• Geomechanics and Engineering
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• v.30 no.4
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• pp.393-399
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• 2022

The Segregation Potential (SP) is one of the most widely used predictors of frost heave in cold regions. Laboratory step-freezing tests determining a representative SP at the onset of the formation of the last ice lens (near the thermal steady state condition) can predict susceptibility to frost heave. Previous work has proposed empirical semi-log fitting for determination of the representative SP and applied it to several fine-grained soils, but considering only frost-susceptible soils. The presence of fines in coarse-grained soil affects frost susceptibility. Therefore, it is required to evaluate the applicability of the empirical semi-log fitting for both frost-susceptible and non-frost-susceptible soils with fines content. This paper reports laboratory frost heave tests for fines contents of 5%-70%. The frost susceptibility of soil mixtures composed of sand and silt was classified by the representative SP, and the suitability of the empirical semi-log fitting method was assessed. Combining semi-log fitting with simple laboratory frost heave testing using a temperature-controllable cell is shown to be suitable for both frost-susceptible and non-frost-susceptible soils. In addition, initially non-frost-susceptible soil became frost susceptible at a 10%-20% weight fraction of fines. This threshold fines content matched well with transitions in the engineering characteristics of both the unfrozen and frozen soil mixtures.

• Korean Journal of Animal Reproduction
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• v.17 no.4
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• pp.311-323
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• 1994

This study was conducted to observe seasonal and individual changes in semen characteristics and sperm freezability, and sperm penetration into zona-free hamster eggs in Korean native goats. Buck response and change in semen characteristics to electrical stimulations was evaluated for four seasons throughout 2 years and percentage of motile sperm and normal apical ridge acrosome was investigated after equilibration and thawing for 4 seasons with 5 bucks. Sperm penetration rate was evaluated for 4 bucks. 1. Probe insertion at depth of 7cm and repeated stimulation for 3 sec was more effective(P<0.05) in buck response and semen collection than those of other conditions. 2. Semen characteristics from electrojaculation was signficantly(P<0.005) higher in spring and fall for semen volume, in spring and summer for sperm concentration and in fall for sperm motility than those in other seasons, respectively. However, there were no differences in total sperm among seasons. 3. Buck response to electrical stimulation showed significant difference(P<0.05) among individuals in all 3 seasons except winter. Significant individual difference in semen volume was only in spring and summer, but there was no indivudual difference in sperm concentration and total sperm in all season. 4. Washing of semen before freezing treatment was greatly(P<0.05) beneficial to sperm motility after thawing, no matter whether ejaculates exhibit egg yolk coagulation or not. 5. Sperm motility after glycerol equilibration was significantly(P<0.05) low in summer semen and motility after thawing was greatly(P<0.05) higher in winter semen than in other seasons. Freezability of unwashed sperm was significantly difference among bucks, but a yearly freezability of washed sperm after chilling and thawing were no differences among bucks and percentage of normal apical ridge acrosome were not different among seasons and bucks. 6. There was no significant difference in sperm motility after thawing between egg yolk levels in summer, although 20% level gave more higher motility than 5% level. 7. In summer, 3.2% glycerol and 3-h equilibration gave greatest percentage(P<0.05) of sperm motility and normal apical ridge acrosome after thawing. 8. Sperm penetration rate into zona-free hamster eggs was not different between bucks and seasons. Overall, it is concluded that to obtain maximum sperm output and successive semen freezing by electrojaculation method, buck selection with good response in all season could be basically considered and that seasonal effect on sperm freezability was more greater than that of individual bucks.

• Korean Journal of Animal Reproduction
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• v.26 no.3
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• pp.245-252
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• 2002

This study was to evaluate the in vitro survival of bovine enucleated MII (eMII) oocytes according to minimum volume cooling (MVC) freezing method and activation timing, and their in vitro development after somatic cell nuclear transfer (SONT). in vitro matured bovine oocytes for 20 h were stained with 5 $\mu\textrm{g}$/$m\ell$ Hoechst, and their 1st polar body and MII plate were removed by enucleation micropipette under UV filter. Also, eMII oocytes were subjected to activation after (group II) and before (group III) vitrification in 5 ${\mu}{\textrm}{m}$ ionomycin added CRlaa medium for 5 min. For vitrification, eMll oocytes were pretreated with EG10 for 5 min, exposed to EG30 for 30 sec and then directly plunged into L$N_2$. Thawing was taken by 4-step procedures at 37$^{\circ}C$. Survived eMII oocytes were subjected to SONT with cultured adult bovine ear cells. Reconstructed oocytes were cultured in 10 $\mu\textrm{g}$/$m\ell$ of cycloheximide and 2.5 $\mu\textrm{g}$/$m\ell$ of cytochalasin D added CRlaa medium for 1 h, and then in 10 $\mu\textrm{g}$/$m\ell$ of cycloheximide added CRlaa medium for 4 h. Subsequently, the reconstructed oocytes were incubated for 2 days and cleaved embryos were further cultured on cumulus-cell monolayer drop in CRlaa medium for 6 days. Survival rates of bovine vitrified-thawed eMII oocytes in group II (activation after vitrification and thawing) and III (activation before vitrification) were 81.0% and 84.9%, respectively. Fusion rates of cytoplasts and oocytes in group II and III were 69.0% and 70.0%, respectively, and their results were not different with non-frozen NT group (control, 75.2%). Although their cleaved rates (53.4% and 58.4%) were not different, cytoplasmic fragment rate in group II (32.8%) was significantly higher than that in group III (15.6%)(P<0.05). Also, subsequent development rate into >morula in group II (8.6%) was low than that in group III(15.6%). However, in vitro development rate in group III was not different with that in control (24.8%). This result suggested that MVC method was appropriate freezing method for the bovine eMII oocytes and vitrified eMII oocytes after pre-activation could support in vitro embryonic development after SONT as equally well as fresh oocytes.

