• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• v.34 no.4
• /
• pp.412-418
• /
• 2008

Bone morphogenetic proteins (BMPs) in combination with stem cells gain more significance for their use in bone tissue engineering. The mesenchymal stem cell can be differentiated into osteoblast by the treatment of BMP. The aim of this study is to characterize the osteogenic differentiation process of adult stem cells derived from buccal fat pad according to BMP-2 within culture media and decide the appropriate concentration of BMP-2 to facilitate osteogenesis. The authors procured the stem cell from buccal fat pad and analyzed for presence of stem cell by flow cytomety against CD-34, CD-105 and STRO-1. The buccal fat derived stem cells (BFDC) were treated by application of the different concentration with BMP-2 of 0, 10, 50, 100 and 200ng/ml, respectively. And their ability to differentiate into osteogenic pathway were checked by alkaline phosphatase(ALP) staining, Alizarin red staining and RT-PCR for osteocalcin(OC) gene expression at 7, 14 and 21day of culture. Flow cytometric analysis and biochemical assays demonstrated that BFDC might be a distinguished stem cells, and mineralization was accompanied in proportion to BMP-2 concentration. However, with 100ng/ml concentration of BMP-2, the BFDC demonstrated most efficient staining pattern of ALP and Alizarin red. The feasibility of the osteogenic differentiation in the group of both 50ng/ml and 200ng/ml of BMP-2 showed similar activity and relatively weaker than that of 100ng/ml. These results suggest that the BMP-2 stimulate osteogenesis by BFDC effectively and that bone induction might be controlled through negative regulatory feedback in higher concentration.

• The Journal of Korean Medicine
• /
• v.20 no.2
• /
• pp.88-97
• /
• 1999

To characterize the effects of Gilgyung-Tang(GGT) on cellular proliferation and viability of normal lung fibroblast cells, we examined the cell cycle progression and cell cycle-related gene expression in T3891 using a flow cytometry and a quantitative RT-PCR analysis. 1. The significant surpression effect of cellular proliferations of GGT was observed in proportion to a certain concentration and time. 2. GGT was identified to induce apoptotic death of damaged cells by treatment with a DNA-damage agent and etoposide, while it stimulated the recovery of cellular viability of normal cells. 3 The significant reductions of mRNA expression of PCAN, c-Fos treated by GGT were observed. 4. The significant inductions of mRNA expression of p53, CDKN1. Gadd45 treated by GGT were observed. 5. The apoptosis caused by the reduction of Bcl-2 genes was significant and the Bax genes were increased. but the amount of Fas genes were not changed. These results strongly suggest that GGT triggers arrest of the cell cycle at G1 phase, and thus causes an inhibition of cellular proliferation of human normal lung cells through the transcriptional up-regulation of cell cycle inhibitory genes and down-regulation of induction of cell cycle stimulating genes respectably.

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.5
• /
• pp.2313-2318
• /
• 2014

Peroxisome proliferator-activated receptor gamma (PPAR-${\gamma}$), a ligand-dependent nuclear transcription factor, has been found to widely exist in tumor tissues and plays an important role in affecting tumor cell growth. In this study, we investigated the effect of PPAR-${\gamma}$ on aspects of the cervical cancer malignant phenotype, such as cell proliferation and apoptosis. Cell growth assay, Western blotting, Annexin V and flow cytometry analysis consistently showed that treatment with troglitazone (TGZ, a PPAR-${\gamma}$ agonist) led to dose-dependent inhibition of cervical cancer cell growth through apoptosis, whereas T0070907 (another PPAR-${\gamma}$ antagonist) had no effect on Hela cell proliferation and apoptosis. Furthermore, we also detected the protein expression of p53, p21 and Mdm2 to explain the underlying mechanism of PPAR-${\gamma}$ on cellular apoptosis. Our work, finally, demonstrated the existence of the TGZ-PPAR-${\gamma}$-p53 signaling pathway to be a critical regulator of cell apoptosis. These results suggested that PPAR-${\gamma}$ may be a potential therapeutic target for cervical cancer.

• The KSFM Journal of Fluid Machinery
• /
• v.11 no.2
• /
• pp.29-37
• /
• 2008

Air supply is required to the cathode of fuel cells for the provision of oxygen to produce electricity through chemical reaction with hydrogen in the cell, and supplied air should be free of impurities such as oil mist and tiny particles separated from sliding surfaces. Hence, air compressor for fuel cell air supply must be oil-less type and have no severe sliding surfaces inside. This paper introduces the concept of single-vane type rotary air compressor whose structure is particularly suitable for the fuel cell application: sliding action of the vane against the cylinder wall, which causes severe friction in the conventional vane rotary compressors, is made to be prevented by attaching the vane to the driving shaft with the compliant device between the vane and the rotor in this new design. For 2 kW fuel cell application, preliminary design has been carried out, and its performance has been estimated by using computer simulation program: for discharge pressure of 2 bar, the volumetric, adiabatic, and mechanical efficiencies are calculated to be 82.5%, 92.5%, and 96.3%, respectively.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
• /
• v.24 no.7
• /
• pp.9-19
• /
• 2010

