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Effects of Forage Sources on Rumen Fermentation Characteristics, Performance, and Microbial Protein Synthesis in Midlactation Cows

  • Xua, Jun;Houa, Yujie;Yang, Hongbo;Shi, Renhuang;Wu, Caixia;Huo, Yongjiu;Zhao, Guoqi
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.5
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    • pp.667-673
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    • 2014
  • Eight multiparous Holstein cows ($632{\pm}12$ kg BW; $135{\pm}16$ DIM) were used in a replicated $4{\times}4$ Latin square design to evaluate the effects of forage sources on rumen fermentation characteristics, performance, and microbial protein (MCP) synthesis. The forage portion of the diets contained alfalfa hay (AH), oat hay (OH), Leymus chinensis (LC), or rice straw (RS) as the primary source of fiber. Diets were isonitrogenous and isocaloric, and cows were fed four corn silages based total mixed rations with equivalent nonfiber carbohydrate (NFC) and forage neutral detergent fiber (NDF). Dry matter intake was not affected by the source of dietary forages, ranging from 18.83 to 19.20 kg/d, consequently, milk yield was similar among diets. Because of the numerical differences in milk fat and milk protein concentrations, 4% FCM and ECM yields were unchanged (p>0.05). Mean rumen pH, NH3-N content, and concentrations of volatile fatty acids in the rumen fluid were not affected by the treatments (p>0.05). Dietary treatments did not affect the total tract apparent digestibility of dry matter, organic matter, and crude protein (p>0.05); however, digestibility of NDF and acid detergent fiber in RS diet was higher compared with AH, OH, and LC diets (p<0.05). Total purine derivative excretion was higher in cows fed AH, OH, and LC diets compared with those fed RS diet (p<0.05), consequently, estimated MCP synthesis was 124.35 g/d higher in cows fed AH diet compared with those fed RS diet (p<0.05). The results indicated that cows fed AH, OH, LC, and RS diets with an equivalent forage NDF and NFC have no unfavourable effect on the ruminal fermentation and productive parameters.

Comparison of Forage Productivity and Outbreak Rate Between Corn Varieties in Rice Black-Streaked Dwarf Virus (RBSDV) Prevalent Area (검은줄오갈병 발생이 심한 지역에서 옥수수 품종의 발병률과 사초 생산성 비교)

  • Choi, Gi Jun;Lim, Young Chul;Yoon, Sei Hyung;Ji, Hee Chung;Lee, Sang-Hoon;Jung, Min Woong;Seo, Sung;Park, Hyung Soo;Kim, Ki-Yong
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.33 no.2
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    • pp.111-116
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    • 2013
  • This experiment was carried out to select a resistant corn variety for rice black-streaked dwarf virus (RBSDV) disease in a RBSDV prevalent area (Gochang of Jeollabukdo) from 2006 to 2008. Ten corn varieties for silage preparation were cultivated with first cropping and second cropping system in an RBSDV field, and were tested outbreak rates of RBSDV and dry matter (DM) yield of forage. The outbreak rates of RBSDV were significantly different between corn varieties. Therefore, the resistance degree of corn varieties for RBSDV were divided into 4 groups; very resistant ('Kwangpeyongok' and 'Kwanganok'), resistant ('P3156' and 'P3394'), mildly resistant ('Cheonganok' and 'P32P75') and susceptible ('Suwon19', 'DK697' and 'GW6959') groups respectively. DM yield of forage in RBSDV fields exhibited comparatively significant differences between varieties (p<0.05). DM yield of resistant varieties ('Kwangpeyongok', 'Kwanganok', 'P3156' and 'P3394'), were higher (14~26%) than those of susceptible varieties ('Suwon 19', 'DK697' and 'GW6959'). Therefore, resistant varieties were recommended for increasing forage productivity in field of RBSDV prevalent areas.

Effect of Defaunation on In Vitro Fermentation Characteristics and Methane Emission When Incubated with Forages

  • Qin, Wei-Ze;Choi, Seong-Ho;Lee, Seung-Uk;Lee, Sang-Suk;Song, Man-Kang
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.33 no.3
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    • pp.197-205
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    • 2013
  • An in vitro study was conducted to determine the effects of defaunation (removal of protozoa) and forage sources (rice straw, ryegrass and tall fescue) on ruminal fermentation characteristics, methane ($CH_4$) production and degradation by rumen microbes. Sodium lauryl sulfate, as a defaunation reagent, was added into the mixed culture solution to remove ruminal protozoa at a concentration of 0.375 mg/ml. Pure cellulose (0.64 g, Sigma, C8002) and three forage sources were incubated in the bottle of culture solution of mixed rumen microbes (faunation) or defaunation for up to 24 h. The concentration of ammonia-N was high under condition of defaunation compared to that from faunation in all incubations (p<0.001). Total VFA concentration was increased at 3, 6 and 12 h (p<0.05~p<0.01) but was decreased at 24 h incubation (p<0.001) under condition of defaunation. Defaunation decreased acetate (p<0.001) and butyrate (p<0.001) proportions at 6, 12 and 24 h incubation times, but increased propionate (p<0.001) proportion at all incubation times for forages. Effective degradability of dry matter was decreased by defaunation (p<0.001). Defaunation not only decreased total gas (p<0.001) and $CO_2$ (p<0.01~0.001) production at 12 and 24 h incubations, but reduced $CH_4$ production (p<0.001) at all incubation times for all forages. The $CH_4$ production, regardless of defaunation, in order of forage sources were rice straw > tall fescue > ryegrass > cellulose (p<0.001) up to 24 h incubation.

