• Journal of Yeungnam Medical Science
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• 제35권1호
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• pp.84-88
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• 2018

A 33-year-old woman visited the emergency department presenting with fever and dyspnea. She was pregnant with gestational age of 31 weeks and 6 days. She had dysuria for 7 days, and fever and dyspnea for 1 day. The vital signs were as follows: blood pressure 110/70 mmHg, heart rate 118 beats/minute, respiratory rate 28/minute, body temperature $38.7^{\circ}C$, and oxygen saturation by pulse oximetry 84% during inhalation of 5 liters of oxygen by nasal prongs. Crackles were heard over both lung fields. There were no signs of uterine contractions. Chest X-ray and chest computed tomography scan showed multiple consolidations and air bronchograms in both lungs. According to urinalysis, there was pyuria and microscopic hematuria. She was diagnosed with community-acquired pneumonia and urinary tract infection (UTI) that progressed to severe sepsis and acute respiratory failure. We found extended-spectrum beta-lactamase producing Escherichia coli in the blood culture and methicillin-resistant Staphylococcus aureus in the sputum culture. The patient was transferred to the intensive care unit with administration of antibiotics and supplementation of high-flow oxygen. On hospital day 2, hypoxemia was aggravated. She underwent endotracheal intubation and mechanical ventilation. After 3 hours, fetal distress was suspected. Under 100% fraction of inspired oxygen, her oxygen partial pressure was 87 mmHg in the arterial blood. She developed acute kidney injury and thrombocytopenia. We diagnosed her with multi-organ failure due to severe sepsis. After an emergent cesarean section, pneumonia, UTI, and other organ failures gradually recovered. The patient and baby were discharged soon thereafter.

• Journal of Korean Neurosurgical Society
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• 제62권2호
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• pp.153-165
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• 2019

Objective : Spinal cord injury (SCI) is a very serious health problem, usually caused by a trauma and accompanied by elevated levels of inflammation indicators. Stem cell-based therapy is promising some valuable strategies for its functional recovery. Nestin-positive progenitor and/or stem cells (SC) isolated from pancreatic islets (PI) show mesenchymal stem cell (MSC) characteristics. For this reason, we aimed to analyze the effects of rat pancreatic islet derived stem cell (rPI-SC) delivery on functional recovery, as well as the levels of inflammation factors following SCI. Methods : rPI-SCs were isolated, cultured and their MSC characteristics were determined through flow cytometry and immunofluorescence analysis. The experimental rat population was divided into three groups : 1) laminectomy & trauma, 2) laminectomy & trauma & phosphate-buffered saline (PBS), and 3) laminectomy+trauma+SCs. Green fluorescent protein (GFP) labelled rPI-SCs were transplanted into the injured rat spinal cord. Their motilities were evaluated with Basso, Beattie and Bresnahan (BBB) Score. After 4-weeks, spinal cord sections were analyzed for GFP labeled SCs and stained for vimentin, $S100{\beta}$, brain derived neurotrophic factor (BDNF), 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNPase), vascular endothelial growth factor (VEGF) and proinflammatory (interleukin [IL]-6, transforming growth factor $[TGF]-{\beta}$, macrophage inflammatory protein [MIP]-2, myeloperoxidase [MPO]) and anti-inflammatory (IL-1 receptor antagonis) factors. Results : rPI-SCs were revealed to display MSC characteristics and express neural and glial cell markers including BDNF, glial fibrillary acidic protein (GFAP), fibronectin, microtubule associated protein-2a,b (MAP2a,b), ${\beta}3$-tubulin and nestin as well as anti-inflammatory prostaglandin E2 receptor, EP3. The BBB scores showed significant motor recovery in group 3. GFP-labelled cells were localized on the injury site. In addition, decreased proinflammatory factor levels and increased intensity of anti-inflammatory factors were determined. Conclusion : Transplantation of PI-SCs might be an effective strategy to improve functional recovery following spinal cord trauma.

