• Analytical Science and Technology
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• v.27 no.3
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• pp.147-152
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• 2014

RUVIS(Reflective Ultraviolet Imaging System) is an effective equipment that detects the location of latent fingerprint at crime scene using short wavelength ultraviolet of 254 nm. In this study, the degree of DNA damage in biological samples was compared depending on the distance and time of processing using four commonly used RUVIS. 50% of DNA was damaged by treating 10 seconds at 10 cm distance in 3 types of RUVIS such as Police RUVIS, SIRCHIE mini light and SIRCHIE RUVIS. In addition, the degree of DNA damage was increased as the distance was closer and the treatment time was longer. It showed that short wavelength UV could cause DNA damage when used close to the samples at crime scene. Therefore, it was suggested to use RUVIS at a distance of at least 1 m. The degree of DNA damage was not significant by Polilight which used long wavelength ultraviolet of 350 nm. As a result, the choice and usage of which UV light and RUVIS were critical for detection of fingerprint and successful DNA typing.

• Analytical Science and Technology
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• v.30 no.6
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• pp.312-318
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• 2017

The shelf life of 1,2-indandione/zinc (1,2-IND/Zn) solution and polyvinylpyrrolidone (PVP) solution, which are known as reagents for developing latent fingermarks deposited on the surface of thermal paper, was studied. The standard latent fingermarks used for comparisons were artificial latent fingermarks printed on thermally sensitive and non-sensitive surfaces with the same intensity. Upon treatment of standard latent fingermarks with the pre-mixed 1,2-IND/Zn and PVP solutions, the fingermarks could be successfully developed until 3 days after the preparation of the mixture. However, from the third day after mixing the reagents, blackening was observed on the surface of the thermal paper, indicating deterioration of the reagent performance. The 1,2-IND/Zn and PVP solutions separately stored without mixing in advance were mixed immediately before use, and the development efficiency of the latent fingermarks deposited on the surface of thermal paper was observed. The performance of the PVP solution decreased after 20 days from the preparation of the reagent. It was also found that the shelf life of 1,2-IND/Zn and PVP mixture was determined by the PVP solution. The effect of oxygen and moisture on the degradation of PVP was investigated. It was found that the performance of the PVP solution deteriorated because of the influence of moisture, though it was not affected by oxygen.

• Analytical Science and Technology
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• v.31 no.1
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• pp.47-56
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• 2018

Finding the blood left at a crime scene is very important to reconstruct or solve a criminal case. Although numerous reagents have been developed for use at crime scenes, luminol is the most representative. Bluestar Forensic has been used in recent years, but is expensive and cannot be stored after preparation. This study aims to develop a new luminol reagent that can be stored for a long period of time while maintaining the chemiluminescence intensity at the level of Bluestar Forensic. Because luminol dissolves well in aqueous alkaline solutions, the use of sodium hydroxide in the preparation of luminol reagents can promote the decomposition of hydrogen peroxide. Magnesium sulfate, sodium silicate, and potassium triphosphate have been used as hydrogen peroxide stabilizers. The effects of the addition of these substances on the chemiluminescence emission intensity and the storage period of the luminol reagents were confirmed. The addition of a hydrogen peroxide stabilizer was shown to have no significant affect on the chemiluminescence emissions intensity or stabilized pH of the luminol reagent during storage. It also greatly increases the shelf life of the reagents. The use of magnesium sulfate as a hydrogen peroxide stabilizer is the most appropriate. When sodium perborate is used instead of hydrogen peroxide as an oxidizing agent, there is no significant change in the sensitivity and chemiluminescence emissions intensity, but the storage period is shortened. However, after the reaction with blood, the pH of the mixed solution does not increase significantly, and is judged to be more suitable than a reagent made of hydrogen peroxide.

• Analytical Science and Technology
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• v.13 no.2
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• pp.1027-1048
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• 2000

• MALSORI
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• no.46
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• pp.25-35
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• 2003

This study deals with the disguised voice (or voice disguise) in the field of forensic phonetics. We especially studied the effects of the methods of disguised voice on the aural decision. Within the nonelectronic-deliberate voice disguise area, the methods of disguised voice include use of lowered pitch, pinched nostrils, falsetto, and whisper. Ten (male:5, female:5) Seoul speakers made a recording of 16 sentences. In the aural test, 30 subjects listened normal and disguised voice. And they were asked to make a decision whether speakers identified or not. The result is as follows: The speaker verification of the falsetto and whisper was more difficult than the lowered pitch and pinched nostrils.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2011.05a
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• pp.455-458
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• 2011

일명 일심회 사건으로 디지털 포렌식이 과학수사에서 활용되는 가운데 판결한 최근 사례로서 1심 판결과 2심 판결에서 디지털 증거의 채택여부를 달리하였다. 학계와 법조계의 의견이 분분한 가운데 일심회 판결문의 1심 판결에 대한 중요성은 수차례에 걸쳐 연구되고 논의되었으나 2심 판결에서 이를 번복하므로 디지털 증거의 인정여부를 위한 문제점과 해결, 절차에 대한 연구가 다시 시작할 시점에 이르렀다. 본 연구에서는 디지털 증거가 법적증거로 인정될 수 있는가에 대한 논의를 시작으로, 일심회 사건의 1심과 2심의 판결을 중심으로 디지털 포렌식 증거의 분석을 통해서 문제점과 해결방안을 제시한다. 본 연구결과 디지털 포렌식의 수사현장에서 필요한 조건을 검토하고, 이를 이행함으로써 과학수사의 일환으로 디지털 증거가 법정에서 채택할 수 있도록 한다.

