• Journal of the Korean Association of Geographic Information Studies
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• v.16 no.3
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• pp.25-39
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• 2013

In this study, the change of the discharge according to the land cover change which acts as one of dominant factors for the outlook of future discharge was analyzed using SWAT(Soil and Water Assessment Tool) model for Yongdam and Daecheong Dam Watershed in the Geum River Basin. The land cover maps generated by Landsat TM satellite images in the past 1990 and 1995 were used as observed data to simulate the land cover in 2000 by CA-Markov serial technique and after they were compared and verified, the changes of land cover in 2050 and 2100 in the future were simulated. The discharge before and after the change of land cover by using input data of SWAT model was compared and analyzed under the A1B scenario. As a result of analyzing the trend in the elapses of year on the land cover in the Geum River Basin, the forest and rice paddy class area steadily decreased while the urban, bare ground and grassland classes increased. As a result of analyzing the change of discharge considering the future change of the land cover, it appeared that the discharge considering the change of land cover increases by 1.83~2.87% on the whole compared to the discharge not considering the change of land cover.

• Korean Journal of Environmental Agriculture
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• v.21 no.3
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• pp.216-222
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• 2002

In order to evaluate the antifungal activity of bacteria against plant pathogenic fungi, 8 bacteria were isolated from mushroom compost hot spring, seaweed, and forest soil and mutants from them were induced by $LD_{95}$ gamma radiation($^{60}Co$). Bacillus circulans K1, Burkholderia gladioli K4 and Bacillus subtilis YS1 showed wide antifungal spectrum against 12 kinds of plant pathogenic fungi. From the radiation sensitivity test, B. gladioli K4 was very sensitive to gamma radiation and its $D_{10}$ value was 0.11 kGy. Antifungal activities of B. circulans Kl-1004 and B. subtilis YS1-1009, which were induced by the radiation of $^{60}Co$ increased against Botryosphaeria dothidea. The mutant strains, B. subtilis YS1-1006 and B. subtilis YS1-1009 were resistant to tebuconazole and copper hydroxide. SAR535, SAR5108, and SAR5118 mutated from Streptomyces sp. SAR01 were antifungal activity deficient mutants against 5 kinds of plant pathogenic fungi compared to wild strain, so that they could be supposed to be model strains far studying antifungal mechanism. It is suggested that various functional types of mutants could be induced by gamma radiation and applied usefully.

• Journal of Korean Society on Water Environment
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• v.23 no.6
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• pp.897-905
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• 2007

Approximately 70% of Korea is composed of forest areas. Especially 48% of agricultural field is practiced at highland areas over 400 m in elevation in Kangwon province. Over 90% of highland agricultural farming is located at Kangwon province. Runoff characteristics at the mountainous area such as Kangwon province are largely affected by steep slopes, thus runoff estimation considering field slopes needs to be utilized for accurate estimation of direct runoff. Although many methods for runoff estimation are available, the Soil Conservation Service (SCS), now Natural Resource Conservation Service (NRCS), Curve Number (CN)-based method is used in this study. The CN values were obtained from many plot-years dataset obtained from mid-west areas of the United States, where most of the areas have less than 5% in slopes. Thus, the CN method is not suitable for accurate runoff estimation where significant areas are over 5% in slopes. Therefore, the CN values were adjusted based on the average slopes (25.8% at Doam-dam watershed) depending on the 5-day Antecedent Moisture Condition (AMC). In this study, the CN-based Long-Term Hydrologic Impact Assessment (L-THIA) direct runoff estimation model used and the Web-based Hydrograph Analysis Tool (WHAT) was used for direct runoff separation from the stream flow data. The $R^2$ value was 0.65 and the Nash-Sutcliffe coefficient value was 0.60 when no slope adjustment was made in CN method. However, the $R^2$ value was 0.69 and the Nash-Sutcliffe value was 0.69 with slope adjustment. As shown in this study, it is strongly recommended the slope adjustment in the CN direct runoff estimation should be made for accurate direct runoff prediction using the CN-based L-THIA model when applied to steep mountainous areas.

