• Journal of Microbiology and Biotechnology
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• v.25 no.3
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• pp.413-417
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• 2015

Recently, we isolated HY253, a novel decahydrofluorene analog with a molecular structure of 7,8a-divinyl-2,4a,4b,5,6,7,8,8a,9,9a-decahydro-1H-fluorene-2,4a,4b,9a-tetraol from the roots of Aralia continentalis, which is known as Dokwhal (獨活), a traditional medicinal herb. Moreover, we previously reported its cytotoxic activity on cancer cell proliferation in human lung cancer A549 and cervical cancer HeLa cells. The current study aimed to evaluate its detailed molecular mechanisms in cell cycle arrest and apoptotic induction in human hepatocellular carcinoma HepG2 cells. Flow cytometric analysis of HepG2 cells treated with $60{\mu}M$ HY253 revealed appreciable cell cycle arrest at the G1 phase via inhibition of Rb phosphorylation and down-regulation of cyclin D1. Furthermore, using western blots, we found that up-regulation of cyclin-dependent kinase inhibitors, such as p21^CIP1 and p27^KIP1, was associated with this G₁ phase arrest. Moreover, TUNEL assay and immunoblottings revealed apoptotic induction in HepG2 cells treated with $60{\mu}M$ HY253 for 24 h, which is associated with cytochrome c release from mitochondria, via down-regulation of anti-apoptotic Bcl-2 protein, which in turn resulted in activation of caspase-9 and -3, and proteolytic cleavage of poly(ADP-ribose) polymerase (PARP). Accordingly, we suggest that HY253 may be a potent chemotherapeutic hit compound for treating human liver cancer cells via up-regulation and activation of the p53 gene.

• Journal of Life Science
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• v.19 no.7
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• pp.871-877
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• 2009

We investigated the effects of sodium butyrate, a histone deacetylase inhibitor, on the cell cycle progression in human monocytic leukemia U937 cells. Exposure of U937 cells to sodium butyrate resulted in growth inhibition, G1 arrest of the cell cycle and induction of apoptosis in a dose-dependent manner as measured by MTT assay and flow cytometry analysis. The increase in G1 arrest was associated with the down-regulation in cyclin D1, E, A, cyclin-dependent kinase (Cdk) 4 and 6 expression, and up-regulation of Cdk inhibitors such as p21 and p27. Sodium butyrate treatment also inhibited the phosphorylation of retinoblastoma protein (pRB) and p130, however, the levels of transcription factors E2F-1 and E2F-4 were not markedly modulated. Furthermore, the down-regulation of phosphorylation of pRB and p130 by this compound was associated with enhanced binding of pRB and E2F-1, as well as p130 and E2F-4, respectively. Overall, the present results demonstrate a combined mechanism involving the inhibition of pRBjp130 phosphorylation and induction of Cdk inhibitors as targets for sodium butyrate that may explain some of its anti-cancer effects in U937 cells.

• The Journal of Korean Physical Therapy
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• v.14 no.2
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• pp.32-50
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• 2002

The 30 male and female patients of S-oriental medicine hospital which is located in Taegu, who don't have any caidiovascular diseases and skin diseases, agreed to the experiment for finding out the effects of hot and cold therapy. They were divided into 10 experimental groups, that is hot therapy group(experimental 1 group : 10 People), contrast therapy group(experimental 2 group : 10 People) and mustard hot therapy group (experimental 3 group : 10 People).

• Proceedings of the KIPE Conference
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• 2016.11a
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• pp.29-30
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• 2016

This paper describes a distributed static series compensator (DSSC) which is a type of distributed flexible ac transmission system (DFACTS). The control principles including the power flow control and the current regulation are explained in detail. In order to verify the effectiveness of the DSSC, the simulation results are offered.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.299.1-299.1
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• 2002

Failure of the hepatic microcirculation is a major component of reperfusion injury in the liver. However. the vasoactive mediators involved in the regulation of sinusoidal flow during reperfusion following hepatic ischemia remain to be identified. We investigate the role of Kupffer cells in hepatic ischemia/reperfusion (l/R)-induced imbalance of vasoregulatory gene expression. Rats were subjected to 60 min hepatic ischemia, followed by 5 h of reperfusion. (omitted)

• Biomedical Science Letters
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• v.11 no.3
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• pp.365-373
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• 2005

