• Nutrition Research and Practice
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• 제4권3호
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• pp.229-234
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• 2010

Recently, we found and analyzed vitamin $B_{12}$ in some Korean traditional plant foods which had not reported, yet. This study was to investigate vitamin $B_{12}$ intake and its dietary sources and the vitamin $B_{12}$ status in the very old elderly Koreans. We measured serum vitamin $B_{12}$ level and estimated the amounts of vitamin $B_{12}$ intake from different dietary sources in female elderly Koreans aged 85 and over who had consumed a relatively low animal traditional diet for the whole life. The average age of the subjects (n = 127) was 98.0 years (85-108 years). The assessment on energy and nutrient intake involved a one-day 24-hour recall, and serum vitamin $B_{12}$ concentration was measured by radioimmunoassay. Overall diet pattern was not different between the 85-99 yr-old group and centenarians, except centenarians were taking more dairy product. The average ratio of plant food to animal food consumption was 87.5:12.5 in weight. The average vitamin $B_{12}$ intake of our subjects was 3.2 ${\mu}g$, and 52.7% of subjects consumed under estimated average requirement, 2.0 ${\mu}g$/day. On dietary source, 67.3% of dietary vitamin $B_{12}$ was from meat, eggs and fishes and 30.6% was from plant foods, such as soybean-fermented foods, seaweeds, and kimchi. The average serum vitamin $B_{12}$ concentration was 450.5 pg/mL, and low serum vitamin $B_{12}$ (< 200 pg/mL) was found in 9.6% of subjects. Dietary vitamin $B_{12}$ intake was significantly lower in subjects with low serum vitamin $B_{12}$ (0.79 ${\mu}g$/day) than those with normal serum vitamin $B_{12}$ (3.47 ${\mu}g$/day). There were no significant difference in vitamin $B_{12}$ intake and its dietary sources and serum vitamin $B_{12}$ level between the 85-99 yr- old group and centenarians. In conclusion, several plant-origin foods including seaweed, soybean-fermented foods, and kimchi, may contribute significantly to good vitamin $B_{12}$ status in very old elderly Koreans.

• Journal of Nutrition and Health
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• 제11권4호
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• pp.31-38
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• 1978

Younghung-myun is located on a small island under the jurisdiction of Gyunggi province. The island occupying a total of approximately 40sq km is populated by 5,850 people. Two areas within the island were selected for this survey; 1) Nae 4-ri in Younghung-myun (mainly engaged in farming) 2) Nae 5-ri in Younghung-myun (mainly engaged in fishery) The survey was conducted for the period of 5-12 august 1978. Intake of foods and nutrients of all members in the households surveyed by means of questionnaire was computed based on number of meals a day. On the other hand, daily intake of nutrients by an individual was calculated by sex, age and type of work based on the conversion rate of RDA (Korean Recommended Dietary Allowances for Adult). The results obtained are summarized; 1. Status of food intake Average daily food intake of an individual in the surveyed areas was 1,018gm. Out of 1,018gm above, 490gm which represent 48% of the total intake were from cereals, grains or other vegetative products. Ratio of animal-origin foods was determined as only 16% of the total intake. These findings led us to the conclusion that people in the surveyed areas live heavily on vegetative foods. 2. Status of nutrients intake 1) Overall average intake of calorie by an individual in the surveyed areas was 2026.3Kcal per day. On the other hand, average calorie intake by adult persons was 2,491.3 Kcal per day, which is lower than 2,700 Kcal recommended by RDA by approximately 7.7%. Furthermore, it was observed that most of 2,491.3 Kcal obtained by adult persons was from such vegetative foods as cereals, grains, ect. 2) Overall average intake of protein by an individual in the areas surveyed was 52.2gm per dar. On the other hand, average protein intake among adult persons was 67.7gm a day, which indicates lower intake of protein than 80gm of RDA. It was also found that 84.3% of the protein intake by an adult person was of plant origin. 3) Overall average of fat intake by an individual in the areas surveyed was 18.9gm per day, whereas average fat intake among adult persons was 23. 2gm a day. It was brought to our attention that the average fat intake by adult persons is much lower than 36gm recom mended by RDA. 4) Average calcium intake among adult persons in the areas surveyed was 457.1mg per day, that is somewhat lower than 500mg in RDA. However, average iron intake among adult persons in the areas was slightly higher with 10.5mg a day than 10mg of RDA. 5) With exception with vitamin C, intake of all other vitamin groups, such as vit. A, vit. $B_1$, vit. $B_2$ and niacin, were lower among the inhabitants than recommended by RDA. 3. Economic status Average schooling years of food handlers was 6.5 years with average monthly income of \89,000 in the areas surveyed. Petroleum was used by majority of the households for fuel.

