• 제목/요약/키워드: Fleshy shrimp

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Genetic Differences and DNA Polymorphisms between the Fleshy Prawn Fenneropenaeus chinensis and Chinese Ditch Prawn Palaemon gravieri

  • Yoon Jong-Man;Kim Jong-Yeon
    • Fisheries and Aquatic Sciences
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    • 제8권3호
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    • pp.151-160
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    • 2005
  • Genomic DNA samples isolated from Fenneropenaeus chinensis (fleshy prawn; FP) and Palaemon gravieri (Chinese ditch prawn; CDP) collected in the West Sea, off the Korean Peninsula, at Buan, were PCR-amplified repeatedly. The sizes of the DNA fragments generated by seven different primers varied from 50 bp to 1,600 bp. We identified 358 fragments for the FP species and 301 fragments for the CDP species. There were 18 polymorphic fragments (5.03$\%$) for the FP species and 12 (3.99$\%$) for the CDP species. In total, 66 common fragments (average of 9.4 fragments per primer) were observed for the FP species and 44 fragments (average of 6.3 fragments per primer) were observed for the CDP species. The numbers of specific fragments seen for the FP species and CDP species were 38 and 47, respectively. The complexity of the banding patterns varied dramatically between the primers and the two species. In the FP species, a specific fragment of approximately 1,200 bp generated by primer OPB-04 exhibited inter-individual-specific characteristics that were indicative of DNA polymorphisms. Moreover, in the CDP species, a major fragment of approximately 550 bp generated by primer OPB-20 was found to be specific for the CDP. The average bandsharing value between the two prawn species was 0.421$\pm$0.006, and ranged from 0.230 to 0.611. The dendrogram obtained using the data from the seven primers indicated seven genetic clusters: cluster 1, FLESHY 01, 02, 03, and 04; cluster 2, FLESHY 05, 06, and 07; cluster 3, FLESHY 08, 09, 10, and 11; cluster 4, DITCH 13, 14, 16, and 18; cluster 5, DITCH 12, 15, and 17; cluster 6, DITCH 19, 20, and 21; and cluster 7, DITCH 22. The genetic distance between the two prawn species ranged from 0.071 to 0.642. Thus, RAPD-PCR analysis revealed a significant genetic distance between the two prawn species. Using various arbitrary primers, RAPD-PCR may be applied to identify specific/polymorphic markers that are particular to a species and geographic population, and to define genetic diversity, polymorphisms, and similarities among shrimp species.

Microarray 분석을 이용한 대하 (Fenneropenaeus chinensis) 유생의 카드뮴 단기 노출에 따른 유전자변화 (Acute Toxicity of Cadmium on Gene Expression Profiling of Fleshy Shrimp, Fenneropenaeus Chinensis Postlarvae Using a cDNA Microarray)

  • 김수경;치오궈;윤종화;장인권
    • 한국환경과학회지
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    • 제24권5호
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    • pp.623-631
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    • 2015
  • Microarray technology provides a unique tool for the determination of gene expression at the level of messenger RNA (mRNA). This study, the mRNA expression profiles provide insight into the mechanism of action of cadmium in Fleshy shrimp (Fenneropenaeus chinensis). The ability of genomic technologies was contributed decisively to development of new molecular biomarkers and to the determination of new possible gene targets. Also, it can be approach for monitoring of trace metal using oligo-chip microarray-based in potential model marine user level organisms. 15K oligo-chip for F. chinensis that include mostly unique sets of genes from cDNA sequences was developed. A total of 13,971 spots (1,181 mRNAs up- regulated and 996 down regulated) were identified to be significantly expressed on microarray by hierarchical clustering of genes after exposure to cadmium for different conditions (Cd24-5000 and Cd48-1000). Most of the changes of mRNA expression were observed at the long time and low concentration exposure of Cd48-1000. But, gene ontology analysis (GO annotation) were no significant different between experiments groups. It was observed that mRNA expression of main genes involved in metabolism, cell component, molecular binding and catalytic function. It was suggested that cadmium inhibited metabolism and growth of F. chinensis.

