• Title/Summary/Keyword: Freezing Method

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Measurement of Size Distributions of Submicron Electrosprays Using a Freezing Method and an Image Processing Technique (냉각법 및 영상 처리기법을 이용한 서브마이크론 정전분무 액적의 크기분포 측정)

  • Gu, Bon-Gi;Kim, Sang-Su;Kim, Yu-Dong;Lee, Sang-Yong
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.25 no.10
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    • pp.1400-1407
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    • 2001
  • The size distributions of electrospray droplets from the Taylor cone in cone-jet mode are directly measured by using a freezing method and a transmission electron microscope (TEM) image processing technique. These results are compared with the data obtained by an aerodynamic size spectrometer (TSI Aerosizer DSP). The use of glycerol seeded with NaI and a freezing method make it possible to sample droplets with their original sizes preserved. Since pictures of droplets are taken with TEM with very low vapor pressure of the solution, evaporation is suppressed by freezing. For liquid flow rates below 1 nl/sec, the measured droplet diameters by the TEM image processing technique and the aerosizer are in the range of 0.25 to 0.32 m add 0.3B to 0.40m, respectively. Comparing the TEM data with the aerosizer measurements, it has been revealed that the TEM image processing technique can afford more accurate values of droplet size distributions in the submicron range of 0.1 to 0.4m.

Establishment of Freezing Conditions for Improving Cryosurvival in Miniature Pig Spermatozoa (미니돼지 정액의 동결-융해 후 생존율 향상을 위한 동결 조건 확립)

  • Lee, Yong-Seung;Yoo, Han-Jun;Cheong, Hee-Tae;Yang, Boo-Keun;Woo, Jea-Seok;Park, Choon-Keun
    • Reproductive and Developmental Biology
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    • v.35 no.3
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    • pp.329-334
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    • 2011
  • This study was conducted to establish a freezing method of miniature pig spermatozoa. The semen 더aculated from PWG M-type miniature pig was collected by gloved-hand method. The semen was diluted with same volume extender (m-Modena B). The frozen solution used frozen solution of four different (LEY, TCG, BF-5 and m-Modena+egg yolk) for find optimal frozen solution in miniature pig sperm. The diluted semen for frozen rate assay was added to LEY solution (solution I: 11% lactose+egg yolk; solution II: solution I+glycerol+OEP), and frozen depending on freezing rate by the three different freezing methods (A: until $5^{\circ}C$ for 1 hrs, holding at $-102^{\circ}C$ for 10 min; B: until $5^{\circ}C$ for 2 hrs, holding at $-102^{\circ}C$ for 10 min; C: until $5^{\circ}C$ for 3 hrs, holding at -80 and $-102^{\circ}C$ for 10 min). Semen cooled until $5^{\circ}C$ was added with glycerol 1, 3 and 5%, and take a equilibrium time for 0, 10 and 30min. Frozen-thawed sperm were evaluated for viability, acrosomal status and morphological abnormality. The results of frozen-thawed sperm ability by frozen solution, viability was higher in LEY solution compared to other three different frozen solution. AR pattern of LEY solution were lower than other three different frozen solution. The results of freezing rate, viability was higher in B method compared to other methods (p<0.05). Acrosomal statute was intacted in A and B methods than C method. The experiment for glycerol condition was showed that sperm viability was higher in extender with 1% and 3% glycerol and equilibrium time of 0 min. The acrosome damage was lower in extender with 1% glycerol and equilibrium time of 10 min than other conditions. In conclusion, the optimal conditions for cryopreservation of miniature pig spermatozoa obtained in LEY frozen solution, cooling rate of 1~2 hrs, 1~3% glycerol concentrations and glycerol equilibrium time of 0~10 min.

Studies on the Freezing Time Prediction and Factors Influencing Freezing Time Prediction (식품의 동결시간 예측 및 동결시간에 영향을 미치는 요인에 관한 연구)

  • Kong, Jai-Yul;Jeong, Jin-Woong;Kim, Min-Young
    • Korean Journal of Food Science and Technology
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    • v.20 no.6
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    • pp.827-833
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    • 1988
  • The objectives of this investigation were to develop an improved analytical method and to review with respect to experimental parameters and thermo-physical properties influencing the freezing time prediction. The results indicate that the relationship between freezing time and product size is dependent on the surface heat transfer coefficient. As the magnitude of surface heat transfer coefficient decreases, the influence of product size on freezing time becomes more profound. But the freezing time does decrease slightly as the coefficients are increased to values greater than 150 $w/m^2^{\circ}C$. In addition, influence of thermo-physical properties on the freezing time prediction shown generally density, water content, specific heat and thermal conductivity, in order of % difference. Multiple linear regression equation for freezing time prediction were obtained with respect to 4 different food materials with varying thickness.