• 한국축산식품학회지
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• 제40권5호
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• pp.746-757
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• 2020

Enterotoxigenic Escherichia coli (ETEC) is the major pathogenic E. coli that causes diarrhea and edema in post-weaning piglets. In this study, we describe the morphology and characteristics of ØCJ19, a bacteriophage that infects ETEC, and performed genetic analysis. Phage ØCJ19 belongs to the family Myoviridae. One-step growth curve showed a latent phase of 5 min and burst size of approximately 20 phage particles/infected cell. Phage infectivity was stable for 2 h between 4℃ and 55℃, and the phage was stable between pH 3 and 11. Genetic analysis revealed that phage ØCJ19 has a total of 49,567 bases and 79 open reading frames (ORFs). The full genomic sequence of phage ØCJ19 showed the most similarity to an Escherichia phage, vB_EcoS_ESCO41. There were no genes encoding lysogeny, toxins, virulence factors, or antibiotic resistance in this phage, suggesting that this phage can be used safely as a biological agent to control ETEC. Comparative genomic analysis in terms of the tail fiber proteins could provide genetic insight into host recognition and the relationship with other coliphages. These results showed the possibility to improve food safety by applying phage ØCJ19 to foods of animal origin contaminated with ETEC and suggests that it could be the basis for establishing a safety management system in the animal husbandry.

• Fisheries and Aquatic Sciences
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• 제26권8호
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• pp.491-499
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• 2023

Lake Tinisu Abaya is home to some fish species. The lake's native fish species include Barbus and Tilapia zilli. Tilapia fish (Oreochromis niloticus L.) were stocked in Lake Tinishu Aabaya in 1997. This study aimed to investigate the feeding behaviors of O. niloticus in Lake Tinishu Abaya to develop an appropriate fisheries management approach in the lake. 428 O. niloticus fish samples (2.5 to 30.9 cm and 1.1 to 475 g) were collected to calculate the diet composition. Out of the total of 428 gut contents examined, 55 (12.85%) were found to be empty and 373 (87.15%) to be containing one or more food items. The diet behavior of the stocked fish in the study lake revealed that phytoplankton (39.5% by volume) and macrophytes (25.81% by volume) were the most noteworthy food items followed by detritus (14.39%) and zooplankton (12.95%). With increasing fish size, the importance of phytoplankton, macrophytes, and detritus increased while the contribution of zooplankton, insects, and other foods with an animal origin decreased. Seasonal variation in the diet composition of fish was evident (t-test; p < 0.05). Macrophytes, zooplankton, and detritus were the dominating food items during the wet season, while phytoplankton predominated during the dry season. This study demonstrated that O. niloticus had phytoplanktivores that primarily consumed phytoplankton and that the seasons and fish size had a significant impact on their feeding preferences. The diet of O. niloticus in Lake Tinishu Abaya comprised foods with both plant and animal origins. It concludes that the dietary habit of O. niloticus in the lake is, generally, the omnivorous type.

• Asian-Australasian Journal of Animal Sciences
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• 제32권6호
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• pp.874-880
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• 2019

