• 한국동물위생학회지
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• 제40권3호
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• pp.177-185
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• 2017

In this study, we evaluated the efficacy of a commercial vaccine stress-relieving agent (Anti VS-2 injection) to reduce the adverse effects of foot-and-mouth disease (FMD) vaccination site in 120 pigs on two tested pig farms. The results showed that incidence of pig with abnormal meat, size of abnormal meat and weight of discarded abnormal meat are significantly decreased in Anti VS-2 injection-supplemented FMD vaccinated pig groups (n=60) compared to the non-supplemented FMD vaccinated pig groups (n=60) (P<0.05). And the average economic loss by discard of abnormal meat were estimated 99,382 won or 21,514 won in the non-supplemented FMD vaccinated groups or in the Anti VS-2 injection-supplemented FMD vaccinated groups, respectively, showing that the cost saving effect was 78% (77,869 won). In conclusion, it is expected to reduce the incidence and severity of abnormal meat in the FMD vaccinated pigs and to contribute not only to the reduced economic loss of pig farmers but also to the promotion of food hygiene when the Anti VS-2 injection is mixed with FMD vaccine.

• Journal of Veterinary Science
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• 제24권3호
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• pp.40.1-40.6
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• 2023

Analysis of the VP1 gene sequence of the foot and mouth disease virus (FMDV) is critical to understanding viral evolution and disease epidemiology. A standard set of primers have been used for the detection and sequence analysis of the VP1 gene of FMDV directly from suspected clinical samples with limited success. The study validated VP1-specific degenerate primer-based reverse transcription polymerase chain reaction (RT-PCR) for the qualitative detection and sequencing of serotype O FMDV lineages circulating in India. The novel degenerate primer-based RT-PCR amplifying the VP1 gene can circumvent the genetic heterogeneity observed in viruses after cell culture adaptation and facilitate precise viral gene sequence analysis from clinical samples.

• 한국임상수의학회지
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• 제28권1호
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• pp.113-121
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• 2011

Foot-and-mouth disease (FMD) is a severe vesicular disease of cloven-hoofed animals including domesticated ruminants and pigs. Acute clinical signs may be mild in sheep and goats but are associated with lameness in pigs and mouth lesions with vesicles in cattle. The required condition for a successful pathogen appears to be the ability to counteract both the host innate and adaptive immune response. FMD virus (FMDV) inhibits the induction of antiviral molecules and interferes with the secretory pathway in the infected cell. The surface expression of Major Histocompatibility Complex (MHC) class I molecules is reduced in infected cells. Thus, the ability of the host to recognize and eliminate virus infected cells is decreased. Furthermore, FMDV infection results in a rapid, but transient lymphopenia, reducing the number of T and B cells, and affecting T cell function. The virus appears to premature apoptosis-mediated cell death because it has a very short replication cycle and is able to rapidly produce large amounts of virus. FMDV engages the host protective response at multiple steps to ensure its effective replication and pathogenesis. This review describes the recent pathological and immunological studies to overcome the powerful abilities of FMDV to counteract defense mechanism of host.

• Journal of Microbiology and Biotechnology
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• 제30권1호
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• pp.38-43
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• 2020

Hand, foot, and mouth disease (HFMD) is caused by enterovirus 71 (EV71) in infants and children under six years of age. HFMD is characterized by fever, mouth ulcers, and vesicular rashes on the palms and feet. EV71 also causes severe neurological manifestations, such as brainstem encephalitis and aseptic meningitis. Recently, frequent outbreaks of EV71 have occurred in the Asia-Pacific region, but currently, no effective antiviral drugs have been developed to treat the disease. In this study, we investigated the antiviral effect of salvianolic acid B (SalB) on EV71. SalB is a major component of the Salvia miltiorrhiza root and has been shown to be an effective treatment for subarachnoid hemorrhages and myocardial infarctions. HeLa cells were cultured in 12-well plates and treated with SalB (100 or 10 ㎍/ml) and 10⁶ PFU/ml of EV71. SalB treatment (100 ㎍/ml) significantly decreased the cleavage of the eukaryotic eIF4G1 protein and reduced the expression of the EV71 capsid protein VP1. In addition, SalB treatment showed a dramatic decrease in viral infection, measured by immunofluorescence staining. The Akt signaling pathway, a key component of cell survival and proliferation, was significantly increased in EV71-infected HeLa cells treated with 100 ㎍/ml SalB. RT-PCR results showed that the mRNA for anti-apoptotic protein Bcl-2 and the cell cycle regulator Cyclin-D1 were significantly increased by SalB treatment. These results indicate that SalB activates Akt/PKB signaling and inhibits apoptosis in infected HeLa cells. Taken together, these results suggest that SalB could be used to develop a new therapeutic drug for EV71-induced HFMD.

• Journal of the Korean Data and Information Science Society
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• 제25권2호
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• pp.271-280
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• 2014

구제역은 전염성이 높고 치명적 결과를 유발하는 우제류 전염병이며, 2010/2011년도에 국내에서 발생한 구제역 (2010/2011 구제역)은 사회 및 경제적으로 국가에 재난 수준의 손실을 끼쳤다. 따라서 국가적 차원에서 구제역의 예방과, 발병 시 피해를 줄이려는 많은 노력을 하고 있다. 이러한 노력의 하나로 구제역의 전염 현상을 확률적으로 모형화하고 이해하려는 노력이 필요하다. 영국에서 발생한 2001년 구제역은 그 규모와 피해가 막대하여, 영국에서는 다양한 확률적 모형으로 구제역 전파 현상에 대한 이해를 통하여 미래의 발생에 대비하려는 연구가 이루어져 왔다. 그러나 2010/2011 구제역에 대하여는 확률적 모형을 활용한 연구가 미미한 편이다. 따라서 본 연구에서는 2010/2011 구제역에 대하여 시간-공간 확률 SIR 확률모형을 가정하고 시간과 공간에 따르는 전파 현상에 대하여 고찰한다. 농림수산검역검사본부에서 발표한 구제역 감염데이터와 통계청의 전국농가센서스 자료의 일부인 전체 우제류 농가의 데이터가 본 연구의 분석에 필요한 정도로 상세하지 않으므로 추정 및 보정작업을 통하여 데이터를 보완하였다. 감염데이터를 이용하여 커널함수를 추정하고, 전국 우제류 농장데이터를 이용하여 시뮬레이션을 통하여 모형의 모수를 추정하였다.

• Safe Food
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• 제6권1호
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• pp.16-21
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• 2011

• Safe Food
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• 제6권1호
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• pp.8-10
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• 2011

• Safe Food
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• 제6권1호
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• pp.3-7
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• 2011

• 월간 양돈
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• 제15권9호통권169호
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• pp.140-146
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• 1993

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2005년도 Proceedings of The 2nd Asian Society of Veterinary Pathology Symposium(Vol.2) and 2005 Annual Meeting of The Korean Society of Veterinary Pathology(Vol.9)
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• pp.26-46
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• 2005