• 한국초지조사료학회지
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• 제16권4호
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• pp.260-266
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• 1996

To find out the residual effect of phosphate fertilization level on the dry matter yield and nutrient plloductivity of pasture plants, this experiment was arranged as a randomized complete block design with six treatments(0-0, 50-50, 100-65, 150-65, 200-65 and 250-65kg $P_2O_5$), those were composed of three $P_2O_5$ fertilization level(0,50 and 65kg $P_2O_5$ after phosphate fertilization trial with six treatments(0, 50, 100, 150, 200 and 250kg $P_2O_5$/ha) from 1989 to 1992, and conducted at hilly land in Kwangju, Kyonggi Province from 1993 to 1994. The results obtained are summarized as follows : As the available phosphate of soil increase, the early growth and cover degree of pasture plants wintered were more favorable. With 150-65kg $P_2O_5$ application for two years, the average dry matter yield of grasses, 9,862kglha was similared to that of 200-65kg $P_2O_5$/ha. The crude protein and energy productivity of pasture plants tended to increase as available phosphate of soil was increased, but those were not different between 150-65 and 250-65kg P 2 0 4 a Efficiency of $P_2O_5$ was highest at $P_2O_5$ 150-65kg/ha fertilization, wHich produced dry matter 11 1.8kg. net energy lactation(NEL) 67 1.2M.f per I kg P205. Therefore, phosphate fertilization is recommended strongly application with 150kg/ha for three years after pasture establishment, and then 65kg/ha for two years a reasonabl management in hilly grassland.

• 한국초지조사료학회지
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• 제12권1호
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• pp.1-5
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• 1992

자연군락에 인산비과를 시용하여 토양용액중의 인산농도를 높일 경우, 일년생 및 다년생식물의 초장, 건물량의 반응성을 조사하였다. 또한, Italian ryegrass를 자연군락중에 파종하여, 그 후의 정착과 성장에 미치는 인산비과의 영향을 조사함으로서 일년생식물과 다년생식물과의 경쟁관계에 미치는 토양중 인산 농도의 영향을 검토하였다. 다년생식물인 MIscanthus, SaSa 및 Artemisia군락에서는 초장, 건물량에 대한 인산 시비의 효과는 작었지만, 일년생식물인 Polygonum 및 Echinochloa군락에서는 효과가 크게 나타났다. 각 자연군락내의 Italian ryegrass의 초장, 밀도 및 지상부 건물량은 토양의 전인산과 유효태 인산 수준에 크게 의존하였고, 군락중 상대광양자밀도에는 의존도가 낮았다.

• 한국초지조사료학회지
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• 제9권2호
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• pp.77-81
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• 1989

This experiment was conducted to elucidate the changes of dry matter production in permanent pasture and its relation with relative yield of the 1st cutting, regrowth capacity, and persistance of orchardgrass sward, based on the data of preceeding The results were summarized as follows; 1. Relationship between relative yield of the 1st cutting and regrowth capacity were changed by the times of established pasture. Thus, the relative yield of the 1st cutting have negative significant correlation (P < -0.001) with regrowth capacity. 2. Regrowth capacity(RC) was positively significant correlations with the dry weight of plant (DW/pl.), number of tillers per plant (NT/pl.), plant size(PS) and distance between adjacent plants (DIS) of the 1st cutting. Also, regrowth capacity(RC) was positively correlations with the dry weight of plant (DW/pl.), number of tillers per plant (NT/pl.), stubble diameter(DIA), stubble area(SB), plant size(PS) and distance between adjacent plants(D1S) of the 2nd cutting. 3. The coefficients of regression of the 1st cutting showed a tendency to slightly decrease, on the other hand, the 2nd cutting and total yield were tended to increase by the times of established pasture. 4, Between the regrowth capacity and persistency were may deeply concerned to the changes of dry matter production in permanent pasture. 5. The process of dry matter production in permanent pasture can be shown in following diagrams.

• 한국초지조사료학회지
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• 제18권3호
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• pp.267-272
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• 1998

5품종의 오차드그래스 종자로부터 직접 캘러스를 유도하고, 형성된 캘러스로부터 식물체를 재분화하는 조건을 확립하였다. 공시품종증 합성 19호가 캘러스 유도 및 재분화에서 가장 우수한 것으로 판명되었으며, SH, MS, N6 배지주에서 캘러스 유도시에는 MS 배지가, 재분화시에는 N6 배지가 유리한 것으로 나타났다. 각 단계별 배지중 호르몬 첨가는 캘러스 유도 및 증식시에 dicamba $3\;mg/\;{\ell}$, 뿌리와 잎의 유도시에 NAA $1\;mg/\;{\ell}$와 kinetin $5\;mg/\;{\ell}$, 재분화 완성기에 무첨가한 조건이 가장 효율이 좋았으며, 캘러스로부터 완전한 식물체로 재분화되는데 필요한 시간은 약 50~80일이었다.

• 한국초지조사료학회지
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• 제15권4호
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• pp.259-264
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• 1995

Several benefits provided by white clover (Tnifoliurn repens L.) can be elevated as relationship between its morphological and physiological characteristics is clarified. The experiment was done to analyze the relationship between them in the clover with different leaf size. Individual plants of Osceola (large leaf), Grasslands Huia (medium leaf) and Aberystwyth S184 (small leaf) were grown in 15m plastic pot containing a 1:2:1 soil:sand:Promix mixture for 55 days, and then clipped to remove all fully expanded leaves every 7, 14 or 28 days. To measure the cultivar response, plants were sampled for morphological and physiological parameters on the date (0) after final harvest and 1, 3, 7, 14 and 28 days after the final harvest and then their relationship was analyzed. Osceola had greater leaf area per trifoliolate but less no. of leaves per plant to result in similar total leaf area per plant of all cltivars. Stolon length and no. of growing tips per plant declined with smaller leaf size although the result in biomass was reverse. Stolon of all cultivars showed the greatest fluctuation in total nonstructud carbohydrate during the regrowing period but nitrogen concentrations of all their fractions and dinitrogen fixation did similar patterns. Stolon and root of Osceola, moreover, were the highest ones. Biomass, stolon length and total leaf area per plant of all cultivars were positively correlated to carbohydrate concentration of all frauions and dinitrogen fixation. In Osceola relationship between nitrogen concentration of stolon and the characters showed in Osceola was positively done.