The Main Suppressing Factors of Dry Forage Intake in Large-type Goats

  • Thang, Tran Van;Sunagawa, Katsunori;Nagamine, Itsuki;Kishi, Tetsuya;Ogura, Go
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.3
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    • pp.341-352
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    • 2012
  • In large-type goats that were fed on dry forage twice daily, dry forage intake was markedly suppressed after 40 min of feeding had elapsed. The objective of this study was to determine whether or not marked decreases in dry forage intake after 40 min of feeding are mainly caused by the two factors, that is, ruminal distension and increased plasma osmolality induced thirst produced by dry forage feeding. Six large-type male esophageal- and ruminal-fistulated goats (crossbred Japanese Saanen/Nubian, aged 2 to 6 years, weighing $85.1{\pm}4.89kg$) were used in two experiments. The animals were fed ad libitum a diet of roughly crushed alfalfa hay cubes for 2 h from 10:00 to 12:00 am during two experiments. Water was withheld during feeding in both experiments but was available for a period of 30 min after completion of the 2 h feeding period. In experiment 1, saliva lost via the esophageal fistula was replenished by an intraruminal infusion of artificial parotid saliva (RIAPS) in sham feeding conditions (SFC) control, and the treatment was maintained under normal feeding conditions (NFC). In experiment 2, a RIAPS and non-insertion of a balloon (RIAPS-NB) control was conducted in the same manner as the SFC control of experiment 1. The intraruminal infusion of hypertonic solution and insertion of a balloon (RIHS-IB) treatment was carried out simultaneously to reproduce the effects of changing salt content and ruminal distension due to feed entering the rumen. The results of experiment 1 showed that due to the effects of multiple dry forage suppressing factors when feed boluses entered the rumen, eating rates in the NFC treatment decreased (p<0.05) after 40 min of feeding and cumulative dry forage intake for the 2 h feeding period reduced to 43.8% of the SFC control (p<0.01). The results of experiment 2 indicated that due to the two suppressing factors of ruminal distension and increased plasma osmolality induced thirst, eating rates in the RIHS-IB treatment were, as observed under NFC, reduced (p<0.05) and cumulative dry forage intake for the 2 h feeding period decreased to 34.0% of the RIAPS-NB control (p<0.01). The combined effects of ruminal distension and increased plasma osmolality accounted for 77.5% of the suppression of dry forage intake 40 min after the start of dry forage feeding. The results indicate that ruminal distension and increased plasma osmolality induced thirst are the main factors in the suppression of dry forage intake in large-type goats.

In vitro Rumen Fermentation Patterns of Environment Friendly Whole Crop Barley, Italian Ryegrass and Rice Straw Silages (친환경 청보리, 이탈리안 라이그라스, 볏짚사일리지의 In vitro 반추위 발효성상 비교연구)

  • Mbiriri, David Tinotenda;Oh, Sung-Jin;Lee, A-Reum;Chae, Jung-Il;Choi, Chang-Weon;Choi, Nag-Jin
    • Korean Journal of Organic Agriculture
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    • v.20 no.2
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    • pp.221-230
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    • 2012
  • Rumen fermentative characteristic is useful indicators of the quality of ruminant feed stuffs and diets. An in vitro rumen fermentation experiment was therefore carried out to compare fermentation patterns among three forage sources. These were whole crop barley (WCBS), Italian ryegrass silage (IRGS) and rice straw silages (RSS). Rice straw (RS) was the control, making the treatments 4 in total. Forages were randomly allocated to serum bottles. The incubation times were arranged 0, 3, 6, 9, 12, 24, 48 and 72h at $39^{\circ}C$, respectively. Each forage source was replicated 3 times per incubation time. At each sampling time, total gas and pH were measured, whilst individual volatile fatty acids (VFAs), total volatile fatty acids (TVFAs) and ammonia nitrogen ($NH_3$-N) were determined later after storing samples at $-20^{\circ}C$. Acetate: Propionate ratio (A/P) was then calculated. Forage source had a significant effect (P<0.001) on pH and $NH_3$-N. RSS maintained higher pH values than the rest of the forage sources. A decreasing pH trend with increased time of incubation, in agreement with literature, was observed for all forage sources. WCBS recorded $NH_3$-N values higher than all the other treatments. Total gas, individual and total VFA and A/P ratio were not affected by forage source. However, there was a significant difference in all parameters (p<0.05) among forage sources at sampling periods at 3 to 72h. Therefore, the present results indicating that WCBS, IRGS, RS and RSS maintained in vitro rumen pH above the critical value. Also, WCBS produced the highest NH3-N and on this merit could be of better nutritive value, in vivo, in the ruminant.

