• Natural Product Sciences
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• 제28권3호
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• pp.153-160
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• 2022

Neuroinflammation is known to be associated with brain injury in Alzheimer's disease (AD), and the inhibition of microglial activation, a key player in inflammatory response, is considerd as important target for AD. In this study, the ethanol extract of aerial parts of Forsythia velutina Nakai, a Korean native species, significantly inhibited nitric oxide (NO) production in LPS-stimulated BV2 microglial cells. Thus, the active principles in F. velutina aerial parts were isolated based on activity-guided isolation method. As a result, six compounds were isolated and their structures were elucidated based on NMR data and the comparison with the relevant references as arctigenin (1), matairesinol (2), rengyolone (3), ursolic acid (4), secoisolariciresinol (5), and arctiin (6). Among them, four compounds including arctigenin (1), matairesinol (2), secoisolariciresinol (5), and arctiin (6) significantly inhibited NO production in a dose-dependent manner. In particular, matairesinol (2) and secoisolariciresinol (5) reduced 60% of NO production compared to LPS-treated group. This inhibitory effects of matairesinol (2) and secoisolariciresinol (5) were accompanied with the reduced expression levels of iNOS and COX-2. These results suggest that the extract of F. velutina and its active compounds could be beneficial for neuroinflammatory diseases including AD.

• 인간식물환경학회지
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• 제24권4호
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• pp.355-363
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• 2021

Background and objective: This study was conducted on Forsythia velutina, a special plant, in Gyeongsangnam-do Arboretum under the Gyeongsangnam-do Forest Environment Research Institute, which is located in the southern part of Korea. Methods: The research aimed to analyze the flowering characteristics of the plant by calculating the optimal temperature and humidity according to the flowering time and flowering period for 8 years from 2010 to 2017 in order to provide basic data for bioclimate studies of endemic plants. Results: It was observed that the Forsythia velutina showed a life cycle from mid-March and to mid-November. Average growth period was 243 (± 6.5) days. In testing the reliability of a single variable according to the meteorological factors, the Cronbach's Alpha was 0.701, which indicates that the findings were relatively reliable. The average date of flowering was March 16 (SD = 5.8) and the average date on which blossoms fall was March 29 (SD = 5.2). A substantial difference in flowering period was observed from year to year 11 to 23 days, with an average of 16 days (± 4.7). The temperature and humidity in February to March, which affect the flowering, were 2.9-5.5℃, and 66.5-73.0%, respectively, and showed differences every year. Conclusion: The correlation between flowering time and meteorological factors was positive, and the highest daily temperature and average daily temperature had the highest significance. When establishing basic data on plant species for the conservation of endemic plants, the changes in life cycle events and weather conditions are identified. It is believed that it will be helpful in establishing a conservation strategy for the plant species in the future.