly ash-flowable fill are conducted The test results indicated that flowable fill has has acceptable durability characteristics.