• Horticultural Science & Technology
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• v.31 no.6
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• pp.657-665
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• 2013

A large patch disease caused by Rhizoctonia solani AG2-2 (IV) is a serious problem in Korean lawngrass (Zoysia japonica) sites including golf courses and sports fields in Korea. Antagonistic microorganisms against R. solani AG2-2 (IV) were isolated from various forest and crop soil sources in Southern Korea. Among the 61 isolates, I-009, FRIN-001-1, and YPIN-022 strains showing dramatic inhibition of the mycelial growth of R. solani AG2-2 (IV) in the pairing culture were selected as the most potential antagonistic microorganisms for this study. Based on the 16s RNA sequence comparison, I-009 and FRIN-001-1 isolates were identified as Bacillus spp., while YPIN-022 isolate belongs to the genus Pseudomonas. The greater inhibition (clear) zone between two edges of the selected and pathogenic microbes ranged from 11 to 15 mm in three selections, but the others averaged to 7 mm out of 30 mm distance. In another antifungal test using culture filtrate, those three isolates represented a range of 51.7 to 63.5% suppression potential. The selected isolates also inhibited significantly the stem-segment colonization by R. solani AG2-2 (IV) in vivo test by 28.1%, 43.0%, and 23.7% when inoculated with I-009, FRIN-001-1, and YPIN-022, respectively. The highest antagonistic activity for the large patch disease was demonstrated by the isolate FRIN-001-1, which will be useful for developing a bio-pesticide against Rhizoctonia.

• Journal of Korea Water Resources Association
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• v.40 no.6 s.179
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• pp.459-468
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• 2007

To estimate the groundwater recharge, the fully distributed parameter based model, MIKE SHE was applied to the Gyeongan-cheon watershed which is one of the tributaries of Han River Basin, and covers approximately $260km^2$ with about 49 km main stream length. To set up the model, spatial data such as topography, land use, soil, and meteorological data were compiled, and grid size of 200m was applied considering computer ability and reliability of the results. The model was calibrated and validated using a split sample procedure against 4-year daily stream flows at the outlet of the watershed. Statistical criteria for the calibration and validation results indicated a good agreement between the simulated and observed stream flows. The annual recharges calculated from the model were compared with the values from the conventional groundwater recession curve method, and the simulated groundwater levels were compared with the observed values. As a result, it was concluded that the model could reasonably simulate the groundwater level and recharge, and could be a useful tool for estimating spatially/temporally the groundwater recharges, and enhancing the analysis of the watershed water cycle.

• Korean Journal of Environmental Agriculture
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• v.26 no.4
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• pp.313-318
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• 2007

In greenhouse farming, a variety of humic acids have been applied to improve soil conditions and plant growth. However, it is still unclear that how humic acids combined with chemical fertilizers affect growth and quality of fruit vegetable crops. This study was conducted to determine the combination effect of humic acids and chemical fertilizers on the growth and fruit quality of tomato (Lycopersicon esculentum MILL.) grown under greenhouse conditions. Three different formulation types of humic acid were used: liquid type A, liquid type B and solid type C. The tomato plants were grown in three treatment combination plots and in conventional fertilizer (CF) plot with recommended levels of nitrogen, phosphorus and potassium: HA combined with CF (HA+CF), HB combined with CF (HB+CF) and HC combined with CF (HC+CF). For most of growth characteristics (i.e. leaf number, internode length, maximum leaf length, leaf width and chlorophyll contents) determined in this experiment, no significant differences were observed between all combination treatments and CF. However, integrated fruit qualities (i.e. averaged weight, sugar contents and acidity) were slightly improved in the humic acid combined with CF treatments when compared with CF alone treatment. No phytotoxicity was observed with humic acid treatments. However, further studies will probably be needed to use widely and safely these humic acids, in order to ensure a maximizing growth, fruit yield and quality of tomato.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.14 no.6
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• pp.17-27
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• 2011

Prunus serrulata var. serrulata f. spontanea (E. H. Wilson) C. S. Chang was treated with dried swine excrement at various concentration levels, and their growth reactions and the contents of total kjeldahl nitrogen and total phosphoric acid were analyzed. The result is as follows; 1. When the plants were treated with 0.25% of dried swine excrement, the seed germination rate of Prunus serrulata var. serrulata f. spontanea was higher than that of the control. However, it showed a tendency to decrease when the treatment concentration got higher than 0.25%. 2. The growth rate of species was highest when they were treated with 0.25% of dried swine excrement. It tended to decrease with higher treatment concentration than 0.25%. Significant difference was clear between the control and the experimental plants of Prunus serrulata var. serrulata f. spontanea. 3. The contents of nitrogen and phosphoric acid in Prunus serrulata var. serrulata f. spontanea also got higher as the concentration of dried swine excrement increased. However, the content of total kjeldahl nitrogen was highest in the leaves, and followed by the roots and stems. In contrast, the content of total phosphoric acid were highest in the roots, and followed by the leaves and stems. Therefore, stems had the lowest contents of both nitrogen and phosphoric acid. 4. The contents of total kjeldahl nitrogen and total phosphoric acid in the soil were measured before and after the growth experiment of Prunus serrulata var. serrulata f. spontanea treated with dried swine excrement. The contents of nitrogen and phosphoric acid significantly decreased after the experiment. In conclusion, the contents of total kjeldahl nitrogen and total phosphoric acid accumulated in the plants increased as the concentration level of dried swine excrement got higher. The seed germination and plant growth rates were best at 0.25% treatment of dried swine excrement. The treatment of dried swine excrement may bring high effects on increasing the plant growth rate but could damage the plants with higher concentration than they need. Different optimal concentration levels of dried swine excrement for different plants should be found before it is used as fertilizer.

