• Title/Summary/Keyword: Free strain

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A Novel Lactobacillus casei LP1 Producing 1,4-Dihydroxy-2-Naphthoic Acid, a Bifidogenic Growth Stimulator

  • Kang, Jo-Eun;Kim, Tae-Jung;Moon, Gi-Seong
    • Preventive Nutrition and Food Science
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    • v.20 no.1
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    • pp.78-81
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    • 2015
  • 1,4-Dihydroxy-2-naphthoic acid (DHNA) is a bifidogenic growth stimulator (BGS) and could be a functional food ingredient since bifidobacteria are beneficial for human health. For that reason, lactic acid bacteria producing DHNA have been screened. A lactic acid bacterium LP1 strain isolated from a natural cheese was confirmed to produce DHNA, analyzed by a HPLC method. The strain was identified as Lactobacillus casei by 16S rRNA gene sequence analysis. The cell-free supernatant of fermented whey produced by L. casei LP1 presented the BGS activity for three bifidobacterial strains such as Bifidobacterium longum subsp. infantis KCTC 3127, Bifidobacterium bifidum KCTC 3202, and Bifidobacterium breve KCTC 3220 which were human-originated. To the best of our knowledge, a L. casei strain which can produce DHNA was firstly identified in this study.

Stress Measurement of films using surface micromachined test structures (표면 미세 가공된 구조체를 이용한 박막의 응력 측정)

  • 이창승;정회환;노광수;이종현;유형준
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 1996.11a
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    • pp.721-725
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    • 1996
  • The microfabricated test structures were used in order to evaluate the stress characteristics in films. The test structures were fabricated using surface micromachining technique, including HF vapor phase etching as an effective release method. The fabricated structures were micro strain gauge, cantilever-type vernier gauge and bridge for stress measurement, and cantilever for stress gradient measurement. The strain was measures by observing the deformation of the structures occurred after release etching and the amount of deformation can be detected by micro vernier gauge, which has gauge resolution of 0.2${\mu}{\textrm}{m}$. The detection principles and the degree of precision for the measured strain were also discussed. The characteristics of residual stress in LPCVD polysilicon films were studied using these test structures. The stress gradient due to the stress variation through the film thickness was calculated by measuring the deflection at the cantilever free end.

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Carbon Nanoscrolls from CVD Grown Graphene

  • Jang, A-Rang;Shin, Hyeon-Suk;Kang, Dae-Joon
    • Proceedings of the Korean Vacuum Society Conference
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    • 2012.02a
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    • pp.574-574
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    • 2012
  • We report a simple way of fabricating high-quality carbon nanoscrolls (CNSs) by taking advantage of strain relief due to large difference in strain at the interface of graphene and underlying layer. This method allows strain-controlled self rolling-up of monolayer graphene during etching process at predefined positions on SiO2/Si substrates by photolithography. The size and the length of the CNSs can be easily controlled by adjusting the thickness of the underlying layer and by pre-patterning. Raman spectroscopy studies show that the CNSs is free of significant defects, and the electronic structure and phonon dispersion are slightly different from those of two-dimensional graphene. The preparation of high-quality CNSs may open up new opportunities for both fundamental and applied research of CNSs.

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Improved phenomenological modelling of transient thermal strains for concrete at high temperatures

  • Nielsen, Claus V.;Pearce, Chris J.;Bicanic, Nenad
    • Computers and Concrete
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    • v.1 no.2
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    • pp.189-209
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    • 2004
  • Several extensions to the Thelandersson phenomenological model for concrete under transient high temperatures are explored. These include novel expressions for the temperature degradation of the elastic modulus and the temperature dependency of the coefficient of the free thermal strain. Furthermore, a coefficient of thermo mechanical strain is proposed as a bi-linear function of temperature. Good qualitative agreement with various test results taken from the literature is demonstrated. Further extensions include the effects of plastic straining and temperature dependent Poisson's ratio. The models performance is illustrated on several simple benchmark problems under uniaxial and biaxial stress states.

