• Proceedings of the KSME Conference
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• 2003.11a
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• pp.694-699
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• 2003

Simultaneous measurement with PLIF(Planar Laser-Induced Fluorescence) and Stereo-PIV(Stereoscopic Particle Image Velocimetry) was performed to investigate the structural characteristics of flow field in Rushton Turbine Mixer. Instantaneous 3D velocity fields are measured by two 2K ${\times}$ 2K CCD cameras focused on an object plane with the angular displacement methods while the concentration fields are obtained through the measurement of the fluorescence intensity of Rhodamine B tracer excited by the second pulse of Nd:Yag laser light. Image distortion due to the camera view-angle is compensated by a mapping function. Finally, the spatial structures of turbulent mixing around Rushton turbine were identified by the calculation of cross-correlation fields between the velocity and concentration field.

• 한국가시화정보학회:학술대회논문집
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• 2004.12a
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• pp.91-110
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• 2004

A comprehensive three-dimensional nano-particle tracking technique in micro- and nano-scale spatial resolution using the Total Internal Reflection Fluorescence Microscope (TIRFM) is discussed. Evanescent waves from the total internal reflection of a 488nm argon-ion laser are used to measure the hindered Brownian diffusion within few hundred nanometers of a glass-water interface. 200-nm fluorescence-coated polystyrene spheres are used as tracers to achieve three-dimensional tracking within the near-wall penetration depth. A novel ratiometric imaging technique coupled with a neural network model is used to tag and track the tracer particles. This technique allows for the determination of the relative depth wise locations of the particles. This analysis, to our knowledge is the first such three-dimensional ratiometric nano-particle tracking velocimetry technique to be applied for measuring Brownian diffusion close to the wall.

• Proceedings of the KSME Conference
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• 2000.11b
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• pp.353-358
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• 2000

To achieve the requirement for high fuel economy and low emissions, the research for GDI engines is recently very brisk in the whole world. This study was performed to measure distribution of average particle size in non-evaporating spray. The 2-D fluorescence/scattering images of fuel spray were captured simultaneously by visualization system composed of a laser sheet, a doubling prism, optical filters, and an ICCD camera. Using the ratio of the two light intensities, particle size distribution was obtained. The SMD measured by fluorescence/scattering technique was compared with it obtained by PDA. The experimental results show that the spray structure of GDI injector and temporal SMD distribution.

• Journal of Korean Society for Atmospheric Environment
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• v.12 no.5
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• pp.587-591
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• 1996

An intercomparison experiment was implemented to assess the uncertainties and precisions om atmospheric SO$_{2}$ measurement techiques including a pulsed fluorescence (P-F), a diffusion-scrubber /ion chromatography (D-I), and a mist-chamber/ion chromatography (M-I). Each of those three techniques was investigated by researchers at Seoul National University, Yonsei University, and Hankuk University of Foreign Studies, respectively. The concentrations of atmospheric SO$_{2}$ were determined concurrently using three independent measurement techniques at the Seoul National University campus, Korea during Nov.22 to Dec.2, 1995. While the results from the P-F and D-I techinques showed the very close agreements (within +-5%) throughout the experiment period, M-I technique showed systematically smaller values (up to 30%) than the other two techniques. Although sources of larger discrepancy between different techniques were mot identified, the lower SO$_{2}$ values of the M-I method may be related to the errors associated with sample collection effciency, mass flow rate measurements, and standardization of ion chromatography.

• Journal of the Korean Society of Visualization
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• v.1 no.1
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• pp.98-106
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• 2003

A PLLIF (Planar Liquid Laser Induced Fluorescence) technique has been known to be a useful tool for the measurement of the spray patterns for various spray injectors because it can obtain two-dimensional images with high spatial resolutions without any intrusion on the spray field. In case of dense spray, however, the secondary emission as well as the extinction of an incident laser beam or a fluorescence signal can cause errors in quantifying a mass distribution. Unfortunately, a like-doublet injector which has a dense spray zone at the center may not be a good example or the application of the PLLIF technique. Therefore, we took PLLIF data for the like-doublet injector with a 12 bit color CCD camera by varying laser power, and then assessed their accuracy by comparing with the data obtained with a mechanical patternator and a PDPA (Phase Doppler Particle Analyzer). The experimental results showed that the gray level of fluorescence signal increases nonlinearly due to a secondary emission at the dense spray zone but this nonlinearity can be avoided by reducing the incident laser beam power. In addition, the mass flux distribution of the spray could be obtained by using the mass concentration data from PLLIF technique and the velocity profiles of liquid drops, and this distribution showed good agreement with that of mechanical pattemator. Therefore, it is possible that the PLLIF technique can be successfully applied to finding the mass distributions of like-doublet injectors.

