• 대한기계학회논문집B
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• 제27권3호
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• pp.275-285
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• 2003

A visualization study on the effect of forcing amplitude in tone-excited jet diffusion flames has been conducted. Visualization techniques are employed using optical schemes. which are a light scattering photography. Flame stability curve is attained according to Reynolds number and forcing amplitude at a fuel tube resonant frequency. Flame behavior is globally grouped into two from attached flame to blown-out flame according to forcing amplitude: one sticks the tradition flame behavior which has been observed in general jet diffusion flames and the other shows a variety of flame modes such as the flame of a feeble forcing amplitude where traditionally well-organized vortex motion evolves, a fat flame. an elongated flame. and an in-burning flame. Particular attention is focused on an elongation flame. which is associated with a turnabout phenomenon of vortex motion and on a reversal of the direction of vortex roll-up. It is found that the flame length with forcing amplitude is the direct outcome of the evolution process of the formed inner flow structure. Especially the negative part of the acoustic cycle under the influence of a strong negative pressure gradient causes the shapes of the fuel stem and fuel branch part and even the direction of vortex roll-up to dramatically change.

• Molecules and Cells
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• 제38권5호
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• pp.416-425
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• 2015

NF-E2-related factor 2 (Nrf2), a basic leucine zipper transcription factor, has recently received a great deal of attention as an important molecule that enhances antioxidative defenses and induces resistance to chemotherapy or radiotherapy. In this study, we investigated the apoptosis-inducing and Nrf2- upregulating effects of quercetin on malignant mesothelioma (MM) MSTO-211H and H2452 cells. Quercetin treatment inhibited cell growth and led to upregulation of Nrf2 at both the mRNA and protein levels without altering the ubiquitination and extending the half-life of the Nrf2 protein. Following treatment with quercetin, analyses of the nuclear level of Nrf2, Nrf2 antioxidant response element-binding assay, Nrf2 promoter-luc assay, and RT-PCR toward the Nrf2-regulated gene, heme oxygenase-1, demonstrated that the induced Nrf2 is transcriptionally active. Knockdown of Nrf2 expression with siRNA enhanced cytotoxicity due to the induction of apoptosis, as evidenced by an increase in the level of proapoptotic Bax, a decrease in the level of antiapoptotic Bcl-2 with enhanced cleavage of caspase-3 and PARP proteins, the appearance of a sub-$G_0/G_1$ peak in the flow cytometric assay, and increased percentage of apoptotic propensities in the annexin V binding assay. Effective reversal of apoptosis was observed following pretreatment with the pan-caspase inhibitor Z-VAD. Moreover, Nrf2 knockdown exhibited increased sensitivity to the anticancer drug, cisplatin, presumably by potentiating the oxidative stress induced by cisplatin. Collectively, our data demonstrate the importance of Nrf2 in cytoprotection, survival, and drug resistance with implications for the potential significance of targeting Nrf2 as a promising strategy for overcoming resistance to chemotherapeutics in MM.

• Journal of Chest Surgery
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• 제19권3호
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• pp.443-448
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• 1986

Aortic dissection is a serious disease that mortality does not approach to zero despite of medical and surgical improvement. Recently two cases of aortic dissection were treated with good results by the two other methods. Case 1 [57-Y-0-Male]; Chief complaint was chest pain radiating to the back. Preoperatively he was controlled by Minipress, dichlotride, & sodium nitroprusside. Aortography showed DeBakey Type III aortic dissection extending from just below the Lt. subclavian artery to the proximal portion of the origin of the renal artery. Through the midline long incision Flow reversal & Thrombo-exclusion method was used, and bypass course was proximal anastomosis at the ascending aorta - through the Rt. thoracic cavity - midportion of the diaphragm - posterior to the liver, stomach, & pancreas - distal anastomosis at the abdominal aorta proximal to its bifurcation. Bypass graft was preclotted 20 mm Dacron Woven Graft, and the aortic arch between the Lt. subclavian artery & Lt. common carotid artery was divided and meticulously sutured. Control aortogram which was done at 4th postoperative month revealed obstruction of the false lumen by thrombosis, and complications were not noticed. Case 2 [53-Y-0-Male]; Chief complaint was chest pain radiating to the abdomen. DeBakey Type III aortic dissection which was similar to the case 1 was detected by the aortography, and involvement of the Lt. subclavian & common carotid arteries was suspicious. Through the Lt. posterolateral thoracotomy the Ringed Intraluminal Sutureless Graft, No. 22 mm, was inserted from just below the Lt. common carotid artery to the midportion of the descending thoracic aorta under total circulation arrest using a F-F bypass, and the Lt. subclavian artery was ligated. Postoperatively hospital course was uneventful with antihypertensive drugs, and any specific complications were not noticed.

