• Title/Summary/Keyword: Fluorescent image

Search Result 122, Processing Time 0.038 seconds

Application of fluorescent particles as a tracer to detect the membrane surface damage in a pilot scale membrane bioreactor (형광입자를 이용한 분리막 표면 검측 방법의 파일럿 규모 플랜트 적용)

  • Kim, Choah;Kim, Hee Jun;Cho, Jinwoo
    • Journal of Korean Society of Water and Wastewater
    • /
    • v.30 no.1
    • /
    • pp.33-40
    • /
    • 2016
  • In this study, a fluorescent silica nano particle is used as the surrogate for challenging test of membrane surface integrity. The particles are functionalized by a fluorescent dying agent so that as an ultraviolet light is imposed a bright fluorescent image from the particles can be taken. If a membrane surface is damaged and has a compromised part larger than the size of surrogate the fluorescent particles would pass through and contained in the permeate. An operator can directly notice whether the membrane surface is damaged or not by detecting a fluorescent image taken from the permeate. Additionally, the size of compromised part is estimated through analysing the fluorescent image in which we surmise the mass of particles included in the permeate by calculating an average RGB value of the image. The pilot scale experiments showed that this method could be applied successfully to determine if a membrane surface had a damaged parts regardless of the test condition. In the testing on the actual damaged area of $4.712mm^2$, the lowest error of estimating the damaged area was -1.32% with the surrogate concentration of 80 mg/L, flux of $40L/m^2/hr$ for 25 minutes of detection. A further study is still going on to increase the lowest detection limit and thus decrease the error of estimation.

Prefluorescent-Dye-Induced Fluorescent Imaging based on Polymeric Photobase Generators

  • Chae, Kyu-Ho;Choi, Won-San;Kim, Yoo-Ho
    • Proceedings of the Polymer Society of Korea Conference
    • /
    • 2006.10a
    • /
    • pp.177-177
    • /
    • 2006
  • Copolymers containing oxime-urethane groups, which led to the formation of pendant amino groups photochemically, were applied to a fluorescent image-recording material through the reaction with fluorescamine, a prefluorescent dye for amino groups. This imaging method permits the fluorescent image to be erased or restored by treatment with base or acid. Copolymers containing phthalimide carbamate groups were applied to a bicolor fluorescent imaging material through the consecutive reaction with fluorescamine and rhodamine. A various colored fluorescent micropattern (green, red or red-yellow) was observed through the changes of excitation wavelength by using a conforcal microscope.

  • PDF

Fabrication of Microcapsules Encapsulating Fluorescent Nanoparticles and Visualization of Their Inclusion (형광 나노입자를 수용하는 마이크로캡슐의 제작 및 수용 가시화)

  • Kim, Eun-Young;Kim, Hyoung-Hoon;Go, Jeung-Sang
    • Journal of the Korean Society of Visualization
    • /
    • v.9 no.2
    • /
    • pp.16-20
    • /
    • 2011
  • This paper presents a fabrication method of microcapsules encapsulating fluorescent nanoparticles sensitive to an organic liquid, which is potentially applicable to the encapsulation of protein, cell and drug. It uses the supra-molecular self-assembly of a block copolymer at the interface of the stable and controllable droplets of water suspended with fluorescent nanoparticles and the polymer solved organic. The size and uniformity of the microcapsules were examined for the various polymer concentrations by using SEM image analysis. The maximum standard deviation of the produced microcapsules of less than 3.5% was obtained from the microcapsules produced from the same conditions. The inclusion of fluorescent nanoparticles was visualized in the fluorescence microscope and by using TEM image. It is shown that this fabrication method can provide the uniform size microcapsules with a higher inclusion.

Spraying Status Evaluation of the Electro-static Sprayer Using Computer Image Processing (컴퓨터 영상처리를 이용한 정전분무기의 분무상태 평가)

  • Hwang, H.;Cho, S. I.;Cho, D. Y.
    • Journal of Biosystems Engineering
    • /
    • v.24 no.5
    • /
    • pp.391-398
    • /
    • 1999
  • The spraying status of the electrostatic sprayer was evaluated by processing surface spraying images of the natural leaves. Water solution of the fluorescent material was used as a spray medium. The image of the lights reflected by fluorescent droplets was captured under UV light using a color CCD camera. Coverage rate, particle density, and the size distribution of particles were analyzed from the surface images of leaves under various spraying conditions such as spraying nozzle angle and object distance. Spraying characteristics of the electrostatic sprayer was evaluated in comparison with the conventional one. In a case of electrostatic sprayer, coverage rate and particle density increased by the average of 1.57times and 1.01times respectively under various nozzle angles and distances. The number of particle under the diameter of 50 ${\mu}{\textrm}{m}$ also increased significantly.

  • PDF

An image used by cross concept of two images - Gap of image space2 - (두가지 영상의 교차개념을 도입한 영상설치작품 - 상 공간의 틈새2 -)

  • Cho Ok-Hue;Lee Jun-Eui;Kim Hyung-Gi
    • Archives of design research
    • /
    • v.19 no.4 s.66
    • /
    • pp.205-208
    • /
    • 2006
  • This work is a interactive digital art work which used the multiplex of jitter. It is an work which used a cross concept of two images. This work shows two kinds of images. One is on the wall through the gap of fluorescent lamp screen, and the other is on the fluorescent lamp screen. Two images are intercepted and comes out to the length as the multiplex of the jitter. The appearance of the spectator be recognized on the fluorescent lamp screen or on the wall, by two branch image which comes out intersecting of a length with multiplex function of the jitter. Two branch features of the image which it produces and the spectator which is input at real-time is equally divided, or in impression of juxtaposition goes in the spectator. It expresses the duplicity of the human being, with physical existence space divides the time space which we feel and there is a governing grammar which is different with each other and to express the thing it becomes. It used the image of the screen which it produces with the fluorescent lamp and cross concept.