• Food Science of Animal Resources
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• v.24 no.2
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• pp.151-155
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• 2004

This study was performed to develop the method of differentiation fresh and frozen meat by using the measurement of mitochondrial malate dehydrogenase. The principle of this experiment is based on the fact the enzyme proteins associated with mitochondria membrane could be released by freezing. The methods were studied by measurements of protein concentration of meat press juice, WHC (water-holding capacity), drip loss and mitochondrial malate dehydrogenase enzyme activity. Samples were stored at 4$^{\circ}C$ and -18$^{\circ}C$ during storage period, respectively. Protein concentration of meat press juice was ranged from 8.5 mg/mL to 12.7 mg/mL and increased by freezing below at -18$^{\circ}C$(p<0.05). The WHC was not significantly different between fresh meat and frozen chicken meat (p＞0.05). The amount of drip loss of fresh and frozen chicken meat at 4$^{\circ}C$ and -18$^{\circ}C$ was not significantly different (p＞0.05). Mitochondrial malate dehydrogenase activity of frozen meat (-18$^{\circ}C$) was significantly higher (p＜0.05) than that of fresh meat. Also, enzyme activity of frozen meat was maintained at the same level after 3 minutes reaction. But fresh meat had not this reaction. From these results, it suggests that mitochondrial malate dehydrogenase can be used as a promising enzyme to differentiate between fresh and frozen meat.

• Journal of the Korea Concrete Institute
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• v.20 no.5
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• pp.653-660
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• 2008

The purpose of this study is to analyze the optimal mix design of the sound absorption material that is made from perlite and various binder systems for noise barrier wall. The polymer cement slurry which is made from two types of polymer dispersions, and silicone type inorganic material are used as binder. The test specimens are prepared with various polymer cement ratios, binder ratios, and tested for strengths, freezing and thawing and sound absorption performance by the tube and the reverberation room methods. From the test results, the difference of sound absorption coefficient by the tube method is a little recognized, however, noise reduction coefficient (NRC) of test specimens bound by the polymer cement slurry is in the ranges of 0.48 to 0.51. They are a little higher than those bound by cement only, and are lower values than recommended value of 0.7 by the Ministry of Environment. However, the sound absorption coefficient of test specimens at low frequency range of 250 to 500 Hz by reverberation room method shows very high values as 0.84 to 1.00, and 0.57 to 0.77 at the high frequency. The test specimens with polymer cement slurry binder have a good balance between performance and cost, and have proper properties in strengths, freezing and thawing resistance as sound absorption material for noise barrier wall. It is apparent that the good sound absorption material can be produced according to the optimum mix design that is recommended from this study.

• Clinical and Experimental Reproductive Medicine
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• v.30 no.3
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• pp.241-248
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• 2003

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

• International Journal of Highway Engineering
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• v.7 no.4 s.26
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• pp.113-123
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• 2005

In this study, calcium chloride$(CaCl_2)$, sodium chloride (NaCl), organic acids-containing deicer(NS 40, NS 100), mixed deicier$(NaCl\;70%+CaCl_2\;30%,\;NS\;40\;70%+CaCl_2\;30%,\;NaCl\;70%+NS\;40\;30%,\;NS\;40\;70%+NaCl\;30%)$ is investigated based on the laboratory test for deicing performance, freez-thaw resistance of concrete, and corrosion rate of metal. Test items for deicing performance were ice melting and ice penetration, freezing point depressions and eutectic point, pH, thermal properties for selected deicing chemicals as well as their compatibility with concrete and metal were experimentally investigated. As a test results, in case of the use chloride-containing deicier in area that concrete structures has subjected to freez-thaw reaction in winter season, it showed that mixed deicing chemicals with optimum ratio has desirable method than use one deicing chemicals when is consider to deicing performance and effects, corrosion of steel materials, and freez-thaw resistance of concrete. When use various deicing chemicals mixed, NS40(70%)+calcium chloride(30%) showed the best effective method.