Fuel cell (FC) distributed generation (DG) is gradually becoming more attractive to mainstream electricity users as capacity improves and costs decrease. New technologies including inverters are becoming available to provide a uniform standard interconnection of DGs with an electric power system. Some of the operating conflicts and the effect of DG on power quality are addressed and investigated through simulations on a real distribution network with an FC power plant. The results of these simulations have proved load tracking capability following the real and reactive power change of the load and have shown the flow of overcurrent from an FC power plant during the ground fault of a distribution line.

• International Journal of Oral Biology
• /
• v.32 no.3
• /
• pp.113-118
• /
• 2007

Treatment of oral cancers with chemotherapeutic agents become evaluated as an effective method to reduce cancer cell proliferation. Anti-proliferative and anti-oral cancer activities of momordin I on oral cancer cells were evaluated in this study. Momordin I was originally purified from a natural product, Ampelopsis radix and showed the antiproliferative activity against oral carcinoma, KB cells. Obtained $IC_{50}$ value was approximately $10.4{\mu}g/ml$. Time-and dose-dependent chromosomal DNA fragmentations were observed in momordin I-treated KB cells. Flow cytometry analysis showed time-dependent apoptotic cell appearance after treatment of momordin I. Approximately 18.6% apoptotic cells were observed at 72 hours after $20{\mu}g/ml$ of momordin I treatment. These observation were consistent with the results obtained in DNA fragmentation analysis. These data suggest that momordin I has anti-proliferative effect and induces cell death in KB cells through apoptosis.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2004.10a
• /
• pp.397-400
• /
• 2004

The direct extrusion with porthole die can produce condenser tube which has the competitive power in costs and qualities compared with the existing conform extrusion. In general, porthole die extrusion has a great advantage in the forming that produces the hollow sections difficult to produce by conventional extrusion with a mandrel on the stem. Especially, condenser tube manufactured by porthole die belongs to sophisticated part and demands tighter dimension tolerance and higher surface finish than any other part. In order to confirm the general of porthole die extrusion, we perform the 3D FE analysis of hot porthole extrusion in non-steady state by using DEFORM 3D and investigate a pattern of elastic deformation for porthole die through the stress analysis using ANSYS 5.5 during extrusion process.

• Proceedings of the KIEE Conference
• /
• 1994.07a
• /
• pp.85-87
• /
• 1994

Feeding biological cells one by one is the key point in the manipulation of cells. The conventional valve systems have many difficulties in feeding cells one by one, because they shut the whole flow of fluids when they are closed and have possibilities of breaking the fragile cells. They need some other equipments for continuous supply of suspension and to protect the cells. We design a check valve for feeding biological cells one by one using polyimide all the silicon substrate. The cells are fed by hydraulic pressure through the isotropically etched cavity. When the suspension flows continuously along the channel the valve is bent by hydraulic pressure and a cell is fed to the outlet. We have studied a cell fusion device fabricated with polyimide and electroplating. If the designed check valve is located in front of the cell fusion device it is helpful to fuse two different kinds of cells.

• Journal of the Korean Society for Precision Engineering
• /
• v.25 no.1
• /
• pp.115-121
• /
• 2008

In this paper, a three-dimensional computational fluid dynamic model of a proton exchange membrane fuel cell(PEMFC) with serpentine flow channel is presented. A steady state, single phase and isothermal numerical model has been established to investigate the influence of the GDL (Gas Diffusion Layer) parameters. The GDL is made of a porous material such as carbon cloth, carbon paper or metal wire mesh. For the simplicity, the GDL is modeled as a block of material having numerous pathways through which gaseous reactants and liquid water can pass. The porosity, permeability and thickness of the GDL, which are employed in the model parameters significantly affect the PEMFC performance at the high current region.

• Journal of Power Electronics
• /
• v.19 no.5
• /
• pp.1278-1288
• /
• 2019

A DC-DC Non-Isolated Three-Port Cuk-Cuk (NI-TPCC) converter for interfacing renewable energy sources (RESs) such as Fuel Cell (FC) and Photovoltaic (PV) energy with a DC load is presented in this paper. It features single-stage power conversion from both of the input ports to the load port. The proposed NI-TPCC converter is designed based on the classical Cuk converter. The operational modes and power flow are analyzed in the continuous conduction mode (CCM), and the relationships among the port voltages are derived. Continuous currents in all three ports with less ripple enhance the performance of a fuel cell and its operating life. Furthermore, the output inductor is shared with both of the input ports, which reduces the number of active and passive components. The effectiveness of the designed NI-TPCC converter has been validated through simulation and experimental results.