• Biomolecules & Therapeutics
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• v.9 no.3
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• pp.194-200
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• 2001

Phellinus linteus (PL)-hot water extract (PL-W) or- methanol extract (PL-M) was orally administered alone (single dose of 400, 800, 1600 mg/kg; 800 mg/kg/day for 5 days) or with cyclophosphamide (CY, 20 mg/kg, i.p.) to female ICR mice. Within PL alone-treated group, WBC and plaque forming cells (PFC) to SRBC were slightly and significantly enhanced when compared with control group. The relative thymus and spleen weights, WBC and PFC numbers were significantly decreased by the treatment of CY, whereas those values were markedly increased by the concomitant treatment of CY and PL when compared with CY administration alone. To assess the effects of PL and/or CY on the mitogen response of splenocytes io LPS, mouse splenocytes were stimulated with or without LPS in the presence of various concentration of PL and/or CY in vitro and splenocytes proliferation (SP) was measured by MTT assay. PL alone increased both SP and LPS- stimulated SP. Moreover, SP and LPS-induced SP suppressed by the treatment of CY alone were significantly restored by PL-treatment. These activities were higher by PL-M than by PL-W, These results indicated that PL was able to increase humoral immunity and to inhibit immunotoxicity induced by CY.

• The Korean Journal of Food And Nutrition
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• v.21 no.3
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• pp.269-274
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• 2008

Plants have long been used as a food source in Korea. In this study, we investigated the combined immunomodulative effects of a water extract mixture of(pine needles, Sedum sarmentosum Bunge, hijkiaorme, buckwheat and Peril a leaves) on Balb/c mice $7{\sim}8$ weeks old. The mice were fed a chow diet ad libitum and the plant extract was orally administered every other day for four weeks at two different concentrations(50 and 500 mg/kg BW). After preparing the single-cell suspension, splenocyte proliferation was determined by the MTT(3-[4,5-di-methylthiazol-2-y]-2,5-diphenyl terazolium bromide) assay. After 48hrs of incubation with the mitogens(ConA or LPS) splenocyte from the mice groups administered 50 and 500 mg/kg BW of the plant extract showed a significant increased in proliferation compared to the control group. A hemolytic plague forming cell assay was used to indicate antibody production against sheep red blood cells(SRBC). The number of antibody-secreting cells T-dependent antigen. The result of this study suggest that supplementation with this plant extract may regulate immune function by increasing splenocyte proliferation and the number of plaque forming cells.

• Journal of Food Hygiene and Safety
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• v.3 no.2
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• pp.83-88
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• 1988

Propyl gallate used as an antioxidant was examined for its effects on murine Immune system and metbemoglobin content treated with anillne. As immunotoxicology assay parameters, we adopted circulating leukocytes and immunoorgan weights for pathtoxicology, IgM plaque forming cells and Artbus reaction for humoral immunity. Propyl gallate's effects were observed as follows; 1. Propyl gallate decreased circulating leukocyte counts, dose dependently. 2. Relative immunoorgan weigbts were not affected. 3. Propyl gallate diminisbed IgM PFCs/spleen cell and IgM PFCs/spleen. 4. Propyl gallate decreased Arthus reaction. 5. Propyl gallate did not affect metbemogiobin content treated wltb aniIIne.

• Transactions of the Korean Society of Automotive Engineers
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• v.16 no.5
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• pp.119-127
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• 2008

In this paper, the influence of the stamping effect is investigated in the performance analysis of a side structure. The analysis covers the performance evaluation such as the crashworthiness and NVH. Stamping analyses are carried out for the center pillar and the side sill made of high strength steels. Then, numerical simulations are carried out in order to identify the stamping effect on the crashworthiness, the natural frequency and the normal mode. The result shows that the analysis of the side structure considering the forming history leads to a different result from that without considering the forming effect. The variation of the system response fully reveals effects of thinning and hardening of members on the performance prediction of vehicle parts. The analysis results demonstrate that the design of auto-body members should be carried out considering the stamping history for accurate assessment of various performances.

• YAKHAK HOEJI
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• v.36 no.2
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• pp.93-109
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• 1992

The purpose of this experiment was to investigate both the immunomodulatory effect of evening primrose(EP) oil and the effects of EP oil on immunoregulation by cyclophosphamide in mice. EP oil at doses of 0.1, 0.2 and 0.4 ml/kg were orally administered to ICR male mice once daily for 28 consecutive days. Cyclophosphamide was injected intraperitoneally to ICR mice with a single dose of 5 mg/kg at 2 days before secondary immunization. Mice were sensitized and challenged with sheep red blood cells(S-RBC). Immnune responses were evaluated by humoral and cellular immune responses and non-specific immune response. The results of this study were summarized as follows; (1) The humoral immune responses such as hemagglutination titer(HA), hemolysin titer(HY), Arthus reaction and plaque forming cell(PFC) were significantly enhanced in the low dose EP oil administered groups(0.1 and 0.2 ml/kg). However, in the high dose EP oil administered group(0.4 ml/kg) the responses were significantly lowered. (2) In the case of cellular immune responses, delayed type hypersensitivity reaction(DTH) was significantly decreased in EP oil whereas rosette forming cell(RFC) was remarkably enhanced. (3) Activities of natural killer cells and phagocyte were generally enhanced in EP oil. In addition, serum albumin and globulin were also increased.