Real-time PCR Quantification of White Spot Syndrome Virus (WSSV) and Hepatopancreatic Parvovirus (HPV) Loads in Shrimp and Seawaters of Shrimp Ponds on the West Coast of South Korea

  • Jang, In-Kwon;Gopalakannan, Ayyaru;Suriakala, Kannan;Kim, Jong-Sheek;Kim, Bong-Rae;Cho, Yeong-Rok;Meng, Xian-Hong;Seo, Hyeong-Chul
    • Fisheries and Aquatic Sciences
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    • 제11권4호
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    • pp.195-204
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    • 2008
  • Viral diseases are major emerging problems of shrimp that have affected the production, and even complete losses for shrimp farms. In this study, we developed a sensitive TaqMan real-time PCR method to quantify white spot syndrome virus (WSSV) and hepatopancreatic parvovirus (HPV) in the shrimp and pond water in which fleshy shrimp, Fenneropenaeus chinensis, and Pacific white shrimp, Litopenaeus vannamei, are reared. WSSV and HPV in pond seawaters ranged from $1.65{\times}10^3$ to $2.43{\times}10^9$ and from 0 to $4.43{\times}10^5$ copies/L of seawater, respectively. Of 20 ponds analyzed, all pond water and shrimp were positive for WSSv. L. vannamei showed higher susceptibility to WSSV than F chinensis. HPV was detected only in the pond water for F chinensis. In shrimp tissue, however, HPV was found in both species, with 23-times higher infection rate in F chinensis than L. vannamei. The total bacterial counts in the pond water ranged from $2.23{\times}l0^3$ to $1.98{\times}l0^5\;CFU/mL$. The variations in total bacterial count for each pond appeared to correlate to the variations of the WSSV load. Statistical analysis indicated that there was no significant difference (P>0.05) between the WSSV load in pond water and shrimp, and there was no relationship between total bacterial load and viral load in the pond water. However, a significant difference (P<0.01) was found between HPV load and L. vannamei and F chinensis pond water.

대하 Penaeidin 3-2 유전자의 동정 및 발현 (Characterization and Expression of Penaeidin 3-2 from Fleshy Prawn Fenneropenaeus chinensis)

  • 박은미;조현국;홍경은;남보혜;김영옥;김우진;이상준;한현섭;이재용;김종식;장인권;정재훈;최태진;공희정
    • 한국해양바이오학회지
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    • 제2권1호
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    • pp.34-39
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    • 2007
  • Penaeidins은 새우류에 존재하는 항미생물성펩티드의 한 종류로서 새우의 외부 병원체에 대한 방어기작을 구성하는 중요한 인자이다. 본 연구에서는 대하 혈구세포의 cDNA library로부터 분리된 EST 클론을 분리 동정하였다. 아미노산 염기서열 분석과 계통수 분석을 통하여 본 연구에서 분리한 EST 클론이 대하의 Penaeidin 3-2 유전자와 아미노산 수준에서 동일함을 밝혔다. 대하의 Penaeidin 3-2는 signal peptides로 예상되는 19개 아미노산 잔기를 포함하여 전체 71개의 아미노산 잔기로 이루어져 있고, C-말단에는 3개의 이중황화결합을 형성할 수 있는 6개의 cysteine가 존재하였다. 대하 Penaeidin 3-2 전사체는 혈구세포, 간췌장, 근육 조직에서 발현되었으며, 특히 혈구세포에서 주로 많이 발현되었다. 흰반점바이러스의 인위감염 실험에서 바이러스 감염 후 대하 Penaeidin 3-2의 발현이 증가하였다. 이상의 결과들로부터 대하 Penaeidin 3-2가 자체 방어 작용에서 중요한 역할을 할 것으로 예상된다.

