• Journal of Navigation and Port Research
• /
• v.31 no.6
• /
• pp.485-490
• /
• 2007

The purpose in having a control rod on a buoy system is to control the motion of it. The system may be composed entirely of a single circular cylinder and a long mooring anchor cable. A control rod has one function to perform in meeting its purpose, and that is to develop a control force in consequence of its orientation and movement relative to the water. The forces and moments generated as a result of the effects of mutual interference. then determine the stability characteristics of the body. In this paper, the study of control-rod-attached buoy's 2-dimensional section was accomplished. model tests and numerical simulations had been carried out with different diameters of control rods. and varying the Reynolds number $Re=5,000{\sim}25,000$ based on the cylinder diameter(D=50mm) to predict the performance of the body and the 2 frame particle tracking method Iud been used to obtain the velocity distribution in the flow field. 50mm circular cylinder Iud been used during the whole experiments and measured results had been compared with each other.

• 제어로봇시스템학회:학술대회논문집
• /
• 2005.06a
• /
• pp.2170-2174
• /
• 2005

Selective Laser Sintering (SLS) is a useful rapid prototyping technique for the manufacture of three dimensional (3D) solid objects directly from a scanning data. A new approach called a Selective Multi-Laser Sintering (SMLS) system has been developed at Korea Institute Machinery & Materials (KIMM) as an industrial type SFFS. This SMLS machine is built with a frame, heaters, nitrogen supply part, laser system. This system uses the dual laser and 3D scanner made in $Solutionix^{TM}$ to improve the precision and speed for large objects. The three-dimensional solid objects are made of polyamide powder. The investigation on each part of SMLS system is performed to determine the proper theirs design and the effect of experimental parameters on making the 3D objects. The temperature of the system has a great influence on sintering the polymer. Because the stability of the powder temperature prevents the deformation of each layer, the controls of the temperature in both the system and the powders are very important during the process. Therefore, we simulated the temperature distribution of build room using the temperature analysis with ANSYS program. Selected radiant heater is used to raise temperature of powder to melting point temperature. The laser parameters such as scan spacing, scan speed, laser power and laser delay time affect the production the 3D objects too. The combination of the slow scan speed and the high laser power shows the good results without the layer curling. The work is under way to evaluate the effect of experimental parameters on process and to produce the various objects. We are going to experiment continuously to improve the size accuracy and surface roughness.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2010.06a
• /
• pp.150-150
• /
• 2010

Carbon nanotubes have drawn attention as one of the most promising emitter materials ever known not only due to their nanometer-scale radius of curvature at tip and extremely high aspect ratios but also due to their strong mechanical strength, excellent thermal conductivity, good chemical stability, etc. Some applications of CNTs as emitters, such as X-ray tubes and microwave amplifiers, require high current emission over a small emitter area. The field emission for high current density often damages CNT emitters by Joule heating, field evaporation, or electrostatic interaction. In order to endure the high current density emission, CNT emitters should be optimally fabricated in terms of material properties and morphological aspects: highly crystalline CNT materials, low gas emission during electron emission in vacuum, optimal emitter distribution density, optimal aspect ratio of emitters, uniform emitter height, strong emitter adhesion onto a substrate, etc. We attempted a novel approach to fabricate CNT emitters to meet some of requirements described above, including highly crystalline CNT materials, low gas emission, and strong emitter adhesion. In this study, CNT emitters were fabricated by filtrating an aqueous suspension of highly crystalline thin multiwalled CNTs (Hanwha Nanotech Inc.) through a metal mesh. The metal mesh served as a support and fixture frame of CNT emitters. When 5 ml of the CNT suspension was engaged in filtration through a 400 mesh, the CNT layers were formed to be as thick as the mesh at the mesh openings. The CNT emitter sample of $1{\times}1\;cm^2$ in size was characteristic of the turn-on electrical field of 2.7 V/${\mu}m$ and the current density of 14.5 mA at 5.8 V/${\mu}m$ without noticeable deterioration of emitters. This study seems to provide a novel fabrication route to simply produce small-size CNT emitters for high current emission with reliability.

