Somatic embryogenesis is as an excellent technology for potential use in plant mass production, germplasm conservation, or genetic engineering. We examined the effect of cold storage using 3 embryogenic callus lines with different levels of embryogenesis competence derived from immature zygotic embryo cultures of Kalopanax setemlobus. Somatic embryo induction, germination and plant conversion were evaluated after 1, 3 and 6 months storage at $4^{\circ}C$ in the dark. Most cold-stored embryogenic calli formed somatic embryos normally even after 6 months; however, the induction rate was gradually decreased by increasing the storage period. The most competent line tended to show a slight decline in somatic embryo induction rate, as compared with other lines after cold storage. In general, cold storage resulted in reduced somatic embryo germination and plant regeneration, although 93% somatic embryo germination and 91% plant conversion were achieved regardless of the storage period. Cold storage led to cell browning and degradation. Additionally, the cell structures were confirmed by the aceto-carmine and evans blue dye evaluation. Collectively, our results showed that embryogenic callus of K. septemlobus could be preserved at $4^{\circ}C$ without subculture for 6 months, and suggested the need for storage of relatively more competent embryogenic calli lines to support somatic embryo induction.
Kim, Daesun;Kim, Eun-Sook;Lim, Jong-Hwan;Lee, Yangwon
Korean Journal of Remote Sensing
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v.36
no.5_1
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pp.835-846
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2020
Recent severe climate changes and extreme weather events have caused the uncommon types of forest ecosystem disturbances such as hails and gypsy moths. This paper describes the analysis of the forest ecosystem disturbances using ISODATA (Iterative Self-organizing Data Analysis Technique Algorithm) with the RapidEye and Sentinel-2 images, regarding the cases of the hail damages in Hwasun in 2017 and the gypsy moth damages in the Chiak Mountain in 2020. In the case of hail damages, the comparison of the June image of this study and the July field survey of the previous study showed that the damage severity increased from June to July as the drought overlapped after the trees were injured by the hails. In the case of gypsy moths, significant leaf damages were found from the image of June, and the damages were mainly distributed at the low-altitude slope near Wonju City. We made sure that satellite remote sensing is a very effective method to detect various and unusual forest ecosystem disturbances caused by climate change. Also, it is expected that the Korean Medium Satellite for Agriculture and Forestry scheduled to launch in 2024 can be actively utilized to monitor such forest ecosystem disturbances.
Rose and chrysanthemum are the most popular flowers in Korean floriculture. Sorting flowers is a labor intensive operation in cultivation of the cut flowers and needed to be mechanized. Machine vision is one of the promising solutions for this purpose. This study was carried out to develop hardware and software of a cut flower sorting system using machine vision and to test its performance. Results of this study were summarized as following; 1. Length of the cut flower measured by the machine vision system showed a good correlation with actual length of the flower at a level of the coefficients of determination (R$^2$) of 0.9948 and 0.9993 for rose and chrysanthemum respectively and average measurement errors of the system were about 2% and 1% of the shortest length of the sample flowers. The experimental result showed that the machine vision system could be used successfully to measure length of the cut flowers. 2. Stem diameter of the cut flowers measured by the machine vision system showed a correlation with actual diameter at the coefficients of determination (R$^2$) of 0.8429 and 0.9380 for rose and chrysanthemum respectively and average measurement errors of the system were about 15% and 7.5% of the shortest diameter of the sample flowers which could be a serious source of error in grading operation. It was recommended that the error rate should be considered to set up grading conditions of each class of the cut flowers. 3. Bud maturity of 20 flowers each judged using the machine vision system showed a coincidence with the judgement by inspectors at ranges of 80%∼85% and 85%∼90% for rose and chrysanthemum respectively. Performance of the machine vision system to judge bud maturity could be improved through setting up more precise criteria to judge the maturity with more samples of the flowers. 4. Quality of flower judged by stem curvature using the machine vision system showed a coincidence with the judgement by inspectors at 90% for good and 85% for bad flowers of both rose and chrysanthemum. The levels of coincidence was considered as that the machine vision system used was an acceptable system to judge the quality of flower by stem curvature.