• 한국농림기상학회지
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• 제16권3호
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• pp.246-260
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• 2014

90m 고해상도 SRTM 지형자료를 장착한 WRF 수치모형을 이용하여 광릉 활엽수림 KoFlux 타워(GDK)와 침엽수림 KoFlux 타워(GCK)가 위치한 계곡 및 주변에서의 국지 대기순환을 식생-대기 이산화탄소 순환 및 미기상학적 관점에서 연구하였다. 지표 부근 온도와 바람에 대하여 모형과 관측 결과를 비교함으로써 모형의 전반적인 성능을 평가하였고, 연구 지역에 국지순환이 발생한 맑은 날(Case I)과 흐린 날(Case II) 사례를 선정하여 수치모의를 수행하고 그 결과를 정성적으로 분석하였다. 관측된 바람장은 GDK와 GCK 간에, 또 Case I과 Case II 간에 주간 및 야간 국지풍의 시작, 종료, 지속시간, 강도 등에 차이를 보였다. 모형의 수치모의 결과들은 광릉 KoFlux 타워 관측으로부터 그 가능성이 제기된 관측지 계곡의 배수류가 실제로 존재함을 증거하였다. 전반적으로 모형이 모의한 바람장은 아침의 배수류-사면활승풍 전이와 저녁의 사면활승풍 역전 등을 포함하여 계곡 및 주변에서 관측된 국지풍의 일변화를 현실적으로 모사하였다. 또한 국지풍 간 상호작용의 복잡성도 지시하였는데, 가령 주간에 광릉 관측지 계곡 내부의 서풍은 반드시 산풍인 것은 아니었으며, 종종 약한 종관규모 바람 또는 서쪽에서 불어온 해풍과 같은 보다 큰 규모의 중규모 바람 등 다른 풍계와 결합이 되어 있었다. 광릉 생태계의 에너지, 물질, 정보 흐름의 시공간적 변동을 입체적으로 이해하는데 있어서 이러한 고해상도 국지순환 수치모의 결과는 상당히 유용한 것으로 판단되며, 구축된 국지모의 시스템을 지속적으로 개선하는 노력이 향후 뒤따라야 할 것이다. 아울러, 다른 농림생태계 관측지도 모의 영역에 포함시켜 모형 결과의 활용도를 넓힐 필요가 있다.

• 한국해양학회지:바다
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• 제12권3호
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• pp.219-224
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• 2007

광양만의 순환을 이해하기 위하여 2006년 봄철에 수심이 가장 깊은 두 지점을 선정하여 ADCP가 장착된 모니터링 부표 2대를 이용하여 유속관측을 하였다. 두 지점에서 공통적으로 북향류가 지배적인데, 이는 서쪽의 남향류를 동반하는 반시계 방향의 순환 이 있음을 뒷받침 하고 있다. 남쪽에서는 14 m 수심에서 유향이 반전되는 2층 구조가 뚜렷하며 저층의 북향류는 바닥에 가까운 36 m 수심까지 증가한다. 북쪽 지점에서는 북향류가 더욱 강해져서 북쪽의 단면적 감소에 따른 해류의 가속 효과가 있으며 상층부에서도 북향류가 우세하다. EOF 분석을 통한 연직구조에 있어서 첫 번째 모드는 북쪽 부표에서 전체 분산의 74%인데 비하여 남쪽 부표에서는 67%로 감소하고 상대적으로 2층 구조의 비중이 크다. 반시계 방향의 순환과 관련된 북향류의 전형적인 유속은 약 10 cm/s이다. 여기에 중첩되는 변동성으로서 국지적인 바람의 영향은 거의 받지 않는 반면에 동-서 방향의 바람에 의한 외해의 에크만 수송과 관련된 비국지적 영향은 주로 풍속이 강할 경우에 나타난다.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2005년도 춘계학술대회
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• pp.643-646
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• 2005

The gas hydrate exploration using seismic reflection data, the detection of BSR(Bottom Simulating Reflector) on the seismic section is the most important work flow because the BSR have been interpreted as being formed at the base of a gas hydrate zone. Usually, BSR has some dominant qualitative characteristics on seismic section i.e. Wavelet phase reversal compare to sea bottom signal, Parallel layer with sea bottom, Strong amplitude, Masking phenomenon above the BSR, Cross bedding with other geological layer. Even though a BSR can be selected on seismic section with these guidance, it is not enough to conform as being true BSR. Some other available methods for verifying the BSR with reliable analysis quantitatively i.e. Interval velocity analysis, AVO(Amplitude Variation with Offset)analysis etc. Usually, AVO analysis can be divided by three main parts. The first part is AVO analysis, the second is AVO modeling and the last is AVO inversion. AVO analysis is unique method for detecting the free gas zone on seismic section directly. Therefore it can be a kind of useful analysis method for discriminating true BSR, which might arise from an Possion ratio contrast between high velocity layer, partially hydrated sediment and low velocity layer, water saturated gas sediment. During the AVO interpretation, as the AVO response can be changed depend upon the water saturation ratio, it is confused to discriminate the AVO response of gas layer from dry layer. In that case, the AVO modeling is necessary to generate synthetic seismogram comparing with real data. It can be available to make conclusions from correspondence or lack of correspondence between the two seismograms. AVO inversion process is the method for driving a geological model by iterative operation that the result ing synthetic seismogram matches to real data seismogram wi thin some tolerance level. AVO inversion is a topic of current research and for now there is no general consensus on how the process should be done or even whether is valid for standard seismic data. Unfortunately, there are no well log data acquired from gas hydrate exploration area in Korea. Instead of that data, well log data and seismic data acquired from gas sand area located nearby the gas hydrate exploration area is used to AVO analysis, As the results of AVO modeling, type III AVO anomaly confirmed on the gas sand layer. The Castagna's equation constant value for estimating the S-wave velocity are evaluated as A=0.86190, B=-3845.14431 respectively and water saturation ratio is $50\%$. To calculate the reflection coefficient of synthetic seismogram, the Zoeppritz equation is used. For AVO inversion process, the dataset provided by Hampson-Rushell CO. is used.