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축제식 양식장에서의 대하, 흰다리새우와 황복의 복합양식 (Polyculture of Fleshy Shrimp Fenneropenaeus chinensis and White Shrimp Litopenaeus vannamei with River Puffer Takifugu obscurus in Shrimp Ponds)

  • 장인권;전제천;조국진;조영록;서형철;김봉래;김종식
    • 한국양식학회지
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    • 제20권4호
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    • pp.278-288
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    • 2007
  • 새우양식은 90년대 서해안을 중심으로 빠르게 발달하여 2001년에는 2,605ha의 면적에서 3,268톤이 생산되었으나 2004년에는 2,368톤으로 해마다 감소되고 있다. 이러한 원인 중의 하나는 흰반점바이러스(WSSV)에 의한 대량폐사에 기인하는 것으로 알려져 있다. 흰반점바이러스는 현재 세계적으로 새우양식산업에 심각한 피해를 입히는 바이러스로 우리나라에는 1993년 처음 보고된 후 해마다 새우양식장에 반복적인 대량폐사를 유발시킨다. 축제식 새우양식장의 질병 피해를 감소시키기 위한 방법의 하나로서 새우와 어류, 패류, 해조류를 함께 복합양식하는 방법이 많은 연구되어 있으며 또한 육식성 어류와의 복합양식은 질병새우를 선택적으로 포식함으로써 새우의 바이러스 발병을 지연 혹은 억제시키는 것으로 알려져 있다. 새우양식장에 육식성 어류인 황복 Takifugu obscurus을 복합적으로 사육함으로써 바이러스 억제 효과를 조사하기 위하여 수면적 $1,616{\sim}1,848\;m^2$의 4개 축제식 양식장에 각각 흰다리새우($46.9/m^2$), 흰다리새우($43.4/m^2$)+황복($0.22/m^2$), 대하($24.6/m^2$), 대하($30.3/m^2$)+황복($0.25/m^2$)의 밀도로 입식하고 95일간 사육하였다. 대하 단독구와 복합구는 각각 51일, 57일째 WSSV 발병으로 전량 폐사하였다. 흰다리새우 단독구의 생존율은 18.2%인 반면 복합구의 생존율은 32.4%이며 단위생산량은 단독구에 비해 69.2%가 높아 흰다리새우는 황복과 복합양식이 단독양식에 비해 훨씬 유리한 것으로 나타났다. 사육수의 영양염(TAN, $NO_2-N,\;NO_3-N$) 농도는 복합구가 단독구에 비해 2배 이상 높았으나 전체적으로 새우의 성장에 적정범위를 유지하였다. 대하는 흰다리새우에 비해 WSSV에 대한 감수성이 높은 것으로 나타났으며 따라서 대하와의 복합양식은 이러한 점이 충분히 고려되어야 할 것이다.

Comparison of Nucleic Acid Levels, Ratio and Ecophysiological Aspects among Three Populations of the Fleshy Prawn Fenneropenaeus chinensis in Korea

  • Kim Su-Kyoung;Kim Jong-Sheek;Kim Bong-Rae;Kim Dae-Hyun;Cho Yeong-Rok;Seo Hyung-Cheul;Lee Youn-Ho;Kim Jong-Hwa
    • Fisheries and Aquatic Sciences
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    • 제9권1호
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    • pp.7-13
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    • 2006
  • Using biochemical methods, we determined the potential of local female shrimp populations as breeding stock to select the best adult prawns for improving larval production. As condition indexes, we selected total RNA, DNA, their ratio, and trypsin activity. The DNA content in the pleopods of each local population was similar, i.e., between $0.90{\pm}0.06\;and\;1.02{\pm}0.04(SE){\mu}g/mg$. In comparison, the RNA contents differed markedly between $2.00{\pm}0.09$ and $0.96{\pm}0.08\;{\mu}g/mg$. Therefore, the RNA/DNA (R/D) ratio in the pleopod could be used as a condition index because it represents a biochemical characteristic of the population. The mean pleopodal R/D ratio of the Goheung population was the highest at $2.52{\pm}0.19$, which indicated the best condition. Trypsin activity was influenced little by shrimp condition and more by the amount of food ingested. The gonadosomatic index (GSI) and R/D ratio in the gonads provided offsetting information about the instantaneous gonad maturity. The Goheung population had the highest instantaneous GSI, despite some spawning. Based on the condition indexes and time of gonad maturation, the Goheung shrimp population is suitable for use as breeding stock.

