• 한국연초학회지
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• 제13권2호
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• pp.20-26
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• 1991

This study was carried out to develop the method of alkaloids analysis and investigate the kinds and contents of alkaloids in flue-cured, burley and oriental tobacco leaves using developed analytical method. The developed analytical method of alkaloids was as followed : Tobacco sample was treated with acid(pH 2∼3) and extracted with chloroform to remove chemical components except alkaloids. Sample solution was treated with alkali(pH 12∼ 13) and was extracted with chloroform to obtain alkaloids from sample solution. After extraction of alkaloids from tobacco leaves, alkaloids were separated and identified by GC, GC/MS using SE-54 fused silica capillary column. Nicotine, nornicotine, myosmine, 9-nicotyline, anabasine, anatabine, 2, 3-bipyridyl, cotinine, formyl nornicotine, acetyl nornicotine and formal anatabine were isolated and identified from the extracts of tobacco leaves. The contents of alkaloids were burley > flue-cured > oriental tobacco leaves, but oriental tobacco leaves were higher nornicotine, cotinine, formyl nornicotine and acetyl nornicotine contents than those of flue-cured tobacco leaves. The burley tobacco harvested in Korea was higher nornicotine contents by 2-6 times in the portions of cutter, leaf and tips position than that of burley tobacco(B3F) harvested in V. S. A.

• 한국연초학회지
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• 제9권2호
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• pp.49-57
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• 1987

This study had been conducted to determine morphological characteristics of soil in the major districts cultivating flue-cured tobacco plant. Also native soil productivities were measured by means of bioassay planting tobacco plant without fertilizer at 87 selected soils through field experiment. Morphological characteristics of troll affecting the dry weight of tobacco leaves cultivated in the field were investigated Among soil morphological characteristics, topograpy, slope, topsoil depth, soil depth, and soil texture had influenced with high significant on the dry weight of tobacco leaves. For prediction of dry weight (productivity) of tobacco leaves without fertilizer , multiple regression analysis were introduced using soul morphological characteristics. A combination of topography, slope, topsoil depth, and soil texture was very reliable for prediction of productivity. The regression equation was y = -16.88 -14, 34$X_1$ +20.43$X_2$ +50.21$X_3$ -7.54$X_4$ +13.45$X_5$ R = $0.670^{**}$ Where $X_1$ : Topography $X_2$ : Slope $X_3$ : Topsoil Depth $X_4$ : Soil Depth $X_5$ : Soil Texture

• 한국연초학회지
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• 제2권1호
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• pp.68-77
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• 1980

土壤中 有效燐酸 含量이 相異한 圃地(350, 140, 35ppm)에 燐酸質 肥料를 0, 5, 10, 15, 20, 30kg/10a로 各各 處理하였을 때 담배의 生育과 品質에 미치는 효과를 調査하였다. 담배의 生育은 生育後期에 下位地(35ppm)만 燐酸質 肥料 增施 效果가 認定되었으며 乾物重은 담배 生育과 同一한 傾向이었다. 담배의 成熟과 葉中 化學成分은 燐酸質 肥料에 影響을 받지 않았다. 乾葉의 品質은 土壤中 有效燐酸 含量에 關係없이 비슷하였으며 收量은 下位地에서만 燐酸質 肥料의 增施效果가 認定되어 10kg/10a 施肥면 충분한 生産量을 期待할 수 있는 것으로 나타났다.

• 한국연초학회지
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• 제21권1호
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• pp.89-94
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• 1999

This study was carried out to survey the distribution of farm-yard manure by analysis 355 samples collected from major flue-cured and burley tobacco area. The applied manure amount for flue-cured and burley tobacco were 1,500 kg and 1,600 - 2,000 kg per l0a, respectively. The major raw materials of farm-yard manure were cattle dung, chaff, and rice straw. The main manure used for tobacco plant were cattle dung+chaff, cattle dung+rice straw+chaff, cattle dung+rice straw, and poultry dung+chaff, which were mostly unfinished fermentation materials. The chemical characteristics of farm-yard manure were as follows: pH for 87% of samples was higher than 7.1, total nitrogen for 70% of samples was 0.51-1.50%, P$_2$O$_{5}$ for 48% of samples was higher than 1 %, $K_2$O and CaO were 1-2%, MgO was 0.5-1.0%, and Cl was lower than 0.5% level. In general, the manure used for tobacco cultivation was higher contents of chemical components than that of manure made by rice straw. Also, the concentration of chemical compounds in the samples collected from burley tobacco area were higher than that of flue-cured tobacco districts.s.

• 한국연초학회지
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• 제26권1호
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• pp.17-26
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• 2004

This study was conducted to get the informations for reducing the variation of chemical contents of leaf tobacco. The contents and variations of some chemical constituents of leaf(Leaf, Grade 2) produced in various growing areas from 1999 to 2003 and the effects of meteorological factors on the chemical constituents of leaf were analysed. The contents of analysed constituents of leaf showed high significant differences among crop years in flue-cured and burley, particularly the variation among crop years were higher in chlorine and nicotine contents while lower in total nitrogen content. There were significant differences among growing areas in nicotine and total sugar contents of flue-cured leaf and chlorine content of burley leaf. The total sugar content were negatively correlated to the nicotine and total nitrogen contents in flue-cured leaf. The average air temperature in June and July were positively correlated to the nicotine content of leaf while negatively to total sugar, and the precipitation in May were negatively correlated to the nicotine while positively to total sugar.