• Journal of Korean Neurosurgical Society
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• 제63권5호
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• pp.566-578
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• 2020

Objective : Radiation is known to induce autophagy in malignant glioma cells whether it is cytocidal or cytoprotective. Dexamethasone is frequently used to reduce tumor-associated brain edema, especially during radiation therapy. The purpose of the study was to determine whether and how dexamethasone affects autophagy in irradiated malignant glioma cells and to identify possible intervening molecular pathways. Methods : We prepared p53 mutant U373 and LN229 glioma cell lines, which varied by phosphatase and tensin homolog (PTEN) mutational status and were used to make U373 stable transfected cells expressing GFP-LC3 protein. After performing cell survival assay after irradiation, the IC₅₀ radiation dose was determined. Dexamethasone dose (10 μM) was determined from the literature and added to the glioma cells 24 hours before the irradiation. The effect of adding dexamethasone was evaluated by cell survival assay or clonogenic assay and cell cycle analysis. Measurement of autophagy was visualized by western blot of LC3-I/LC3-II and quantified by the GFP-LC3 punctuated pattern under fluorescence microscopy and acridine orange staining for acidic vesicle organelles by flow cytometry. Results : Dexamethasone increased cell survival in both U373 and LN229 cells after irradiation. It interfered with autophagy after irradiation differently depending on the PTEN mutational status : the autophagy decreased in U373 (PTEN-mutated) cells but increased in LN229 (PTEN wild-type) cells. Inhibition of protein kinase B (AKT) phosphorylation after irradiation by LY294002 reversed the dexamethasone-induced decrease of autophagy and cell death in U373 cells but provoked no effect on both autophagy and cell survival in LN229 cells. After ATG5 knockdown, radiation-induced autophagy decreased and the effect of dexamethasone also diminished in both cell lines. The diminished autophagy resulted in a partial reversal of dexamethasone protection from cell death after irradiation in U373 cells; however, no significant change was observed in surviving fraction LN229 cells. Conclusion : Dexamethasone increased cell survival in p53 mutated malignant glioma cells and increased autophagy in PTEN-mutant malignant glioma cell but not in PTEN-wildtype cell. The difference of autophagy response could be mediated though the phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin signaling pathway.

• The Korean Journal of Physiology and Pharmacology
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• 제3권1호
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• pp.1-10
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• 1999

We sought to find out the mechanism of vascular relaxation by extracellular $K^+$ concentration $([K^+]_o)$ in the cerebral resistant arteriole from rabbit. Single cells were isolated from the cerebral resistant arteriole, and using voltage-clamp technique barium-sensitive $K^+$ currents were recorded, and their characteristics were observed. Afterwards, the changes in membrane potential and currents through the membrane caused by the change in $[K^+]_o$ was observed. In the smooth muscle cells of cerebral resistant arteriole, ion currents that are blocked by barium, 4-aminopyridine (4-AP), and tetraethylammonium (TEA) exist. Currents that were blocked by barium showed inward rectification. When the $[K^+]_o$ were 6, 20, 60, and 140 mM, the reversal potentials were $-82.7{\pm}1.0,\;-49.5{\pm}1.86,\;-26{\pm}1.14,\;-5.18{\pm}1.17$ mV, respectively, and these values were almost identical to the calculated $K^+$ equilibrium potential. The inhibition of barium-sensitive inward currents by barium depended on the membrane potential. At the membrane potentials of -140, -100, and -60 mV, $K_d$ values were 0.44, 1.19, and 4.82 ${\mu}M,$ respectively. When $[K^+]_o$ was elevatedfrom 6 mM to 15 mM, membrane potential hyperpolarized to -50 mV from -40 mV. Hyperpolarization by $K^+$ was inhibited by barium but not by ouabain. When the membrane potential was held at resting membrane potential and the $[K^+]_o$ was elevated from 6 mM to 15 mM, outward currents increased; when elevated to 25 mM, inward currents increased. Fixing the membrane potential at resting membrane potential and comparing the barium-sensitive outward currents at $[K^+]_o$ of 6 and 15 mM showed that the barium- sensitive outward current increased at 15 mM $K^+.$ From the above results the following were concluded. Barium-sensitive $K^+$?channel activity increased when $[K^+]_o$ is elevated and this leads to an increase in $K^+-outward$ current. Consequently, the membrane potential hyperpolarizes, leading to the relaxation of resistant arteries, and this is thought to contribute to an increase in the local blood flow of brain.