• Journal of Microbiology and Biotechnology
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• v.19 no.1
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• pp.23-36
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• 2009

In the present work, the metabolic network of primary metabolism of the slow-growing myxobacterium Sorangium cellulosum was reconstructed from the annotated genome sequence of the type strain So ce56. During growth on glucose as the carbon source and asparagine as the nitrogen source, So ce56 showed a very low growth rate of $0.23\;d^{-1}$, equivalent to a doubling time of 3 days. Based on a complete stoichiometric and isotopomer model of the central metabolism, $^{13}C$ metabolic flux analysis was carried out for growth with glucose as carbon and asparagine as nitrogen sources. Normalized to the uptake flux for glucose (100%), cells recruited glycolysis (51%) and the pentose phosphate pathway (48%) as major catabolic pathways. The Entner-Doudoroff pathway and glyoxylate shunt were not active. A high flux through the TCA cycle (118%) enabled a strong formation of ATP, but cells revealed a rather low yield for biomass. Inspection of fluxes linked to energy metabolism revealed that S. cellulosum utilized only 10% of the ATP formed for growth, whereas 90% is required for maintenance. This explains the apparent discrepancy between the relatively low biomass yield and the high flux through the energy-delivering TCA cycle. The total flux of NADPH supply (216%) was higher than the demand for anabolism (156%), indicating additional reactions for balancing of NADPH. The cells further exhibited a highly active metabolic cycle, interconverting $C_3$ and $C_4$ metabolites of glycolysis and the TCA cycle. The present work provides the first insight into fluxes of the primary metabolism of myxobacteria, especially for future investigation on the supply of cofactors, building blocks, and energy in myxobacteria, producing natural compounds of biotechnological interest.

• Nuclear Engineering and Technology
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• v.56 no.5
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• pp.1553-1561
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• 2024

Nondestructive radiography using cosmic ray muons has been used for decades to monitor nuclear reactor and spent nuclear fuel storage. Because nuclear fuel assemblies are highly dense and large, typical radiation probes such as x-rays cannot penetrate these target imaging objects. Although cosmic ray muons are highly penetrative for nuclear fuels as a result of their relatively high energy, the wide application of muon tomography is limited because of naturally low cosmic ray muon flux. This work presents a new image reconstruction algorithm to maximize the utility of cosmic ray muon in tomography applications. Muon momentum information is used to improve imaging resolution, as well as muon scattering angle. In this work, a new convolution was introduced known as M-value, which is a mathematical integration of two measured quantities: scattering angle and momentum. It captures the objects' quantity and density in a way that is easy to use with image reconstruction algorithms. The results demonstrate how to reconstruct images when muon momentum measurements are included in a typical muon scattering tomography algorithm. Using M-value improves muon tomography image resolution by replacing the scattering angle value without increasing computation costs. This new algorithm is projected to be a standard nondestructive radiography technique for spent nuclear fuel and nuclear material management.

• 한국전산유체공학회:학술대회논문집
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• 2003.10a
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• pp.189-190
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• 2003

A New kinetic scheme for the compressible Navier-Stokes equations is developed. While the conventional approach, such as KFVS scheme, employs the splitting algorithm and computes the numerical flux on the basis of the collisionless equation, the present approach employs the splitting algorithm in the evaluation of the numerical flux, where the collision effect is explicitly taken into account. However, the initial condition employed in the computation is slightly different from the conventional Chapman-Enskog NS distribution function. The present study also reveals the background of the existing kinetic schemes. such as the KFVS scheme and Gas-Kinetic BGK scheme.

• Journal of computational fluids engineering
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• v.3 no.2
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• pp.17-26
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• 1998

The three-dimensional incompressible Navier-Stokes equations have been solved by a node-centered finite volume method with unstructured hybrid grids. The pressure-velocity coupling is handled by the artificial compressibility algorithm and convective fluxes are obtained by Roe's flux difference splitting scheme with linear reconstruction of the solutions. Euler implicit method with Jacobi matrix solver is used for the time-integration. The viscous terms are discretised in a manner to handle any kind of grids such as tetragedra, prisms, pyramids, hexahedra, or mixed-element grid. Inviscid bump flow is solved to check the accuracy of high order convective flux discretisation. And viscous flows around a circular cylinder and a sphere are studied to show the efficiency and accuracy of the solver.

• The Bulletin of The Korean Astronomical Society
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• v.41 no.1
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• pp.46.3-47
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• 2016

We construct a solar eruption model with a background solar wind by performing three-dimensional zero-beta magnetohydrodynamic (MHD) simulation. The initial configuration of a magnetic field is given by nonlinear force-free field (NLFFF) reconstruction applied to a flux emergence simulation. The background solar wind is driven by upflows imposed at the top boundary. We analyzed the temporal development of the Lorentz force at the flux tube axis. Based on the results, we demonstrate that a solar eruption is caused by the imbalance between magnetic pressure gradient force and magnetic tension force. We conclude that this imbalance is produced by a weak but continuously existing solar wind above an active region.

