• Proceedings of the PSK Conference
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• 2003.10b
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• pp.148.1-148.1
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• 2003

Ceramide is an important lipid messenger involved in mediating a variety of cell functions including apoptosis. Previously, we have shown that ceramide induces Bax translocation which is associated with cytochrome c release from the mitochondria. In this study, we show that p38 MAP kinase is involved in ceramide-induced Bax translocation. In human leukemic cells, ceramide stimulated the phosphorylation of p38 MAP kinase. Preincubation of cells with SB203580, a specific inhibitor of p38 inhibited DNA fragmentation induced by cell-permeable ceramide. (omitted)

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.3
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• pp.25-40
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• 2006

Purpose : This study was undertaken to evaluate the inhibitory effects of Arisaematis rhizoma on the cell proliferation in HeLa cells. Methods : The cultured cell after treatment in the different duration in 24, 48, 72 hours with solution of 1%. 5%, 10% Arisaematis rhizoma was quantified by trypan blue exclusin method. The control group was treated with 2% FBS in the different duration in 24, 48, 72 hours. We examined DNA of activated caspase by FACS analysis, caspase-3 activity, DNA fragmentation by DNA laddering, activity of HeLa Cells by the XTT assay, activity of MAP kinase by RT-PCR analysis. Results : After 72 hours culture, the growth activities of 1%, 5%, 10% Arisaematis rhizoma-treated Hela cell were significantly reduced with control group, respectively. After 24 hours culture, the ratio of cells showing caspase activity by FACS analysis were increased in 1%, 5%, 10% Arisaematis rhizoma-treated Hela cell. It were also increased in 48 hours culture of 10% and 72 hours culture of 5%, 10% Arisaematis rhizoma-treated Hela cell. In 24, 48 and 72 hours culture, DNA fragmentations of 5%, 10% Arisaematis rhizoma-treated Hela cell were obviously observed. These results meaned that Arisaematis rhizoma induces apoptosis of HeLa cells. It was supported by increased caspase-3 activity and decreased MAP kinase activity according to time periods and concentrations of Arisaematis rhizoma solution. Conclusion : The study shows that Arisaematis rhizoma has inhibitory effect on cell proliferation and induction capacity of apoptosis of human cevical carcinoma cell line, HeLa cells, in vitro. These results suggest that Arisaematis rhizoma should be useful for treatment of human cevical carcinoma.

• Journal of KIBIM
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• v.8 no.4
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• pp.72-79
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• 2018

Rapid developments around the world have resulted in urban expansion, habitat destruction, habitat fragmentation, and pollution problems, which are the main reasons for the decline in biological diversity. The United Nations warns that many animals and plants will die out in the near future if this continues. This study was performed to propose a map of eco-corridor suitability analysis of Korean water deer(Hydropotes Inermis) to enhance biodiversity in Chuncheon city. Eight factors affecting habitat suitability were elevation, aspect, slope, forest type, distance to the road, distance to the stream, land use and green connectivity. Previous study analysis on the mobility behaviour of the Korean water deer(Hydropotes Inermis) produced a habitat suitability map by determining the threshold and assigning a value between 0 and 1 depending on the habitat suitability using the fuzzy function. A method of analysis was proposed for a number of eco-corridor through comparative analysis of the data from the produced habitat suitability map and the road-kill point. The previous studies were focused on Backdudaegan region and national parks except for urban cities. The potential habitat map of Korean water deer could be helpful as a way to prevent habitat disconnection and increase species diversity in urban areas.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.1
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• pp.14-30
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• 2006