Journal of the Korean Society of Environmental Restoration Technology
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v.19
no.6
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pp.77-86
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2016
Due to aging of the rural and mountain populations the labor force is reduced. However work intensity continues to increase, thus, there is a need to improve the current effectiveness of forest operations. This study compared and analyzed the Operation productivity and efficiency of planting containerized seedlings using a battery-powered planting auger and a mattock. Growth was also investigated by looking into the initial growth increments in the planted seedlings. Tasks were investigated by analyzing the process and operation time needed to plant 1 containerized seedling using a planting auger and a mattock. The time spent on the various elements of the planting process was measured with a stopwatch but observations were done continuously. Result of the study shows that with the use of a lightweight planting auger the average time spent to plant a containerized seedling is 18.61 seconds while with the use of a mattock it took an average of 26.96 seconds which clearly demonstrates that the planting auger is more efficient in terms of working hours. Working intensity was also analyzed with the use of a portable heart rate monitor (Polar V800). The average increase in heart rate and work intensity index were analyzed for one planting cycle. It was observed that when using the lightweight planting auger, there was a 46.51% increase in the average heart rate while a 74.67% increase in heart rate when the mattock was used which shows that there is a significant increase in heart rate when mattock is used. In addition, work intensity index was observed to be 29.95% and 47.83% when the planting auger and mattock were used respectively. With the continuous use, work intensity index is significantly higher with the use of the mattock as compared to that of the lightweight planting auger. There were no significant differences on the growth increment of seedlings planted using the different tools until a year after planting, however differences in growth increment were observed after a year. A difference of 15.1 cm in height and 3.41 mm in diameter was observed which shows that the use of lightweight planting auger is excellent for planting containerized seedlings.
Proceedings of the Korean Society for Agricultural Machinery Conference
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1993.10a
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pp.109-118
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1993
Development process of agricultural technology has been studied with a case study of Korean agriculture. Technological is considered as a transformer of inputs into outputs and hence technological appropriateness, an important aspect of agricultural development strategies, is considered as a dynamic concepts. Considering the concept of agricultural system as a delivery system for providing essential materials and services to producers and consumers, it has been divided into two major groups of dimensions vis. external challenge dimensions and internal response dimensions. Market, investment and agro-ecosystem constitute the external challenge dimensions : whereas trade , technology as well as production and resources allocation constitute internal response dimensions. The system manager is responsible for maintaining equilibrium in the mentioned six sub-systems. Two kinds of alternatives paths of technological development viz. land saving technology and labour saving technolog have been studied. Technology is considered as a combination of four basic components viz. facilities, abilities, facts and frameworks. Adoption of innovation in agriculture depends on profitability, awareness, risk aversion, financial capacity, institutional infrastructure, availability of physical inputs and adaptability to the local conditions. For a cast study of Korea, changes in the agricultural system through external challenge dimensions are investigated. The impacts of industrialization on agro-ecosystem reported are shift of labour from the agricultural sector to non-agricultural sectors and continuously increasing demand of farm the agricultural sector to non-agricultural sectors accompanied by increase in land prices. The impacts on the commodity market discussed are shift in demand from rice, barley and other cereals to meat , dairy products and vegetables : and increasing in supply capacity of agricultural inputs. The process of agricultural development from 1962 to 19 1 9 (i.e. from start of the first to the end of the sixth five year plan) are also discussed in details with several policy measures taken. The trend of agricultural income and productivity are also analyzed. The main cause of increase in the agricultural income is considered as increase in labour productivity. The study revealed that during the span of 1965-88, holding size has not changed significantly, but both the land and labour productivity increased and so did the agricultural income. R&D activities in Korea have changed over time in three stages vix. import of improved technology, localization by adaptive research and technological mastery. For the new technology to be made affordable to farmers, policy measures like fertilizer and food grain exchange system, dual price system in rice and barely and loan for machinery were strengthened.