Objective: Ozone is considered a strong antimicrobial agent with numerous potential applications in the food industry. However, its high oxidizing potential can induce alterations in foods by acting on the unsaturated fatty acids. The aim of this study was to investigate the effect of ozonation on the oxidative stability of chicken breast meat obtained from animals subjected to different feeding strategies. Methods: Samples were obtained from commercial hybrid chickens (ROSS 508), some of which were nourished with a feed enriched with fats of animal origin, while the lipid source was vegetal for the remaining birds. Samples of meat belonging to both groups were treated with ozone and then analysis was performed to evaluate alterations in physical properties, lipid content, fatty acid profile, and oxidation stability. Results: Ozone induced a significant reduction in drip loss in meat samples obtained from animals nourished with vegetable fats; this nutritional strategy also produced meats leaner and richer in polyunsaturated fatty acids. Thiobarbituric acid reactive substances, useful for the assessment of lipid oxidation, were higher in samples obtained from animals fed with vegetable fats with respect to diet based on the addition of animal fats. Conclusion: The ozone treatment improved the physical parameters of meat samples obtained from animals fed with vegetable fats, however the same samples showed a higher lipid oxidation compared to what observed in the case of the dietary intake of animal fats, probably as a consequence of the marked increase in polyunsaturated fatty acids which are more susceptible to peroxidation.

• Nutritional Sciences
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• 제7권2호
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• pp.113-120
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• 2004

This study was conducted to investigate variables related to dietary fiber intake among sixth grade children in an elementary school in Daejon city. One hundred and forty-seven children completed a questionnaire for determining their socioeconomic background and their food habits. Anthropometric measurements were taken, and a 24-hour diet recall method was used to collect three-day food intakes. The socioeconomic status of the children's families belonged to the upper middle class; 53.0% of their fathers and 25.8% of their mothers had completed college or higher degrees. 27.9% of the mothers had jobs, including part-time jobs. Approximately 30% of the children skipped breakfast, 66.4% of the children preferred animal foods to plant foods, and 52.4% of the children preferred green vegetables to yellow or pale vegetables. Grilled meat dishes, such as Grilled beef rib with seasoning, Bulgogi, grilled pork belly and beef steaks, were the most popular types of food eaten outside home by the children. Daily dietary fiber intake was 14.5 g in boys and 14.5 g in girls, and these intakes are low compared to the standard guidelines. The average intakes of energy and protein of the children were 84.5% and 114.0% of the Korean Recommended Dietary Allowances (RDAs), respectively. Besides energy, riboflavin, iron and calcium intakes were below the RDAs, and especially calcium intake was only 50% of the RDAs. On the other hand, thiamin, niacin, ascorbic acid, protein and phosphorus intakes exceeded the Korean RDAs. Family income or the children's body mass index (BMI) was not directly related to dietary fiber intakes. However, higher dietary fiber intakes tended to be related to higher intakes of green vegetables and fruits. Children with higher dietary fiber intake tended to prefer plant foods to animal foods. Energy and most nutrients, except heme iron and retinol, showed positive relationships with dietary fiber intake. Especially potassium and plant origin protein and calcium were highly correlated with dietary fiber intakes(r＞0.6). From these results, it is concluded that dietary fiber intakes of these sixth grade elementary school children were less than the standard reference and it is anticipated to decrease further in the future with increased incomes. Therefore, increased intakes of dietary fiber by elementary school children should be promoted through nutrition education, together with the development of cooking methods and recipes utilizing green vegetables and fruits.

• 한국축산식품학회지
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• 제25권1호
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• pp.13-19
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• 2005

감마선 조사를 통한 즉석식품 제조용 육가공제품의 미생물학적 및 유전 독성학적 안전성 평가를 실시하였다. 미생물 안전성은 총 4종류의 병원성 미생물(Salmonella typhimurium, Escherichia coli, Staphylococcus aureus 및 Listeria ivanovii)을 이용하여 inoculation test를 실시하였으며 유전 독성학적 평가는 ames test를 통해 조사하였다. 4 종류의 병원성 미생물을 볶은 쇠고기 및 햄에 접종했을 경우 초기 균수는 7 log 정도로 나타났으나 1 kGy로 감마선 조사했을 경우 S. typhimurium은 4 log cycle 정도, E. coli 및 S. aureus는 3 log 및 L. ivanovii는 2 log정도 증식이 억제되었다. 볶은 쇠고기 및 햄에 접종된 4 종류의 병원성 미생물은 3 kGy의 선량으로 감마선 조사했을 경우 검출되지 않았다. 볶은 쇠고기 및 햄에 접종된 4종류의 병원성 미생물의 방사선 조사에 대한 감수성을 측정한 결과 접종된 병원성 미생물의 D/sub 10/ value는 볶은 쇠고기에 접종된 L. ivanovii가 0.48로 가장 감수성이 낮은 것으로 나타났다. 볶은 쇠고기 및 햄을 비조사구와 조사구(10 kGy)로 나누어 S. typhimurium TA98 및 TA100 두 균주를 이용하여 유전 독성학적 안전성을 평가한 결과 대사활성을 시킨 경우와 대사활성을 시키지 않은 경우 모두 추출물의 농도에 따른 복귀돌연변이 집락수의 증가는 확인되지 않아, 즉석 식품제조를 위한 볶은 쇠고기 및 햄의 감마선 조사는 유전독성학적으로 안전한 것으로 나타났다.