Utilization of Sorghum Forage, Millet Forage, Veldt Grass and Buffel Grass by Tswana Sheep and Goats when Fed Lablab purpureus L. as Protein Supplement

  • Aganga, A.A.;Autlwetse, M.N.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.8
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    • pp.1127-1132
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    • 2000
  • Forty yearling Tswana sheep and goats (20 sheep and 20 goats) of both sexes were used in a feeding trial conducted in Botswana College of Agriculture (B.C.A) Content Farm in Gaborone for three months. The animals were randomized into four treatment groups of five animals per species balancing for weight and sex such that average initial weights were not statistically different. The sheep and goats were individually housed and fed under a common roof. All the animals were fed on Lablab purpureus L. as a protein supplement which was 40% of the ration. In addition to L.purpureus L. the control groups of both species were fed on 60% Cenchrus ciliaris L. as basal diet. The other three treatment groups were fed on different forages namely; sorghum forage (Sorghum sudanense (Piper) Stapf), millet forage (Pearl millet, Pennisetum typhoides (Burm.) Stapf and Hubb.) and veldt grass mainly Urochloa mosambicensis (Hack.) as basal diet (60%). Water was provided individually to all the animals on ad lib. basis. Daily intakes of feed and water were recorded and weighing of the animals was done every two weeks. The collected data were analysed statistically for differences. Average daily weight gain by Tswana sheep was significantly different (p<0.05), sheep fed on millet forage had a higher daily weight gain $(120.24{\pm}8.91g)$ compared with sheep fed on veldt grass $(92.86{\pm}6.94g)$. Treatment effects on daily total DM intake by sheep were significant, the control group (C. ciliaris L.) had higher intake $(705.77{\pm}10.22g)$ and those fed on sorghum forage had the least intake $(668.10{\pm}10.70g)$. There was no significant difference (p>0.05) in the average daily weight gain by Tswana goats and it was 84.52, 73.81, 83.33 and 78.57 g for goats fed on C. ciliaris L., sorghum forage, millet forage and veldt grass respectively. Average daily total DM intake by goats was 655.27, 652.64, 650.07 and 650.94 g for C. ciliaris L., sorghum forage, millet forage and veldt grass respectively. Feed conversion efficiency was 8.00, 8.98, 7.93 and 8.34 for goats fed on C. ciliaris L., sorghum forage, millet forage and veldt grass respectively and were not significantly different (p>0.05).

Impact of Different Environmental Conditions and Cultivation Techniques on Productivity of Forage Corn in Central and Southern Area of Korea (중부 및 남부지역에서 재배환경과 재배기술의 차이가 사료용 옥수수의 생산성에 미치는 영향)

  • Choi, Gi Jun;Lee, Ki Won;Choi, Ki Choon;Hwang, Tae Young;Kim, Ji Hye;Kim, Won Ho;Lee, Eun Ja;Sung, Kyung Il;Jung, Jeong Sung
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.39 no.4
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    • pp.195-206
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    • 2019
  • This experiment was carried out to study the effects of different environmental conditions and cultivation techniques on productivity of forage corn in central and southern area of Korea on 2017 and 2018. Average dry matter yield of forage corn at 34 cultivation regions was 13,510kg/ha. Forage productivity of forage corn cultivated at actual production sites have positive correlation with cultivation techniques(p<0.01) but not correlated with cultivation environments. Forage productivity of forage corn have positive correlation with seeding techniques(p<0.01) but not correlated with fertilization techniques. These results suggest that practices of cultivation techniques are more important than cultivation environments for increasing the forage productivity of forage corn. Therefore, yield prediction techniques of forage corn in Korea have to be considered the practices of cultivation techniques along with soil and climate conditions.