• Journal of Plant Biotechnology
• /
• v.7 no.1
• /
• pp.67-74
• /
• 2005

To overcome various disadvantages of conventional cul-ture vessels for micropropagation, a novel disposable vessel, the 'Vitron', made of a multi-layered $OTP^{(R)}$ film and supported by a polypropylene frame, was developed. The film possesses superior properties such as: high light transmittance, low water vapor transmittance and thermal stability and in particular, high gas-permeability. Single nodal explants, which were excised from the multiple shoots derived from shoot-tip culture, were cultured in Vitron and polycarbonate vessels on $3\%$ sugar-containing agar on MS medium and placed at 3000 ppm $CO_2$-enrichment at a low photosynthetic photon flux density (PPFD) ($45{\mu}mol\;m^{-2}\;s^{-1}$). The in vitro and ex vitro growth, and the net photosynthetic rate of in vitro and ex vitro plantlets were significantly enhanced in the Vitron compared to those cultured in a polycarbonate vessel. Explants that were cultured on the same MS medium under low PPFD at various $CO_2$ concentrations were also cultured at 3000 ppm $CO_2$- enrichment at various PPFD: 30, 45, 60, 75 and $90{\mu}mol\;m^{-2}\;s^{-1}$. The best in vitro and ex vitro growth obtained for 3000 ppm $CO_2$-enrichment at $75{\mu}mol\;m^{-2}\;s^{-1}$ PPFD. The novel Vitron vessel, when placed under the two conditions, may replace conventional culture vessels for the successful micropropagation of sweetpotato.

• Informatization Policy
• /
• v.23 no.3
• /
• pp.43-63
• /
• 2016

This research analyzes the international standardization process by observing the international communication sources in order to understand the reason of its current poor use. Using the 'organizing theory' frame, we observe the initial discourse on the need of internationalized domain names in ICANN and the process of negotiation for technical choice of layers. Lastly, we take Korean domain names as a case study in order to understand the conflict and the cooperation of different stakeholders. We summarize the factors of failure of IDN as follows. First, the need of IDN in the beginning was raised around non-English speaking countries, in Asia and Middle East, with the discourse on 'digital divide solution and cultural value' ICANN rather pursues the 'technical stability of Internet Infrastructure', which made its standardization take as long as 10 years. As a result, a variety of standards and services are proposed in the marketplace, which engendered inefficient competition and domain name-related disputes such as cybersquatting, technical instability and confusion of users. In addition, the government agencies fail to present the appropriate policies adjusting multiple interests of different stakeholders.

• 한국생물공학회:학술대회논문집
• /
• 2002.04a
• /
• pp.457-460
• /
• 2002

In an effort to isolate novel strains expressing a thermostable esterase that hydrolyzed the rac-ketoprofen ethyl ester to ketoprofen in the stereospecific manner, we screened various soils and composts from broad ecological niches in which the activity was expected to be found. Three hundreds of microbial strains were tested to determine their ester-hydrolyzing activity by using an agar plate containing insoluble tributyrin as an indicative substrate, and then further screened by activity on the (R,S)-ketoprofen ethyl ester. Twenty-six strains were screened primarily at high growth and incubation temperature and further compared the ability to ethyl ester-hydrolyzing activity in terms of conversion yield and chiral specificity. Consequently, a strain JYl44 was isolated as a novel strain that produced a (R)-stereospecific esterase with high stability and systematically identified as a Bacillus stearothermophilus JY144. The enzyme indeed stables at a broad range of temperature, upto 65 $^{\circ}C$, and pH ranging from 6.0 to 10.0. The optimal temperature and pH for enzymatic conversion were 50 $^{\circ}C$ and 9.0, respectively. Based on the observations that resulted a poor cell growth, and enzyme expression in wild type strain, we further attempted the gene cloning into a general host Escherichia coli and determined its primary structure, concomitantly resulting a high level expression of the enzyme. The cloned gene had an open reading frame (250 amino acids) with a calculated molecular mass of 27.4 kDa, and its primary structure showed a relative high homology (45-52 %) to the esterases from Streptomyces and Bacillus strains. The recombinant whole cell enzyme could efficiently convert the rac-ketoprofen ethyl ester to (R)-ketoprofen, with optical purity of 99 % and yield of 49 %.