• Journal of Biomedical Engineering Research
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• v.30 no.6
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• pp.461-468
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• 2009

Magnetic resonance electrical impedance tomography (MREIT) enables us to perform high-resolution conductivity imaging of an electrically conducting object. Injecting low-frequency current through a pair of surface electrodes, we measure an induced magnetic flux density using an MRI scanner and this requires a sophisticated MR phase imaging method. Applying a conductivity image reconstruction algorithm to measured magnetic flux density data subject to multiple injection currents, we can produce multi-slice cross-sectional conductivity images. When there exists a local region of fat, the well-known chemical shift phenomenon produces misalignments of pixels in MR images. This may result in artifacts in magnetic flux density image and consequently in conductivity image. In this paper, we investigate chemical shift artifact correction in MREIT based on the well-known three-point Dixon technique. The major difference is in the fact that we must focus on the phase image in MREIT. Using three Dixon data sets, we explain how to calculate a magnetic flux density image without chemical shift artifact. We test the correction method through imaging experiments of a cheese phantom and postmortem canine head. Experimental results clearly show that the method effectively eliminates artifacts related with the chemical shift phenomenon in a reconstructed conductivity image.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.24 no.1
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• pp.93-101
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• 2000

The characteristics of spray nozzle have been quantified with the measurement of fluorescence and Mie scattering images. To correct the attenuation of the incident light sheet, a sequential double-pass light sheet system and the geometrical averaging of two images was implemented. Quantitative mass flux distribution of spray was obtained from fluorescence image. 3-D image is reconstructed using 2-D radial images. Sauter mean diameter (SMD) distribution was determined using the ratio of fluorescence signal intensity and Mie scattering signal intensity and the values were quantified with PDP A data. The measurement of mass flux and SMD using planar imaging technique agee with PDP A data fairly well in the low density region. However, in dense region， there are significant errors caused by secondary scattering. It was found that the planar imaging technique provides many advantages over the point measurement technique, such as PDP A, and can be implemented for quantitative measurement, especially in low density region.

• Proceedings of the KSRS Conference
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• 2007.10a
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• pp.33-36
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• 2007

We have persistently constructed gridded products of surface wind/wind stress over the world ocean using satellite scatterometer (ERS and Qscat). They are available for users as the Japanese Ocean Flux data sets with Use of Remote sensing Observation (J-OFURO) data together with heat flux components. Recently, a new version data of the Qscat/SeaWinds based on improved algorithm for rain flag and high wind-speed range have been delivered, and allowed us to reconstruct gridded product with higher spatial resolution. These products are validated by comparisons with in-situ measurement data by mooring buoys such as TAO/TRITON, NDBC and the Kuroshio Extension Observation (KEO) buoys, together with numerical weather prediction model products such as the NCEP-1 and 2. Results reveal that the new product has almost the same magnitude in mean difference as the previous version of Qscat product and much smaller than the NCEP-1 and 2. On the other hand, it is slightly larger root-mean-square (RMS) difference than the previous one and NCEPs for the comparison using the KEO buoy data. This may be due to the deficit of high wind speed data in the buoy measurement. The high resolution product, together with sea surface temperature (SST) one, is used to examine a new type of relationship between the lower atmosphere and upper ocean in the Kuroshio Extension region.

• International Journal of Aeronautical and Space Sciences
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• v.3 no.1
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• pp.74-85
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• 2002

The numerical experiment has been conducted to investigate the unsteady shock wave reflecting phenomena. The cell-vertex finite-volume, Roe's upwind flux difference splitting method with unstructured grid is implemented to solve unsteady Euler equations. The $4^{th}$-order Runge-Kutta method is applied for time integration. A linear reconstruction of the flux vector using the least-square method is applied to obtain the $2^{nd}$-order accuracy for the spatial derivatives. For a better resolution of the shock wave and slipline, the dynamic grid adaptation technique is adopted. The new concept of grid adaptation technique, which is much simpler than that of conventional techniques, is introduced for the current study. Three error indicators (divergence and curl of velocity, and gradient of density) are used for the grid adaptation procedure. Considering the quality of the solution and the numerical efficiency, the grid adaptation procedure was updated up to $2^{nd}$ level at every 20 time steps. For the convenience of comparison with other experimental and analytical results, the case of interaction between the straight incoming shock wave and a sharp wedge is simulated for various flow conditions. The numerical results show good agreement with other experimental and analytical results, in the shock wave reflecting structure, slipline, and the trajectory of the triple points. Some critical cases show disagreement with the analytical results, but these cases also have been proven to show hysteresis phenomena.

• Journal of Electrical Engineering and Technology
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• v.13 no.3
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• pp.1156-1165
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• 2018

This paper presents a detailed realization of direct torque controlled induction motor drive for elevator applications. The drive is controlled according to the well-known space vector modulated direct control scheme (SVM-DTC). As the elevator drives are usually equipped with speed sensors, flux estimation is carried out using a current model where two stator currents are measured and accurate instantaneous rotor speed measurement is used to overcome the need for measuring stator voltages. Speed profiling for a comfortable elevator ride and other supervisory control activities to provide smooth operation are also explained. The drive performance is examined and controllers' parameters are fine-tuned using MATLAB/SIMULINK. The blocks used for flux and torque estimation and control in the offline simulation are compiled for real-time using dSPACE Microlabox. The performance of the drive has been verified experimentally. The results show good performance under transient and steady state conditions.