• Journal of Korean Society of Forest Science
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• v.96 no.5
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• pp.585-590
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• 2007

Rhizina undulata is the fungus, which causes Rhizina root rot on coniferous trees. Nested-PCR using ITS-specific primer was applied to detect R. undulata from the soils of Japanese black pine (Pinus thunbergil) forests infested with the disease in Seocheon, Chungnam Province, South Korea. Soil samples were collected from four different sites, both dead trees and fruit bodies of R. undulata were present, dead trees only present, fruit bodies only present, and both were absent. Nested-PCR products specific to R. undulata ITS-region were amplified. Positive reactions were found in some samples from the sites, where dead trees and fruit bodies of R. undulata were absent as well as where both of those were present. R. undulata was mainly detected in the soil samples from the depth of 5~20 cm under the soil surface. These results show that the nested-PCR could be used to diagnose the presence or potential infestation of R. undulata in the soils of pine forests.

• Korean journal of applied entomology
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• v.47 no.2
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• pp.127-131
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• 2008

Ambrosia beetle, Platypus koryoensis, is a vector of oak wilt disease caused by Raffaelea sp. in Korea. The degree of damage by oak wilt disease was dependent on the density of the beetles in the oak trunk, a relationship between the degree of damage by oak wilt disease and the density of beetle on the basis of the number of entry hole was studied. Entry hole distribution within tree trunk was analyzed by the nearest neighbor method. Thirty four oak trees (Quercus mongolica) located in survey area were selected and then degree of damage, the number of attack hole/$623cm^2$ in upper (50cm from the surface) and lower (surface) trunk and the nearest neighbor distance between the holes were measured. The number of hole and the nearest neighbor distance in upper and lower part were positively correlated with each other. As the degree of damage was severer, the number of the holes increased, whereas the nearest neighbor distance decreased. The distribution pattern of the hole was changed from clumped one to uniform as the severity of damage increased. These results suggested that Platypus koryoensis attacked the oak tree in concentrative manner at initial stage of attack but at final stage, it distributed uniformly to reduce intraspecific competition between the beetles.

• Research in Plant Disease
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• v.20 no.4
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• pp.295-298
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• 2014

Oak wilt caused by Raffaelea quercus-mongolicae was first noticed in South Korea in 2004 and, ever since, its distribution and damage have been increasing. To screen a fungicide effective for oak wilt control by tree injection, laboratory and field experiments were conducted. Ten fungicides and one antibiotic were examined in vivo for their effectiveness in restricting the growth of R. quercus-mongolicae and R. quercivora (Japanese oak wilt pathogen) isolates. To the Korean isolates of R. quercus-mongolicae, chlorothalonil showed the highest fungicidal effects, followed by benomyl and propiconazole. To the Japanese one, propiconazole was highest in the fungicidal effectiveness, followed by benomyl and bitertanol. Propiconazole was selected for field-testing of its control efficacy because it showed good fungicidal effects in vitro and systemic activity. The control efficacy in the field was 87.5% in the first year of injection and 66.7% in the second year, indicating the fungicidal effects last at least over one year.

• The Korean Journal of Mycology
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• v.33 no.2
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• pp.98-102
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• 2005

In 2003 to 2005, the root rot of Japanese angelica (Aralia elata) was surveyed in nursery beds of Korea, where incidence of the disease often reached up to 100%. Three isolates were obtained from the infected roots, and identified as Phytophthora cactorum on the basis of cultural, morphological characteristics and molecular analysis. The isolates were characterized by having markedly papillate and broadly ovoid deciduous sporangia. The optimum temperature for mycelium growth was at $25^{\circ}C$ on V8 juice agar. Pathogenicity of the isolates was confirmed by soil mixture inoculation. Approximately 900 bp of ITS rDNA was amplified from all 3 isolates and band pattern of restriction fragments observed by Alu I, Msp I, and Taq I digestion also supported the result of the morphological identification when compared with PhytID database.

• The Korean Journal of Mycology
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• v.48 no.1
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• pp.69-74
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• 2020

In September 2017, a heavy damage by premature defoliation with the zonate leaf spots was observed in several shrubs of Lespedeza cyrtobotrya growing at Mt. Obongsan in Chuncheon, Korea. Numerous cone-shaped, white sporophores of a fungus were observed on lesions of the abaxial leaf surface. A similar fungus was isolated in September 2019 from the leaves of L. cyrtobotrya growing at Mt. Taegisan in Hoengseong, Korea. The morphological characteristics of the sporophores were consistent with those of Grovesinia moricola. The species identification was confirmed by sequencing the internal transcribed spacer (ITS) region of the ribosomal DNA from the two isolates (KACC48417 and KACC48934). The fungal pathogenicity was determined by an artificial inoculation in conditions of relative humidity and temperature of 100% and 15±2℃, respectively. This is the first report of association of G. moricola with L. cyrtobotrya in Korea.

• The Korean Journal of Mycology
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• v.46 no.1
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• pp.69-74
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• 2018

The Rhododendron powdery mildew fungus Erysiphe izuensis has been recorded throughout Asia, including China, Japan, Korea, and Russia. In Korea, E. izuensis has been identified based on morphological characteristics of the anamorph found on introduced Rhododendron spp. We here describe the first identification of E. izuensis collected from Rhododendron yedoense f. poukhanense in Korea. Morphological characteristics of the teleomorph as well as internal transcribed spacer sequences of the species are provided. Moreover, we describe the characteristics of the primary conidia and conidial surface pattern of E. izuensis for the first time.