• 한국초지조사료학회지
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• 제36권3호
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• pp.243-247
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• 2016

Arsenic (As) is a toxic element that easily taken up by plants root. Several toxic forms of As disrupt plant metabolism by a series of cellular alterations. In this study, we applied annealing control primer (ACP)-based reverse transcriptase PCR (polymerase chain reaction) technique to identify differentially expressed genes (DEGs) in alfalfa roots in response to As stress. Two-week-old alfalfa seedlings were exposed to As treatment for 6 hours. DEGs were screened from As treated samples using the ACP-based technique. A total of six DEGs including heat shock protein, HSP 23, plastocyanin-like domain protein162, thioredoxin H-type 1 protein, protein MKS1, and NAD(P)H dehydrogenase B2 were identified in alfalfa roots under As stress. These genes have putative functions in abiotic stress homeostasis, antioxidant activity, and plant defense. These identified genes would be useful to increase As tolerance in alfalfa plants.

• 한국초지조사료학회지
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• 제9권1호
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• pp.15-19
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• 1989

This experiment was carried out in order to know the changes in dry matter production of the 2nd cutting by the times of established pasture and its relation with morphological and structural characters of orchardgrass sward. Experimental pasture was established by the times of year, from 1 year to 7 year, 1986 through 1980, alternately. The results were summarizedas follows: 1. The population density of the 2nd cutting was extremely decreased by the times of established year. 2. The dry weight of $plantsW)$ was positively significant correlations with plant length(PL), number of tillers per plant (NT/pl.) and the dry weight of plant(DW/pl.). 3. The highest value of the dry weight of plants(DW) was obtained at 7 year old pasture. 4. The pasture productivity of 7 year old pasture was resulted by the increases of regrowth capacity, yield components and structural characters of the 2nd cutting. 5 . Relationship between morphological and structural characters concerned vegetational structure of the 2nd cutting were changeable year by year.

• 한국초지조사료학회지
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• 제32권4호
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• pp.325-334
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• 2012

It has been known that endophyte (Neotyphodium coenophialum) is beneficial to tall fescue (Schedonorus phoenix Scop.) because the mutualistic endophyte is able to confers tolerance against abiotic and biotic stresses to tall fescue. However, this fungal endophyte produces toxic alkaloid resulting in negative effects on animal performance. Recently, Non-toxic endophyte have been developed and inserted into tall fescue to avoid detrimental effect on animal but remaining positive influence on tall fescue. In order to keep this beneficial impact, it is essential to have endophyte infected tall fescue through vertical transmission from maternal plants to seeds. Little research has been carried out on endophyte transmission. To get basic information related to endophyte transmission, experiment was conducted to examine the effect of plant genotype on endophyte transmission under water stresses. Overall endophyte concentration in seeds was higher than that in panicles and endophyte concentration in seeds and panicles relied on plant. This study revealed that drought is not a critical component to control the endophyte transmission from maternal plants to seeds. Plant genotype is an important factor controlling the endophyte transmission from plant to seed.

• 한국초지조사료학회지
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• 제21권1호
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• pp.21-26
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• 2001

환경 스트레스에 의해 야기되는 활성 산소종에 의한 피해에 내성을 가지는 목초의 개발을 위하여 오차드그래스의 배반 조직 유래의 캘러스에 배추유래의 cytosolic glutathione reductase 유전자(BcGRl)를 Agrobucterium tumefaciens EHA101을 매개로 형질전환시켰다. Hygromcin으로 선발된 캘러스로부터 재분화된 식물체는 야생형과 비교하여 형태적으로 차이를 나타내지 않았다. PCR 및 Southern blot 분석을 통하여 형질전환 식물체의 염색체 내에 BcGRl 유전자가 integration 되었음을 확인하였다. 오차드그래스의 잎으로부터 total RNA를 분리하여 Northern blot 분석을 실시한 결과, 도입된 유전자가 형짙전환 식물체 내에서 지속적으로 발현된다는 것을 확인하였다.

• 한국초지조사료학회지
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• 제42권2호
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• pp.120-126
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• 2022

The objective of this study was to determine effects of phosphorus on lignification and carbohydrate metabolism in Kentucky bluegrass under drought stress. Drought stress was induced by reducing of water to plants in pots. Two types of phosphorus were applied as potassium phosphate (PO₄^3-; P) or potassium phosphonate (PO₃^3-; PA) in drought-stressed plants. Drought had significant negative effects on plant growth, as revealed by reduced biomass of shoot. Drought-induced increase of lignin content was concomitant with the increase of phenylalanine ammonia-lyase (PAL). Soluble sugar content was highly increased but fructan content was largely decreased by drought stress. However, the application of phosphorus was efficient to ameliorate the adverse effects of drought. PA application improved reduced shoot growth and relative water content, and inhibited lignification synthesis with a reduction of PAL activity. P or PA application maintained soluble sugar and fructan content at similar levels to controls under drought stress. These results indicate that phosphorus application may mitigate the drought stress by inhibiting the lignification and promoting the fructan assimilation.