• Journal of the Korean earth science society
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• v.36 no.6
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• pp.501-511
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• 2015

This study suggests a method of alleviating global warming by the increase of the Earth surface albedo through Artificial Sea ice Increasing (ASI) over the Available Freezing Areas (AFA). The method is developed based on the fact that the large sea surface area in or near the Arctic and the Antarctic has no ice even though both water and air temperatures are below zero and the artificial sea ice generation is thus available. The mean energy of $0.85Wm^{-2}$, which was suspected of adding to the earth by the global warming effect was calculated to offset at once when the sea ice area about $4.09{\times}10^6km^2$ was additionally increased. In addition, three techniques for producing ice plates on the sea surface (using ships, installation apparatus, and floating matter such as Green Cell Foam) for ASI were proposed. According to the result of simple analysis using the energy balance model, when ASI was maximally operated only for 3 months (September, October, and November) over AFA, it is expected that the annual mean temperature of earth surface would be decreased about $0.11^{\circ}C$ in the following year. On the other hand, in case of generating the artificial sea ice in all four seasons, a risk of triggering snowball earth was detected.

• Journal of the Korean GEO-environmental Society
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• v.21 no.3
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• pp.5-11
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• 2020

Recently, ground pits that have been occurring frequently in urban areas are hindering traffic flow and causing property damages and loss of human life, acting as factors that are threatening the safety of citizens. Therefore, sunken ground must be quickly restored and provisions must be made for additional damage but current domestic detailed standards regarding ground pits and accurate definitions regarding causes and measures to be taken for reoccurrences are lacking. Restoration methods of sunken ground include backfilling by reusing sunken soil or other fill material and paving the road and while this is the most often used method, this only prevents ground from sinking temporarily and can not serve as a fundamental solution. Also, additional ground pits can occur on ground that is reinforced using this method due to faulty backfill material or faulty hardening. This study used Eco-friendly High-Strength Material (EHSM) as restoration material that can be used in the restoration of underground cavities that have occurred due to ground subsidence to analyze the engineered characteristics of modified dredging clay and test pieces made from changed ratios of EHSM and weathered granite soil were uniaxial compression tests were conducted and freezing-thawing tests were conducted to study strength properties according to environmental changes of restoration material, and after tests were concluded by each level, uniaxial compression tests and dynamic elasticity tests were conducted for intensity analysis. Also, to evaluate strength characteristics of the restored ground, dynamic plate load tests were conducted to verify the improvement effectiveness of the restored ground.

• Archives of Plastic Surgery
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• v.37 no.4
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• pp.391-395
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• 2010

Purpose: Given that the critical nature of the microvascular anastomosis to what is often a long and difficult reconstructive operation, trainees need to have a high level of microsurgical competence before being allowed to perform microsurgery on patients. Some artificial substitutes and dead or live animal models have been used to improve manual dexterity under the operating microscope. Yet, most surgeons are not equipped with such models, so search for easy available and appropriate microsurgical practice model have been an issue. Umbilical artery, placental vessels and gastroepiploic arteries have been previously suggested as a microsurgical training model, which involves other surgical departments. The purpose of this article is to introduce that saphenous vein specimen obtained from varicose vein surgery is useful and has many advantages as training model for the practice of microvascular anastomosis. Methods: The conventional technique using perforation/inversion method with a metallic stripper is widely performed for varicose vein patients. The stripper is inserted through disconnected safeno-femoral junction and retrieved at the knee or the medial side of ankle. The length of saphenous vein specimens removed is about that of one's leg and inversed from inside out. Obtained saphenous vein specimens are re-inversed and cleansed with normal saline, to be readily available for microsurgical practice. Preserved in a squeezed wet saline gauze and refrigerated, frozen or glycerated specimens were investigated into their comparative quality for microsurgical practice. Results: Varicose vein surgery remains one of the common operations performed in the field of plastic surgery. Convenient informed consent regarding the vessel donation can be easily signed. The diameter of the obtained saphenous vein is as variable as 1.5 to 6 mm, which is already stripped, and is in sufficient length corresponding to that of patient's leg. Vessels specimens were available for microsurgical practice within 1 week period when preserved with squeezed wet saline gauze, and the preservation period could be extended monthly by freezing it. Conclusion: Saphenous vein obtained from varicose vein patients provide with variable size of vessel lumen with sufficient length. The practice can be cost effective and does not require microsurgical laboratory. Additionally there is no need of involving other surgical departments in acquiring vessel specimens. Furthermore, simple preservation method of refrigerating for a week or freezing with squeezed wet saline gauze for a month period, allow the saphenous vein obtained after varicose vein surgery as an excellent model for the microsurgical practice.