  • PDF

Digital Color Imaging Systems for Quantitative Evaluation of Skin Lesions (피부병변의 정량적 평가를 위한 디지털 컬러 영상 시스템)

  • Han, Byung-Kwan;Jung, Byung-Jo
    • Journal of Biomedical Engineering Research
    • /
    • v.28 no.2
    • /
    • pp.195-198
    • /
    • 2007
  • In this paper, we introduce a digital cross-polarization and fluorescent color imaging system for quantitative evaluation of skin lesions. We describe the characterization of the imaging systems and the quantitative image analysis methods to show the feasibility for quantitative evaluation of skin lesions. The polarization color image was used to compute erythema and melanin index image which are useful for quantitative evaluation of pigmentation and vascular skin lesions, respectively. The fluorescent color image was used to quantitatively evaluate "sebum" and "vitiligo". In quantitative evaluation of various skin lesions, we confirmed the clinical efficacy of the imaging systems for dermatological applications. Finally, we sure that the imaging systems can be utilized as important assistant tools for the evaluation of skin lesions by providing reproducible quantitative result for widely distributed skin lesions.

The effects of short persistent CCFL in Blinking Back Light Unit to reduce blur on TFT-LCD

  • Han, J.M.;Bae, K.W.;Kim, S.Y.;Kim, Y.H.;Lim, Y.J.
    • 한국정보디스플레이학회:학술대회논문집
    • /
    • 2003.07a
    • /
    • pp.694-697
    • /
    • 2003
  • In applying LCD to TV application, one of the most significant factors to be improved is image sticking on the moving picture. LCD is different from CRT in the sense that it's continuous passive device, which holds images in entire frame period, while impulse type device generate image in very short time. To reduce image sticking problem related to hold type display mode, we made an experiment to drive LCD like CRT. We made articulate images by turn on-off backlight, and we realized the ratio of Back Light on-off time by adjusting between on time and off time for video signal input during 1 frame (16.7ms). Conventional CCFL (cold cathode fluorescent lamp) cannot follow fast on-off speed, so we evaluated new fluorescent substances of light source to improve residual light characteristic of CCFL. We realized articulate image generation similar to CRT by blinking drive. As a result, reduced image sticking phenomenon was validated by naked eye and response time measurement.

  • PDF

Algorithm to Shorten Imaging Time in Fluorescent X-ray Computed Tomogrpahy (형광 X선 CT에서 촬상 시간의 단축화 알고리즘)

  • 정남채
    • Journal of the Institute of Convergence Signal Processing
    • /
    • v.2 no.4
    • /
    • pp.46-52
    • /
    • 2001
  • In this paper it was examined both imaging system and processing algorithm for imaging's high speedization of fluorescent X-ray computed tomography using synchrotron radiation, The electronic system was used for dead time by about 6% but shortening of measure time was achieved by 2 seconds per 1 point. Also efficiency of reconstruction algorithm was proved, and memory and calculation amount was decreased by about 1/100 The fixed quantity was confirmed by physical phantom, and iodine distribution was presumed from image of thyroid gland in vitro These result shows realization possibility of fluorescent X-ray computed tomography measure in vivo.

  • PDF

Time-Resolved Two-Phase PIV Measurements of Freely Rising Bubble Flows with an Image Separation Method (단일 카메라의 영상분리를 이용한 자유 상승 기포의 고속 이상 유동 PIV 계측)

  • Sung Jaeyong;Park Sang Min;Yoo Jung Yul
    • Journal of the Korean Society of Visualization
    • /
    • v.2 no.1
    • /
    • pp.39-45
    • /
    • 2004
  • A time-resolved two-phase PIV system using a single camera has been developed, which introduces a method of image separation into respective phase images, and is applied to freely rising single bubble. Gas bubble, tracer particle and background have different gray intensity ranges on the same image frame when reflection and dispersion in the phase interface are intrinsically eliminated by optical filters and fluorescent particles. Further, the signals of the two phases do not interfere with each other. Gas phase velocities are obtained from the separated bubble image by applying the two-frame PTV. On the other hand, liquid phase velocities are obtained from the tracer particle image by applying the cross-correlation algorithm. As a result, the bubble rises rectilinearly just after it is released from an injector and then has a zigzag motion in the far field. From the trajectory of the bubble, it is found that the period of the zigzag motion is closely related to the vortex shedding although the wavelength of it varies along its movement.

  • PDF

Study of Blurring Free TFT-LCD Using Short Persistance Cold Cathode Fluorescent Lamp in Blinking Backlight Driving (단잔광 냉음극관을 이용한 잔상없는 TFT-LCD에 관한 연구)

  • Choi, Dae-Seub;Sin, Ho-Chul
    • Journal of Satellite, Information and Communications
    • /
    • v.7 no.3
    • /
    • pp.145-148
    • /
    • 2012
  • In applying LCD to TV application, one of the most significant factors to be improved is image sticking on the moving picture. LCD is different from CRT in the sense that it's continuous passive device, which holds images in entire frame period, while impulse type device generate image in very short time. To reduce image sticking problem related to hold typedisplay mode, we made an experiment to drive TN-LCD like CRT. We made articulate images by turn on-off backlight, and we realized the ratio of Back Light on-off time by counting between on time and off time for video signal input during 1 frame (16.7ms). Conventional CCFL (cold cathode fluorescent lamp) cannot follow fast on-off speed, so we evaluated new fluorescent substances of light source to improve residual light characteristic of CCFL. We realized articulate image generation similar to CRT by CCFL blinking drive and TN-LCD overdriving. As a result, reduced image sticking phenomenon was validated by naked eye and response time measurement.