• Asian-Australasian Journal of Animal Sciences
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• 제32권8호
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• pp.1095-1103
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• 2019

Objective: Among stress responses, the unfolded protein response (UPR) is a well-known mechanism related to endoplasmic reticulum (ER) stress. ER stress is induced by a variety of external and environmental factors such as starvation, ischemia, hypoxia, oxidative stress, and heat stress. Inositol requiring enzyme $1{\alpha}$ ($IRE1{\alpha}$)-X-box protein 1 (XBP1) is the most conserved pathway involved in the UPR and is the main component that mediates $IRE1{\alpha}$ signalling to downstream ER-associated degradation (ERAD)- or UPR-related genes. XBP1 is a transcription factor synthesised via a novel mechanism called 'frame switch splicing', and this process has not yet been studied in the horse XBP1 gene. Therefore, the aim of this study was to confirm the frame switch splicing of horse XBP1 and characterise its dynamics using Thoroughbred muscle cells exposed to heat stress. Methods: Primary horse muscle cells were used to investigate heat stress-induced frame switch splicing of horse XBP1. Frame switch splicing was confirmed by sequencing analysis. XBP1 amino acid sequences and promoter sequences of various species were aligned to confirm the sequence homology and to find conserved cis-acting elements, respectively. The expression of the potential XBP1 downstream genes were analysed by quantitative real-time polymerase chain reaction. Results: We confirmed that splicing of horse XBP1 mRNA was affected by the duration of thermal stress. Twenty-six nucleotides in the mRNA of XBP1 were deleted after heat stress. The protein sequence and the cis-regulatory elements on the promoter of horse XBP1 are highly conserved among the mammals. Induction of putative downstream genes of horse XBP1 was dependent on the duration of heat stress. We confirmed that both the mechanisms of XBP1 frame switch splicing and various binding elements found in downstream gene promoters are highly evolutionarily conserved. Conclusion: The frame switch splicing of horse XBP1 and its dynamics were highly conserved among species. These results facilitate studies of ER-stress in horse.

• 전산구조공학
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• 제10권4호
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• pp.165-175
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• 1997

본 연구에서는 현재 자동차구조에 사용되고 있는 점용접 보강 리어사이드 프레임, 비보강 리어사이드 프레임 및 레이저 용접을 이용한 이종두께 리어사이드 프레임의 응력, 좌굴 및 진동해석이 수행되었다. 응력 및 진동해석의 경계조건은 양단고정이며, 좌굴해석시에는 단순지지 경계조건을 사용하였다. 구조해석에는 ANSYS 5.0 Code를 사용하였다. 점용접된 리어사이드 프레임의 최대응력은 메인프레임에서 발생하였으며, 그값은 80.9MPa이다. 이때의 최대변형률은 501.mu.이다. 이종두께 레이저 용접된 리어사이드 프레임은 두께가 1.8mm일 때 점용접 보강 리어사이드 프레임의 최대응력과 같아진다. 따라서 동일등가 응력을 기준으로 할 때 레이저 용접을 이용하면 중량을 17.2% 줄일 수 있다. 양단 단순지지된 보강 점용접 리어사이드 프레임의 좌굴하중은 52.54kN이다. 이때 동일한 좌굴하중을 갖는 이종두께 레이저 프레임의 두께는 1.9mm이다. 따라서 중량을 15% 감소시킬 수 있다. 보강된 점용접 리어사이드 프레임의 고유진동수는 163.6Hz로 굽힘모드이며, 이종두께 레이저 용접 리어사이드 프레임의 고유진동수는 179.8Hz이다.

• Steel and Composite Structures
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• 제2권1호
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• pp.35-50
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• 2002

A series of tests on simple-welded plate specimens (SWPS) and T-stub tension specimens simulating some of the joint details in moment frame connections were conducted in this investigation. The effects of weld strength mismatch and weld metal toughness on structural behavior of these specimens were considered under both static and dynamic loading conditions. Finite element analyses were performed by taking into account typical weld residual stress distributions and weld metal strength mismatch conditions to facilitate the interpretation of the test results. The major findings are as follows: (a) Sufficient specimen size requirements are essential in simulating both load transfer and constraint conditions that are relevant to moment frame connections, (b) Weld residual stresses can significantly elevate stress triaxiality in addition to structural constraint effects, both of which can significantly reduce the plastic deformation capacity in moment frame connections, (c) Based on the test results, dynamic loading within a loading rate of 0.02 in/in/sec, as used in this study, premature brittle fractures were not seen, although a significant elevation of the yield strength can be clearly observed. However, brittle fracture features can be clearly identified in T-stub specimens in which severe constraint effects (stress triaxiality) are considered as the primary cause, (d) Based on both the test and FEA results, T-stub specimens provide a reasonable representation of the joint conditions in moment frame connections in simulating both complex load transfer mode and constraint conditions.

• 한국자기학회지
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• 제11권2호
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• pp.50-57
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• 2001

강자성 극박소재에 유기된 잔류응력을 검출하기 위하여 자기유도형 탐촉자(probe)를 설계·제작하였으며, 이를 장착한 잔류음력 평가시스템은 박판내에 유기된 잔류응력의 주응력 방향과 크기를 효과적으로 결정하여 준다. 이 시스템을 반도체 칩 팩키지(package)용으로 사용하는 Fe-42Ni계 lead frame 판재의 잔류응력 검출에 적용한 결과, 양호재 및 잔류응력과다 불량재에 대한 출력전압은 명확히 구별되었고, 잔류응력이 축적된 판재는 양호재와 비교하여 응력의 분포 및 크기에 있어 항상 차이를 나타내었다. 이러한 차이는 탐촉자에 투입하는 전류와 주파수의 함수로 잘 분해되어질 수 있음을 확인하였다.