• Analytical Science and Technology
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• v.35 no.2
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• pp.82-91
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• 2022

Grayanotoxin-contaminated honey exhibits toxicity. In this study, a reliable and sensitive liquid-chromatography-tandem-mass-spectrometric method (LC-MS/MS) was developed and validated for the quantitation of grayanotoxin I and grayanotoxin III in honey. The grayanotoxins were extracted from honey via solid phase extraction and separated on a biphenyl column with a mobile phase consisting of 0.5 % acetic acid in water and methanol. Mass spectrometric detection was performed in the multiple-reaction monitoring mode with positive electrospray ionization. The calibration curve covered the range 0.25 to 100 ㎍/g. The intra- and inter-day deviations were less than 10.6 %, and the accuracy was between 94.3 and 114.0 %. The validated method was successfully applied to the determination of grayanotoxins in mad honey from Nepal. The concentrations of grayanotoxin I and grayanotoxin III in 33 out of 60 mad honey samples were 0.75 - 64.86 ㎍/g and 0.25 - 63.99 ㎍/g, respectively. The method established herein would help in preventing and confirming grayanotoxin poisoning.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.17 no.2
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• pp.591-608
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• 2023

With the emergence of advanced encryption technologies such as Quantum Cryptography and Full Disk Encryption, an era of strengthening information security has begun. Users respond positively to the advancement of privacy-enhancing technology, on the other hand, investigative agencies have difficulty unveiling the actual truth as they fail to decrypt devices. In particular, unlike past ciphers, encryption methods using biometric information such as fingerprints, iris, and faces have become common and have faced technical limitations in collecting digital evidence. Accordingly, normative solutions have emerged as a major issue. The United States enacted the CLOUD Act with the legal mechanism of 'Contempt of court' and in 2016, the United Kingdom substantiated the Compelled Decryption through the Investigatory Powers Act (IPA). However, it is difficult to enforce Compelled Decryption on individuals in Korea because Korean is highly sensitive to personal information. Therefore, in this paper, we sought a method of introducing a Compelled Decryption that does not contradict the people's legal sentiment through a perception survey of 95 people on the Compelled Decryption. We tried to compare and review the Budapest Convention with major overseas laws such as the United States and the United Kingdom, and to suggest a direction of legislation acceptable to the people in ways to minimize infringement of privacy. We hope that this study will be an effective legal response plan for law enforcement agencies that can normatively overcome the technical limitations of decoding.

• Imaging Science in Dentistry
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• v.49 no.1
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• pp.19-26
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• 2019

Purpose: It has been proposed that using new prediction methods, such as neural networks based on dental data, could improve age estimation. This study aimed to assess the possibility of exploiting neural networks for estimating age by means of the pulp-to-tooth ratio in canines as a non-destructive, non-expensive, and accurate method. In addition, the predictive performance of neural networks was compared with that of a linear regression model. Materials and Methods: Three hundred subjects whose age ranged from 14 to 60 years and were well distributed among various age groups were included in the study. Two statistical software programs, SPSS 21 (IBM Corp., Armonk, NY, USA) and R, were used for statistical analyses. Results: The results indicated that the neural network model generally performed better than the regression model for estimation of age with pulp-to-tooth ratio data. The prediction errors of the developed neural network model were acceptable, with a root mean square error (RMSE) of 4.40 years and a mean absolute error (MAE) of 4.12 years for the unseen dataset. The prediction errors of the regression model were higher than those of the neural network, with an RMSE of 10.26 years and a MAE of 8.17 years for the test dataset. Conclusion: The neural network method showed relatively acceptable performance, with an MAE of 4.12 years. The application of neural networks creates new opportunities to obtain more accurate estimations of age in forensic research.

• Biomedical Science Letters
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• v.26 no.4
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• pp.288-295
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• 2020

Infection of Helicobacter pylori on gastric mucosa is associated with various gastric diseases. According to the WHO, H. pylori causes gastric cancer and has been classified as a class I carcinogen. Riboflavin is an essential vitamin which presents in a wide variety of foods. Previous studies have shown that riboflavin/UVA was effective against the growth inhibition of Staphylococcus aureus, S. epidermidis and multidrug-resistant Pseudomonas aeruginosa and had the potential for antimicrobial properties. Thus, we hypothesized that riboflavin has a potential role in the growth inhibition of H. pylori. To demonstrate inhibitory concentration of riboflavin against H. pylori, we performed agar and broth dilution methods. As a result, we found that riboflavin inhibited the growth of H. pylori. The MIC was 1 mM in agar and broth dilution test. Furthermore, to explain the inhibitory mechanism, we investigated whether riboflavin has an influence on the replication-associated molecules of the bacteria using RT-PCR to detect mRNA expression level in H. pylori. Riboflavin treatment of H. pylori led to down-regulation of polA and dnaB mRNA expression levels in a dose dependent manner. After then, we also confirmed whether riboflavin has cytotoxicity to human cells. We used AGS, a gastric cancer cell line, and treated with riboflavin did not show statistically significant decrease of cell viability. Thus, these results indicate that riboflavin can suppress the replication machinery of H. pylori. Taken together, these findings demonstrate that riboflavin inhibits growth of H. pylori by inhibiting replication of the bacteria.