사육수 조성에 따른 대하(Fenneropenaeus chinensis)의 공식 및 혈액학적 성상의 변화 (Characterization of cannibalism and blood in fleshy shrimp, Fenneropenaeus chinensis according to rearing water)

  • 김수경;김석렬
    • 한국어병학회지
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    • 제35권2호
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    • pp.185-193
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    • 2022
  • This study investigated carnival behavior in the nursery stage of Fenneropenaeus chinensis. In order to suppress the carnival behavior and improve the survival rate, it was examined whether there was a carnivalism inhibitory effect according to the rearing water of shrimp. In addition, their blood physiological changes were observed. As a result, in the experimental group with the size difference of F. chinensis, the survival rate was the highest in the biofloc technology (BFT) rearing water of olive flounder (75%), and in the experimental group without the size difference, the survival rate was the highest in the seawater experiment group (93%). In both experimental groups, rate of carnival behavior was observed to be low in the fed experimental group regardless of the size difference of F. chinensis. As a result of blood cell analysis of F. chinensis according to the rearing water, the percentage of granulocytes was the highest in the BFT rearing water of flounder (75%) and the lowest in the filtered seawater group (66%). The proportion of semi-granulocytes was the highest at 11% in the shrimp BFT rearing water, and the lowest at 7% in the filtered seawater. The proportion of hyalinocytes was highest in filtered seawater (27%) and lowest in flounder BFT rearing water (16%). These results suggest that carnival behavior and blood composition of F. chinensis may be different depending on the conditions of the rearing water in the nursery stage.

대하 (Penaeus chinensis)의 Zoea와 Mysis기 유생의 성장과 생존율에 미치는 환경요인 및 먹이생물의 영향 (Effects of Environmental Factors and Live Food on Growth and Survival Rate of Zoea and Mysis Larvae of Fleshy Shrimp, Penaeus chinensis)

  • 김현준;허성범
    • 한국양식학회지
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    • 제6권3호
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    • pp.159-171
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    • 1993
  • 대하의 2oea와 mysis 기의 성장과 생존에 대한 밀도, 빛, 수온, 먹이의 영향은 다음의 조사결과와 같다. 사육 밀도에 따른 유생의 생존율은 500마리/l에서 보다 200, 300마리/l일때 휠씬 높았고, 사육밀도 100, 200, 300, 500마리/l일때 생존율은 90.3, 95.2, 94.3, $86.7\%$ 이었다. 규조를 혼합하여 먹이로 공급한 광주기 실험에서는 24시간 연속 조명하였을 때 생존율이 $96.5\%$로 자연광을 이용할 때 $(79.0\%)$와 24시간 암흑 처리할 때 $(4.3\%)$에 비해 매우 높은 생존과 빠른 성장을 보였다. 사육 수온은 $19^{\circ}C$에서 $97.3\%$의 생존을 보였지만 $22^{\circ}C$에 비해 성장이 늦었으며, $22^{\circ}C$에서 $96.7\%$ 의 생존율과 빠른 성장을 보였다. 수온 $25^{\circ}C$에서는 $51.6\%$의 생존율로 $19,\;22^{circ}C$에 비해 생존율과 성장이 저조하였다. 먹이생물로는 6종의 규조(A. normaii, C. nomaii, C. simplex, N. clostuium, P tricomutum, S. costatum, T weissflogii)를 혼합하여 공급하였을 때, 연속 조명으로 사육할 경우, $97.3\%$의 가장 높은 생존율과 최고의 성장을 나타내었으며, 1종의 규조만을 먹이생물로 공급하였을 때에는 S. costatum을 공급하였을 때의 생존율 $82.1\%$, T. weissflogii의 $80.6\%$ 를 제외하고는 모두 $62\%$이하의 생존율과 저조한 성장을 나타내었다.