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An Experimental Study on the Resistance of Concrete Included Rice Husk Ash Against Rapid Freezing and Thawing (왕겨재를 혼입한 콘크리트의 동결융해 저항성에 관한 실험적 연구)

  • 이준구;박광수;이응찬;김한중
    • Proceedings of the Korean Society of Agricultural Engineers Conference
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    • 1999.10c
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    • pp.294-300
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    • 1999
  • After researching the physical properties of the concrete included Rice Husk Ash(RHA concrete) and workability of fresh concrete admixed RHA, we have tested durability of RHA-concrete against freeaing and thawing in the winter using rapid freezing and thawing test method(KS F 2456) . There aretwo hypotheses to explain the failure mechanism of a freezing and thawing action. First, the hydraulic pressure in the pores of freezing concrete make an internal stress of concrete structures outbreaking micro crack in the face of concrete, Second, Frost action causing damage to cement paste repeatedly come from soil frost action, freezing water in the capillaries. Initial Relative Dynamic Modulus of Elasticity (DME) was biggest in cae of unit binder weight 600kgf/㎥ and relative dynamic modulus of elasticity increased until 300cycles. In general , initial relative DME was proportional to unit binder weight . Relative DME was decreased in proportion to unit binder weight in the case of 300, 400, 500kgf/㎥ , but relative DME fo the others remained more than 90% until 300 cycles. It was not good effect of intermixed RHA to concrete in case of below unit binder weight 300kgf/㎥ and the resistance of freezing and thawing was not good either.

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Dertermination of Alkylphenols, Chlorophenols and Bisphenol A in Various Samples by Freezing Filtration and GC/MS-SIM

  • Kim, Hyub
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.68.1-68.1
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    • 2003
  • A method for determination 11 endocrine disrupting chemicals of phenols in various samples was deloped. The alkylphenols, chlorophenols and bisphenol A were determined by gas chromatography/mass spectrometry-selected ion monitoring (GC/MS-SIM) followed by two work-up methods for comparison; isobutoxycarbonyl (isoBOC) derivatization method and tert-butyldimethylsilyl (TBDMS) derivatization method. Eleven endocrine disrupting chemicals (EDCs) of phenols in biological samples were extracted with acetonitrile and then acetonitrile layer was refrigerated at -60$^{\circ}C$ for 2 hours (freezing filtration). (omitted)

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Changes in Quality during Frozen Storage of Meat with Thermal Equalized Freezing (균온처리 동결에 의한 식육의 저장중 품질변화)

  • Jeong, Jin-Woong;Lee, Ho-Jun;Park, Noh-Hyun
    • Korean Journal of Food Science and Technology
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    • v.31 no.3
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    • pp.688-696
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    • 1999
  • Changes in quality during frozen storage of meat with thermal equalized freezing and various freezing methods were investigated. When beef were frozen at freezing rate of $0.39{\sim}0.66\;cm/h$, average diameter of ice crystal were about $30{\sim}50\;{\mu}m$ and showed broken tissues or irregular cracks. At freezing velocity of $1.14{\sim}2.26\;cm/h$, ice crystals of about $10{\sim}30\;{\mu}m$ was formed mainly inside or between fiber and slight destruction of tissues was occurred. The average diameter (D) of the ice crystals were related to the characteristic freezing time $(t_c)$ by the equation: $D({\mu}m)=4.089+26.88logt_c\;(r^2=0.913)$. Beef with still-air freezing showed higher drip loss than methods of immersion and thermal equalized freezing. Also, drip loss of pork was relatively lower than beef and showed highest value to 7.39% during storage on 40 days at air-blast freezing method. No apparent change of pH during storage of frozen beef and pork by freezing methods were detected. However, least changes for sample with thermal equalized freezing was found compare to sample with still-air and air blast freezing in VBN and TBA value. The fluctuation of frozen storage temperature did not cause noticeable changes on pH and water content. However, drip loss, VBN and TBA values were increased slowly as frequency of fluctuation increased.