• Research in Plant Disease
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• v.20 no.4
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• pp.270-274
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• 2014

Division of Forest Diseases & Insect Pests, Korea Forest Research Institute, Seoul 130-712, Korea The objective of this study was to evaluate the virulence of geographically different isolates of oak wilt pathogen, Raffaelea quercus-mongolicae and other Raffaelea species. In this study, mature trees of Quercus mongolica were inoculated with the various isolates of Raffaelea spp. and their virulence was evaluated by measuring the extent of sapwood discoloration resulting from the inoculation. The average length of discolored sapwood in a lateral direction was longest in the trees inoculated with the isolates from Korea (8.69 cm) followed by R. quercivora (7.51 cm) and the other Raffaelea spp. (3.35 cm). The lateral length of discolored sapwood caused by the inoculation with Korean strains varied from 4.71 to 14.90 cm indicating their differences in virulence. The area of discolored sapwood caused by the inoculation with Raffaelea spp. varied from 1.57 to $8.42cm^2$ indicating their differences in virulence. Based on the length and area of the discolored sapwoods, isolated YY and wj43 appeared to have the highest virulence among all the Raffaelea isolates tested. Each of the two isolates was obtained from Gangwon Province and Jeonbuk Province, respectively.

• Research in Plant Disease
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• v.17 no.3
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• pp.410-412
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• 2011

Since the late 2000s, a serious Entomosporium leaf spot disease, caused by Entomosporium mespili, has been found frequently on leaves of Eriobotrya japonica and Photinia glabra at a nursery station in Goheung and Jeju, respectively. Studies were conducted to select fungicides that would effectively control Entomosporium leaf spot. Among the three fungicides tested, weekly foliar applications of propiconazole and chlorothalonil effectively reduced disease severity on E. japonica and P. glabra showing control value of 64.1% and 87.6%, respectively. Weekly treatments of thiopanate methyl were less effective. Propiconazole controlled the disease, but, it was phytotoxic to P. glabra.

• Korean journal of applied entomology
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• v.56 no.2
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• pp.121-133
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• 2017

This study was conducted to investigate the community structure of arthropods, including various insect pests and their natural enemies, in walnut orchards. Furthermore, we tried to compare the species richness and community structure of arthropods by three different sampling methods, including beating, funnel trap, and pitfall trap. Arthropods were surveyed in three walnut orchards located at Gimcheon, Buyeo, and Hwaseong in South Korea from May to September, 2016. A total of 408 arthropod species were identified from 4,372 individuals, and 63 species were collected in all study sites. The species richness was the highest in funnel trap, while the abundance was the highest in pitfall trap. The species composition of the insects collected by the beating method was more similar to that of the funnel trap than the pitfall trap. Although the distributions of the economically important pests were different according to the study sites, six species, including Dichocrocis punctiferalis, Pseudaulacaspis pentagona, Gastrolina depressa, Lycorma delicatula, Metcalfa pruinosa, and Pochazia shantungensis, are the potentially important pests in walnut orchards. We also found a variety of predators and parasitoids, which will be important for walnut pest management.

• The Korean Journal of Soil Zoology
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• v.9 no.1_2
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• pp.1-5
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• 2004

Insect pests and their natural enemies of Hibiscus Linne (Malvaceae) were investigated from March 2002 to November 2004. Fourteen insect pest species of 9 families in 5 orders were collected from Hibiscus syriacus: 5 species in Homoptera, 3 species in Lepidoptera, 2 species in Coleoptera, 1 species in Orthoprera, 1species in Hemiptera, 1 spedies in Acarina, and 1 species in Stylommatophora. Especially, Aphis gossypii Glover (Aphididae), Anomis megogona Walker(Noctuidae) and Tetranychus urticae Koch (Tetranychidae) were very important species because of their increasing daminge. The highest donsities were observed from May to June in August in Tetranychus urticae. As the enemies and ento-mopathogens of insect pests on Hibiscus syriacus, 1 species of bacteria, 3 species of fungi, 1 species of fungi, 1 species of Hemiptera, 1 species of Coleoptera, 2 species of Hymenopetera, 2 species of Diptera, and 1 species of Acarina were investigated. As the predators and parasitoids of Aphis gossypii, Aphidoletes aphidoletes aphidimyza Rondani (Cecidomyiidae), Meliscaeva cinctella Zetterstedt (Syrphidae), Harmonia axyridis Pallas (Coccinellidae), and Aphidius gifuensis Ashmead (Braconidae), entomopathogenic fungi, Vericillium lecani naturalis strain (Moniliaceae) and Beauveria bassiana naturalis strain strain (Hypocreaceae) were observed and Bacillus thuringiensis naturalis strain (Bacillaceae), B. bassiana, Metarhizium anisopliae naturalis strain (Hypocreaceae), predators of Tetranychus urticae, Amblyseius sp. (Phytoseiidae), and Orius sp. (Anthocoridae) were observed.