• Title/Summary/Keyword: Folin-Dennis's method

Search Result 3, Processing Time 0.016 seconds

Quantitative Analysis of Total Phenolic Content in Apple (사과의 총 페놀함량 정량분석)

  • Whang, Hea-Jeung;Han, Wan-Soo;Yoon, Kwang-Ro
    • Analytical Science and Technology
    • /
    • v.14 no.5
    • /
    • pp.377-383
    • /
    • 2001
  • Total amounts of phenolic compounds of Korean apples were determined by UV-visible spectrphotometry of Folin-Dennis, Prussian-blue and vanillin-HCI, respectively. The analytical data from the method of Folin-Dennis were estimated to be the most reasonable to determine the total amounts of phenolic compounds in Korean apples. It was also confirmed that the amounts of total phenolics could be calculated as chlorogenic acid equivalence, more effectively than that of other phenolic compound equivalence.

  • PDF

Changes of Phenolic Compounds in Korean Apple(Fuji) during maturation (성숙기 사과 중의 페놀계물질 변화)

  • 황혜정
    • The Korean Journal of Food And Nutrition
    • /
    • v.12 no.4
    • /
    • pp.364-369
    • /
    • 1999
  • The changes of phenolics in Korean apple(Fuji) during maturation were analyzed by HPLC and spec-tropotometry. The phenolics were separated through C18 Sep-Pak cartridge in series. Chlorogenic acid caffeic acid p-coumaric acid ferulic acid and ($\pm$)-catethin were indentified by the direct comparison with authentics on HPLC. The total amounts of phenolics were determined by Folin-Dennis's method. The amounts ranged from 70.19mg% to 97.57mg% of wet basis. The concentration of phenolics in apple decreased during the early stage of development and then remained relatively constant during matu-ration .

  • PDF

Chemical Composition of Several Herb Plants (서양 허브식물의 화학성분)

  • Oh, Moon-Hun;Whang, Hea-Jeung
    • Korean Journal of Food Science and Technology
    • /
    • v.35 no.1
    • /
    • pp.1-6
    • /
    • 2003
  • Chemical compositions of several herbs (rosemary, mint, thyme, sage, and lavender) cultivated in Korea were analyzed. Approximate compositions were as follows: moisture $69.92{\sim}82.10%$, crude ash $2.48{\sim}6.15%$, crude fat $0.40{\sim}2.46%$, crude protein $0.84{\sim}1.57%$, and crude fiber $2.48{\sim}6.15%$. Total contents of phenolics determined by Folin-Dennis's method were in the range of $73.24{\sim}197.79mg%$. Contents of minerals, Na, Ca, Mn, P, Mg, Zn, and Fe determined by ICP-AES were $43.0{\sim}112.5,\;177.5{\sim}304.0,\;0.5{\sim}1.5,\;74.0{\sim}218.5,\;57.0{\sim}116.0,\;1.0{\sim}2.0$ and $3.0{\sim}5.0mg%$, respectively. Free sugar contents determined by HPLC were: sucrose $0{\sim}7.61$, glucose $0.94{\sim}15.92$, and rhamnose $0.64{\sim}7.99mg%$. Fatty acids including palmitic, stearic, oleic, linoleic, linolenic, and arachidonic acids were identified by GC. Linoleic and linolenic acid contents were higher than those of palmitic and stearic acids. Aroma components identified by GC-MS were 1,8-cineole, ${\alpha}-phellandrene,\;{\alpha}-terpinene,\;{\beta}-pinene,\;{\beta}-thujone$, borneol, butan-1-ol, cis-sabinene hydrate, ${\delta}-carene,\;{\gamma}-terpinene$, and verbenone.