• Title/Summary/Keyword: Fluorescence Quenching Method

Search Result 44, Processing Time 0.023 seconds

CdTe Quantum Dots as Fluorescent Probes for Josamycin Determination

  • Peng, Jinyun;Nong, Keliang;Mu, Guangshan;Huang, Fengying
    • Bulletin of the Korean Chemical Society
    • /
    • v.32 no.8
    • /
    • pp.2727-2731
    • /
    • 2011
  • A new method for the determination of josamycin has been developed based on quenching of the fluorescence of 3-mercaptopropionic acid-capped CdTe quantum dots (MPA-CdTe QDs) by josamycin in ethanol. Reaction time, interfering substances on the fluorescence quenching, and mechanism of the interaction of CdTe QDs with josamycin were investigated. Under optimum conditions, the relative fluorescence intensity was linearly proportional to the concentration of josamycin between 12.0 and 120.0 ${\mu}g\;mL^{-1}$ with a correlation coefficient of 0.9956 and a detection limit of 2.5 ${\mu}g\;mL^{-1}$. The proposed method was successfully applied to commercial tablets, and the results were satisfactory, i.e. consistent with those of high-performance liquid chromatography (HPLC).

An Apparatus for Monitoring Real-time Uranium Concentration Using Fluorescence Intensity at Time Zero

  • Lee, Sang-Mock;Shin, Jang-Soo;Kang, Shin-Won
    • Nuclear Engineering and Technology
    • /
    • v.33 no.2
    • /
    • pp.166-174
    • /
    • 2001
  • An apparatus for detecting remote real-time uranium concentration using an optrode was developed. An optrode to detect uranium fluorescence as remote real-time control was designed. Fluorescence intensity at time 2ero was derived by the fluorescence signal processing and the algorithm to exclude the quenching effect of various quenchers and temperature fluctuations. This apparatus employing the above deriving method and the optrode has an error range within 6% in spite of serious fluorescence lifetime changes due to the quenching effect and temperature fluctuations. The detection limit is 0.06 ppm and the linearity is excellent between 0.06 ppm and 2 ppm on the aqueous uranium solution.

  • PDF

Spectroscopic investigations on the interaction of bovine serum albumin with amoxicillin and cloxacillin

  • BHALCHANDRA P. KAMAT,
    • Journal of Photoscience
    • /
    • v.12 no.1
    • /
    • pp.11-15
    • /
    • 2005
  • The mechanism of interaction of two drugs viz., amoxicillin and cloxacillin with bovine serum albumin has been investigated using fluorescence absorption and circular dichroism spectroscopy. The quenching mechanism of fluorescence of bovine serum albumin by amoxicillin and cloxacillin was discussed. The binding sites number n and apparent binding constant Kwere measured by fluorescence quenching method. The thermodynamic parameters obtained from data at different temperatures were calculated. The distance r between donor (bovine serum albumin) and acceptor (amoxicillin and cloxacillin) was obtained according to Forster theory of non-radiative energy transfer. The effect of common ions on binding constant was also investigated. The results of synchronous fluorescence spectra, UV-vis absorption spectra and circular dichroism of BSA in presence of amoxicillin and cloxacillin show that the conformation of bovine serum albumin changed

  • PDF

EFFECT OF TEMPERATURE ON FLUORESCENCE QUENCHING BY STEADY STATE AND TRANSIENT METHODS IN SOME ORGANIC LIQUID SCINTILLATORS

  • Giraddi, T.P.;Kadadevarmath, J.S.;Chikkur, G.C.;Rath, M.C.;Mukherjee, T.
    • Journal of Photoscience
    • /
    • v.4 no.3
    • /
    • pp.97-103
    • /
    • 1997
  • The effect of temperature on the fluorescence quenching of 2-(4-Methoxyphenyl)-5-(1-naphthyl)-1,3,4-oxadiazole (MPNO1), 2-(4-Methoxyphenyl)-5-(2-naphthyl)-1,3,4-oxadiazote(MPNO2), by aniline, and 2-Phenylindole (2-PI) by CCk, in toluene by steady state method and in benzene by time-resolved method have been carried out in the temperature range 30 - 70$\circ$C. The Stem-Volmer (S-V) plots, I$_0$/I against quencher concentration [Q] at different temperanares show positive deviations. The fluorescence lifetimes determined at different temperatures show no systematic variations and the variations being within the experimental error, the average values of lifetimes $ $\tau$ (t) are taken for further calculations. Rate constants such as Stem-Volmer quenching constants K$_sv}$, quenching rate parameters k$_q$ and k'$_q$, static quenching constant V and kinetic distance r are determined using the modified Stem-Volmer equation and sphere of action static quenching model. In order to see whether the reactions are diffusion limited, equations k$_q$ = e$^{-Eq/RT}$ and k'$_q$ = e$^{-Eq/RT}$ are used to determine the values of E$_q$ and E'$_q$, the activation energies for collisional quenching and the values of E$_q$ are 14.53. 17.28 and 16.20 kJ mole$^{-1}$ for MPNO1, MPNO2 and 2-PI respectively and the values of E'$_q$ are 14.62 and 17.73 for MPNO1 and MPNO2 respectively. From the magnitudes of various quantities it has been concluded that the reactions are diffusion limited and the observed positive deviations in the S-V plot are due to static and dynamic quenching.