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Relationship between White Spot Symptom and Physiological Status of Two Penaeid Shrimps

  • Kim, Su Kyoung;Kim, Myung Seok;Park, Myoung Ae;Kim, Su mi;Jang, In Kwon;Kim, Seok Ryel;Cho, Miyoung
    • 환경생물
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    • 제35권4호
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    • pp.461-467
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    • 2017
  • Shrimps infected with WSSV(White Spot Syndrome Virus) generally exhibit white spots in their inner space of carapaces as an acute clinical sign. In an effort to identify the correlation between this acute clinical sign and the condition, the index factors (RNA/DNA concentration and ratio, trypsin activity) were analyzed. A total 580 farmed Fenneropenaeus chinensis and 130 Lithopenaeus vannamei were collected from western and southern fifteen outdoor ponds in Korea. The status of the white spot pathology was divided into four stages (stage 0, stage I, stage II, and stage III), in accordance with the clinical signs as to the size and area of white spots. A significant decrease in RNA concentration and RNA/DNA ratio for multi-infected fleshy prawn (WSSV and vibrio sp.) occurred during the stage III (the whole carapace is covered with a white spot). In particular, RNA/DNA ratio was significantly lower as $1.47{\pm}0.04$ than other groups. A similar trend was also found in the single infection (WSSV), but the decrease was less than the multi-infection. In the species comparison, both species were vulnerable to the multi-infection, but L. vannamei was more sensitive than F. chinensis(ANOVA, p<0.05): A significant decrease in RNA concentration and RNA/DNA ratio was first found in stage II for the former species, while it was found in stage III for the latter species. Trypsin activity was also showed a similar tendency with nucleic acid variation. Multi-infected shrimp showed drastically decrease of trypsin activity. According to the results, clinical signs of the white spot under carapace have an only physiological effect on shrimp if they covered entirely with white spots.

사육수 비교환 방식에 의한 흰다리새우의 고밀도 사육 I. 후기유생(postlarva)의 실내 중간육성 (Intensive Culture of the Pacific White Shrimp Litopenaeus vannamei under Limited Water Exchange I. Indoor Nursery Culture of Postlarvae)

  • 장인권;김종식;조국진;서형철;조영록;;김봉래
    • 한국양식학회지
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    • 제21권4호
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    • pp.339-345
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    • 2008
  • 서해안의 주요 양식종인 대하는 최근 바이러스를 포함한 질병으로 인하여 생산성이 크게 저하되고 있다. 이에 대한 대책으로 2003년 이식된 흰다리새우는 급속도로 양식이 확산되어 2007년 전국새우양식 생산량의 62.5%를 점유하였으나 흰다리새우의 특성에 맞는 양식기술은 거의 연구되지 않았다. 새우유생의 중간육성은 생존율과 사료효율의 향상, 생산량 증대, 바이러스성 질병피해 감소 뿐 아니라 양성기간의 단축 등의 장점이 있다. 본 연구에서는 사육수 비교환 방식으로 시설된 4개의 raceway 수조에 흰다리새우 postlarva (${PL_3}-{PL_{10}}$)를 $3,750-9,090/m^3$의 밀도로 입식하고 중간육성을 실시하였다. 35일의 사육 결과, 생존율은 $4,090/m^3$ 밀도의 수조에서 93.6%로 가장 높았으며, $9,090/m^3$의 tank에서 58.1로 가장 낮았다. 수확시 평균 체중은 0.071-0.108 g이었으며 FCR은 0.59-0.70으로 매우 낮았다. TAN농도는 사육 10일째 전 tank에서 20 ppm까지 상승한 이후 점차 감소하여 3주째부터 크게 낮아졌으며 아질산염 농도는 3주째부터 급격하게 상승하여 타가영양상태로의 전환이 관찰되었다. 본 연구 결과는 일부 시스템의 보완과 규모의 확대를 통하여 양식현장에서 직접 응용 가능할 것으로 기대된다.