• FOCUS: LIFE SCIENCE
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• no.1
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• pp.1.1-1.4
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• 2024

This article provides comprehensive guidance on the maintenance, cleaning, regeneration, and storage of silica-based HPLC (High-Performance Liquid Chromatography) columns. The general considerations emphasize the importance of using in-line filters and guard cartridges to protect columns from blockage and irreversible sample adsorption. While these measures help, contamination by strongly adsorbed sample components can still occur over time, leading to an increase in back pressure, loss of efficiency, and other issues. To maximize column lifetime, especially with UHPLC (Ultra-High Performance Liquid Chromatography) columns, it is advisable to use ultra-pure solvents, freshly prepared aqueous mobile phases, and to filter all samples, standards, and mobile phases. Additionally, an in-line filter system and sample clean-up on dirty samples are recommended. However, in cases of irreversible compound adsorption or column voiding, regeneration may not be possible. The document also provides specific recommendations for column cleaning procedures, including the flushing procedures for various types of columns such as reversed phase, unbonded silica, bonded normal phase, anion exchange, cation exchange, and size exclusion columns for proteins. The flushing procedures involve using specific solvents in a series to clean and regenerate the columns. It is emphasized that the flow rate during flushing should not exceed the specified limit for the particular column, and the last solvent used should be compatible with the mobile phase. Furthermore, the article outlines the storage conditions for silica based HPLC columns, highlighting the impact of storage conditions on the column's lifetime. It is recommended to flush all buffers, salts, and ion-pairing reagents from the column before storage. The storage solvent should ideally match the one used in the initial column test chromatogram provided by the manufacturer, and column end plugs should be fitted to prevent solvent evaporation and drying out of the packing bed.

• Journal of Korea Water Resources Association
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• v.42 no.4
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• pp.319-329
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• 2009

Sediment release openings or pipes are installed in the flood control dam constructed to reduce flood damages, which are to allow water and sediments pass through the dam and to prevent flow blockage and sedimentation in the upstream area of the dam. The Hantan River Flood Control Dam (HRFCD) has been projected for flood damage reduction and sediment release openings and ecological passages are considered for the dam design. In this study, sediment deposition due to the construction of HRFCD was analyzed using the HEC-6 model and compared with the state before the dam construction with respect to the conditions of the annual mean daily discharge and annual discharge hydrograph. According to the numerical results, although downstream water levels were changed by the dam structure, the effects of bed changes were not propagated from the dam over 2 km upstream. Also, 2D numerical models of RMA2 and SED2D were used to predict bed changes in the upstream area with and without sediment release openings. Consequently, it is presented that sediment release openings decreased maximum deposition height in the upstream channel of the dam.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.46 no.3
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• pp.197-209
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• 2018

In this study, numerical analyses on Quad Tilt Rotor(QTR) are carried out to investigate the interference effect of components and effect of operating condition during forward flight. Actuator Surface Method(ASM) which is implemented in an open source CFD code, OpenFOAM, is used to calculate the flow field around QTR with high computational efficiency. The lift of the front and rear wing is found to increase or decrease depending on the rotation direction of the rotor. At the rear wing, the interference effects of the front and rear rotor appear as a combined manner. Performance change due to the phase difference is found to be insignificant. For both rotors, the locally higher thrust is generated by the blockage effect of the wing. The interference effect of wake from the front nacelle contributes to higher local thrust for the rear rotor compared to the front rotor. And it is observed that the amplitude of thrust oscillation can decrease depending on the phase difference between the rotors. Aerodynamic performances of both rotors and the entire aircraft were compared and analyzed for various operating conditions.