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Experimental and Numerical Study on Hydro-thermal Behaviour of Artificial Freezing System with Water Flow (물의 흐름을 고려한 인공동결 시스템의 열-수리 거동 연구)

  • Jin, Hyunwoo;Lee, Jangguen;Ryu, Byung Hyun;Go, Gyu-Hyun
    • Journal of the Korean Geotechnical Society
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    • v.36 no.12
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    • pp.17-25
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    • 2020
  • The artificial ground freezing method is a ground amelioration technology that does not have a permanent effect on the ground. One of the key factors that determine the efficiency and design criteria of the artificial ground freezing is the groundwater flow. Therefore, in order to accurately evaluate the behavior of the artificial ground freezing, studies on the effect of water flow on the formation of ice walls must be preceded. In this paper, experimental and numerical analyses were conducted using only pure water to maximize the effect of water flow on the formation of ice walls. A hydro-thermal coupled model for freezing behavior was proposed and the accuracy of the model was verified. Through the numerical and experimental studies, the flow rate dominates not only the formation time but also the shape of the ice wall. In addition, this study proposes a method to indirectly predict the ice wall formation time, which is expected to be highly useful for a practical application where it is difficult to visually identify ice walls.

Stable Anisotropic Freezing Modeling Technique Using the Interaction between IISPH Fluids and Ice Particles (안정적이고 이방성한 빙결 모델링을 위한 암시적 비압축성 유체와 얼음 입자간의 상호작용 기법)

  • Kim, Jong-Hyun
    • Journal of the Korea Computer Graphics Society
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    • v.26 no.5
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    • pp.1-13
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    • 2020
  • In this paper, we propose a new method to stable simulation the directional ice shape by coupling of freezing solver and viscous water flow. The proposed ice modeling framework considers viscous fluid flow in the direction of ice growth, which is important in freezing simulation. The water simulation solution uses the method of applying a new viscous technique to the IISPH(Implicit incompressible SPH) simulation, and the ice direction and the glaze effect use the proposed anisotropic freezing solution. The condition in which water particles change state to ice particles is calculated as a function of humidity and new energy with water flow. Humidity approximates a virtual water film on the surface of the object, and fluid flow is incorporated into our anisotropic freezing solution to guide the growth direction of ice. As a result, the results of the glaze and directional freezing simulations are shown stably according to the flow direction of viscous water.

Experimental and numerical investigation of closure time during artificial ground freezing with vertical flow

  • Jin, Hyunwoo;Go, Gyu-Hyun;Ryu, Byung Hyun;Lee, Jangguen
    • Geomechanics and Engineering
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    • v.27 no.5
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    • pp.433-445
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    • 2021
  • Artificial ground freezing (AGF) is a commonly used geotechnical support technique that can be applied in any soil type and has low environmental impact. Experimental and numerical investigations have been conducted to optimize AGF for application in diverse scenarios. Precise simulation of groundwater flow is crucial to improving the reliability these investigations' results. Previous experimental research has mostly considered horizontal seepage flow, which does not allow accurate calculation of the groundwater flow velocity due to spatial variation of the piezometric head. This study adopted vertical seepage flow-which can maintain a constant cross-sectional area-to eliminate the limitations of using horizontal seepage flow. The closure time is a measure of the time taken for an impermeable layer to begin to form, this being the time for a frozen soil-ice wall to start forming adjacent to the freeze pipes; this is of great importance to applied AGF. This study reports verification of the reliability of our experimental apparatus and measurement system using only water, because temperature data could be measured while freezing was observed visually. Subsequent experimental AFG tests with saturated sandy soil were also performed. From the experimental results, a method of estimating closure time is proposed using the inflection point in the thermal conductivity difference between pore water and pore ice. It is expected that this estimation method will be highly applicable in the field. A further parametric study assessed factors influencing the closure time using a two-dimensional coupled thermo-hydraulic numerical analysis model that can simulate the AGF of saturated sandy soil considering groundwater flow. It shows that the closure time is affected by factors such as hydraulic gradient, unfrozen permeability, particle thermal conductivity, and freezing temperature. Among these factors, changes in the unfrozen permeability and particle thermal conductivity have less effect on the formation of frozen soil-ice walls when the freezing temperature is sufficiently low.

Effect of ultrasonic waves on anti-freezing for plate-type heat exchanger (평판형 열교환기에서의 제빙억제를 위한 초음파의 영향)

  • Cho, Ki-Ryang;Choi, Kwang-Il;Kim, On;Park, Ki-won
    • Journal of Advanced Marine Engineering and Technology
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    • v.41 no.3
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    • pp.176-181
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    • 2017
  • This study attempted to examine a method of anti-freezing on a plate-type heat exchanger in a low-temperature environment. Freezing condition was observed after ultrasonic waves were generated. Data were recorded to determine the optimal conditions for freezing. Ethylene glycol, which is commonly used in antifreeze formulations, was used as the brine, and the temperature was varied between -8 and $-16^{\circ}C$. The water for freezing provided by the thickness of 1-3 mm. In addition, experiments were conducted by adjusting the output to identify the changes that occurred due to the incidence of ultrasonic energy. The results of the anti-freezing effect were brine temperature, freezing thickness, and frequency band of ultrasonic waves.