• 복식문화연구
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• 제27권3호
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• pp.222-238
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• 2019

This study is aimed at applying the philosophical concept of "$diff{\acute{e}}rance$" to contemporary men's fashion design, which could effectively show the shift in thinking. For the study method, the author examined the theoretical background of deconstruction and prepared the standard of analysis by organizing the concept of "$diff{\acute{e}}rance$," which exists at the center of philosophy. This study selected the three most popular brands: Maison Margiela, Comme des Garcons, and Yohji Yamamoto. The results are as follows. First, there is expression of perpetual reservation emphasizing time. This includes texture, patchworks, hems with ladder, and exposure of seam that expresses the trace of time flow and delayed delivery of immediate meaning, and the expression creates delay between major and minor and leads to consistent role exchange. Second, the trace of blank that emphasizes the space gives a visual sense of weight on spares with the intentionally granted space, as well as the space that is the trace of trace and creates a sense of depth through the direction of empty space. Third, the space created in the process of wearing cloth is visualized, the structure of clothing is deconstructed, and the movement from the process recomposes the shape of space and expands the definition that enables expansion of time and space. Fourth, the undetermined relationship expresses the border of time and space visually and deconstructs time and space. The approaches are mostly constructive, demonstrating an avant-garde form of clothes-wearing to show the non-form or imbalance condition.

• 한국시뮬레이션학회논문지
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• 제28권1호
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• pp.1-9
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• 2019

최근 물류창고의 중요성이 높아지고 있는 가운데 소량 다빈도 및 빠른 납품에 대응하기 위해 피킹 프로세스 개선에 많은 노력을 기울이고 있다. 본 연구는 개별오더 피킹 정책이 사용되는 상황에서 오더를 피커에게 할당하는 알고리즘을 제시한다. 이 알고리즘에서는 피킹과 관련된 피킹 처리시간, 오더 접수후 경과 시간, 검수/패킹 작업대 상황, 피커 오류, 고객 중요도의 다섯 개의 속성을 동시에 고려한다. 각 속성에 대한 측정값의 단위가 동일하도록 긴급도라는 척도를 도입한다. 긴급도의 값이 클수록 할당 우선순위를 높게 설정한다. 제안한 알고리즘에서는 각 속성의 가중치를 변경함으로써 피킹시스템의 운영목표에 따라 할당 정책을 유연하게 조정할 수 있도록 하였다. 가상의 축소된 물류창고를 대상으로 시뮬레이션 실험을 수행한 결과 시스템 쓰루풋과 흐름시간 측면에서 우수한 성능을 보였다.

• 한국전자통신학회논문지
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• 제15권6호
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• pp.1045-1054
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• 2020

본 논문에서는 불안정한 네트워크 환경에서 안정적으로 대용량 데이터 스트림을 전송할 수 있는 TCP/IP 기반 링 버퍼 시스템을 설계하였다. 우리가 제안한 방식에서 각 전파천문대의 백엔드 시스템으로부터 UDP 프레임으로 생성 및 출력되는 관측 데이터 스트림은 클라이언트 시스템 내의 소켓 버퍼를 경유해 대용량 링 버퍼에 UDP 패킷으로 저장된다. 이후 목적지에 해당하는 상관센터로의 안정적인 전송을 위해 해당 패킷은 TCP로 전송되고 상관센터 내의 서버 시스템은 소켓 버퍼에 수신된 패킷에 이상이 없으면 대용량 링 버퍼에 저장한다. 패킷 손실, 중복 및 순서 역전 등의 이상이 발생할 경우 TCP의 흐름 제어를 통해 패킷은 재전송되며 상관센터에 도착한 데이터는 신뢰성을 보장받게 된다. 또한 네트워크 성능 불안정으로 인한 혼잡 회피 발생 시 병렬 스트림 적용을 통해 성능 저하가 최소화될 수 있도록 하였다.