• 한국식생활문화학회지
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• 제3권3호
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• pp.245-258
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• 1988

A food life style itself is substrative relations with culture, and is concerned with our daily life. Especially a food taboo comes from the intelligence which resulted from an ancestor's life experience. Accordingly, we can say a food taboo began with human appearance, settled in convention of folklore society, and forms it's own boundary todays. Since a practice of a food taboo is combinded firmly with various sociological factors such as religion, custom, and so on, it is very hard to change, even though the belife of a food taboo is not true. According to the result, first, significant factors effecting on a practice of a food taboo was a level of age, a level of education, religion, family pattern, behavior of subject and her mother for food life management, education of subject and her mother, and religion of subject and her mother. Second, in analysis of factors according to classification of food taboo a practice of a food taboo which classified to food of animal, food of plant and the other food showed significant differences, comparing religion of subject with her mother. Third, a result examined practice frequency and review of science, non science indicated that foods such as Egg of Globefish, Lettuce, Coffee, Persimmon, dried Persimmon, Soybean and Sugar are tabooed on the basis of science, and foods such as Vinegary food, Thieved food, Soup of Tangle and Dogmeat tabooed on the basis of non science. But in the case of Puja, it's basis of science is not identified. These food taboos are tabooed by more then 50% of subjects including who answerd 'there is some case to practice it' Therefore, we should continue analysis of science for the reason of a food taboo, at the same time, provide the origin and try to have a rational food life.

• 한국식생활문화학회지
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• 제36권3호
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• pp.274-283
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• 2021

What is the origin of kimchi and what changes did it go through to become the kimchi of today? Also, what makes kimchi different from other pickled vegetables such as Chinese paochai, Japanese tsukemono, and Western pickles, and sauerkraut, and what is the identity of kimchi? This study is the result of thought on these fundamental questions about kimchi which is the only pickled vegetable distributed around the world that uses a fermented and ripened animal seasoning called jeotgal (salted marine life) to dramatically improve its umami taste and nutritional value, thereby securing its uniqueness. Kimchi has further evolved into a unique and high-quality pickled vegetable through the use of its specialized seasoning, adopting a composite fermentation mechanism and absorbing new ingredients such as red pepper. It is expected that this paper will invigorate the discussion on the firm establishment of the identity of kimchi and the future direction that it may take.

• Bulletin of the Korean Chemical Society
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• 제34권9호
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• pp.2787-2794
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• 2013

Cholesterol and cholesterol oxidation products (COPs) may accumulate in foods of animal origin during processing or storage. An effective and sensitive analytical method was developed by increasing the UV absorption of compounds through derivatization by attaching a chromophore to the functional groups of cholesterols (cholesterol, 20-hydroxycholesterol, 7-ketocholesterol, cholestane-$3{\beta}$-$5{\alpha}$-$6{\beta}$-triol, 25-hydroxycholesterol, and $5,6{\alpha}$-epoxycholesterol). The influences of the reaction time, volume of reaction solvent, amounts of derivatizing reagent, and extraction solvents were investigated, as they may influence the reaction and extraction yield. The derivatized COPs were analyzed simultaneously on a C18 column (2.1 mm i.d. ${\times}$ 100 mm length, $3.5{\mu}m$ particle size) using a gradient elution with water and acetonitrile. The derivatized COPs showed increased sensitivity and selectivity in HPLC/UV-Vis. The LOD and LOQ were in the concentration ranges of 0.018-0.55 mg/kg and 0.059-1.84 mg/kg from the powdered milk. And the accuracy and precision were 78.1-116.7% and 1.1-9.9%, respectively.