The aim of this study was to investigate the alterations in meningeal blood flow by stimulation of trigeminovascular system. An open cranial window was prepared on the right parietal bone of male Sprague-Dawley rats. Trigeminovascular system was stimulated by electrical stimulation of trigeminal ganglion (ETS), somatosensory (whisker) stimulation, or topical applications of capsaicin and neuropeptides including substance P and calcitonin gene-related peptide (CGRP). Neonatal capsaicin pretreatment was performed with subcutaneous administration of capsaicin (50 mg/kg) within the first 24 hours after birth. Changes in regional blood flow of dural artery (rDBF) and pial artery (rPBF) were continuously measured through the cranial window by laser-Doppler flowmetry. Both ETS and capsaicin caused a chain of alterations in rPBF and rDBF responses, i.e., an immediate transient decrease followed by rapid and marked increase in rPBF, which were significantly attenuated not only by pretreatments with L-733,060, a $NK_1$ receptor blocker, $CGRP_{8-37}$, a $CGRP_1$ receptor blocker, and 7-nitroindazole monosodium salt (7-NINA), a neuronal nitric oxide synthase inhibitor but also by neonatal capsaicin treatment. Exogenous neuropeptides including substance P and CGRP increased the meningeal blood flow, which was significantly attenuated not only by pretreatment with L-733,060 and $CGRP_{8-37}$, respectively, but also by pretreatment with 7-NINA. The rPBF response to whisker stimulation was significantly attenuated not only by trigeminovascular system injuries including nasociliary nerve denervation and neonatal capsaicin treatment but also by pretreatments with L-733,060, $CGRP_{8-37}$ and 7-NINA. These results suggest that the stimulation of trigeminovascular system causes prominent alterations in meningeal blood flow, and that neuropeptides as well as nitric oxide in the trigeminovascular system are importantly implicated in the regulation of meningeal blood flow.

• The Transactions of the Korean Institute of Electrical Engineers
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• v.39 no.12
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• pp.1269-1280
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• 1990

Owing to the need to accommodate the load variation, thermal power plants need a modern controller in order to achieve better frequency regulation, faster response and higher efficiency. For a successful development of the digital instrumentation and control system that can adopt advanced control algorithms, power plant simulator must be available for safe and convenient test. In this paper, a simple drum-type boiler model is proposed using Signal Flow Diagram that describes the system by a combination of basic time responses representing the relationships between cause and effect process variables. After verifying the model and thereafter building a simulator based on the proposed model, we tested the operation of the controller and stability or efficiency of the implemented control algorithms.

• Proceedings of the Korea Concrete Institute Conference
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• 2004.05a
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• pp.388-391
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• 2004

This research investigates how the fineness modulus of fine aggregates and the grain shape of coarse aggregates affects flow characteristics, packing characteristics and compressive strength characteristic. The experimental results, show that increase of the fine aggregate's fineness modulus improved concrete flow, but filling ability was high at over KS regulation extent due to segregation phenomena. It is considered that the improvement of 0.1 spherical rate was effective to concrete fluidity elevation by reducing about $6\%$ of fine aggregate ratio displays which the smallest gap rate of aggregate. Compressive strength was increased to about 0.6MPa everytime F.M. 0.1 of fine aggregate fineness is increased. However, it was decreased to about 9MPa at F.M. 3.5 compared to F.M. 3.0.

• 제어로봇시스템학회:학술대회논문집
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• 1990.10a
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• pp.194-199
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• 1990

Because of being operated to accomodate the load variation, fossile power plants need modern controller to satisfy frequency regulation, fast response and efficiency requirement. But in the way of developing the Digital Instrumentation and Control System that can adopt advanced control algorithms, power plant simulator must be available for safe and convenient test. In this paper, a simple drum-type boiler model is proposed, using Signal Flow Diagram that describes the system by a collection of basic time response representing the relationship between process variables. After verifying the model and thereafter building simulator based on the proposed model, we test the operation of controller and approve stability or efficiency of implemented control algorithms.

• Proceedings of the KIEE Conference
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• 2008.07a
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• pp.177-178
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• 2008

Recently, the concern of PV(Photovoltaic) systems has increased because of an environmental problem and limitation of fossil fuel. In this paper, distribution system of existing one-way power flow and distribution system generating bidirectional power flow analyze voltage variation at distribution system interconnected multiple PV systems. Also, we analyze a supply voltage range of distribution system using LDC and ULTC voltage regulation method by case study.