  • PDF

Fluorescent Magnetic Silica Nanotubes with High Photostability Prepared by the Conventional Reverse Micro-Emulsion Method

  • Zhang, Yuhai;Son, Sang Jun
    • Bulletin of the Korean Chemical Society
    • /
    • v.33 no.12
    • /
    • pp.4165-4168
    • /
    • 2012
  • Magnetic fluorescent silica nanotubes were fabricated using reverse micro-emulsions coupled with conventional sol-gel methods. Anodic aluminum oxide templates were used to separate spatially the magnetic and the fluorescent moieties on individual nanotubes and so prevent quenching of the fluorescence. C18 and fluorescent layers were deposited sequentially on silica. Magnetism was then obtained by the introduction of pre-made magnetic nanoparticles inside the nanotubes. The photo- and chemical stabilities of nanotubes were demonstrated through dye release and photobleaching tests. The produced nanotubes did not show fluorescence quenching upon the addition of the nanoparticles, an advantage over conventional spherical fluorescent magnetic nanoparticles. High photostability of nanotubes, magnetism and biocompatiblily make them potentially useful in bioanalysis.

Comparative and Structural Analysis of the Interaction between β-Lactoglobulin type A and B with a New Anticancer Component (2,2'-Bipyridin n-Hexyl Dithiocarbamato Pd(II) Nitrate)

  • Divsalar, A.;Saboury, A.A.;Mansoori-Torshizi, H.;Hemmatinejad, B.
    • Bulletin of the Korean Chemical Society
    • /
    • v.27 no.11
    • /
    • pp.1801-1808
    • /
    • 2006
  • The interaction between whey carrier protein $\beta$-lactoglobulin type A and B (BLG-A and -B) and 2,2'-bipyridin n-hexyl dithiocarbamato Pd(II) nitrate (BPHDC-Pd(II)), a new heavy metal complex designed for anticancer property, was investigated by fluorescence spectroscopy combined with chemometry and circular dichroism (CD) techniques. A strong fluorescence quenching reaction of BPHDC-Pd(II) to BLG-A and -B was observed. Hence, BPHDC-Pd(II) complex can be bound to both BLG-A and -B, and quench the fluorescence spectra of the proteins. The quenching constant was determined using the modified Stern-Volmer equation. The binding parameters were evaluated by fluorescence quenching method. The results of binding study provided evidences presence of two and three sets of binding sites on the BLG-B and -A, respectively, for BPHDC-Pd(II) complex. Using fluorescence spectroscopy and chemometry, the ability of BLG-A and -B to form an intermediate upon interaction with BPHDC-Pd(II) complex was assessed. CD studies displayed that under influence of different concentrations of BPHDC-Pd(II) complex, the regular secondary structure of BLG-B had no significant changes, whereas for BLG-A a transition from $\alpha$-helix to $\beta$-structure was appeared. The results for both of BLG-A and -B displayed that BPHDC-Pd(II) complex can induce a conformational transition from the native form to an intermediate state with a slightly opened conformation, which is detectable with chemometry analyses.

Fluorimetric Determination of Phosphate in Sea Water by Flow Injection Analysis

  • Motomizu, Shoji;Oshima, Mitsuko;Katsumura, Naoya
    • Analytical Science and Technology
    • /
    • v.8 no.4
    • /
    • pp.843-848
    • /
    • 1995
  • A sensitive method for the determination of trace amounts of phosphate by fluorescence-quenching detection / FIA is proposed. The fluorescence of Rhodamine B(RB) was quenched with the formation of the ion associate of molybdophosphate with RB;${\lambda}_{ex}$ and ${\lambda}_{em}$ were 560nm and 580nm, respectively. A calibration graph was linear over the ranges from $10^{-8}$ to $3{\times}10^{-6}M$ of phosphate (~0.3~93ppb of phosphorus). The relative standard deviation was 1.2% with $8{\times}10^{-7}M$ phosphate solution and sampling rate was 15 samples / h. The proposed method was applied to the determination of phosphate in sea and river water samples.