Purpose : This study was conducted to investigate the inhibitory effects of Cortex ulmi pumilae on cell proliferation in HeLa cell. Methods : Human uterine cervical carcinoma HeLa cells were cultured in the 1%, 5% and 10% concentration of Cortex ulmi pumilae solution for 24 hours, 48 hours and 72 hours for the direct inhibitory effects of Cortex ulmi pumilae. Afterwards, we executed the analysis of the effect of Cortex ulmi pumilae solution on cell proliferation inhibition using XTT assay, DNA fragmentation, molecular biological method through MAP kinase activity and FACS analysis of caspase activity in the HeLa cells. Results : After 48 and 72 hours cultivation, the HeLa cells showed the concentration-dependently significant increase in all Cortex ulmi pumilae solution containing groups compared to the control. In the FACS analysis, all Cortex ulmi pumilae solution containing groups showed concentration-dependent increase compared to the control after 24 hours cultivation and the caspase-3 activities were decreased in all Cortex ulmi pumilae solution containing groups compared to the control after 24, 48 and 72 hours cultivation. After 48 and 72 hours cultivation, we could examined the apparent DNA fragmentation in all Cortex ulmi pumilae solution containing groups. In the XTT study, all Cortex ulmi pumilae solution containing groups showed concentration-dependent decrease compared to the control after 24 and 72 hours cultivation but 10% group after 48 hours and 5% and 10% groups after 72hours were presumed statistically significant differences. The expressions of MAP kinase were decreased in all Cortex ulmi pumilae solution containing groups compared to the control after 24, 48 and 72 hours cultivation. Conclusion : From this study we could suggest that Cortex ulmi pumilae be available to the inhibition of apoptosis of human cervical carcinoma cell line in vitro.

• The Journal of Korean Obstetrics and Gynecology
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• v.28 no.2
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• pp.1-14
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• 2015

Objectives : This study was designed to investigate the effects of Taraxaci Herba (TH) on cell death in breast cancer cells. Methods : In this experiment, the effects of TH on proliferation rates, cell morphology and growth pattern, intracellular reactive oxygen species (ROS) production. In addition, the effects on nuclear condensation, fragmentation and formation of acidic vesicular organelles (AVO) in MCF-7 cells were also investigated. Finally, autophagy related with protein was observed by using western blot method. Results : TH inhibited proliferation of MCF-7 cells, TH elevated intracellular ROS levels significantly. Treatment with TH did not affect nuclear morphologies such as condensation or fragmentation. On the other hand, TH treatment effectively induced AVO. Finally, one of autophagy related with protein, Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A, LC3) level was elevated by treatment with TH. Conclusions : These data indicate that TH is able to be used for patient with breast cancer and mechanisms are involved in autophagy through ROS generation.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.10 no.6
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• pp.120-129
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• 2007

Rapid urbanization has transformed the spatial pattern of urban land use or cover. This paper concentrates that changed characteristics of landscape structure in the Tancheon Watershed, from 1995 to 2003 were investigated using land cover map. We used FRAGSTATS software to calculate landscape indices to characterize the landscape structure. We found that built up area has been increased rapidly during the study period, while cultivated area and forest area have been decreased rapidly in the same period. From 1995 to 2003, built up area was increased from 19.73% to 39.62% and cultivated area and forest area was decreased 17.60% to 5.97% and 58.31% to 49.41%. Number of patches, mean euclidean nearest-neighbor distance, contagion index, Shannon's diversity index increased considerably from 1995 to 2003, also suggesting the landscape in the study area became more fragmented and heterogeneous. but because of continuously fragmentation, landscape became homogeneity. The study demonstrates that landscape metrics can be a useful indicator in landscape monitoring and landscape assessment.

• BMB Reports
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• v.31 no.6
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• pp.560-564
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• 1998

Echinomycin-7 is an echinomycin derivative, Smethylated sulfonium perchlorate of echinomycin. We studied the in vitro cytotoxicity and in vivo antitumor activity of echinomycin-7 against P388 leukemia cells and compared the results with echinomycin. With respect to the cytotoxic effects, echinomycin-7 had cell line-dependent $IC_{50}$ values while echinomycin had similar values to several tumor cell lines. Also, in vivo antitumor activities were observed in tumor-bearing mice treated with both agents, which showed that echinomycin-7 had a broad therapeutic dose range. We also observed the apoptosis on leukemia cells treated with echinomycin-7 which exihibited the ladder pattern of DNA on electrophoresis. In addition to apoptosis, echinomycin-7 arrested $G_1/S$ phases of the cell cycle at the same time. We then examined the signaling pathway of echinomycin-7-induced apoptosis and showed that ERK of the MAP kinase family was activated and translocated into the nucleus by echinomycin-7 stimulation. This study suggests that echinomycin-7 acts as an antitumor agent through in vitro cytotoxicity and has in vivo antitumor activity against leukemia cells, and that the echinomycin-7- induced apoptosis might involve signal transduction via MAP kinases.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.21 no.2
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• pp.39-51
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• 2018