• Korean Journal of Environmental Agriculture
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• v.12 no.3
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• pp.219-229
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• 1993

Effects of paper mill sludge-fertilizers were investigated on the growth performances of tree seedlings under field conditions. Two types of sludge-fertilizers applied were an organic sludge compost and a processed sludge-pellet fertilizer strengthened with several components of organic and inorganic nutrients. Three species of tree seedlings studied were ibota privet(Ligustrum obtusifolium) and yellow poplar(Liriodendron tulipifera) of 1-0 year seedlings and also eastern white pine(Pinus strobus) of 2-2 year seedlings. Nitrogen and phosphorus contents of plot soil treated with sludge compost+sludge-pellet fertilizer+sludge-pellet fertilizer plot were increased to 8 times and 2.6 times as those of control plot, respectively. The growth performances of root collar diameter and seedling height were repeatedly measured up to five times through the growing season and the results were analyzed statistically by analysis of variance of randomized block design and Duncan's multiple range test. The growth performances of dry weight were measured after last fifth measurements.

• Journal of Ginseng Research
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• v.30 no.1
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• pp.31-40
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• 2006

Ginsengs(1-3 years old) from the Keumsan were analysed for the rare earth element(REE) contents and compared with their soils from the biotite granite(CR), phyllite(PH) and shale(SL) areas. In the soils, high REE contents and correlations were found in the SL. In the ginsengs, high element contents were shown in the SL. High correlations were found in the 3 year. In the upper parts, the 2 year of the GR was mainly high. Comparing with the same aged ginsengs, high elements were shown in the SL. Positive correlations were dominated and high correlations were shown in the 3 year ginsengs. In the root parts, the GR was high in the 2 year while the PH and SL were high in the 3 year. Comparing with the same ages, high elements were shown in the SL. High correlation relationships were found. Comparing between upper and root parts, the upper parts were mainly high, LREE showed big differences and relative ratios of the 2 year were mainly high. Comparing between soils and ginsengs, the soils were mainly high. Ratios between soils and root parts(soils/root parts) were higher than those of the upper parts. Ratios of the LREE showed big differences relative to those in the HREE and the ratios increased with ages. Overall results suggested that ginsengs of the SL were similar to those of soils and those of the PH showed big differences.

• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.1951-1964
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• 2016

1,4-Dioxane-degrading bacterial consortia were enriched from forest soil (FS) and activated sludge (AS) using a defined medium containing 1,4-dioxane as the sole carbon source. These two enrichments cultures appeared to have inducible tetrahydrofuran/dioxane and propane degradation enzymes. According to qPCR results on the 16S rRNA and soluble di-iron monooxygenase genes, the relative abundances of 1,4-dioxane-degrading bacteria to total bacteria in FS and AS were 29.4% and 57.8%, respectively. For FS, the cell growth yields (Y), maximum specific degradation rate ($V_{max}$), and half-saturation concentration ($K_m$) were 0.58 mg-protein/mg-dioxane, $0.037mg-dioxane/mg-protein{\cdot}h$, and 93.9 mg/l, respectively. For AS, Y, $V_{max}$, and $K_m$ were 0.34 mg-protein/mg-dioxane, $0.078mg-dioxane/mg-protein{\cdot}h$, and 181.3 mg/l, respectively. These kinetics data of FS and AS were similar to previously reported values. Based on bacterial community analysis on 16S rRNA gene sequences of the two enrichment cultures, the FS consortium was identified to contain 38.3% of Mycobacterium and 10.6% of Afipia, similar to previously reported literature. Meanwhile, 49.5% of the AS consortium belonged to the candidate division TM7, which has never been reported to be involved in 1,4-dioxane biodegradation. However, recent studies suggested that TM7 bacteria were associated with degradation of non-biodegradable and hazardous materials. Therefore, our results showed that previously unknown 1,4-dioxane-degrading bacteria might play an important role in enriched AS. Although the metabolic capability and ecophysiological significance of the predominant TM7 bacteria in AS enrichment culture remain unclear, our data reveal hidden characteristics of the TM7 phylum and provide a perspective for studying this previously uncultured phylotype.