• 한국디지털건축인테리어학회논문집
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• 제12권4호
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• pp.67-75
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• 2012

The damage of greenhouse has been increasing due to frequent collapse of frame in greenhouse caused by the heavy snow and strong wind. But, greenhouses are constructed by steel tube members of pipe style and pin connection of them, so these greenhouses are very weak. Therefore, this study was carried out to find the type of member section and structural frame system in stress tolerant greenhouses. The modeling types for analysis were designed in accordance with structural frame configuration and member section in greenhouse. These types of models, which are existing type, diagrid type, symmetric and asymmetric section type of frame member in greenhouse were classified. Displacement analysis varying the vertical and horizontal loads for a series of models was carried out. As a result of this paper, it was verified that the structural frame configuration of diagrid type and asymmetric type of member section is better than existing type in the frame of greenhouses against snow loads and wind loads.

• International journal of advanced smart convergence
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• 제12권4호
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• pp.443-450
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• 2023

One of the basic tasks in the automobile manufacturing process is to design a bare chassis, which is the basic frame of a vehicle, and a bracket is a member connecting various devices to the frame. Bracket, which is a member connecting the engine, transmission, and suspension, which are the core devices of driving and operating the vehicle, to the frame, must maintain safety during vehicle operation. If the bracket connecting the various devices constituting the vehicle to the frame does not have durability, serious accidents may occur during operation of the vehicle. In this study, we performed stress analysis on the brackets installed in the bare chassis of the 25-passenger bus in the development stage. Based on the stress analysis performed, an improved bracket dimension was proposed.

• 한국생산제조학회지
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• 제8권1호
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• pp.142-149
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• 1999

A machine frame has been manufactured by casting. However, due to the development of the industrial society, 3-D duties was refused. Especially, the declination of the casting industry makes it difficult to produce the frame. Many companies still manufacture the small casting products. The large casting products are extremely limited and manufactured for their own use. Therefore, it is difficult to keep the term of order. In this study, the characteristics of steel structure which is produced by welding were identified in the view of mechanical strength of steel structure which is produced by welding were identified in the view of mechanical strength to substitute steel structure for casting frame. But welding structure has the residual stress, HAZ and welding deformation. Residual stress and HAZ especially cause crack growth. The proposed steel structure, based on the simulation and experiment(tensile curve and S-N curve), can reduce not only the producting term but also the weight of the frame.

• 한국생산제조학회지
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• 제7권3호
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• pp.104-109
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• 1998

This document is a experimental study on fatigue life of brace mounting weld joint. A brace is used to put sub-frame together on the main frame with high strength bolts. It has low fatigue life so a patch is in need for improving a fatigue characteristics of welded joint in brace mounting This paper presents the most pertinent patch size for truck. For this, a critical stress is computed at the point of fatigue crack occurred on truck frame by finite Element Analysis. Using by this critical stress. Designers are able to determin whether fatigue crack is occurred and are able to select a pertinent patch type. And then, with a selected patch type, structural joint stiffness was estimated to compare to the conventional and other patch type or brace mounting Finally, fatigue test were performed to prove a suitability of selected prototype compare with the conventional and other patch type or brace mounting.

• 한국안전학회지
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• 제26권2호
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• pp.1-5
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• 2011

In order to evaluate fatigue endurance for an ultra-light weight inline skate frame, FEM analysis was performed. Tensile properties and a S-N curve were determined through tensile and fatigue tests on a modified Al-7075+$S_c$ alloy. The yield and ultimate tensile strengths were 553.3 MPa and 705.5 MPa, respectively. The fatigue endurance limit of this alloy was 201.2 MPa. For evaluating the fatigue endurance of the inline skate frame, the S-N data were compared with the stress analysis results through FEM analysis of the frame. The maximum Von-Mises stress of the frame was determined 106 MPa through FEM analysis of the frame, assuming that the rider weight is 75 Kg. Conclusively, on the basis of fatigue limit, the inline skate frame has a safety factor of approximately 2.0.

• 한국산학기술학회논문지
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• 제14권1호
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• pp.57-62
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• 2013

카트 차체에는 현가장치와 차동장치가 존재하지 않으므로 카트주행 중 발생하는 프레임의 변형은 탄성변형으로 인한 주행성능과 프레임의 피로수명에 영향을 미친다. 선회주행 시 비틀림 변형에 의한 카트의 거동은 이 두 가지 변형에 결정적 원인을 제공한다. 이러한 곡선구간에서 카트의 동적 거동을 분석하기 위하여 GPS를 이용하여 차량의 궤적을 추적하고 카트 프레임 강에 작용하는 비틀림 응력을 측정하였다. 레저와 레이싱 카트 프레임의 기계적 성질을 파악하기 위해 재질 분석과 인장시험을 실시하여 각 프레임의 재료 특성을 분석하였다. 비틀림 응력집중과 프레임 비틀림은 프레임 해석을 통해 조사되었다. 또한 주행 분석 장치를 이용하여 레저와 레이싱 카트를 각 조건별로 실차실험을 수행하였고, 이를 통해 곡선구간에서 카트의 주행거동을 분석하였다. 선회주행 시 차량에 원심력에 의한 하중이동의 현상이 발생했으며, 이로 인해 카트 프레임 강에 비틀림 응력이 발생하였다.