Long-term unexecuted urban parks will be released from urban planning facilities after 2020, this may result in development of those parks. However, little research have been focused on how to develop those parks considering conservation, development, spatial pattern, and so on. Therefore, in this study, we suggested an optimization planning model that minimizes the fragmentation while maximizing the conservation and development profit using ACO (Ant Colony Optimization). Our study area is Suwon Yeongheung Park, which is long-term unexecuted urban parks and have actual plan for private development in 2019. Using our optimization planning model, we obtained four alternatives(A, B, C, D), all of which showed continuous land use patterns and satisfied the objectives related to conservation and development. Each alternative are optimized based on different weight combinations of conservation, development, and fragmentation, and we can also generated other alternatives immediately by adjusting the weights. This is possible because the planning process in our model is very fast and quantitative. Therefore, we expected our optimization planning model can support "spatial decision making" of various issue and sites.

• Applied Biological Chemistry
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• v.51 no.2
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• pp.129-135
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• 2008

Berberine is an isoquinoline alkaloid used in traditional Chinese medicine and has been isolated from a variety of plants, such as Coptis chinensis and Phellodendron amurense. It has a wide spectrum of clinical applications such as in anti-tumor, anti-microbial, and anti-inflammatory activities. However, it is still unknown that berberine related with reactive oxygen species (ROS)-mediated apoptosis pathway in human hepatoma HepG2 cells. In the present study, we are examined the molecular mechanism of ROS- and p38 MAP kinase-mediated apoptosis by berberine in HepG2 cells. Berberine increased cytotoxicity effects by time- and does-dependent manner. $LD_{50}$ was detected 50 ${\mu}M$ at 48h of exposure to berberine. Nuclei cleavage and apoptotic DNA fragmentation were observed in cells treated with 50 ${\mu}M$ of berberine for 48h. Moreover, berberine induced the activating of caspase-3, p53, p38 and Bax expression, whereas the expression of anti-apoptotic signaling pathways, Bcl-2, was decreased. Additionally, berberine-treated cells had an increased level of generation of ROS and nitric oxide (NO). These results indicated that berberine induces apoptosis of HepG2 cells may be mediated oxidative injury acts as an early and upstream change, triggers mitochondrial dysfunction, Bcl-2 and Bax modulation, p38 and p53 activation, caspase-3 activation, and consequent leading to apoptosis.

• Journal of architectural history
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• v.8 no.1 s.18
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• pp.53-62
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• 1999

Modernism in architecture is a very complex and contradictory phenomena. So much so that it has been defined in various ways throughout the history, depending on one's position in the cultural and historical circumstances. It is thus necessary to map out the various concepts of modernism and their relationships in order to have a more comprehensive understanding of modern architecture. This paper attempts to define the various positions as functionalism, formalism and artistic avant-gardism, and to trace their history from the early twentieth century to the present. The change of the concept of modernism from functionalism to artistic avant-gardism seems a logical process in the history of western modem culture. The tendency of contemporary architecture to be more abstract and self referential artistic practice reflects the fragmentation of modern culture and the separation of art and technology. The validity of this position, of course, depends on how one evaluates the role of modern art in the situation of modern culture. It could be viewed either negatively or positively. However, this position is problematic in that it disregards the fundamental differences between architecture and other arts and distanced architecture farther from its material base. Given this historical perspective on the concept of modernism, modernism in Korea should not viewed simply identical to the western modernism, nor should western modernism be imported uncritically. The characteristics of her modernization and their differences from the west should be considered, along with the different status and role of architecture in korean modern society.