• 의료ㆍ복지 건축 : 한국의료복지건축학회 논문집
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• 제27권4호
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• pp.7-14
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• 2021

Purpose: This study summarized architectural planning data for clinical practice spaces for CPX/OSCE and Simulation spaces, through analysis of domestic and foreign literature and a survey of domestic colleges of medicine. Methods: This study analyzed the architectural planning elements of the clinical practice spaces of the college of medicine, through a literature review on the clinical practice space and the survey of four recently established colleges of medicine. Results: ① It is desirable to plan the floor plan type for Simulation and CPX/OSCE spaces as a corridor-separated type that separates the circulations between students and staff (faculty). ② The simulation area arranges their practice rooms around the control space where the circulation of the staff is made. Conversely, it is desirable to arrange the CPX/OSCE rooms around the test waiting room where students enter. ③ It is desirable to separate access to the staff and students to allow student control, and to install various support spaces facing the staff corridor to secure the movement of equipment and the flow of evaluators. ④ In the simulation area, the equipment stored in the storage must be set in each room before the practice according to the setting of delivery or surgery like, and the equipment that has been removed must be back, so the circulation is short and does not intersect with the student circulations. ⑤ Debriefing rooms, seminar rooms, etc. are arranged in the staff area for review and discussion of clinical situations where practice and evaluation have been completed. Implications: It is important to configure an appropriate movement system and spatial zoning for each space for simulation and CPX/OSCE.

• Journal of Korea Trade
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• 제23권7호
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• pp.96-112
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• 2019

Purpose - "Trade Facilitation" aims the easier flow of trade across borders, driven not only by effective customs administration, the efficiency of appropriate authorities, but also by telecommunications, the quality of infrastructures and competent logistics. Facilitating trade will help lower trade development costs as well as improve economic development and enhance economic benefits for emerging economies at a time when imports and exports are sent in and out across borders several times in the form of intermediate and final products. Not only that, globalization is being accelerated, which in turn increases competitiveness and this makes logistics one of the key factors when it comes to international trade. Highly efficient logistics services promote product movement, ensure product safety and delivery speed, and reduce trade costs between countries. The purpose of this study is, by using the LPI indices based on gravity model estimates, to analyze the impact of each LPI component on trade with the 20 biggest exporting countries of Northeast Asian countries-Korea, Japan, and China-which account for 19.05% of global exports. Design/methodology - Also, this study statistically analyzes the impact of trade on Northeast Asian countries' top 20 exporting countries, using the LPI indices relevant to Trade Facilitation based on the gravity model estimates. Findings - As a result, it was turned out that the distance, GDP, and the LPI components have relevant impact on the trade exports of all three countries but demonstrated little relation to the demographic perspective. Originality/value - The study also found we can increase the trade volume by improving three countries' trade partners' LPI indices since Korea, Japan, and China share most of their 20 biggest trade partners.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제25권3호
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• pp.194-210
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• 2022

Human milk contains a number of nutritional and bioactive molecules including microorganisms that constitute the so-called "Human Milk Microbiota (HMM)". Recent studies have shown that not only bacterial but also viral, fungal, and archaeal components are present in the HMM. Previous research has established, a "core" microbiome, consisting of Firmicutes (i.e., Streptococcus, Staphylococcus), Proteobacteria (i.e., Serratia, Pseudomonas, Ralstonia, Sphingomonas, Bradyrhizobium), and Actinobacteria (i.e., Propionibacterium, Corynebacterium). This review aims to summarize the main characteristics of HMM and the role it plays in shaping a child's health. We reviewed the most recent literature on the topic (2019-2021), using the PubMed database. The main sources of HMM origin were identified as the retrograde flow and the entero-mammary pathway. Several factors can influence its composition, such as maternal body mass index and diet, use of antibiotics, time and type of delivery, and mode of breastfeeding. The COVID-19 pandemic, by altering the mother-infant dyad and modifying many of our previous habits, has emerged as a new risk factor for the modification of HMM. HMM is an important contributor to gastrointestinal colonization in children and therefore, it is fundamental to avoid any form of perturbation in the HMM that can alter the microbial equilibrium, especially in the first 100 days of life. Microbial dysbiosis can be a trigger point for the development of necrotizing enterocolitis, especially in preterm infants, and for onset of chronic diseases, such as asthma and obesity, later in life.