Effects of Forage Cutting and Baler Mixing on Chemical Compositions, Fermentation Indices, and Aerobic Stability of Whole Crop Rice Haylage (조사료의 세절과 베일러 내 교반이 총체벼 헤일리지의 영양소 함량, 발효특성 및 호기적 안전성에 미치는 영향)

  • Myeong Ji Seo;Young Ho Joo;Seong Shin Lee;Ji Yoon Kim;Chang Hyun Baeg;Seung Min Jeong;Ki Choon Choi;Sam Churl Kim
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.43 no.1
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    • pp.50-55
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    • 2023
  • The present study investigated the effects of forage cutting and baler mixing on the chemical compositions, fermentation indices, and aerobic stability of whole crop rice (WCR) haylage. The WCR ("Youngwoo") was harvested at 48.4% dry matter and ensiled into a 300 kg bale silo with forage cutting (whole crop without cutting vs. 5 cm of cutting length). The WCR forages were ensiled without baler mixing process (CON) or with (MIX). The concentrations of dry matter, crude protein, ether extract, crude ash, neutral detergent fiber, and acid detergent fiber of whole crop rice before ensiling were 48.4, 9.70, 2.57, 6.11, 41.2, and 23.5%, respectively. The forage cutting did not affect the chemical compositions, fermentation indices, microbes, and aerobic stability of WCR haylage (p>0.05). The CON haylages tend to be higher in NDF content (p<0.10). The MIX haylages had lower in lactate (p=0.019), and lactate:acetate ratio (p<0.001). The MIX haylages had higher in lactic acid bacteria (LAB) (p=0.010). Therefore, this study concluded that the fermentation quality of WCR haylage improved by baler mixing, but had no effects by forage cutting.

A Comparative Study on the Dry Matter Yield and Nutritive value of Festulolium braunii (Festulolium braunii의 건물수량 및 사료가치 비교 연구)

  • ;H. Jacob
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.16 no.3
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    • pp.190-198
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    • 1996
  • This experiment was conducted to evaluate the forage yield and quality potential of Festulolium braunii (Festuca pratensis Huds. $\times$ Lolium multiflotum Lam) as forage source in Korea. The experimental design was a split plot design with four replications from Feb. 1994 to Jan. 1996 at Hohenheim uniwrsity. Main plots were three forage species {Fesruca pratensis(Cosmos), Lolium mult~~orum(Lemtal) and Festulolium braunii(Pau1ita)) and subplots were three N levels (150,300 and 450kgha). No differences in dry matter(DM) yields were noted between Festulolium braunii and Lolium multj7orum, but DM yields for Festulolium braunii was 138 more than for Fesruca prafensis(P<0.01 ). Total DM yields over all species increased linearly with increasing N levels. The peak of DM yields over 2 years mean at 450kglha N levels were higher for Lolium mulriJotum(l5,724kg) than for Festulolium braunii(14,730kg) and for Festuca pratensis(l3,68 l kg) (P<0.01). OMD, ME, NEL and other minerals of Fesrulolium braunii were slightly higher and CP, Ca and Mg slightly lower than thsoe of Fesruca pratensis, but CP, OMD, NEL and minerals were absolutly higher for Fesrulolium braunii than for Lolium mulriflorum(P<0.01). It appears that Festulolium braunii has higher potential for improvement of the forage quality than Fesruca prarensis or Lolium rnulrijlorum. The content of CP, CF and minerals were increased, wherease OMD, ME and NEL were decreased in all species with increasing N levels. Yields of CP, DOM and NEL over 2 yean mean were increased in all species with increasing N levels. CP, DOM and NEL yields of Festulolium braunii were higher than those of Lolium multiflorum or Festuca pratensis(P

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A STUDY ON NUTRITIONAL STATUS OF MACRO MINERALS OF CATTLE IN JAVA IN INDONESIA

  • Kumagai, H.;Ishida, N.;Katsumata, H.;Yano, H.;Kawashima, R.;Jachja, J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.3 no.1
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    • pp.7-13
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    • 1990
  • Nutritional status of cattle in Java in Indonesia was investigated by evaluating P, Ca, Mg, K and Na concentrations in forage and plasma inorganic phosphorus (Pi), Ca and Mg, hematocrit and hemoglobin concentrations of cattle during both rainy and dry seasons in 1988. Investigations were conducted at Jonggol (West Java), Malang (East Java) and Majokerto (East Java). Low P content in forage averaging 0.11% on a dry matter basis, high Ca:P ratio in forage in the rainy season (7.7:1) and low plasma Pi concentrations averaging 4.71 mg/dl were observed in Jonggol. In all three places, Ca content in forages was higher than the requirement according to the NRC standard and plasma Ca concentrations were above the critical level. During the rainy season forty percent of cattle in Malang and Mahokerto showed plasma Mg concentrations below 2mg/dl in spite of normal Mg and K content in forage. Forage samples from each place showed a wide variety in Na content, and Na content of two thirds of forage samples was lower than the requirement according to the NRC standard. Hematocrit and hemoglobin concentrations in Malang and Majokerto showed higher values than the normal range.