• Journal of the Korean Society of Propulsion Engineers
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• v.4 no.1
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• pp.36-45
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• 2000

Most researches for impinging jet spray have been focused on under-standing the breakup mechanism of a liquid sheet formed by the collision of jets and modeling the spray breakup using experimental data. For this reason, there have been few studies on the characteristics of the spatial spray distribution which affects significantly the combustion efficiency. Hence, we measured the radial distribution of fuel massflux using a like-doublet type injector. Instead of PDPA(Phase Doppler Particle Analyzer) which has been used only for the point measurement of the drop size of spray, PLIF(Planar Laser Induced Fluorescence) technique was developed lot the 2-D measurement of the massflux distribution of spray Indirect photography technique was also used to verify PLIF data.

• Journal of the Korean Society of Visualization
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• v.7 no.1
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• pp.9-13
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• 2009

Fluorescence recovery after photobleaching (FRAP) has been widely used for the measurement of molecular diffusion in living cells and tissues. We developed an image-based FRAP (iFRAP) technique using a modified real-time microscope and a 488 nm Ar-ion laser. A fractional intensity curve was obtained from the time-lapse images of fluorescence recovery in the bleached spot to determine the diffusion coefficient of fluorescently labeled macromolecules in porous medium. We validated iFRAP through experiments with agar gels (0.5% and 1.5% w/v) containing FITC-Dextrans (10, 70 and 500 kDa MW). Further validation was performed by a Monte Carlo approach, where we simulated the three-dimensional random walk of macromolecules in agar gel model. Diffusion coefficients were deduced from the mean square displacement curves and showed good agreements with those measured by iFRAP.

• Proceedings of the KSME Conference
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• 2001.06d
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• pp.25-30
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• 2001

The liquid fuel film on the cylinder liner is believed to be a major source of engine-out hydrocarbon emissions in SI engines, especially during cold start and warm-up period. Quantifying the liquid fuel film on the cylinder liner is essential to understand the engine-out hydrocarbon emissions formation in SI engines. In this research, two-dimensional visualization was carried out to quantify liquid fuel film on the quartz liner in an SI engine test rig. The visualization was based on laser-induced fluorescence and total reflection. Using a quartz liner and a special lens, only the liquid fuel on the liner was visualized. The calibration technique was developed to quantify the fluorescence signal with the thickness gage and the calibration device. The fluorescence intensity increases linearly with increase in the fuel film thickness on the quartz liner. Using this technique, the distribution of the fuel film thickness on the cylinder liner was measured quantitatively for different valve lifts and injected fuel mass in the test rig.

• Korean Journal of Environmental Agriculture
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• v.39 no.2
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• pp.138-141
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• 2020

BACKGROUND: Fresh weight is one of the major quality measurement factors in determining the quality of fresh fruits. A practical method has been developed for rapid and non-destructive measurement using the Chlorophyll Fluorescence Image (CFI) technique to estimate changes in fresh weight of post-harvest products. METHODS AND RESULTS: Kiwifruit (Actinidia deliciosa) was used and measured for the fresh weight and CFI under different temperature conditions at 0, 10, and 20℃, from 0 to 21 days after storage (DAS). We observed the fresh weight of kiwifruit and measured the surface image for determining F_v/F_m value in terms of maximum quantum yield on each day. To estimate freshness of kiwifruit we applied linear regression between the measured fruit weights and F_v/F_m values. Results showed that fruit weights were reduced by 4% at 0℃, 6% at 10℃, and 14% at 20℃ for 21 days, respectively. And also, the value of Fv/Fm was shown as decreasing trend at all temperature conditions, especially at 20 ℃. Fv/Fm values showed highly significant correlation (R²>0.9) with fresh weight of kiwifruit at all different storage temperatures. CONCLUSION: Thus, CFI technique can be useful to estimate the fresh weight of kiwifruit.

• ETRI Journal
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• v.28 no.6
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• pp.770-776
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• 2006

This paper presents a hyperspectral imaging technique based on laser-induced fluorescence for non-invasive detection of tumorous tissue on mouse skin. Hyperspectral imaging sensors collect image data in a number of narrow, adjacent spectral bands. Such high-resolution measurement of spectral information reveals contiguous emission spectra at each image pixel useful for the characterization of constituent materials. The hyperspectral image data used in this study are fluorescence images of mouse skin consisting of 21 spectral bands in the visible spectrum of the wavelengths ranging from 440 nm to 640 nm. Fluorescence signal is measured with the use of laser excitation at 337 nm. An acousto-optic tunable filter (AOTF) is used to capture images at 10 nm intervals. All spectral band images are spatially registered with the reference band image at 490 nm to obtain exact pixel correspondences by compensating the spatial offsets caused by the refraction differences in AOTF at different wavelengths during the image capture procedure. The unique fluorescence spectral signatures demonstrate a good separation to differentiate malignant tumors from normal tissues for rapid detection of skin cancers without biopsy.