• 한국연초학회지
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• 제22권2호
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• pp.143-150
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• 2000

This study was carried out to investigate the effect of storage period on the physico-chemical properties of tobacco leaves expanded by $CO_2$. The flue-cured and burley tobacco leaves produced from 1996 to 1999 were processed, and stored for that year or 3 years in processing plant. As compared with 1996 crop, the expanded rate of flue-cured tobacco leaves in 1999 crop was higher approximately 18%, while that of burley was higher about 10%. The filling capacity of cigarettes was decreased as storage time became shorter, but the combustibility was improved. The rate of large particles (over 3.36mm) of expanded tobacco showed decreasing tendency as the storage period became longer. The change rate of chemical contents in tobacco leaves between before and after expansion had no significant difference among corp years. The tar and CO contents of cigarettes smoke were reduced in the case of flue-cured tobacco leaves as storage time became shorter, while they were slightly increased in the case of burley tobacco leaves. The ammonia content of burley leaves was decreased remarkably in 1996 and 1999 crop.

• 한국연초학회지
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• 제21권2호
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• pp.128-135
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• 1999

This study was conducted to determine the sidestream smoke concentration, nicotine, tar, and CO, by the characteristics of tobacco leaves and their combinations. 20 kinds of tobacco leaves and 15 types of their combinations were selected for this study. After collecting the sidestream smoke by fishtail chimney, the concentrations of nicotine, tar, and CO in sidestream smoke were analyzed. Variation in nicotine concentration of sidestream smoke among tobacco leaves and their combinations was as much as 9-times. Heavy leaves and burley leaves were higher in nicotine concentration than light leaves and flue-cured leaves, respectively, the reconstituted tobacco leaf had the minimum concentration of nicotine. Tar concentration of sidestream smoke also was changed by the characteristics of tobacco leaves and their combinations. As the american C4F had the maximum concentration of tar in sidestream smoke, the reconstituted tobacco leaf showed the minimum concentration. Blending 50 % flue-cured B1-0 plus 50% burley B1-T grade showed the highest value in the nicotine concentration. Also, in case of adding B3K and izmir tobacco leaves, the nicotine concentration was decreased. The tar concentration in the sidestream smoke increased as increasing the amount of flue-cured heavy leaf. The CO concentration was not so much Changed by the characteristics of tobacco leaves and their combinations.

• 한국연초학회지
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• 제27권2호
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• pp.207-211
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• 2005

This study was carried out to develop calibration equation of total volatile bases of tobacco leaf using near infrared spectroscopy(NIRS). Burley, imported flue-cured and oriental leaf tobacco samples were collected in 2005 crop year. Calibration equation was developed by modified partial least square method. The standard error of calibration and $R^2$ between traditional analytical method and NIRS analytical method were $0.038\%$, 0.983 for burley and $0.027\%$, 0.986 for imported flue-cured and oriental leaf, respectively. The standard error of performance and $R^2$ between traditional analytical method and NIRS analytical method were $0.048\%$, 0.940 for burley and $0.024\%$, 0.986 for imported flue-cured and oriental leaf, respectively. From these results, the NIRS analytical method seems to be applicable in analyzing total volatile bases of tobacco.

• 한국연초학회지
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• 제24권1호
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• pp.13-20
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• 2002

This study was conducted to evaluate the prediction of sensory attributes of tobacco smoke by the use of volatile oil components of tobacco. For analytical and sensory evaluations, twelve aging tobaccos (i.e., 3 crop years; 1998, 1999, 2000, and 4 stalk positions) were prepared in flue-cured and burley tobaccos. 61 volatile oil components and 5 sensory attributes such as irritation, impact, after taste, bitter and green were investigated. Irritation of flue-cured tobacco, and irritation and impact of burley tobacco were significantly increased with the ascending stalk position, whereas after taste of burley tobacco was significantly decreased. Significantly positive correlations among irritation, impact, and bitter were observed in flue-cured tobacco. A significantly positive correlation between irritation and bitter was observed, significantly negative correlations between after taste and irritation and between after taste and impact were observed in burley tobacco. Except for green of burley tobacco, all probabilities of multiple linear regression equations between volatile oil components of tobacco and sensory attributes of tobacco smoke were significant(P$\leq$0.05). This study suggests that the multiple linear regression equations may be useful to predict the sensory attributes of tobacco smoke with a few selected volatile oil components of tobacco.

• 한국연초학회지
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• 제23권1호
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• pp.45-52
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• 2001

This study was carried out to evaluate the uncertainty in the analysis of total alkaloids and reducing sugar content in flue-cured tobacco. The sources of uncertainty associated with the analysis of total alkaloids and reducing sugar were the weighing of sample, the preparation of extracting solution, the addition of extracting solution into the sample, the preparation of standard solution, the precision of calibration curve for standard solution, the reproducibility of analysis, and the determination of water content in tobacco, etc. For the calculating uncertainties, Type A of uncertainty was evaluated by the statistical analysis of a series of observation, and Type B by the information based on supplier’s catalogue and/or certificated of calibration. It was shown that the main source of uncertainty was caused by the calibration curve of standard solution, the reproducibility of analysis, the volume measurement of 1$m\ell$, and the purity of nicotine reference material in the preparation of standard solution. The uncertainty in the addition of extracting solution, the sample weighing, the volume measurement of 100$m\ell$, and the determination of water content of tobacco contributed relatively little to the overall uncertainty. The expanded uncertainty of total alkaloids and reducing sugar in flue-cured tobacco at 95% level of confidence was $\pm$0.12% and $\pm$0.54%, respectively.