Jo, Gi-Woong;Kim, Young-Sun;Ham, Soun-Kyu;Bae, Eun-Ji;Lee, Jae-Pil;Kim, Doo-Hwan;Kim, Woo-Sung;Lee, Geung-Joo
Weed & Turfgrass Science
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v.6
no.4
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pp.322-332
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2017
Microbial fertilizer has been used to prompt turfgrass growth and quality and to prevent turfgrass diseases in turfgrass management of golf courses. This study was conducted to evaluate effects of microbial fertilizer containing Lactobacillus fermentum (MFcL) on changes of turfgrass quality and growth by investigating turf color index, chlorophyll index, clipping yield, and nutrient content in the turfgrass tissue. Treatments were designed as follows; non-fertilizer (NF), control fertilizer (CF), MFcL treatments [CF+$1.0g\;m^{-2}$(MFL), CF+$2.0g\;m^{-2}$ (2MFL)], and only MFcL treatment (OMF; $1.0g\;m^{-2}$ MFL). Chemical properties of soil by application of MFcL was unaffected. Turf color index, chlorophyll index, clipping yield and nutrient content and uptake of MFcL treatments were similar to CF. Furthermore, turfgrass shoot density of MFL was increased by 20% than that of CF, and that of OMF by 22% than NF. These results show that the application of microbial fertilizer containing L. fermentum increased turfgrass shoot density, which is not attributed to nutrient uptake in this study, but needs to be further investigated with other mechanisms such as biostimulant induction or phytohormone production.
KIPS Transactions on Software and Data Engineering
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v.6
no.12
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pp.549-564
/
2017
Not only services that are provided by a single system have been various with the development of the Internet of Things and autonomous software but also new services that are not possible before are provided through collaboration between systems. The collaboration between autonomous systems is similar to the ecosystem configuration in terms of biological viewpoints. Thus, it is called the IT Ecosystem, and this concept has arisen newly in recent years. The IT Ecosystem refers to a concept that achieves a mission of each of a number of heterogeneous systems rather than a single system utilizing their own autonomy as well as achieving the objectives of the overall system simultaneously in order to meet a single common goal. In our previous study, we proposed architecture of elementary level and as well as basic several meta-models to implement the IT Ecosystem. This paper proposes comprehensive reference architecture framework to implement the IT Ecosystem by cleansing the previous study. Among them, a utility function based on cost-benefit model is proposed to solve the dynamic re-configuration problem of system components. Furthermore, a measure of using genetic algorithm is proposed as a solution to reduce the dynamic re-configuration overhead that is increased exponentially according to the expansion of the number of entities of components in the IT Ecosystem. Finally, the utilization of the proposed orchestration framework is verified quantitatively through probable case studies on IT Ecosystem for unmanned forestry management.
Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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2006.06b
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pp.231-237
/
2006
Grease barrier food containers are commonly used for packaging of fast food, cooked food, and food in general. Greaseproofing is also used for certificate paper and label paper etc. Different pulp raw materials, due to their different fiber morphology and chemical compositions, produce papers of varying characteristics. We used optical photomicroscopy and fiber analysis data to evaluate fiber morphology and traits under various beating conditions in order to understand which pulp raw materials produced superior greaseproofing property when a fluorinated greaseproofing agent was added internally. The experiment studied 9 species of pulps, including 2 softwood (northern pine and radiata pine) bleached kraft pulps which were beaten to 550 and 350 mL CSF, respectively; 3 hardwoods (eucalypts, acacia, mixed Indonesian hardwoods) bleached kraft pulps which were beaten to 450 and 250 mL CSF, respectively; and nonwood fibers of reed, bagasse, and abaca. A fluorinated greaseproofing chemical at 0.12% dosage with respect to dry pulp was added to each pulp preparation and formed handsheets. A total of 67 sets of handsheets were prepared, and their basis weights, thickness, bulks, opacities, wet opacities, air resistance, water absorption and degrees of greaseproofing were measured for an overall evaluation of pulp and freeness on greaseproofing papers. The experimental fiber length, coarseness and distribution characteristics and the greaseproofing results suggest that softwood pulps (radiate pine > northern pine) were superior to hardwood pulps (eucalypts > acacia > mixed Indonesian hardwoods). The unbeaten pulps gave papers with high porosities and nearly devoid of greaseproofing property. Greaseproofing is proportional to air resistance. Among the nonwood fibers, bagasse had the best greaseproofing property, followed by reed and abaca was the poorest. With regards to waterproofing property, hardwood pulps (mixed Indonesian hardwoods > acacia > eucalypts) were better than softwood pulps (northern pine > radiate pine). Among the Nonwood fibers, reed had the highest waterproofing property, and it was followed by abaca, while bagasse had the poorest waterproofing characteristic. In summary, bleached kraft northern pine, eucalypts and reed pulps were best suited for making greaseproofing papers, Freeness of the pulps should be kept at $200{\sim}280mL$ CSF for optimal performance.
Research was conducted at the Wood Materials and Engineering Laboratory, Washington State University, Pullman, WA to evaluate the effects of the sequence of wax addition, wax level, and wax type on mechanical properties and water resistance performance of isocyanate-bonded particleboard. Mechanical properties and water resistance performance were not influenced significantly by the sequence of wax addition. Internal bond and wet modulus of rupture in bending strength were decreased significantly by increasing the wax emulsion level, but dry modulus of rupture and modulus of elasticity in bending strength were not decreased significantly by increasing the wax emulsion level. Dry internal bond, dry and wet moduli of rupture, and modulus of elasticity were not decreased by increasing the solid wax level except for wet internal bond. The addition of 1.0 and 1.5% wax level did not produce any significant additional water resistance effect when compared to the addition of 0.5% wax level. Internal bond values of boards with solid wax addition showed significantly better results than boards with just a wax emulsion added. Modulus of rupture, modulus of elasticity, and water resistance performance did not show significant difference between solid wax and wax emulsion.
Carbon isotope ratio ($^{13}C/^{12}C$, expressed as ${\delta}^{13}C$) of tree ring can be proxy of atmospheric $CO_2$ concentration ([$CO_2$]) due to the inter-correlation between atmospheric [$CO_2$], ${\delta}^{13}C$ of atmospheric $CO_2$, and ${\delta}^{13}C$ of plant tissue that assimilates atmospheric $CO_2$. This study was conducted to investigate if ${\delta}^{13}C$ of tree ring of Pinus densiflora in polluted area may show a lower value than that in unpolluted area and to explore the possibility of reconstructing atmospheric [$CO_2$] using its relationship with ${\delta}^{13}C$ of tree ring. During the period between 1999 and 2005, ${\delta}^{13}C$ of tree annual ring tended to decrease over time, and the ${\delta}^{13}C$ in polluted area (-27.2‰ in 2009 to -28.3‰ in 2005) was significantly (P<0.001) lower than that (-26.0‰ in 1999 to -27.1‰ in 2005) in unpolluted area. This reflects a greater emission of $CO_2$ depleted in $^{13}C$ in the polluted area. Atmospheric [$CO_2$] was significantly (P<0.01) correlated with ${\delta}^{13}C$ of tree ring in a linear fashion. Using the linear regression equation, atmospheric [$CO_2$] in the polluted area was estimated to range from 392.3 ppm in 1999 to 410.9 ppm in 2005, and these values were consistently higher than the national atmospheric [$CO_2$] monitored at the Anmyoundo meteorological station (from 370.7 ppm in 1999 to 387.2 ppm in 2005). Our study suggested that it is possible to reconstruct atmospheric [$CO_2$] in a certain area using the relationship between tree ring ${\delta}^{13}C$ and atmospheric [$CO_2$].
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