  • PDF

Conformational and Structural Changes of Choline Oxidase from Alcaligenes Species by Changing pH Values

  • Hekmat, A.;Saboury, A. A.;Divsalar, A.;Khanmohammadi, M.
    • Bulletin of the Korean Chemical Society
    • /
    • v.29 no.8
    • /
    • pp.1510-1518
    • /
    • 2008
  • Results of intrinsic and extrinsic fluorescence studies on choline oxidase revealed that the enzyme at high alkaline pH values has more accessible hydrophobic patches relative to acidic pH. Fluorescence quenching studies with acrylamide confirm these changes. The quenching constants were also determined at different pH(s) by using the Stern-Volmer equation. CD studies showed that at higher pH a transition from $\alpha$-helix to $\beta$- structure was appeared while at lower pH the content of $\alpha$-helix structure was increased. Furthermore, analysis of the spectral data using chemometric method gave evidence for existence of intermediate components at very high pH(s). Results of thermal denaturation evaluated that the enzyme has the most instability at higher pH(s). Altogether low and high pH values caused significant alteration on secondary and tertiary structures of choline oxidase via inducing of an intermediate.

Kinetic Study on the Low-lying Excited States of Ga Atoms in Ar

  • Kuntack Lee;Ju Seon Goo;Ja Kang Ku
    • Bulletin of the Korean Chemical Society
    • /
    • v.15 no.8
    • /
    • pp.663-669
    • /
    • 1994
  • Decay kinetics of Ga(5s), Ga(5p) and Ga(4d) atoms in Ar were studied by laser induced fluorescence technique. Theground state gallium atoms in the gas phase were generated by pulsed dc discharge of trimethyl gallium and argon mixtures. Both pulsed discharge and YAG-DYE laser system were controlled by a dual channel pulse generator and the delay time between the end of discharge and laser pulses was set 3.0-6.0 ms. The Ga(5s) and Ga(4d) atoms were generated by single photon excitation from the ground state Ga atoms and radiative lifetimes as well as the total quenching rate constants in Ar were obtained from the pressure dependence of the fluorescence decay rates. The Ga(5p) atoms were populated by a two-photon excitation method and the cascade fluorescence from Ga(5s) atoms were analyzed to extract quenching rate constant of Ga(5p) atoms by Ar in addition to radiative lifetimes of Ga(5p) state. The magnitudes of the quenching rate constants by Ar for the low-lying excited states of Ga atoms are 1.6-3$ {\times}10^{-11}cm^3$ molecul$e^{-1}s^{-1}$, which are much larger than those for alkali, alkaline earth and Group 12 metals. Based on the measured rate constants, kinetic simulations were done to assign state-to-state rate constants.

Research Method of Fatty Acids Transfer between Phospholipid Model Membranes (인지질 모델막에서의 지방산 이동에 관한 연구 방법)

  • 임병순;김혜경;김을상
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.26 no.4
    • /
    • pp.743-750
    • /
    • 1997
  • Direct measurement of the kinetics of free fatty acid transfer between phospholipid model membrane is technically limited by the rapid nature of the transfer process. Separation of membrane-bound fatty acid by centrifugation has shown that although the equilibrium distribution of free fatty acid is determined by this method, fatty acid transfer occurs too rapidly for accurate kinetic measurements. Recently fluorescence resonance energy transfer(FRET) assay has been developed to examine transfer of fatty acids between membranes. Donor membranes which has fluorescent fatty acid, anthroyloxy fatty acid(AOFA), is mixed with acceptor membranes which has non-interchangeable fluorescent quencher, nitrobenzo-xadiazol(NBD), using stopped flow apparatus. As the fluorescent fatty acids transfer from donor membrane to acceptor membrane, fluorescence intensity would be decreased and the rate and degree of fatty acid transfer can be analyzed. Fatty acid transfer between micelles is more complicated because of bile salt. Therefore in experiments with micelles, fluorescence self quenching assay is used. At high concentrations, a fluorophore tends to quench its own fluorescence causing a reduction in fluorescence intensity. Donor micelles contained self quenching concentrations of fluorophore and acceptor micelles had no fluorophore. Upon mixing of donor and acceptor micelles, the rate of transfer of the fluorophore from the donor to the acceptor was measured by monitoring the release in self quenching when its concentration in donor decreased over time.

  • PDF