• Journal of Microbiology and Biotechnology
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• 제34권3호
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• pp.735-745
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• 2024

Avian influenza is a serious threat to both public health and the poultry industry worldwide. This respiratory virus can be combated by eliciting robust immune responses at the site of infection through mucosal immunization. Recombinant probiotics, specifically lactic acid bacteria, are safe and effective carriers for mucosal vaccines. In this study, we engineered recombinant fusion protein by fusing the hemagglutinin 1 (HA1) subunit of the A/Aquatic bird/Korea/W81/2005 (H5N2) with the Bacillus subtilis poly γ-glutamic acid synthetase A (pgsA) at the surface of Lactobacillus casei (pgsA-HA1/L. casei). Using subcellular fractionation and flow cytometry we confirmed the surface localization of this fusion protein. Mucosal administration of pgsA-HA1/L. casei in mice resulted in significant levels of HA1-specific serum IgG, mucosal IgA and neutralizing antibodies against the H5N2 virus. Additionally, pgsA-HA1/L. casei-induced systemic and local cell-mediated immune responses specific to HA1, as evidenced by an increased number of IFN-γ and IL-4 secreting cells in the spleens and higher levels of IL-4 in the local lymphocyte supernatants. Finally, mice inoculated with pgsA-HA1/L. casei were protected against a 10LD50 dose of the homologous mouse-adapted H5N2 virus. These results suggest that mucosal immunization with L. casei displaying HA1 on its surface could be a potential strategy for developing a mucosal vaccine against other H5 subtype viruses.

• Tuberculosis and Respiratory Diseases
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• 제56권5호
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• pp.450-464
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• 2004

연구배경 : FHIT 유전자의 homozygous deletion과 이와 관련된 mRNA 발현 이상, 단백질의 발현 결손은 폐암에서 매우 높은 빈도로 관찰되고 있다. 일부 연구에 의하면 FHIT 유전자를 폐암 세포 내에 이입시켰을 때 apoptosis가 유발되었고, 세포 주기의 이상 소견이 관찰되었으며, 종양형성 능력이 억제됨이 관찰되었다. 하지만 아직까지 FHIT 단백질의 기능에 대한 지식은 미진한 상태이다. 본 연구에서는 FHIT 유전자를 폐암 세포에 이입시켰을 때 유발되는 apoptosis의 기전을 규명하고자 하였다. 방 법 : FHIT 유전자가 결손된 NCI-H358 세포주에 FHIT 유전자를 stable transfection 시킨 후, cisplatin 혹은 paclitaxel을 가하고 apoptosis가 항진되어 나타나는가를 DAPI staining과 flow cytometry로 관찰해 보았다. 또한 이 과정에서 나타나는 caspase system의 변화와 Bcl-2 family의 변화를 Western blotting으로 조사해 보았다. 결 과 : FHIT를 발현시킨 세포에서는 cisplatin 혹은 paclitaxel을 투여하였을 때 유의하게 생존율이 감소하였으며, 이는 apoptosis 증가에 의한 것으로 확인 되었다. 이 과정에서 FHIT가 발현된 세포는 caspase-3, caspase-7의 활성화가 유의하게 증가되었으며, Bcl-2와 Bcl-xL 발현은 유의하게 감소하고 Bax와 Bad 발현은 유의하게 증가하였다. 결 론 : FHIT가 발현된 폐암 세포에 항암제를 투여하였을 때 유의하게 증가한 apoptosis는 caspase system과 Bcl-2 family의 활성화와 관련되어 있다.