Molecular cloning of phospho-$\beta$-galactosidase gene of lactobacillus casei in escherichia coli (Lactobacillus casei의 phospho-$\beta$-galactosidase 유전자의 대장균내 분자클로닝)

  • 문경희;박정희;최순영;이유미;김태한;김연수;민경희
    • Korean Journal of Microbiology
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    • v.27 no.3
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    • pp.188-193
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    • 1989
  • Gene for lactose catabolism in Lactobacillus casei SW-M1 was encoded by a 60Kb metabolic plasmid. A derivative of only 10kb, pPlac 15 of recombinant plasmid, was constructed by introducing into pBR322 and was cloned into E. coli using restriction endonuclease Pst I. A 10kb insery DNA in plasmid pBR322 was identified as a gene encoded phospho-$\beta$-galactosidase by the determination of enzyme activity. Phospho-$\beta$-galactosidase was apparently expressed in E. coli. The enzyme activities of cell-free extract from transformant E. coli HB101 carrying pPLac 15 DNA were not different from that of L. casei as a donor strain on the basis of enzyme properites. However, specific activity of phospho-$\beta$-galactosidase in the cloned strain with Lac $Y^{-}$ phenotype of E. coli HB101 was lower than that in L. casei strain.

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Vibration analysis of nonlocal strain gradient porous FG composite plates coupled by visco-elastic foundation based on DQM

  • Abdulrazzaq, Mohammed Abdulraoof;Muhammad, Ahmed K.;Kadhim, Zeyad D.;Faleh, Nadhim M.
    • Coupled systems mechanics
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    • v.9 no.3
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    • pp.201-217
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    • 2020
  • This paper employs differential quadrature method (DQM) and nonlocal strain gradient theory (NSGT) for studying free vibrational characteristics of porous functionally graded (FG) nanoplates coupled by visco-elastic foundation. A secant function based refined plate theory is used for mathematical modeling of the nano-size plate. Two scale factors are included in the formulation for describing size influences based on NSGT. The material properties for FG plate are porosity-dependent and defined employing a modified power-law form. Visco-elastic foundation is presented based on three factors including a viscous layer and two elastic layers.The governing equations achieved by Hamilton's principle are solved implementing DQM. The nanoplate vibration is shown to be affected by porosity, temperature rise,scale factors and viscous damping.

On the structural behavior of ship's shell structures due to impact loading

  • Lim, Hyung Kyun;Lee, Joo-Sung
    • International Journal of Naval Architecture and Ocean Engineering
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    • v.10 no.1
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    • pp.103-118
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    • 2018
  • When collision accident between ships or between ship and offshore platform occurs, a common phenomenon that occurs in structures is the plastic deformation accompanied by a large strain such as fracture. In this study, for the rational design against accidental limit state, the plastic material constants of steel plate which is heated by line heating and steel plate formed by cold bending procedure have been defined through the numerical simulation for the high speed tension test. The usefulness of the material constants included in Cowper-Symonds model and Johnson-Cook model and the assumption that strain rate can be neglected when strain rate is less than the intermediate speed are verified through free drop test as well as comparing with numerical results in several references. This paper ends with describing the future study.

A co-rotational 8-node assumed strain element for large displacement elasto-plastic analysis of plates and shells

  • Kim, K.D.
    • Structural Engineering and Mechanics
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    • v.15 no.2
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    • pp.199-223
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    • 2003
  • The formulation of a non-linear shear deformable shell element is presented for the solution of stability problems of stiffened plates and shells. The formulation of the geometrical stiffness presented here is exactly defined on the midsurface and is efficient for analyzing stability problems of thick plates and shells by incorporating bending moment and transverse shear resultant force. As a result of the explicit integration of the tangent stiffness matrix, this formulation is computationally very efficient in incremental nonlinear analysis. The element is free of both membrane and shear locking behaviour by using the assumed strain method such that the element performs very well in the thin shells. By using six degrees of freedom per node, the present element can model stiffened plate and shell structures. The formulation includes large displacement effects and elasto-plastic material behaviour. The material is assumed to be isotropic and elasto-plastic obeying Von Mises's yield condition and its associated flow rules. The results showed good agreement with references and computational efficiency.

Nonlocal strain gradient thermal vibration analysis of double-coupled metal foam plate system with uniform and non-uniform porosities

  • Fenjan, Raad M.;Ahmed, Ridha A.;Alasadi, Abbas A.;Faleh, Nadhim M.
    • Coupled systems mechanics
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    • v.8 no.3
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    • pp.247-257
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    • 2019
  • Fee vibrational characteristics of porous steel double-coupled nanoplate system in thermo-elastic medium is studied via a refined plate model. Different pore dispersions called uniform, symmetric and asymmetric have been defined. Nonlocal strain gradient theory (NSGT) containing two scale parameters has been adopted to stablish size-dependent modeling of the system. Hamilton's principle has been adopted to stablish the governing equations. Obtained results from Galerkin's method are verified with those provided in the literature. The effects of nonlocal parameter, strain gradient, foundation parameters, porosity distributions and porosity coefficient on vibration frequencies of metal foam nanoscale plates have been examined.