• YAKHAK HOEJI
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• v.57 no.6
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• pp.406-411
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• 2013

Rumex crispus (Curled Dock, Polygonaceae) is a perennial wild plant used in traditional medicine as a laxative, astringent, and to treat blood and skin disease. The ethanol extract of R. crispus was obtained and its carbohydrate contents were analyzed using high-performance anion-exchange chromatography with pulsed amperometric detection. The anabolic effects of R. crispus in human osteoblastic MG-63 cells were investigated using the WST-8 assay, alkalinephosphatase (ALP) assay, and mineralization assay. The ethanol extract increased the proliferation of MG-63 cells and stimulated ALP activity in a dose-dependent manner over a 72-hrs period. Additionally, the ethanol extract dose-dependently stimulated the formation of bone nodules in MG-63 cells treated for 12 days. The ethyl acetate fraction from the ethanol extract did not affect osteoblast viability but induced an increase in ALP activity. In conclusion, the ethanol extract of R. crispus increases the proliferation and bone-forming activity of osteoblasts, and hence it could be used in the development of bone-forming stimulatory nutraceuticals and osteoporosis-related medicines.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2005.05a
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• pp.415-418
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• 2005

In recent years, Magnesium (Mg) and its alloys have become a center of special interest in the automotive industry. Due to their high specific mechanical properties, they offer a significant weight saving potential in modem vehicle constructions. Most Mg alloys show very good machinability and processability, and even the most complicated die casting parts can be easily produced. The die casting process is a fast production method capable of a high degree of automation for which certain Mg alloys are ideally suited. Although Mg alloys are fulfilling the demands for low specific weight materials with excellent machining and casting abilities, they are still not used in die casting process to the same extent as the competing material aluminium. One of the reasons is that effects of various forming variables for die casting process is not closely examined from the viewpoint of die design. In this study, step die and flowability tests for AM60 were performed by die casting process according to various combination of casting pressure and plunger velocity. Microstructure and Victors hardness tests were examined and performed for each specimen to verify effects of forming conditions.

• Molecules and Cells
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• v.28 no.5
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• pp.473-477
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• 2009

Previously, the 9-mer analog peptides, 9Pbw2 and 9Pbw4, were designed based on a defensin-like peptide, protaetiamycine isolated from Protaetia brevitarsis. In this study, antifungal effects of the analog peptides were investigated. The antifungal susceptibility testing exhibited that 9Pbw4 contained more potent antifungal activities than 9Pbw2. A PI influx assay confirmed the effects of the analog peptides and demonstrated that the peptides exerted their activity by a membrane-active mechanism, in an energy-independent manner. As the noteworthy potency of 9Pbw4, the mechanism(s) of 9Pbw4 were further investigated. The membrane studies, using rhodamine-labeled giant unilamellar vesicle (GUV) and fluorescein isothiocyanate (FITC)-dextran loaded liposome, suggested that the membrane-active mechanism of 9Pbw4 could have originated from the pore-forming action and the radii of pores was presumed to be anywhere from 1.8 nm to 3.3 nm. These results were confirmed by 3D-flow cytometric contour-plot analysis. The present study suggests a potential of 9Pbw4 as a novel antifungal peptide.

• The Bulletin of The Korean Astronomical Society
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• v.43 no.2
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• pp.35.2-35.2
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• 2018

Using the K-band Multi-object Spectrograph (KMOS) at the Very Large Telescope (VLT), the KMOS Redshift One Spectroscopic Survey (KROSS) has gathered integral-field data for ~800 star-forming galaxies at a redshift z~1, when the universe was roughly half its current age and forming the bulk of its stars. With spatially-resolved observations, KROSS reveals galaxies that are both gas-rich and highly turbulent. It is possible to derive the observed and baryonic Tully-Fisher (luminosity - rotation velocity) relations, thus constraining the mass-to-light ratios and total (luminous + dark) masses of the galaxies. This in turn highlights the dependence of the relation zero-point on the degree of rotational support of the galaxies (rotational velocity to velocity dispersion ratio). By degrading and analogously analysing integral-field data of hundreds of local galaxies from the Sydney-AAO Multi-object Integral-field Spectrograph (SAMI) survey, a robust comparison z=0 Tully-Fisher relation can also be derived, thus further constraining the luminous and dark mass growth of disk galaxies over the last 7 billions years. This unique comparison also reveals that systematic effects associated with sample selection and analysis methods are as large as the effects expected from cosmological evolution, and thus that most other comparisons employing heterogeneous data and/or methods can safely be ignored.

• Advances in nano research
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• v.14 no.2
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• pp.155-164
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• 2023

Grain size in sheet metals in one of the main parameters in determining formability. Grain size control in industry requires delicate process control and equipment. In the present study, effects of grain size on the formability of steel sheets is investigated. Experimental investigation of effect of grain size is a cumbersome method which due to existence of many other effective parameters are not conclusive in some cases. On the other hand, since the average grain size of a crystalline material is a statistical parameter, using traditional methods are not sufficient for find the optimum grain size to maximize formability. Therefore, design of experiment (DoE) and artificial intelligence (AI) methods are coupled together in this study to find the optimum conditions for formability in terms of grain size and to predict forming limits of sheet metals under bi-stretch loading conditions. In this regard, a set of experiment is conducted to provide initial data for training and testing DoE and AI. Afterwards, the using response surface method (RSM) optimum grain size is calculated. Moreover, trained neural network is used to predict formability in the calculated optimum condition and the results compared to the experimental results. The findings of the present study show that DoE and AI could be a great aid in the design, determination and prediction of optimum grain size for maximizing sheet formability.