• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.37-40
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• 2001

We showed the result that an appreciable difference of secondary structures in the intracrystlline protein was observed between Normal and Deform shells. In order to understand the exact secondary structure of proteins, we investigated the changes in protein conformation from the specific layers via Fourier Self-Deconvolution(FSD) techniques, using ATR(Attenuated Total Reflection) information.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1628-1628
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• 2001

Near infrared (NIR) spectroscopy is a powerful technique for non-destructive analysis that can be obtained in a wide range of environments. Recently, NIR measurements have been utilized as probe for quantitative analysis in agricultural, industrial, and medical sciences. In addition, it is also possible to make practical application on NIR for molecular structural analysis. In this work, Fourier transform near infrared (FT-NIR) measurements were carried out to utilize extensively in the relative amounts of different secondary structures were employed, such as Iysozyme, concanavalin A, silk fibroin and so on. Several broad NIR bands due to the protein absorption were observed between 4000 and $5000\;^{-1}$. In order to obtain more structural information from these featureless bands, second derivative and Fourier-self-deconvolution procedures were performed. Significant band separation was observed near the feature at $4610\;^{-1}$ ,. Particularly the peak intensity at $4525\;^{-1}$ shows a characteristic change with thermal denaturation of fibroin. The structural information can be also obtained by mid-IR and CD spectral. Correlation of NIR spectra with protein structure is discussed.

• Near Infrared Analysis
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• v.2 no.1
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• pp.29-36
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• 2001

This review paper outlines modern pretreatment methods used in NIR spectroscopy. The pretreatment methods can be divided into four categories. One method in is noise reduction. Smoothing is a representative method for the noise reduction. Another is baseline correction. The second derivative and multiplicative scatter correction (MSC) are most frequently employed for baseline correction. The third is centering and normalization and the last is resolution enhancement. Difference spectra, mean centering and second derivative are used in NIR spectroscopy as resolution enhancement methods. In this paper advantages and drawbacks of pretreatment methods currently used in NIR spectroscopy are discussed with many examples of NIR spectra.