• Proceedings of the Korean Institute of Building Construction Conference
• /
• 2008.05a
• /
• pp.35-41
• /
• 2008

Qualitative enhancement of dwelling life has changing the recognition for the environment friendly wood which is being highlighted for its usage as an interior materials. This trend may prove the excellent performance of wood whose inherent characteristics has its comfortable, mild feeling of material, sound resistance and stabilities and the market of interior woods including floor, moulding and wooden panel as finishing interior materials is growing sustainably. However, since this materials is vulnerable to humidity and flame, waterproofing and flame retarding stability, an essential condition for interior materials, together with maintenance, are the main topics to be resolved. From the above-mentioned results, as a result of waterdrop contact angle, wood absorption volume and water content percentage test and the performance test of the processed materials after flame retardant, though there was some submerging time changes among types of woods for ensuring waterproofing performance improvement but as time passes, similar tendency was noticed to be formulated. As the submerging time is increased, so does the absorption volume and accordingly optimal level of range is judged to be drawn in order to ensure excellent performance, taking optimal economy into consideration. Therefore, it is considered that above-mentioned woods could be utilized for waterproof and flame retardant processed interior materials using uniform microwave and in order to put this technology into practical application, a research by way of diversified performance proving is required to be carried out.

• Journal of KIISE:Information Networking
• /
• v.41 no.4
• /
• pp.151-166
• /
• 2014

The present method of measuring QoE (Quality of Experience) was to measure the whole block from the server that provides service to the terminal that the client gets the service. In this way, it was possible to simply determine whether the quality of QoE was good or not, and when the quality of QoE became bad, there was no way of presenting solutions. Also, as QoE metrics are numerous, there has been no strict regulation on how to use. This study analyzed QoE metrics in the viewpoints of network and clients, subdivided the whole service into three phases including one from the server providing the service to the router, another from the router to the terminal getting the service, and the third from the service router to the client router, and presented QoE metric frames appropriate for each phase. Through this, in the KOREN-CERNET environment, this study designed and embodied QoE network and demonstrated stability of QoE network, reduction in client complaint settlement time, and content adjustment effect according to the network change.

• Journal of Korean Society of Steel Construction
• /
• v.16 no.5 s.72
• /
• pp.683-694
• /
• 2004

Advanced analysis and optimal design of semi-rigid space steel frames were presented. The advanced analysis can predict the combined nonlinear effects of connection, geometry, and material on the behavior and strength of semi-rigid frames. The Kishi-Chen power model was used to describe the nonlinear behavior of semi-rigid connections. Geometric nonlinearity was determined using stability functions. Material nonlinearity was determined using the Column Research Council (CRC) tangent modulus and the parabolic function. The direct search method proposed by Choi and Kim was used as optimization technique. One by one, the member with the largest unit value evaluated using the LRFD interaction equation were placed adjacent to a larger member selected from the database. The objective function was assumed to be the weight of steel frame, while the constraint functions were load-carrying capacities, deflections, inter-story drifts, and the ductility requirements. The member sizes determined using the proposed method were compared to those derived from the conventional LRFD method.

• Journal of Microbiology and Biotechnology
• /
• v.20 no.12
• /
• pp.1653-1663
• /
• 2010

The first gene (${\alpha}$-gal1) encoding an extracellular ${\alpha}$-Dgalactosidase from the thermophilic fungus Talaromyces emersonii was cloned and characterized. The ${\alpha}$-gal1 gene consisted of an open reading frame of 1,792 base pairs interrupted by six introns that encoded a mature protein of 452 amino acids, including a 24 amino acid secretory signal sequence. The translated protein had highest identity with other fungal ${\alpha}$-galactosidases belonging to glycosyl hydrolase family 27. The ${\alpha}$-gal1 gene was overexpressed as a secretory protein with an N-terminal histidine tag in the methylotrophic yeast Pichia pastoris. Recombinant ${\alpha}$-Gal1 was secreted into the culture medium as a monomeric glycoprotein with a maximal yield of 10.75 mg/l and purified to homogeneity using Hisbinding nickel-agarose affinity chromatography. The purified enzyme was maximally active at $70^{\circ}C$, pH 4.5, and lost no activity over 10 days at $50^{\circ}C$. ${\alpha}$-Gal1 followed Michaelis-Menten kinetics ($V_{max}\;of\;240.3{\mu}M/min/mg,\;K_m\;of\;0.294 mM$) and was inhibited competitively by galactose ($K_m{^{obs}}$ of 0.57 mM, $K_i$ of 2.77 mM). The recombinant T. emersonii ${\alpha}$-galactosidase displayed broad substrate preference, being active on both oligo- and polymeric substrates, yet had strict specificity for the ${\alpha}$-galactosidic linkage. Owing to its substrate preference and noteworthy stability, ${\alpha}$-Gal1 is of particular interest for possible biotechnological applications involving the processing of plant materials.